污水中耐红霉素细菌的复活特性研究
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摘要
耐红霉素(药物)细菌和基因在污水处理厂污水中普遍被检出。研究了耐红霉素细菌在不同消毒剂量、复活条件、复活强度条件下的复活程度,并对紫外线消毒处理后红霉素耐药性的后续生态风险进行了评价。结果表明:在紫外线消毒剂量为1mJ/cm~2时,耐红霉素细菌的复活程度比10mJ/cm~2时更为显著;对于耐红霉素细菌的复活,光复活处理比暗复活处理更为显著;对于耐红霉素细菌的暗复活,长时间的暗复活处理会增加耐红霉素细菌的复活程度,提升其生态风险。因此须重视耐药细菌复活在污水中的生态风险。
Erythromycin resistance bacteria and genes are generally detected in wastewater treatment plants.This paper investigates the reactivation characteristic of erythromycin resistance bacteria under a variety of disinfection dosage,resuscitative mode and reactivation intensity,and evaluates the subsequent ecological risk of erythromycin resistance after UV disinfection.The results show that the reactivation of erythromycin resistant bacteria is significantly higher at disinfection dosage of 10 mJ/cm~2 than that of 1 mJ/cm~2.The light reactivation is more remarkable than the dark reactivation;and for erythromycin resistance bacteria,long time dark reactivation treatment will increase the reactivation degree and raise the ecological risk.Thus,the paper indicates that there is an urgent need to focus on the ecological risk of the reactivation of drug-resistant bacteria in municipal wastewater treatment plants.
Erythromycin resistance bacteria and genes are generally detected in wastewater treatment plants.This paper investigates the reactivation characteristic of erythromycin resistance bacteria under a variety of disinfection dosage,resuscitative mode and reactivation intensity,and evaluates the subsequent ecological risk of erythromycin resistance after UV disinfection.The results show that the reactivation of erythromycin resistant bacteria is significantly higher at disinfection dosage of 10 mJ/cm~2 than that of 1 mJ/cm~2.The light reactivation is more remarkable than the dark reactivation;and for erythromycin resistance bacteria,long time dark reactivation treatment will increase the reactivation degree and raise the ecological risk.Thus,the paper indicates that there is an urgent need to focus on the ecological risk of the reactivation of drug-resistant bacteria in municipal wastewater treatment plants.
引文
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[2]沈怡雯,黄智婷,谢冰.抗生素及其抗性基因在环境中的污染、降解和去除研究进展[J].应用与环境生物学报,2015,21(2):181-187.
[3]DCosta V M,McGrann K M,Hughes D W,et al.Sampling the antibiotic resistome[J].Science,2006,311:374-377.
[4]Gao P,Mao D,Luo Y,et al.Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment[J].Water Research,2012,46(7):2355-2364.
[5]Pruden A,Pei R,Storteboom H.Antibiotic resistance genes as emerging contaminants:Studies in Northern Colorado[J].Environmental Science and Technology,2006,40(23):7445-7450.
[6]WHO.Antimicrobial Resistance:Global Report on Surveillance[R].Geneva,Switzerland:WHO,2014.
[7]Reinthaler F F,Posch J,Feierl G,et al.Antibiotic resistance of E.coli in sewage and sludge[J].Water Research,2003,37(8):1685-1690.
[8]McKinney C W,Pruden A.Ultraviolet disinfection of antibiotic resistant bacteria and their antibiotic resistance genes in water and wastewater[J].Environmental Science and Technology,2012,46(24):13393-13400.
[9]Macauley J J,Qiang Z,Adams C D,et al.Disinfection of swine wastewater using chlorine,ultraviolet light and ozone[J].Water Research,2006,40(10):2017-2026.
[10]张崇淼,刘强强,国承荣.紫外线消毒后耐药性大肠埃希氏菌的光复活特性[J].环境工程学报,2017,11(4):2145-2149.
[11]Templeton M R,Leung O F,Rogers W M.Chlorine and UV disinfection of ampicillin-resistant and trimethoprim-resistant Escherichia coli[J].Canadian Journal of Civil Engineering,2009,36:889-894.
[12]Auerbach E A,Seyfried E E,McMahon K D.Tetracycline resistance genes in activated sludge wastewater treatment plants[J].Water Reserch,2007,41(5):1143-1151.
[13]Bolton J R,Linden K G.Standardization of methods for fluence(UV dose)determination in bench-scale UV experiments[J].Journal of Environmental Engineering,2003,129(3):209-215.
[14]CLSI.Performance Standards for Antimicrobial Susceptibility Testing:Twenty-first Informational Supplement CLSI Document M100-S21[M].Wayne,PA,USA:Clinical and Laboratory Standards Institute,2011.
[15]Nebot Sanz E,Salcedo Davila I,Andrade Balao J A,et al.Modelling of reactivation after UV disinfection:Effect of UV-C dose on subsequent photoreactivation and dark repair[J].Water Research,2007,41(14):3141-3151.
[16]Munir M,Wong K,Xagoraraki I.Release of antibiotic resistant bacteria and genes in the effluent and biosolids of five wastewater utilities in Michigan[J].Water Research,2011,45(2):681-693.