不同LED光源对蝴蝶兰增殖和壮苗培养的影响
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摘要
为研究不同发光二极管(LED)对增殖及壮苗培养的影响,从而为优化其组培条件提供基础。以蝴蝶兰3个不同的栽培品种为材料,采用不同LED光源照射,结果显示:对‘大辣椒’以连续性照射的T5-红蓝光或T8-红蓝光为增殖阶段的适宜光源,或以间歇性照射的T5-红蓝光或T8-红蓝光为增殖阶段的适宜光源;对‘柳林黑玫瑰’以连续性照射的T8-红蓝光为增殖阶段的适宜光源,或以间歇性照射的T5-红蓝光或T8-红蓝光为增殖阶段的适宜光源;对‘文景天使’以连续性照射的T5-红蓝光为增殖阶段的最适光源。在壮苗培养阶段,对‘大辣椒’以连续性照射的T5-红蓝光或T8-红蓝光为最适光源,对‘柳林黑玫瑰’和‘文景天使’以连续性照射的T5-红蓝光为最适光源。以上结果说明,红蓝复色光更有利于蝴蝶兰的单芽增殖以及根系诱导,是具有优势的植物组织培养光源。
In order to study effects of different light-emitting diodes on multiplication and strengthening cultivation of Phalaenopsis ssp.seedlings,and to provide some basises for optimization of culture conditions,we used 3 cultivars of Phalaenopsis ssp.as materials to investigate effect of different LED illuminans on multiplication and strengthening cultivation in vitro.The results show that at multiplication stage,continuous irradiation or intermittent irradiation of T5-red and blue or T8-red and blue was the suitable illuminant for ‘Big Chilli',and continuous irradiation of T5-red and blue was the optimal illuminant for ‘Vimtech Angel'.At strengthening cultivation stage,continuous irradiation of T5-red and blue or T8-red and blue was the optimal illuminant for ‘Big Chilli',and continuous irradiation of T5-red and blue was the optimal illuminant for ‘Willow Black Rose' and ‘Vimtech Angel'.It was concluded that the combination of red and blue LED lights benefited single bud multiplication and root system induction in Phalaenopsis ssp.,and it could be the light source for plant tissue culture with advantages.
In order to study effects of different light-emitting diodes on multiplication and strengthening cultivation of Phalaenopsis ssp.seedlings,and to provide some basises for optimization of culture conditions,we used 3 cultivars of Phalaenopsis ssp.as materials to investigate effect of different LED illuminans on multiplication and strengthening cultivation in vitro.The results show that at multiplication stage,continuous irradiation or intermittent irradiation of T5-red and blue or T8-red and blue was the suitable illuminant for ‘Big Chilli',and continuous irradiation of T5-red and blue was the optimal illuminant for ‘Vimtech Angel'.At strengthening cultivation stage,continuous irradiation of T5-red and blue or T8-red and blue was the optimal illuminant for ‘Big Chilli',and continuous irradiation of T5-red and blue was the optimal illuminant for ‘Willow Black Rose' and ‘Vimtech Angel'.It was concluded that the combination of red and blue LED lights benefited single bud multiplication and root system induction in Phalaenopsis ssp.,and it could be the light source for plant tissue culture with advantages.
引文
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[15]郑子松,王红,王神云,等.LED光源对结球甘蓝(Brassica oleracea var.capitatal)不定芽再生的影响[J].天津农学院学报,2013,20(2):6-10.
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[17]刘晓青,苏家乐,陈尚平,等.高山杜鹃叶片再生和试管苗生长对不同LED光质的响应特征[J].江苏农业学报,2013,29(6):1451-1455.
[18]陈志,孙庆丽,汪一婷,等.不同光质对甘蔗组培苗的影响[J].农业工程,2012,2(10):51-57.
[19]Hahn E J,Kozai T,Paek K Y.Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets[J].Journal of Plant Biology,2000,43(4):247-250.
[20]Moon H K,Park S Y,Kim Y W,et al.Growth of Tsuru-rindo(Tripterospermum japonicum)cultured in vitro under various sources of light-emitting diode(LED)irradiation[J].Journal of Plant Biology,2006,49(2):174-179.
[21]Nhut D T,Takamura T,Watanabe H,et al.Sugar-free micropropagation of Eucalyptus citriodora using light emitting diode(LED)and film-rockwool culture system[J].Environmental Control in Biology,2002,40(2):147-155.
[22]Nhut D L,Takamura T,Watanabe H,et al.Artificial light source using light-emitting diode(LEDs)in the efficient micropropagation of Spathiphyllum plantlets[J].Acta Horticulturae,2005,692:137-142.
[23]Rueychi J,Wei F.Growth of potato plantlets in vitro is different when provided concurrent versus alternating blue and red light photoperiods[J].Hort Science,2004,39(2):380-382.
[2]周洲,李永丽,安世恒,等.欧美杨108高效组培再生系统[J].中南林业科技大学学报,2016,36(7):1-6.
[3]孙翊,费如桂,殷丽青,等.培养基和蔗糖对非洲菊增殖及其生长特性的影响[J].经济林研究,2017,35(2):183-187.
[4]Kuan-Hung Lin,Meng-Yuan Huang,Wen-Dar Huang,et al.The effects of red,blue,and white light-emitting diodes on the growth,development,and edible quality of hydroponically grown lettuce(Lactucasativa L var capitata)[J].Scientia Horticulturae,2013,150(2):86-91.
[5]张婕,高亦珂,何琦,等.发光二极管(LED)在菊花组织培养中的应用研究[C].中国观赏园艺研究进展,2008:296-300.
[6]邸秀茹,焦学磊,崔瑾,等.新型光源LED辐射的不同光质配比光对菊花组培苗生长的影响[J].植物生理学通讯,2008,44(4):661-664.
[7]魏星,顾清,戴艳娇,等.不同光质对菊花组培苗生长的影响[J].园艺园林科学,2008,24(12):344-349.
[8]陈颖,王政,纪思羽,等.LED光源不同光质比例对红掌试管苗生长的影响[J].江西农业大学学报,2013,35(2):375-380.
[9]覃婕,黄钊.LED组合光源对红掌愈伤组织培养的影响[J].农业与技术,2017,37(4):32.
[10]王亚沉,包崇格,彭少丹,等.光质对碧玉兰组培苗生长及若干生理指标的影响[J].南方农业学报,2013,44(5):740-744.
[11]Kong S S,Hosakatte N M,Jeong W H,et al.The effect of light quality on the growth and development of in vitro cultured Doritaenopsis plants[J].Acta Physiologiae Plantarum,2008,30(3):339-343.
[12]Hsu H C,Chen C C.The effect of light spectrum on the growth characteristics of in vitro cultures of Phalaenopsis[J].Propag Ornam Plants,2010,10:3-8.
[13]戴艳娇,王琼丽,张欢,等.不同光谱的LEDs对蝴蝶兰组培苗生长的影响[J].江苏农业科学,2010,26(5):227-231.
[14]任桂萍,王小菁,朱根发.不同光质的LED对蝴蝶兰组织培养增殖及生根的影响[J].植物学报,2016,51(1):81-88.
[15]郑子松,王红,王神云,等.LED光源对结球甘蓝(Brassica oleracea var.capitatal)不定芽再生的影响[J].天津农学院学报,2013,20(2):6-10.
[16]Lu Y X,Godo T,Fujiwara K,et al.Effect of nitrogen source and wavelength of LED-light on organogenesis from leaf and shoot tip cultures in Lysionotus pauciflorus Maxim[J].Propag Ornam Plants,2013,13(13):174-180.
[17]刘晓青,苏家乐,陈尚平,等.高山杜鹃叶片再生和试管苗生长对不同LED光质的响应特征[J].江苏农业学报,2013,29(6):1451-1455.
[18]陈志,孙庆丽,汪一婷,等.不同光质对甘蔗组培苗的影响[J].农业工程,2012,2(10):51-57.
[19]Hahn E J,Kozai T,Paek K Y.Blue and red light-emitting diodes with or without sucrose and ventilation affect in vitro growth of Rehmannia glutinosa plantlets[J].Journal of Plant Biology,2000,43(4):247-250.
[20]Moon H K,Park S Y,Kim Y W,et al.Growth of Tsuru-rindo(Tripterospermum japonicum)cultured in vitro under various sources of light-emitting diode(LED)irradiation[J].Journal of Plant Biology,2006,49(2):174-179.
[21]Nhut D T,Takamura T,Watanabe H,et al.Sugar-free micropropagation of Eucalyptus citriodora using light emitting diode(LED)and film-rockwool culture system[J].Environmental Control in Biology,2002,40(2):147-155.
[22]Nhut D L,Takamura T,Watanabe H,et al.Artificial light source using light-emitting diode(LEDs)in the efficient micropropagation of Spathiphyllum plantlets[J].Acta Horticulturae,2005,692:137-142.
[23]Rueychi J,Wei F.Growth of potato plantlets in vitro is different when provided concurrent versus alternating blue and red light photoperiods[J].Hort Science,2004,39(2):380-382.