摘要
目的研究基质金属蛋白酶-7(MMP-7)在增生期、消退期婴幼儿血管瘤及正常皮肤组织中表达情况,并探讨其临床意义。方法采用即用型快捷免疫组化MaxVision/HRP检测法检测MMP-7、Ki67在增生期血管瘤(20例)、消退期血管瘤(20例)及正常皮肤(20例)表达情况;夹心法酶联免疫吸附法(ELISA)检测增生期血管瘤患者血清(36例)与正常对照组(36例)血清中MMP-7的表达情况。结果 MMP-7在增生期婴幼儿血管瘤中高表达,在消退期弱阳性表达,在正常皮肤中不表达。增生期与消退期、增生期与对照组比较差异均有统计学意义(P<0.05);Ki67在增生期高表达,消退期低表达,在正常组织中不表达或弱表达,增生期与消退期、增生期与正常组织比较差异有统计学意义(P<0.05);MMP-7在血管瘤患儿血清中的表达水平较正常对照组高,两者比较差异有统计学意义(P<0.05)。结论 MMP-7在血管瘤中有阳性表达,其不同表达水平可能和血管瘤发生发展消退有关。
Objective To study the expression of matrix metalloproteinase-7(MMP-7) in infantile hemangioma and normal skin tissue in the proliferative and regressive stages,and further explore its clinical significance.Methods MaxVision/HRP was used to detect the expression of MMP-7 and Ki67 in proliferative hemangioma(20 cases),regression hemangioma(20 cases) and normal skin(20 cases).The expression of MMP-7 in serum of 36 patients with hemangioma at the proliferative stage and the control group(36 patients) was detected by Sandwich ELISA.Results MMP-7 was highly expressed in infantile hemangioma in the proliferative phase and weakly positive in the regression phase,while no expression was shown in normal skin.There were statistically significant differences between the hyperplasia phase and the regression phase and between the hyperplasia phase and the control group.Ki67 was highly expressed in the proliferative phase,but was poorly expressed in the regression phase,and was not expressed in normal tissues.The difference of Ki67 expression between the proliferative phase and the regression phase,and between the proliferative phase and normal tissues was statistically significant.The expression of MMP-7 in serum of children with hemangioma was higher than that in the normal control group.Conclusion MMP-7 is positively expressed in hemangioma,and its different expression might be related to the occurrence,development and regression of hemangioma.
引文
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