红景天苷对慢性肾衰竭大鼠保护作用机制的研究
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摘要
目的观察红景天苷治疗慢性肾衰竭大鼠肾功能减退的效果,并探讨其可能的机制。方法将实验大鼠随机分为模型组、红景天苷组及假手术组。模型组和红景天苷组大鼠采用切除5/6肾脏法建造慢性肾衰竭大鼠模型。红景天苷组给予红景天苷水溶液灌胃,模型组及假手术组给予蒸馏水灌胃。造模完成后通过HE染色法观察肾组织病理变化,全自动生化仪测定血清肌酐(serum creatinine,SCr)、尿素氮(blood urea nitrogen,BUN)水平以了解肾脏损伤的程度,试剂盒检测肾组织中丙二醛(superoxide dismutase,SOD)、超氧化物歧化酶(malondialdehyde,MDA)水平。结果模型组血浆SCr和BUN水平显著高于假手术组,肾组织SOD水平显著低于假手术组,MDA水平显著高于假手术组(P<0.05);红景天苷组血浆SCr和BUN水平显著低于模型组,但仍高于假手术组,红景天苷组肾组织SOD水平高于模型组,但仍低于假手术组,红景天苷组肾组织MDA水平显著低于模型组,但仍高于假手术组(P<0.05)。结论红景天苷可以有效缓解慢性肾衰竭大鼠的氧化应激水平,对肾脏有保护作用,可能是通过抑制抗氧化酶活性实现的。
Objective To investigate the effect of the Chinese herb Salidroside on chronic renal failure in rats, and to explore its possible mechanism. Methods Experimental animals were randomly assigned as chronic renal failure(CRF) group with 5/6 nephrectomy, Salidroside treatment group and sham-operated control group. Animals in the former two groups were subjected to 5/6 kidney resection to construct CRF rat models. Salidroside group received Salidroside for 4 weeks. The other two groups were given distilled water. At the end of the 4 weeks, kidney tissues were assessed by histopathologic analyses. The levels of serum creatinine(SCr) and blood urea nitrogen(BUN) were measured by automatic biochemical analyzer to estimate the renal function. Malondialdehyde(MDA) and superoxide dismutase(SOD) levels in the kidney tissues were measured by the kit. Results Compared with control group, the levels of serum BUN, SCr and MDA in the other two groups were significantly increased, whereas the level of SOD was declined(P<0.05). Compared with CRF group, the levels of serum BUN, SCr and MDA in the Salidroside group were obviously lower, whereas the level of SOD was higher(P<0.05). Conclusion Salidroside supplementation can effectively alleviate the oxidative stress level and provide protective effect in CRF rats, which may be achieved by the suppression of the antioxidant enzyme activities.
Objective To investigate the effect of the Chinese herb Salidroside on chronic renal failure in rats, and to explore its possible mechanism. Methods Experimental animals were randomly assigned as chronic renal failure(CRF) group with 5/6 nephrectomy, Salidroside treatment group and sham-operated control group. Animals in the former two groups were subjected to 5/6 kidney resection to construct CRF rat models. Salidroside group received Salidroside for 4 weeks. The other two groups were given distilled water. At the end of the 4 weeks, kidney tissues were assessed by histopathologic analyses. The levels of serum creatinine(SCr) and blood urea nitrogen(BUN) were measured by automatic biochemical analyzer to estimate the renal function. Malondialdehyde(MDA) and superoxide dismutase(SOD) levels in the kidney tissues were measured by the kit. Results Compared with control group, the levels of serum BUN, SCr and MDA in the other two groups were significantly increased, whereas the level of SOD was declined(P<0.05). Compared with CRF group, the levels of serum BUN, SCr and MDA in the Salidroside group were obviously lower, whereas the level of SOD was higher(P<0.05). Conclusion Salidroside supplementation can effectively alleviate the oxidative stress level and provide protective effect in CRF rats, which may be achieved by the suppression of the antioxidant enzyme activities.
引文
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[8] Kim S,Yang JY,Jung ES,et al.Effects of sodium citrate on salt sensitivity and kidney injury in chronic renal failure[J].J Korean Med Sci,2014,29(12):1658-1664.
[9] Askari H,Seifi B,Kadkhodaee M,et al.Protective effects of hydrogen sulfide on chronic kidney disease by reducing oxidative stress,inflammation and apoptosis[J].EXCLI J,2018,17:14-23.
[10] 韩雪娇,郭娜,朱美宣,等.红景天苷药理作用及其作用机理研究进展[J].中国生化药学杂志,2015,35(1):171-175.
[11] 龚淼,张雷,赵春芳,等.普罗布考对小鼠肾缺血再灌注损伤的保护作用与机制[J].河北医科大学学报,2017,38(5):497-500,505.
[12] Zhang Q,Liu X,Li N,et al.Sirtuin 3 deficiency aggravates contrast-induced acute kidney injury[J].J Transl Med,2018,16(1):313.
[13] Zheng M,Ye S,Chen Y,et al.A study of urinary Tamm-Horsfall protein excretion in adult type 2 diabetes mellitus[J].Clin Nephrol,2018,90(1):40-45.
[14] Al-Biltagi M,Tolba A,Ezhafez MA,et al.Oxidative stress and cardiac dysfunction in children with chronic renal failure on regular hemodialysis[J].Pediatr Nephrol,2016,31(8):1329-1339.
[15] Weihong C,Bin C,Jianfeng Y.Transmembrane protein 126B protects against high fat diet(HFD)-induced renal injury by suppressing dyslipidemia via inhibition of ROS[J].Biochem Biophys Res Commun,2019,509(1):40-47.
[16] Chillon JM,Massy ZA,Stengel B.Neurological complications in chronic kidney disease patients[J].Nephrol Dial Transplant,2016,31(10):1606-1614.
[17] Chang XY,Cui L,Wang XZ,et al.Quercetion attenuates vascular calcification through suppressed oxidative stress in adenine-induced chronic renal failure rats[J].Biomed Res Int,2017,2017:5716204.
[18] Nuhu F,Bhandari S.Oxidative stress and cardiovascular complications in chronic kidney disease,the impact of anaemia[J].Pharmaceuticals(Basel),2018,11(4).pii:E103.
[19] 侯丹,许光远,张茁,等.红景天苷改善糖尿病大鼠肝脏糖脂水平的作用机制[J].中国实验方剂学杂志,2019,25(1):130-134.
[20] 宋向军.红景天苷对链脲霉素诱导糖尿病大鼠脑组织氧化应激的影响[J].国际中医中药杂志,2015,37(9):812-816.
[21] 金艳玲,周洋,王洪海,等.红景天苷对大鼠肾缺血再灌注损伤的预防与保护作用[J].中药新药与临床药理,2010,21(1):22-25.
[22] 刘娜,徐明,王华龙,等.红景天对血管性痴呆大鼠认知功能的影响[J].河北医科大学学报,2017,38(6):621-624,632.
[2] D′Apolito M,Du X,Pisanelli D,et al.Urea-induced ROS caise endothelial dysfunction in chronic renal failure[J].Atherosclerosis,2015,239(2):393-400.
[3] Li Y,Wu J,Shi R,et al.Antioxidative effects of rhodiola genus:phytochemistry and pharmacological mechanisms against the diseases [J].Curr Top Med Chem,2017,17(15):1692-1708.
[4] Wei Y,Hong H,Zhang X,et al.Salidroside inhibits inflammation through PI3K/Akt/HIF signaling after focal cerebral ischemia rats[J].Inflammation,2017,40(4):1297-1307.
[5] 周林甫,石亚军,焦亚楠,等.红景天苷对大鼠脑缺血再灌注氧化应激损伤的保护作用[J].神经解剖学杂志,2018,34(5):561-566.
[6] Vaziri ND,Dicus M,Ho ND,et al.Oxidative stress and dysregulation of superoxide dismutase and NADPH oxidase in renal insufficiency[J].Kidney Int,2003,63(1):179-185.
[7] Eyre HJ,Speight T,Glazier JD,et al.UrotensinⅡin the development and progression of chronic kidney disease following 5/6 nephrectomy in the rat[J].Exp Physiol,2019,104(3):421-433.
[8] Kim S,Yang JY,Jung ES,et al.Effects of sodium citrate on salt sensitivity and kidney injury in chronic renal failure[J].J Korean Med Sci,2014,29(12):1658-1664.
[9] Askari H,Seifi B,Kadkhodaee M,et al.Protective effects of hydrogen sulfide on chronic kidney disease by reducing oxidative stress,inflammation and apoptosis[J].EXCLI J,2018,17:14-23.
[10] 韩雪娇,郭娜,朱美宣,等.红景天苷药理作用及其作用机理研究进展[J].中国生化药学杂志,2015,35(1):171-175.
[11] 龚淼,张雷,赵春芳,等.普罗布考对小鼠肾缺血再灌注损伤的保护作用与机制[J].河北医科大学学报,2017,38(5):497-500,505.
[12] Zhang Q,Liu X,Li N,et al.Sirtuin 3 deficiency aggravates contrast-induced acute kidney injury[J].J Transl Med,2018,16(1):313.
[13] Zheng M,Ye S,Chen Y,et al.A study of urinary Tamm-Horsfall protein excretion in adult type 2 diabetes mellitus[J].Clin Nephrol,2018,90(1):40-45.
[14] Al-Biltagi M,Tolba A,Ezhafez MA,et al.Oxidative stress and cardiac dysfunction in children with chronic renal failure on regular hemodialysis[J].Pediatr Nephrol,2016,31(8):1329-1339.
[15] Weihong C,Bin C,Jianfeng Y.Transmembrane protein 126B protects against high fat diet(HFD)-induced renal injury by suppressing dyslipidemia via inhibition of ROS[J].Biochem Biophys Res Commun,2019,509(1):40-47.
[16] Chillon JM,Massy ZA,Stengel B.Neurological complications in chronic kidney disease patients[J].Nephrol Dial Transplant,2016,31(10):1606-1614.
[17] Chang XY,Cui L,Wang XZ,et al.Quercetion attenuates vascular calcification through suppressed oxidative stress in adenine-induced chronic renal failure rats[J].Biomed Res Int,2017,2017:5716204.
[18] Nuhu F,Bhandari S.Oxidative stress and cardiovascular complications in chronic kidney disease,the impact of anaemia[J].Pharmaceuticals(Basel),2018,11(4).pii:E103.
[19] 侯丹,许光远,张茁,等.红景天苷改善糖尿病大鼠肝脏糖脂水平的作用机制[J].中国实验方剂学杂志,2019,25(1):130-134.
[20] 宋向军.红景天苷对链脲霉素诱导糖尿病大鼠脑组织氧化应激的影响[J].国际中医中药杂志,2015,37(9):812-816.
[21] 金艳玲,周洋,王洪海,等.红景天苷对大鼠肾缺血再灌注损伤的预防与保护作用[J].中药新药与临床药理,2010,21(1):22-25.
[22] 刘娜,徐明,王华龙,等.红景天对血管性痴呆大鼠认知功能的影响[J].河北医科大学学报,2017,38(6):621-624,632.