发酵方式对苎麻韧皮生物脱胶效果的影响
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摘要
为了进一步明确合适的生物脱胶发酵方式,研究利用高效菌株DCE01通过湿润和浸泡发酵方式对苎麻韧皮进行生物脱胶,从0.5 h开始计时直至10.5 h结束,每隔2 h取样一次,结合半自动纤维分析仪法和柱前衍生-高效液相色谱法对苎麻韧皮生物脱胶产物中的半纤维素、纤维素、木质素和水解液中半乳糖醛酸含量进行测定。结果表明:10.5 h脱胶终止时,浸泡发酵苎麻韧皮生物脱胶产物中半纤维素、木质素、纤维素和水解液半乳糖醛酸含量分别为4.28%、0.11%、91.88%、4.93μg/m L;湿润发酵的相应含量为4.55%、0.13%、91.56%、5.03μg/m L。整个脱胶期间,两种发酵方式生物脱胶产品中的半纤维素、木质素、半乳糖醛酸含量均随脱胶时间的延长不断下降,纤维素含量不断上升,并经方差齐性F检验,表明两种发酵方式脱胶效果无显著差异。
To clarify a suitable bio-degumming fermentation method,wet fermentation and immersion fermentation methods were carried out in ramie bio-degumming by strain DCE01. The bio-degumming products including hemicellulose,cellulose,lignin and galacturonic acid were detected by Semi-automatic Fiber Analyzer and Pre-column Derivatization High Performance Liquid Chromatography. The samples were taken every 2 hours from 0.5 h to 10.5 h. The results showed that the contents of hemicellulose,lignin,cellulose and galacturonic acid in ramie wet bio-degumming was 4.28%,0.11%,91.88%and 4.93 μg/m L respectively at the end of 10.5 h,and in wet fermentation was 4.55%,0.13%,91.56%and 5.03 μg/m L. During the degumming,the contents of hemicellulose,lignin and galacturonic acid in the two fermentation methods decreased with the prolongation of degumming time,and the content of cellulose increased. The variance F test showed that there was no significant difference in two degumming methods.
To clarify a suitable bio-degumming fermentation method,wet fermentation and immersion fermentation methods were carried out in ramie bio-degumming by strain DCE01. The bio-degumming products including hemicellulose,cellulose,lignin and galacturonic acid were detected by Semi-automatic Fiber Analyzer and Pre-column Derivatization High Performance Liquid Chromatography. The samples were taken every 2 hours from 0.5 h to 10.5 h. The results showed that the contents of hemicellulose,lignin,cellulose and galacturonic acid in ramie wet bio-degumming was 4.28%,0.11%,91.88%and 4.93 μg/m L respectively at the end of 10.5 h,and in wet fermentation was 4.55%,0.13%,91.56%and 5.03 μg/m L. During the degumming,the contents of hemicellulose,lignin and galacturonic acid in the two fermentation methods decreased with the prolongation of degumming time,and the content of cellulose increased. The variance F test showed that there was no significant difference in two degumming methods.
引文
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[2]郭双庆,谢光银.苎麻纤维生物脱胶[J].广西纺织科技,2009,38(6):17-19.
[3]郑皆德,刘让同,李玉玲,等.苎麻酶法脱胶新工艺的应用研究[J].纺织学报,2002,23(4):314-315.
[4]Zhou C,Xue Y,Ma Y. Cloning,evaluation,and high-level expression of a thermo-alkaline pectate lyase from alkaliphilic Bacillus clausii with potential in ramie degumming[J]. Applied Microbiology and Biotechnology,2017,101(9):3663-3676.
[5]Zhou Z,Liu Y,Chang Z,et al. Structure-based engineering of a pectate lyase with improved specific activity for ramie degumming[J]. Applied Microbiology and Biotechnology,2017,101(7):2919-2929.
[6]薛小华,丁若垚,郁崇文,等.亚麻脱胶菌发酵条件的优化及酶的粗提[J].中国麻业科学,2013,35(4):185-190.
[7]刘正初,孙庆祥.草本纤维生物精制科学与工程[M].北京:中国农业科学技术出版社,2018.
[8]樊培.苎麻纤维原位生物脱胶制造技术研究与应用[D].湖北:华中科技大学,2015.
[9]罗远莉.苎麻生物脱胶的研究[D].重庆:重庆大学,2007.
[10]冯湘沅,成莉凤,段盛文,等. DCE01菌株降解大麻韧皮的发酵液成分变化规律[J].中国麻业科学,2016,38(5):202-206.
[11]王加启,于建国,吴克谦,等.饲料中中性洗涤纤维的测定[S].北京:中国标准出版社,2007.
[12]刘文静,潘葳,任丽花.FIBERTEC 2010半自动纤维分析仪测饲料中纤维素、半纤维素、木质素的方法研究[J].福建农业学报,2013,28(7):722-726.
[13]冯湘沅,成莉凤,段盛文,等.苎麻水解液中单糖类组分的柱前衍生—高效液相色谱法检测[J].纺织学报,2015,36(12):16-19.
[14]姜彩霞,刘晓兰,田黄华,等.高效液相色谱法测定亚麻纤维果胶单糖衍生条件的优化[J].毛纺科技,2014,42(10):1-4.