气相色谱法测定舒筋理伤油中4种有效成分的含量
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摘要
目的建立舒筋理伤油中冰片、薄荷脑、樟脑、水杨酸甲酯的气相色谱测定方法。方法采用毛相色谱法,使用DB-WAXETR弹性石英毛细管柱,FID检测器,检测器温度:250℃,进样口温度:230℃,柱温:程序升温,初始温度100℃,以10℃·min-1的速率升温,升至210℃后保持6 min。结果冰片、薄荷脑、樟脑、水杨酸甲酯浓度在0.078 70~1.967、0.043 12~1.078、0.041 79~1.045、0.8319~20.80 mg·m L-1内与峰面积呈良好的线性关系,平均回收率(n=6)分别为98.2%、97.4%、99.0%和99.7%,RSD分别为1.7%、1.8%、1.8%和1.6%。结论所建立的方法快速、稳定、可靠,可作为舒筋理伤油的质量控制标准。
Objective To establish a GC method to determine synthetic borneol, menthol, camphor and methyl salicylate in Shujin Lishang oil. Methods GC was performed on a DB-WAXETR quartz capillary column. Flame ionization detector(FID) was used in the determination. The detector temperature was 250 ℃, and the injector temperature was 230 ℃. The program temperature of the column was as follows: initial temperature at 100 ℃, then raised to 210 ℃ at a rate of 10 ℃·min-1, lasting 6 min. Results Good linearity was found at 0.078 70-1.967 mg·m L-1 for borneol, 0.043 12-1.078 mg·m L-1 for menthol, 0.041 79-1.045 mg·m L-1 for camphor, and 0.8319-20.80 mg·m L-1 for methyl salicylate. The average recoveries of borneol, enthol, camphor and methyl salicylate(n = 6) were 98.2%, 97.4%, 99.0% and 99.7%, respectively. The RSDs were 1.7%, 1.8%, 1.8% and 1.6%, respectively. Conclusion The method is rapid, steady and reliable. It can be used as the quality standard of Shujin Lishang oil.
Objective To establish a GC method to determine synthetic borneol, menthol, camphor and methyl salicylate in Shujin Lishang oil. Methods GC was performed on a DB-WAXETR quartz capillary column. Flame ionization detector(FID) was used in the determination. The detector temperature was 250 ℃, and the injector temperature was 230 ℃. The program temperature of the column was as follows: initial temperature at 100 ℃, then raised to 210 ℃ at a rate of 10 ℃·min-1, lasting 6 min. Results Good linearity was found at 0.078 70-1.967 mg·m L-1 for borneol, 0.043 12-1.078 mg·m L-1 for menthol, 0.041 79-1.045 mg·m L-1 for camphor, and 0.8319-20.80 mg·m L-1 for methyl salicylate. The average recoveries of borneol, enthol, camphor and methyl salicylate(n = 6) were 98.2%, 97.4%, 99.0% and 99.7%, respectively. The RSDs were 1.7%, 1.8%, 1.8% and 1.6%, respectively. Conclusion The method is rapid, steady and reliable. It can be used as the quality standard of Shujin Lishang oil.
引文
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[8]彭敏,邓楠,刘文,等.GC-MS法同步测定复方麝香注射液中麝香酮、β-细辛醚、百秋李醇、龙脑、薄荷脑[J].药物分析杂志,2014,34(5):855-858.
[9]颜敏,刘园园,黄海萍.GC-MS法定性定量分析贴敷类医疗器械中樟脑、薄荷脑、冰片[J].中国药师,2012,15(2):167-169.
[10]白林,范金钊,徐风华,等.HPLC法测定复方樟脑软膏中樟脑的含量[J].中国药物应用与监测,2014,11(4):212-213.
[11]马金刚,危雪平,王鹏远,等.UHPLC法同时测定复方丁香油涂剂中丁香酚和樟脑的含量[J].解放军药学学报,2014,30(5):423-424.
[2]中国药典2010年版.一部[S].2010:136-137,702.
[3]李本俊,才凤,赵华,等.羚羊清肺颗粒中冰片的含量测定[J].中国医药导报,2014,11(26):81-83.
[4]文庆,聂平,张帆,等.气相色谱法同时检测附桂风湿膏中薄荷脑、冰片和水杨酸甲酯的含量[J].中南药学,2014,12(6):585-588.
[5]朱静,贾晓冰,郑智音,等.挥发性成分在橡胶膏剂处方中的作用及如何提供其稳定性[J].中华中医药杂志,2012,27(5):1360-1364.
[6]隆颖,栗建明.正红花油的质量标准研究[J].今日药学,2011,21(2):103-105.
[7]兰文,刘雁鸣,石笑弋.气相色谱法测定樟脑及苯酚的含量[J].中南药学,2013,11(12):931-933.
[8]彭敏,邓楠,刘文,等.GC-MS法同步测定复方麝香注射液中麝香酮、β-细辛醚、百秋李醇、龙脑、薄荷脑[J].药物分析杂志,2014,34(5):855-858.
[9]颜敏,刘园园,黄海萍.GC-MS法定性定量分析贴敷类医疗器械中樟脑、薄荷脑、冰片[J].中国药师,2012,15(2):167-169.
[10]白林,范金钊,徐风华,等.HPLC法测定复方樟脑软膏中樟脑的含量[J].中国药物应用与监测,2014,11(4):212-213.
[11]马金刚,危雪平,王鹏远,等.UHPLC法同时测定复方丁香油涂剂中丁香酚和樟脑的含量[J].解放军药学学报,2014,30(5):423-424.