紫外分光光度法测定丁香油膜剂丁香酚含量的方法学研究
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摘要
目的对丁香油口腔膜剂中有效成分丁香酚进行含量测定。方法建立丁香油口腔膜剂中丁香酚含量的紫外分析方法。结果该方法简单、准确、重现性好。丁香酚在14.85~39.60μg/ml范围内线性关系良好,r=0.9999,平均加样回收率为98.6%,RSD=2.6%。结论采用紫外分光光度法测定丁香油口腔膜剂中丁香酚含量可有效进行质量控制,为下一步开展药效实验奠定较好的研究基础。
Objective To build determination methods of eugenol that is the active ingredients of clove oil oral ulcer film. Methods To establish a UV method for quality control of clove oil oral ulcer film. Results The method for determination of eugenol by UV was simple,accurate and reproducible. Eugenol in the range of 14. 85 ~ 39. 60μg / ml was linear,r = 0. 9999,average recovery was 98. 6%,and RSD = 2. 6 %. Conclusion To made the quality evaluation of clove oil oral ulcer film by UV,and lay a good foundation for further research of efficacy experiments.
Objective To build determination methods of eugenol that is the active ingredients of clove oil oral ulcer film. Methods To establish a UV method for quality control of clove oil oral ulcer film. Results The method for determination of eugenol by UV was simple,accurate and reproducible. Eugenol in the range of 14. 85 ~ 39. 60μg / ml was linear,r = 0. 9999,average recovery was 98. 6%,and RSD = 2. 6 %. Conclusion To made the quality evaluation of clove oil oral ulcer film by UV,and lay a good foundation for further research of efficacy experiments.
引文
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[10]陈新国,董云平,黄俊忠.GC法测定琥珀止痛膏中樟脑、薄荷脑、冰片、八角茴香油、桂皮醛和丁香酚的含量[J].药物分析杂志,2013,33(9):1603.
[11]张忠,任飞,张盼.液-液萃取-液相色谱-串联质谱法测定“地沟油”中辣椒碱类化合物及丁香酚[J].色谱,2012,30(11):1108.
[12]邝婉容,刘芳,杜卓,等.丁香油口腔膜剂的制备与质量评价[J].山东化工,2015,44(16):22.
[13]张瑱,陈钧,陈剑,等.丁香酚痤疮凝胶剂的处方设计及初步质量评价[J].时珍国医国药,2006,17(1):23.
[2]俞懿强.丁香酚的药理作用及在口腔医学应用的研究进展[J].临床口腔医学杂志,2009,25(3):187.
[3]李剑勇,王棋文,于远光,等.阿司匹林丁香酚酯的抗炎作用及其可能的作用机制[J].中国药理学与毒理学杂志,2011,25(1):57.
[4]Moreira-Lobo DC,Linhares-Siqueira ED,Cruz GM,et al.Eugenol modifies the excitability of rat sciatic nerve and superior cervical ganglion neurons[J].Neurosci Lett,2010,472(3):220.
[5]Kurian R,Arulmozhi DK,Veeranjaneyulu A,et al.Effect of eugenol on animal models of noceception[J].Indian J Pharmacol,2006,38(5):341.
[6]张冬.丁香油冰硼散糊剂对复发性口腔溃疡疼痛的缓解作用[J].中国中医药信息杂志,2008,15(10):65.
[7]臧亚茹,蔡振岭,赖光辉,等.复方丁香胶浆治疗口腔溃疡的疗效观察[J].实用口腔医学杂志,2006,22(4):495.
[8]沙延淳,杨红玉,石莉,等.丁香口含片的研制[J].辽宁中医杂志,2013,40(8):1668.
[9]黎迎,陆洋,杜守颖,等.对《中国药典》2010年版丁香药材中丁香酚含量测定方法的探讨[J].中药新药与临床药理,2012,23(5):579.
[10]陈新国,董云平,黄俊忠.GC法测定琥珀止痛膏中樟脑、薄荷脑、冰片、八角茴香油、桂皮醛和丁香酚的含量[J].药物分析杂志,2013,33(9):1603.
[11]张忠,任飞,张盼.液-液萃取-液相色谱-串联质谱法测定“地沟油”中辣椒碱类化合物及丁香酚[J].色谱,2012,30(11):1108.
[12]邝婉容,刘芳,杜卓,等.丁香油口腔膜剂的制备与质量评价[J].山东化工,2015,44(16):22.
[13]张瑱,陈钧,陈剑,等.丁香酚痤疮凝胶剂的处方设计及初步质量评价[J].时珍国医国药,2006,17(1):23.