淳安花猪5个功能基因的多态性研究
摘要
为深入挖掘淳安花猪优良种质特性,本研究运用PCR-RFLP方法,对淳安花猪的雌激素受体(ESR)基因、促卵泡激素β亚基(FSHβ)基因、生长激素受体(GH)基因、黑素皮质激素受体4(MC4R)基因、兰尼定受体(RYR1)基因进行分子遗传学检测。结果表明:淳安花猪MC4R、RYR1基因分别只有1种基因型AA、BB;ESR、GH基因各有2种基因型AB和BB。
引文
[1]章学东,祝荣,王初昌,等.淳安花猪产区调研分析[J].杭州农业与科技,2009,(2):17.
[2]楼立峰,章学东,蔡和平,等.淳安花猪亟待保护[J].杭州农业与科技,2006,(5):25-25.
[3]祝荣.淳安花猪种质资源保护利用及特色文化建设[J].中国猪业,2012,7(5):23-24.
[4]李庆海,章学东,范京辉,等.淳安花猪的品质特性介绍[J].杭州农业与科技,2010,(5):48.
[5]曹果清.猪PRLR基因和FSHβ亚基基因多态性研究[D]:山西农业大学,2004.
[6]Short T,Rothschild M,Southwood O.Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines[J].Journal of Animal Science,1997,75(12):3138-3142.
[7]陈克飞,李宁.猪雌激素受体(ESR)基因对产仔数性状的影响[J].遗传学报,2000,27(10):853-857.
[8]Cooke D,Crowe M,Roche J.Gonadotrophin heterogeneity and its role in farm animal reproduction[J].Animal Reproduction Science,1996,41(2):77-99.
[9]谢飞,吴晗,滕尚辉,等.应用4个繁殖性状候选基因对太湖猪群体遗传结构分析[J].畜牧与兽医,2008,40(6):23-26.
[10]鲁绍雄,胡晓湘,连林生,等.撤坝猪ESR和FSH-β基因多态性及其与产仔数的关联分析[J].云南农业大学学报:自然科学版,2009,24(3):389-393.
[11]冯富彦,李新建,吕刚,等.豫南黑猪FSHβ亚基基因多态性研究[J].中国畜牧兽医,2010,(3):145-148.
[12]Yeo G,Farooqi I,Challis B.The role of melanocortin signalling in the control of body weight:evidence from human and murine genetic models[J].Qjm,2000,93(1):7-14.
[13]Sinha PS,Schi9th HB,Tatro JB.Roles of the melanocortin-4 receptor in antipyretic and hyperthermic actions of centrally administeredα-MSH[J].Brain research,2004,1001(1):150-158.
[14]Zhang C-L,Wang Y-H,Chen H.Association between variants in the 5'-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle[J].Molecular biology reports,2009,36(7):1839-1843.
[15]Jokubka R,Maak S,Kerziene S.Association of a melanocortin 4 receptor(MC4R)polymorphism with performance traits in Lithuanian White pigs[J].Journal of Animal breeding and Genetics,2006,123(1):17-22.
[16]陈敏,王爱国,李宁,等.中国地方猪种MC4R基因频率的研究(英文)[C].全国畜禽遗传资源保护与利用学术研讨会论文集,2005.
[17]Etherton TD,Bauman DE.Biology of somatotropin in growth and lactation of domestic animals[J].Physiological reviews,1998,78(3):745-762.
[18]经荣斌,宋成义,陶勇,等.猪GH基因部分突变位点在不同品种中的分布[J].扬州大学学报:农业与生命科学版,2003,24(1):30-33.
[19]刘丑生.甘肃黑猪合成系RYR1基因的PCR-RFLP检测试验[J].甘肃畜牧兽医,2002,32(2):24-25.
[20]Fujii J,Otsu K,Zorzato F.Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia[J].Science,1991,253(5018):448-451.
[21]朱砺,李学伟,帅素蓉.兰尼定受体基因在新荣昌猪中的分布及其对胴体和肉质性状的效应分析[J].中国畜牧杂志,2005,41(4):14-16.
[22]刘文举,何年华,左本武,等.监利猪和大监猪五个基因的多态性研究[J].养猪,2008,(3):25-29.
[2]楼立峰,章学东,蔡和平,等.淳安花猪亟待保护[J].杭州农业与科技,2006,(5):25-25.
[3]祝荣.淳安花猪种质资源保护利用及特色文化建设[J].中国猪业,2012,7(5):23-24.
[4]李庆海,章学东,范京辉,等.淳安花猪的品质特性介绍[J].杭州农业与科技,2010,(5):48.
[5]曹果清.猪PRLR基因和FSHβ亚基基因多态性研究[D]:山西农业大学,2004.
[6]Short T,Rothschild M,Southwood O.Effect of the estrogen receptor locus on reproduction and production traits in four commercial pig lines[J].Journal of Animal Science,1997,75(12):3138-3142.
[7]陈克飞,李宁.猪雌激素受体(ESR)基因对产仔数性状的影响[J].遗传学报,2000,27(10):853-857.
[8]Cooke D,Crowe M,Roche J.Gonadotrophin heterogeneity and its role in farm animal reproduction[J].Animal Reproduction Science,1996,41(2):77-99.
[9]谢飞,吴晗,滕尚辉,等.应用4个繁殖性状候选基因对太湖猪群体遗传结构分析[J].畜牧与兽医,2008,40(6):23-26.
[10]鲁绍雄,胡晓湘,连林生,等.撤坝猪ESR和FSH-β基因多态性及其与产仔数的关联分析[J].云南农业大学学报:自然科学版,2009,24(3):389-393.
[11]冯富彦,李新建,吕刚,等.豫南黑猪FSHβ亚基基因多态性研究[J].中国畜牧兽医,2010,(3):145-148.
[12]Yeo G,Farooqi I,Challis B.The role of melanocortin signalling in the control of body weight:evidence from human and murine genetic models[J].Qjm,2000,93(1):7-14.
[13]Sinha PS,Schi9th HB,Tatro JB.Roles of the melanocortin-4 receptor in antipyretic and hyperthermic actions of centrally administeredα-MSH[J].Brain research,2004,1001(1):150-158.
[14]Zhang C-L,Wang Y-H,Chen H.Association between variants in the 5'-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle[J].Molecular biology reports,2009,36(7):1839-1843.
[15]Jokubka R,Maak S,Kerziene S.Association of a melanocortin 4 receptor(MC4R)polymorphism with performance traits in Lithuanian White pigs[J].Journal of Animal breeding and Genetics,2006,123(1):17-22.
[16]陈敏,王爱国,李宁,等.中国地方猪种MC4R基因频率的研究(英文)[C].全国畜禽遗传资源保护与利用学术研讨会论文集,2005.
[17]Etherton TD,Bauman DE.Biology of somatotropin in growth and lactation of domestic animals[J].Physiological reviews,1998,78(3):745-762.
[18]经荣斌,宋成义,陶勇,等.猪GH基因部分突变位点在不同品种中的分布[J].扬州大学学报:农业与生命科学版,2003,24(1):30-33.
[19]刘丑生.甘肃黑猪合成系RYR1基因的PCR-RFLP检测试验[J].甘肃畜牧兽医,2002,32(2):24-25.
[20]Fujii J,Otsu K,Zorzato F.Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia[J].Science,1991,253(5018):448-451.
[21]朱砺,李学伟,帅素蓉.兰尼定受体基因在新荣昌猪中的分布及其对胴体和肉质性状的效应分析[J].中国畜牧杂志,2005,41(4):14-16.
[22]刘文举,何年华,左本武,等.监利猪和大监猪五个基因的多态性研究[J].养猪,2008,(3):25-29.