复方中草药制剂对早期断奶仔猪蛋白质合成能力及抗应激能力的影响
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摘要
本试验旨在研究复方中草药制剂对早期断奶仔猪蛋白质合成能力及抗应激能力的影响。选用平均体重为(7.27±0.18)kg、断奶日龄为(28±2)d的长白猪×约克夏猪仔猪126头,采用单因素完全随机区组试验设计,分为7组,每组3个重复,每个重复6头猪。日粮中中草药复方制剂剂型为水煎剂和散剂2种。试验1组为空白对照组;试验2、3、4组为水煎剂添加组,添加量分别为0.05%、0.10%、0.15%;试验5、6、7组为散剂添加组,添加量分别为0.05%、0.10%、0.15%。试验期21d。结果表明,与对照组相比,复方中草药制剂可显著提高早期断奶仔猪血清中碱性磷酸酶(AKP)、谷草转氨酶(GOT)、谷丙转氨酶(GPT)的活力及总蛋白(TP)、白蛋白(ALB)的含量(P<0.05),显著降低血清中尿素氮(BUN)含量(P<0.05);显著降低早期断奶仔猪血清中肌酸激酶(CK)活力及葡萄糖(GLU)含量(P<0.05)。综上所述,在本试验条件下,复方中草药制剂能显著促进早期断奶仔猪机体蛋白质的合成,降低断奶仔猪的应激,且中草药剂型散剂优于水煎剂,最适添加比例为0.15%。
This experiment was conducted to study the effect of Chinese herbal formula supplementation on protein synthesis and anti-stress ability of early weaned piglets.126 crossbred piglets(Landrace×Yorkshire)weaned at(28±2)days of age were randomly assigned into 7groups with 3replicates per group and 6piglets per replicate.Piglets in group 1was control group which fed a basal diet,piglets in groups 2,3,4were fed diets supplemented with 0.05%,0.10%,0.15% water decoctum of Chinese herbal foumula,respectively,piglets in groups 5,6,7were fed diets supplemented with 0.05%,0.10%,0.15%powders of Chinese herbal foumula,respectively.The experiment lasted for 21 days.The results showed that compared with the control group,Chinese herbal compound additives had significantly increased the activities of serum AKP,GOT,GPT and the contents of serum TP,ALB of early weaned piglets(P<0.05),but had significantly decreased the content of serum BUN of early weaned piglets(P<0.05).Chinese herbal compound additives had significantly decreased the activity of serum CK and the content of serum GLU of early weaned piglets(P<0.05).In conclusion,under this experiment conditions,Chinese herbal compound additives could promote the protein synthesis of early weaned piglets,reduce the anti-stress of weaned piglets,and powder was better than the water decoctum of Chinese herbal compound additives form,the appropriate supplemental level was 0.15%.
This experiment was conducted to study the effect of Chinese herbal formula supplementation on protein synthesis and anti-stress ability of early weaned piglets.126 crossbred piglets(Landrace×Yorkshire)weaned at(28±2)days of age were randomly assigned into 7groups with 3replicates per group and 6piglets per replicate.Piglets in group 1was control group which fed a basal diet,piglets in groups 2,3,4were fed diets supplemented with 0.05%,0.10%,0.15% water decoctum of Chinese herbal foumula,respectively,piglets in groups 5,6,7were fed diets supplemented with 0.05%,0.10%,0.15%powders of Chinese herbal foumula,respectively.The experiment lasted for 21 days.The results showed that compared with the control group,Chinese herbal compound additives had significantly increased the activities of serum AKP,GOT,GPT and the contents of serum TP,ALB of early weaned piglets(P<0.05),but had significantly decreased the content of serum BUN of early weaned piglets(P<0.05).Chinese herbal compound additives had significantly decreased the activity of serum CK and the content of serum GLU of early weaned piglets(P<0.05).In conclusion,under this experiment conditions,Chinese herbal compound additives could promote the protein synthesis of early weaned piglets,reduce the anti-stress of weaned piglets,and powder was better than the water decoctum of Chinese herbal compound additives form,the appropriate supplemental level was 0.15%.
引文
1 马玉芳,刘建成,黄一帆,等.中药饲料添加剂对仔猪生长性能和免疫功能的影响[J].福建农林大学学报,2003,32(4):496~499.
2 孔祥峰,印遇龙,黄瑞林,等.中药超微粉饲料添加剂对断奶仔猪生长性能和血清生化参数的影响[J].中国兽医学报,2008,28 (2):184~188.
3 王树华,赵晓勇.猪饲料中草药添加剂的研究[J].河北畜牧兽医,1999,15(5):42.
4 王吉桥,祁彩霞,程爱香,等.黄芪、熟地和山楂等中草药对黄颡鱼生长和消化的影响[J].水产学杂志,2008,21(1):34~41.
5 李玉,陈亮,孟庆娟,等.复方中药制剂对仔猪生长性能及免疫机能的影响[J].中国畜牧兽医,2011,38(10):9~13.
6 李清宏,武晋孝,韩俊文,等.复方中草药添加剂对仔猪饲料代谢动力学的研究[J].中国农学通报,2007,23(3):19~23.
7 陈琼,廖三赛,贺长青,等.抗仔猪断奶应激剂对断奶仔猪血清生化指标及血常规的影响[J].广西畜牧兽医,2008,24(3):631 ~931.
8 陈晓明,李肖梁,汤丽萍,等.中草药渣对断奶仔猪生长、肠道菌群和免疫的影响[J].浙江农业学报,2007,19(1):50~54.
9 陈玉春,顾雪飞,刘敏.5种中草药对鲤鱼血清谷丙转氨酶、谷草转氨酶及红细胞过氧化氢酶活性的影响[J].淡水渔业,2007,37 (5):11~13.
10 费初林,陆建良,姚小玲.中草药复合饲料添加剂的研究与应用[J].上海畜牧兽医通讯,1999,4:12~13.
11 夏新山,袁书林,杨元青,等.中草药添加剂及不同饲粮类型对生长育肥猪血液生化指标的影响[J].动物科学与动物医学,2003,20 (12):38~39.
12 童晓莉,刘宗慧,郑小波,等.不同添加剂预混料对哺乳仔猪生产性能的影响[J].四川畜牧兽医学院学报,2002,16(1):10~14.
13 解洪业,范玉霞,田生华,等.中草药添加剂对仔猪生长性能的影响[J].青海畜牧兽医杂志,2001,31(2):6~7.
14 Abraham M R,Selivanov V A,Hodgson D M,et al.Coupling of cell energetics with membrane metabolic sensing.Integrative signaling through creating kinase phosphotransfer disrupted by MCK gene knock-out[J].J Biol Chem,2002,277(27):24427~24434.
15 Konno C,Suzuki Y,Oishi K,et al.Isolation and hypoglycemic activity of atractans A,B and C,glycans of Atractylodes japonica rhizomes[J].Planta Med,1985,51(2):102~103.
16 Spindler M,Niebler R,Remkes H,et al.Mitochondrial creatine kinase is critically necessary for normal myocardial high-energy phosphate metabolism[J].American Journal of Physiology-Heart and Circulatory Physiology,2002,283(2):680~687.
2 孔祥峰,印遇龙,黄瑞林,等.中药超微粉饲料添加剂对断奶仔猪生长性能和血清生化参数的影响[J].中国兽医学报,2008,28 (2):184~188.
3 王树华,赵晓勇.猪饲料中草药添加剂的研究[J].河北畜牧兽医,1999,15(5):42.
4 王吉桥,祁彩霞,程爱香,等.黄芪、熟地和山楂等中草药对黄颡鱼生长和消化的影响[J].水产学杂志,2008,21(1):34~41.
5 李玉,陈亮,孟庆娟,等.复方中药制剂对仔猪生长性能及免疫机能的影响[J].中国畜牧兽医,2011,38(10):9~13.
6 李清宏,武晋孝,韩俊文,等.复方中草药添加剂对仔猪饲料代谢动力学的研究[J].中国农学通报,2007,23(3):19~23.
7 陈琼,廖三赛,贺长青,等.抗仔猪断奶应激剂对断奶仔猪血清生化指标及血常规的影响[J].广西畜牧兽医,2008,24(3):631 ~931.
8 陈晓明,李肖梁,汤丽萍,等.中草药渣对断奶仔猪生长、肠道菌群和免疫的影响[J].浙江农业学报,2007,19(1):50~54.
9 陈玉春,顾雪飞,刘敏.5种中草药对鲤鱼血清谷丙转氨酶、谷草转氨酶及红细胞过氧化氢酶活性的影响[J].淡水渔业,2007,37 (5):11~13.
10 费初林,陆建良,姚小玲.中草药复合饲料添加剂的研究与应用[J].上海畜牧兽医通讯,1999,4:12~13.
11 夏新山,袁书林,杨元青,等.中草药添加剂及不同饲粮类型对生长育肥猪血液生化指标的影响[J].动物科学与动物医学,2003,20 (12):38~39.
12 童晓莉,刘宗慧,郑小波,等.不同添加剂预混料对哺乳仔猪生产性能的影响[J].四川畜牧兽医学院学报,2002,16(1):10~14.
13 解洪业,范玉霞,田生华,等.中草药添加剂对仔猪生长性能的影响[J].青海畜牧兽医杂志,2001,31(2):6~7.
14 Abraham M R,Selivanov V A,Hodgson D M,et al.Coupling of cell energetics with membrane metabolic sensing.Integrative signaling through creating kinase phosphotransfer disrupted by MCK gene knock-out[J].J Biol Chem,2002,277(27):24427~24434.
15 Konno C,Suzuki Y,Oishi K,et al.Isolation and hypoglycemic activity of atractans A,B and C,glycans of Atractylodes japonica rhizomes[J].Planta Med,1985,51(2):102~103.
16 Spindler M,Niebler R,Remkes H,et al.Mitochondrial creatine kinase is critically necessary for normal myocardial high-energy phosphate metabolism[J].American Journal of Physiology-Heart and Circulatory Physiology,2002,283(2):680~687.