左旋芳樟醇及其两种复方精油的催眠镇静作用
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摘要
探讨左旋芳樟醇及其两种复方精油(1号复方精油配方:左旋芳樟醇∶薰衣草精油∶依兰依兰油∶柏木油=1∶2∶4∶3,2号复方精油配方:左旋芳樟醇∶薰衣草精油∶依兰依兰油∶柏木油=5∶3∶1∶1)对小鼠的催眠镇静作用,研究精油对小鼠自主活动、阈上剂量戊巴比妥钠致小鼠睡眠潜伏期和睡眠时间、阈下剂量戊巴比妥钠致小鼠睡眠只数的影响.结果表明:3种精油对小鼠均有明显的催眠镇静作用,其中以浓度为10-3的2号复方精油的效果最佳;在小鼠自主活动能力测试中,2号复方精油组小鼠的总路程与对照组的差异极显著(P<0.01),休息时间、活动时间、活跃度、中间区域时间和中央区域路程与对照组的差异显著(P<0.05);与对照组相比较,2号复方精油极显著缩短小鼠睡眠潜伏期(P<0.01),从(18.47±2.04)s缩短到(14.13±1.73)s,极显著延长小鼠睡眠时间(P<0.01),从(27.01±5.84)s延长到(40.41±8.36)s,增加小鼠睡眠只数(P<0.01),从1只增加到7只.可见,左旋芳樟醇及其两种复方精油具有明显的催眠镇静作用,以2号复方精油的效果最佳.
This paper studied the hypnotic and sedative effect of three kinds of aromatic essential oil,L-linalool and two kinds of blended oil which contained L-linalool( No. 1 essential oil,L-linalool∶ lavender essential oil∶ Yilang Yilang oil∶ cedar oil = 1∶ 2∶ 4∶ 3; No. 2 essential oil,L-linalool∶ lavender essential oil∶ Yilang Yilang oil∶ cedar oil = 5∶ 3∶ 1∶ 1). Spontaneous locomotor activity,sleep latency and time were monitored with sleeping induced by pentobarbital sodium. Spontaneous locomotor activity results showed that all the five treated groups walked significantly shorter distance than that of CK group( P < 0. 01). All the five groups varied from the CK group in the frequency of standing,resting duration,active time and central region distance( P < 0. 05).No. 2 blended oil in the concentration of 10- 3led to the largest number of sleepy mice compared with other groups including CK group( P < 0. 01). Mice treated with No. 2 blended oil in the concentration of 10- 3had the shortest sleep latency and longest sleep duration( P < 0. 01). In conclusion,L-linalool and two kinds of blended oil containing L-linalool performed notable hypnotic and sedative function,with No. 2 blended oil presenting the best efficacy.
This paper studied the hypnotic and sedative effect of three kinds of aromatic essential oil,L-linalool and two kinds of blended oil which contained L-linalool( No. 1 essential oil,L-linalool∶ lavender essential oil∶ Yilang Yilang oil∶ cedar oil = 1∶ 2∶ 4∶ 3; No. 2 essential oil,L-linalool∶ lavender essential oil∶ Yilang Yilang oil∶ cedar oil = 5∶ 3∶ 1∶ 1). Spontaneous locomotor activity,sleep latency and time were monitored with sleeping induced by pentobarbital sodium. Spontaneous locomotor activity results showed that all the five treated groups walked significantly shorter distance than that of CK group( P < 0. 01). All the five groups varied from the CK group in the frequency of standing,resting duration,active time and central region distance( P < 0. 05).No. 2 blended oil in the concentration of 10- 3led to the largest number of sleepy mice compared with other groups including CK group( P < 0. 01). Mice treated with No. 2 blended oil in the concentration of 10- 3had the shortest sleep latency and longest sleep duration( P < 0. 01). In conclusion,L-linalool and two kinds of blended oil containing L-linalool performed notable hypnotic and sedative function,with No. 2 blended oil presenting the best efficacy.
引文
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[15]GULCIN I,SAT I G,BEYDEMIR S,et al.Comparison of antioxidant activity of clove buds and lavender[J].Food Chem,2004,87(3):393-400.
[16]SHEN J,NIIJIMA A,TANIDA M,et al.Olfactory stimulation with scent of lavender oil affects autonomic nerves,lipolysis and appetite in rats[J].Neurosci Lett,2005,383(1-2):188-193.
[2]林翔云.调香术[M].2版.北京:化学工业出版社,2008:159-160.
[3]LINCK V M,SILVA A L,FIGUEIRO M,et al.Inhaled linalool-induced sedation in mice[J].Phyto Medicine,2009,16(4):303-307.
[4]UMEZU T,NAGANO K,ITO H,et al.Antieonflict effects of lavender oil and identification of its active constituents[J].Pharrnacol Biochem Behav,2006,85(4):713-721.
[5]王善懿.一种复方香精油的镇静催眠功效及应用研究[D].上海:上海交通大学,2010.
[6]SUGAWARA Y,HARA C,TAMURA K,et al.Sedative effect on humans of inhalation of essential oil of linalool:Sensory evaluation and physiological measurements using optically active linalools[J].Anal Chim Acta,1998,365(1/3):293-299.
[7]刘静,徐江涛.芳香疗法镇静催眠作用的研究进展[J].医学综述,2012,18(5):756-758.
[8]翟秀丽,俞益武,吴媛媛,等.芳香疗法研究进展[J].香精香料化妆品,2011(6):45-50.
[9]杨海清,李忠海,付湘晋,等.以左旋芳樟醇为主要原料配制复方精油的研究[J].中南林业科技大学学报,2013,33(3):112-114,118.
[10]陈奇.中药药理研究方法学[M].2版.北京:人民卫生出版社,2006:805.
[11]买文丽,王琼,刘新民,等.小鼠自主活动实验中的评价指标[J].中国实验动物学报,2008,16(3):173-175.
[12]WITT R,GALLIGAN M,DESPINOY J,et al.Olfactory behavioral testing in the adult mouse[J].Journal of Visualized Experiments,2009,23:949.
[13]赵江丽,吴向阳,仰榴青,等.夏枯草镇静与催眠作用的初步研究[J].时珍国医国药,2009,20(2):443-444.
[14]PAREJO I,VILADOMAT F,BASTIDA J,et al.Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled Mediterranean herbs and aromatic plants[J].Journal Agric Food Chem,2002,50:6882-6890.
[15]GULCIN I,SAT I G,BEYDEMIR S,et al.Comparison of antioxidant activity of clove buds and lavender[J].Food Chem,2004,87(3):393-400.
[16]SHEN J,NIIJIMA A,TANIDA M,et al.Olfactory stimulation with scent of lavender oil affects autonomic nerves,lipolysis and appetite in rats[J].Neurosci Lett,2005,383(1-2):188-193.