MMP-7在婴幼儿血管瘤中的表达及意义
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摘要
目的研究基质金属蛋白酶-7(MMP-7)在增生期、消退期婴幼儿血管瘤及正常皮肤组织中表达情况,并探讨其临床意义。方法采用即用型快捷免疫组化MaxVision/HRP检测法检测MMP-7、Ki67在增生期血管瘤(20例)、消退期血管瘤(20例)及正常皮肤(20例)表达情况;夹心法酶联免疫吸附法(ELISA)检测增生期血管瘤患者血清(36例)与正常对照组(36例)血清中MMP-7的表达情况。结果 MMP-7在增生期婴幼儿血管瘤中高表达,在消退期弱阳性表达,在正常皮肤中不表达。增生期与消退期、增生期与对照组比较差异均有统计学意义(P<0.05);Ki67在增生期高表达,消退期低表达,在正常组织中不表达或弱表达,增生期与消退期、增生期与正常组织比较差异有统计学意义(P<0.05);MMP-7在血管瘤患儿血清中的表达水平较正常对照组高,两者比较差异有统计学意义(P<0.05)。结论 MMP-7在血管瘤中有阳性表达,其不同表达水平可能和血管瘤发生发展消退有关。
Objective To study the expression of matrix metalloproteinase-7(MMP-7) in infantile hemangioma and normal skin tissue in the proliferative and regressive stages,and further explore its clinical significance.Methods MaxVision/HRP was used to detect the expression of MMP-7 and Ki67 in proliferative hemangioma(20 cases),regression hemangioma(20 cases) and normal skin(20 cases).The expression of MMP-7 in serum of 36 patients with hemangioma at the proliferative stage and the control group(36 patients) was detected by Sandwich ELISA.Results MMP-7 was highly expressed in infantile hemangioma in the proliferative phase and weakly positive in the regression phase,while no expression was shown in normal skin.There were statistically significant differences between the hyperplasia phase and the regression phase and between the hyperplasia phase and the control group.Ki67 was highly expressed in the proliferative phase,but was poorly expressed in the regression phase,and was not expressed in normal tissues.The difference of Ki67 expression between the proliferative phase and the regression phase,and between the proliferative phase and normal tissues was statistically significant.The expression of MMP-7 in serum of children with hemangioma was higher than that in the normal control group.Conclusion MMP-7 is positively expressed in hemangioma,and its different expression might be related to the occurrence,development and regression of hemangioma.
Objective To study the expression of matrix metalloproteinase-7(MMP-7) in infantile hemangioma and normal skin tissue in the proliferative and regressive stages,and further explore its clinical significance.Methods MaxVision/HRP was used to detect the expression of MMP-7 and Ki67 in proliferative hemangioma(20 cases),regression hemangioma(20 cases) and normal skin(20 cases).The expression of MMP-7 in serum of 36 patients with hemangioma at the proliferative stage and the control group(36 patients) was detected by Sandwich ELISA.Results MMP-7 was highly expressed in infantile hemangioma in the proliferative phase and weakly positive in the regression phase,while no expression was shown in normal skin.There were statistically significant differences between the hyperplasia phase and the regression phase and between the hyperplasia phase and the control group.Ki67 was highly expressed in the proliferative phase,but was poorly expressed in the regression phase,and was not expressed in normal tissues.The difference of Ki67 expression between the proliferative phase and the regression phase,and between the proliferative phase and normal tissues was statistically significant.The expression of MMP-7 in serum of children with hemangioma was higher than that in the normal control group.Conclusion MMP-7 is positively expressed in hemangioma,and its different expression might be related to the occurrence,development and regression of hemangioma.
引文
[1] 马志军,李洁.Ki67与肿瘤关系的研究发展[J].中国医方前沿,2012,7(7):15-16.
[2] Sadlecki P,Bodnar M,Grabiec M,et al,The role of Hypoxia-Inducible factor-1α glucose transporter-1,(GLUT-1)and carbon anhydrase IX in endometrial cancer patient[J].Biomed Res Int,2014,2014:616850.
[3] Vosseler S,Lederle W,Airola K,et al.Distinct progression-associated expression of tumor and stromal MMPs in HaCaT skin SCCs correlates with onset of invasion[J].Int J Cancer,2009,125(10):2296-2306.
[4] Wu T,Li Y,Lu J,et al.Increased MMP-21 expression is associated with poor overall survival of patients with gastric cancer[J].Med Oncol,2013,30(1):323.
[5] Shi H,Liu L,Liu L M,et al.Inhibition of tumor growth by β-elemene through downregulation of the expression of uPA,uPAR,MMP-2,and MMP-9 in a murine intraocular melanoma model[J].Melanoma Res,2015,25(1):15-21.
[6] Shi C H,Wang W S,Zhang Z D,et al.Effect of lentivirus-mediated uPA silencing on the proliferation and apoptosis of chondrocytes and the expression of MMPs[J].J Huazhong Univ Sci Technolog Med Sci,2015,35(1):111-116.
[7] 向敏,赵勇,王先梅.MMP-7在IPF中的水平及意义[J].遵义医学院学报,2012,35(5):400-403.
[8] Achauer B M,Vander Kam V M.Vascular lesions[J].Clin Plast Surg,1993,20(1):43.
[9] 朱晓磊,朱自江,王文昊,等.MMP-7、TIMP-2在食管癌组织的表达及预后关系[J].中国肿瘤外科杂志,2018,10(2):96-100.
[10] 彭莉,李丹.血清IL-17和MMP-7检测在肝细胞癌诊断中的作用[J].中国医科大学学报,2017,46(3):248-250.
[11]章冬银,孙冰丽,顾月清.基质金属蛋白酶7在肿瘤中的作用及其机制研究进展[J].药物生物技术,2011,18(5):457-460.
[12]王婕,曲义坤,国麒麟,等.联合检测血清 MMP -7及CA19 -9对鉴别胰腺癌与慢性胰腺炎的意义[J].黑龙江医药科学,2017,40(2):87-89.
[13]Almendro V,Ametuer E,Garcia-Recio S.The role of MMP-7 and its cross-talk with the FAs/FAsL system during the acquisition of chemoresistance to oxaliplatin[J].Plos One,2009,4(3):e4728.
[14]Huo N,IchikawaY,Kamiyama M,et al.MMP-7(matrilys-in) accelerated growth of human umbilical vein endothelial cells[J].Cancer Lett,2002,177(1):95-100.
[15]Hofmann U B,Westphal J R,VanMuijen G N,et al.Matrixmetalloproteinases in human melanoma[J].J Invest Dermatol,2000,115(3):337-344.
[16]Zhong S,Yang G,Xia C,et al.Expression of matrix metalloproteinase and its tissue inhibitor inhaemangioma[J].J Hua zhong Univ Sci Technol Med Sci,2009,29(5):614-619.
[17]Annabi B,Lachambre M ,Plouffe K,et al.Propranolol adrenergic blockade inhibits human brain enothelial cells tubulogenesis and matrix metalloproteinase-9 secretion[J].Pharmacol Res,2009,60(5):438-445.
[18]Thaker P H,Han L Y,Kamat A A,et al.Chronic stress promotes tumor growth and angiogenesis in a mouse model of ovariancarcinoma[J].Nat Med,2006,12 (8):939-944.
[19]Yang E V,Sood A K,Chen M,et al.Norepinephrime up-regulates the expression of vascular endotheilal growth factor,matrix metalloproteinase(MMP-2),and MMP-9 in nasopharvngeal carcinoma tumorcells[J].Cancer Res,2006,66(21):10357-10364.
[20]Annabi B,Lachambre M P,Plouffe K,et al.Propranolol adrenergic blockade inhibits human brain enothelial cells tubulogenesis and matrix metalloproteinase-9 secretion[J].Pharmacol Res,2009,60(5):438-445.
[21]Sood A K,Bhatty R K,Amat A A,et al.Stress hormone-mediated invasion of ovarian cancer cells[J].Clin Cancer Res,2006,12(2):369-375.
[22]袁蔚力,王绪凯.普萘洛尔治疗血管瘤分子生物学机制及其临床应用研究[J].中国实用口腔科杂志,2012,5(2):116-119.
[23]Jadhav U,Chigurupati S,Lakka S S,et al.Inhibition of matrix metalloproteinase-9 reduces in vitro invasion and angiogenesis in human microvascular endothelial cells[J].Int J Oncol,2004,25(5):1407-1414.
[24]Yin H,Sheng Z,Zhang X,et al.Overexpression of SOX18 promotes prostate cancer progression via the regulation of TCF1,cMyc,cyclin D1 and MMP-7[J].Oncol Rep,2017,37(2):1045-1051.
[25]Pan W K,Li P,Guo Z T,et al.Propranolol induces regression of Hemangima cells via the down regulation of the PI3K/Akt/eNOS/VEGF pathway[J].Pediatricblood & Cancer,2015,62(8):1414-1420.
[2] Sadlecki P,Bodnar M,Grabiec M,et al,The role of Hypoxia-Inducible factor-1α glucose transporter-1,(GLUT-1)and carbon anhydrase IX in endometrial cancer patient[J].Biomed Res Int,2014,2014:616850.
[3] Vosseler S,Lederle W,Airola K,et al.Distinct progression-associated expression of tumor and stromal MMPs in HaCaT skin SCCs correlates with onset of invasion[J].Int J Cancer,2009,125(10):2296-2306.
[4] Wu T,Li Y,Lu J,et al.Increased MMP-21 expression is associated with poor overall survival of patients with gastric cancer[J].Med Oncol,2013,30(1):323.
[5] Shi H,Liu L,Liu L M,et al.Inhibition of tumor growth by β-elemene through downregulation of the expression of uPA,uPAR,MMP-2,and MMP-9 in a murine intraocular melanoma model[J].Melanoma Res,2015,25(1):15-21.
[6] Shi C H,Wang W S,Zhang Z D,et al.Effect of lentivirus-mediated uPA silencing on the proliferation and apoptosis of chondrocytes and the expression of MMPs[J].J Huazhong Univ Sci Technolog Med Sci,2015,35(1):111-116.
[7] 向敏,赵勇,王先梅.MMP-7在IPF中的水平及意义[J].遵义医学院学报,2012,35(5):400-403.
[8] Achauer B M,Vander Kam V M.Vascular lesions[J].Clin Plast Surg,1993,20(1):43.
[9] 朱晓磊,朱自江,王文昊,等.MMP-7、TIMP-2在食管癌组织的表达及预后关系[J].中国肿瘤外科杂志,2018,10(2):96-100.
[10] 彭莉,李丹.血清IL-17和MMP-7检测在肝细胞癌诊断中的作用[J].中国医科大学学报,2017,46(3):248-250.
[11]章冬银,孙冰丽,顾月清.基质金属蛋白酶7在肿瘤中的作用及其机制研究进展[J].药物生物技术,2011,18(5):457-460.
[12]王婕,曲义坤,国麒麟,等.联合检测血清 MMP -7及CA19 -9对鉴别胰腺癌与慢性胰腺炎的意义[J].黑龙江医药科学,2017,40(2):87-89.
[13]Almendro V,Ametuer E,Garcia-Recio S.The role of MMP-7 and its cross-talk with the FAs/FAsL system during the acquisition of chemoresistance to oxaliplatin[J].Plos One,2009,4(3):e4728.
[14]Huo N,IchikawaY,Kamiyama M,et al.MMP-7(matrilys-in) accelerated growth of human umbilical vein endothelial cells[J].Cancer Lett,2002,177(1):95-100.
[15]Hofmann U B,Westphal J R,VanMuijen G N,et al.Matrixmetalloproteinases in human melanoma[J].J Invest Dermatol,2000,115(3):337-344.
[16]Zhong S,Yang G,Xia C,et al.Expression of matrix metalloproteinase and its tissue inhibitor inhaemangioma[J].J Hua zhong Univ Sci Technol Med Sci,2009,29(5):614-619.
[17]Annabi B,Lachambre M ,Plouffe K,et al.Propranolol adrenergic blockade inhibits human brain enothelial cells tubulogenesis and matrix metalloproteinase-9 secretion[J].Pharmacol Res,2009,60(5):438-445.
[18]Thaker P H,Han L Y,Kamat A A,et al.Chronic stress promotes tumor growth and angiogenesis in a mouse model of ovariancarcinoma[J].Nat Med,2006,12 (8):939-944.
[19]Yang E V,Sood A K,Chen M,et al.Norepinephrime up-regulates the expression of vascular endotheilal growth factor,matrix metalloproteinase(MMP-2),and MMP-9 in nasopharvngeal carcinoma tumorcells[J].Cancer Res,2006,66(21):10357-10364.
[20]Annabi B,Lachambre M P,Plouffe K,et al.Propranolol adrenergic blockade inhibits human brain enothelial cells tubulogenesis and matrix metalloproteinase-9 secretion[J].Pharmacol Res,2009,60(5):438-445.
[21]Sood A K,Bhatty R K,Amat A A,et al.Stress hormone-mediated invasion of ovarian cancer cells[J].Clin Cancer Res,2006,12(2):369-375.
[22]袁蔚力,王绪凯.普萘洛尔治疗血管瘤分子生物学机制及其临床应用研究[J].中国实用口腔科杂志,2012,5(2):116-119.
[23]Jadhav U,Chigurupati S,Lakka S S,et al.Inhibition of matrix metalloproteinase-9 reduces in vitro invasion and angiogenesis in human microvascular endothelial cells[J].Int J Oncol,2004,25(5):1407-1414.
[24]Yin H,Sheng Z,Zhang X,et al.Overexpression of SOX18 promotes prostate cancer progression via the regulation of TCF1,cMyc,cyclin D1 and MMP-7[J].Oncol Rep,2017,37(2):1045-1051.
[25]Pan W K,Li P,Guo Z T,et al.Propranolol induces regression of Hemangima cells via the down regulation of the PI3K/Akt/eNOS/VEGF pathway[J].Pediatricblood & Cancer,2015,62(8):1414-1420.