姜黄素对岛状皮瓣缺血再灌注损伤的影响
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摘要
目的探讨姜黄素(curcumin)对大鼠岛状皮瓣(skin flap)缺血再灌注损伤(lschemia-reperfusion injury)的保护作用。方法将105只SD大鼠随机平均分成3组(n=35),每组按术后6、10、20、30 h及7 d分为5个亚组(n=7)。对照组(Ⅰ组):不做缺血再灌注处理。缺血再灌注组(Ⅱ组):手术当天至术后7 d,每日腹腔内注射生理盐水0.4 mL/kg。姜黄素治疗组(Ⅲ组):将生理盐水替换为姜黄素注射液50 mg/kg。在以大鼠旋髂深血管皮支(deep circumflex iliac vessels)为蒂的背部岛状皮瓣缺血再灌注损伤模型上,分别测定皮瓣组织中超氧化物歧化酶(superoxide dismutase,SOD)、髓过氧化物酶(myeloperoxidase,MPO)活性,丙二醛(malondialdehyde,MDA)的含量。并对皮瓣进行图像分析,观察皮瓣存活率。结果与Ⅰ组进行比较,Ⅱ组MDA含量及MPO活性明显升高(P<0.01),SOD活性明显降低(P<0.01)。与Ⅱ组进行比较,Ⅲ组MDA含量及MPO活性明显降低(P<0.01),SOD活性表达明显升高(P<0.01)。术后7 d皮瓣存活率为Ⅰ组>Ⅲ组>Ⅱ组(P<0.01)。结论姜黄素具有抗炎、抗氧化、提高清除氧自由基能力的作用,减轻皮瓣缺血再灌注损伤,促进皮瓣存活。
Objective To investigate the protective effect of curcumin against ischemia-reperfusion injury in rat island skin flaps. Methods A total of 105 Sprague-Dawley rats were randomly divided into three groups(n=35 for each group); each group was divided into five subgroups(n=7 for each group) according to the time after operation(6 h, 10 h, 20 h, 30 h, and 7 d). The control group(group Ⅰ) was not subjected to any ischemia-reperfusion treatment except an intraperitoneal injection of normal saline at a dose of 0.4 ml/kg daily from the day of operation to 7 days after operation. The ischemia-reperfusion group(group Ⅱ) was subjected to ischemia-reperfusion treatment in addition to the same postoperative treatment as group Ⅰ. The curcumin treatment group(group Ⅲ) was subjected to ischemia-reperfusion treatment in addition to the same postoperative treatment as group Ⅰ except that normal saline was replaced by curcumin injection(50 mg/kg). The activities of superoxide dismutase(SOD)and myeloperoxidase(MPO) and the content of malondialdehyde(MDA) were measured in a rat model of ischemia-reperfusion injury in the dorsal island flaps pedicled to the deep circumflex iliac vessels. The viability of the skin flaps was evaluated by analyzing the flaps. Results Compared with group Ⅰ, group Ⅱ had significant increases in both the content of MDA and the activity of MPO(P<0.01), but a significant decrease in SOD activity(P<0.01). Compared with group Ⅱ, group Ⅲ had significant decreases inboth the content of MDA and the activity of MPO(P<0.01), but a significant increase in SOD activity(P<0.01). The viability of the skin flaps 7 days after operation was highest in group Ⅰ, followed by group Ⅲ and then group Ⅱ, with significant differences observed between the groups(P<0.01). Conclusion Curcumin has anti-inflammatory and antioxidant effects, as well as the ability to enhance oxygen free radical scavenging, which together alleviate ischemia-reperfusion injury of skin flaps and improve their viability.
Objective To investigate the protective effect of curcumin against ischemia-reperfusion injury in rat island skin flaps. Methods A total of 105 Sprague-Dawley rats were randomly divided into three groups(n=35 for each group); each group was divided into five subgroups(n=7 for each group) according to the time after operation(6 h, 10 h, 20 h, 30 h, and 7 d). The control group(group Ⅰ) was not subjected to any ischemia-reperfusion treatment except an intraperitoneal injection of normal saline at a dose of 0.4 ml/kg daily from the day of operation to 7 days after operation. The ischemia-reperfusion group(group Ⅱ) was subjected to ischemia-reperfusion treatment in addition to the same postoperative treatment as group Ⅰ. The curcumin treatment group(group Ⅲ) was subjected to ischemia-reperfusion treatment in addition to the same postoperative treatment as group Ⅰ except that normal saline was replaced by curcumin injection(50 mg/kg). The activities of superoxide dismutase(SOD)and myeloperoxidase(MPO) and the content of malondialdehyde(MDA) were measured in a rat model of ischemia-reperfusion injury in the dorsal island flaps pedicled to the deep circumflex iliac vessels. The viability of the skin flaps was evaluated by analyzing the flaps. Results Compared with group Ⅰ, group Ⅱ had significant increases in both the content of MDA and the activity of MPO(P<0.01), but a significant decrease in SOD activity(P<0.01). Compared with group Ⅱ, group Ⅲ had significant decreases inboth the content of MDA and the activity of MPO(P<0.01), but a significant increase in SOD activity(P<0.01). The viability of the skin flaps 7 days after operation was highest in group Ⅰ, followed by group Ⅲ and then group Ⅱ, with significant differences observed between the groups(P<0.01). Conclusion Curcumin has anti-inflammatory and antioxidant effects, as well as the ability to enhance oxygen free radical scavenging, which together alleviate ischemia-reperfusion injury of skin flaps and improve their viability.
引文
[1]姜丽,宁可,包怡敏.三七总皂苷抗缺血再灌注损伤机制研究进展[J].中华中医药学刊,2016,34(2):267-270.
[2]张晶晶.利拉鲁肽对大鼠心肌再灌注损伤的作用及其机制[J].职业与健康,2016,32(1):45-47.
[3]王涵东,梁维邦.姜黄素的研究进展[J].江苏医药,2014,40(10):1193-1194.
[4]王彬辉,章文红,张晓芬,等.姜黄素的药理及剂型研究进展[J].中华中医药学刊,2013,31(5):1102-1105.
[5]张博,叶丽红.姜黄素抗癌机制研究进展[J].中医药学报,2013,41(1):121-122.
[6] TAKIKAWA M, SUMI Y, ISHIHARA M, et al. PRP&F/P MPs Improved Survival of Dorsal Paired Pedicle Skin Flaps in Rats[J]. Journal of Surgical Research, 2011, 170(1):189-196.
[7] YANG D, MORRIS S F. An extended dorsal island skin flap with multiple vascular territories in the rat:A new skin flap model[J]. The Journal of surgical research,1999,87(2):164-170.
[8]刘志辉,刘春丽,张伟.血管内皮细胞生长因子皮下注射对大鼠背部皮瓣成活的实验研究[J].口腔颌面外科杂志,2002,12(1):20-23.
[9]林泉.大鼠轴型皮瓣及其放射性损伤的低能量冲击波治疗的实验研究[D].长春:吉林大学,2010.
[10]赵珊,马迎春,刘亚坤,等.姜黄素通过抑制内质网应激和JNK通路过度活化减轻小鼠肺缺血再灌注损伤[J].中国病理生理杂志,2013,29(2):308-313.
[11]尤佳,戴东波,何雯洁,等.姜黄素长循环脂质体的制备及药动学研究[J].中国中药杂志,2014,39(7):1238-1242.
[12]何双艳,李珏宏,李昌平,等.姜黄素对DSS诱导的溃疡性结肠炎小鼠p38MAPK表达的影响[J].实用医学杂志,2014,30(4):539-541.
[13] GUPTA S C, KISMALI G, AGGARWAL B B. Curcumin, a component of turmeric:from farm to pharmacy[J]. Biofactors, 2013,39(1):2-13.
[14] WU J, LI Q, WANG X, et al. Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway[J]. Plos One, 2013, 8(3):e59843.
[15]王灵聪,张微,夏国莲,等.姜黄素对急性肺栓塞大鼠炎症因子的影响[J].中医杂志,2013,54(6):506-508.
[16]廖端芳,张彩平,顾洪丰,等.烟酸姜黄素脂通过调节细胞自噬和LDLR促进胆固醇逆向转运[C]//杭州:2016心血管药理专业委员会全国心脑血管药理学术会议,2016.
[17]孙四玉,杨冬梅,戴娜,等.乙酰水杨酸姜黄素酯对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖的影响及相关机制[J].湖南中医药大学学报,2017,37(11):1222-1225.
[18]杨克红,刘浩,吴华璞,等.姜黄素预处理对大鼠脑缺血/再灌注损伤的保护作用[J].中国药理学通报,2013,29(10):1432-1436.
[19]徐丽丽,沈建军,胡智勇.姜黄素对脑缺血再灌注损伤的保护作用[J].中华医学杂志,2013,93(11):157-160.
[20] AGGARWAL B B, GUPTA S C, SUNG B. Curcumin:an orally bioavailable blocker of TNF and other pro-inflammatory biomarkers[J]. British Journal of Pharmacology, 2013, 169(8):1672-1692.
[2]张晶晶.利拉鲁肽对大鼠心肌再灌注损伤的作用及其机制[J].职业与健康,2016,32(1):45-47.
[3]王涵东,梁维邦.姜黄素的研究进展[J].江苏医药,2014,40(10):1193-1194.
[4]王彬辉,章文红,张晓芬,等.姜黄素的药理及剂型研究进展[J].中华中医药学刊,2013,31(5):1102-1105.
[5]张博,叶丽红.姜黄素抗癌机制研究进展[J].中医药学报,2013,41(1):121-122.
[6] TAKIKAWA M, SUMI Y, ISHIHARA M, et al. PRP&F/P MPs Improved Survival of Dorsal Paired Pedicle Skin Flaps in Rats[J]. Journal of Surgical Research, 2011, 170(1):189-196.
[7] YANG D, MORRIS S F. An extended dorsal island skin flap with multiple vascular territories in the rat:A new skin flap model[J]. The Journal of surgical research,1999,87(2):164-170.
[8]刘志辉,刘春丽,张伟.血管内皮细胞生长因子皮下注射对大鼠背部皮瓣成活的实验研究[J].口腔颌面外科杂志,2002,12(1):20-23.
[9]林泉.大鼠轴型皮瓣及其放射性损伤的低能量冲击波治疗的实验研究[D].长春:吉林大学,2010.
[10]赵珊,马迎春,刘亚坤,等.姜黄素通过抑制内质网应激和JNK通路过度活化减轻小鼠肺缺血再灌注损伤[J].中国病理生理杂志,2013,29(2):308-313.
[11]尤佳,戴东波,何雯洁,等.姜黄素长循环脂质体的制备及药动学研究[J].中国中药杂志,2014,39(7):1238-1242.
[12]何双艳,李珏宏,李昌平,等.姜黄素对DSS诱导的溃疡性结肠炎小鼠p38MAPK表达的影响[J].实用医学杂志,2014,30(4):539-541.
[13] GUPTA S C, KISMALI G, AGGARWAL B B. Curcumin, a component of turmeric:from farm to pharmacy[J]. Biofactors, 2013,39(1):2-13.
[14] WU J, LI Q, WANG X, et al. Neuroprotection by Curcumin in Ischemic Brain Injury Involves the Akt/Nrf2 Pathway[J]. Plos One, 2013, 8(3):e59843.
[15]王灵聪,张微,夏国莲,等.姜黄素对急性肺栓塞大鼠炎症因子的影响[J].中医杂志,2013,54(6):506-508.
[16]廖端芳,张彩平,顾洪丰,等.烟酸姜黄素脂通过调节细胞自噬和LDLR促进胆固醇逆向转运[C]//杭州:2016心血管药理专业委员会全国心脑血管药理学术会议,2016.
[17]孙四玉,杨冬梅,戴娜,等.乙酰水杨酸姜黄素酯对血管紧张素Ⅱ诱导的血管平滑肌细胞增殖的影响及相关机制[J].湖南中医药大学学报,2017,37(11):1222-1225.
[18]杨克红,刘浩,吴华璞,等.姜黄素预处理对大鼠脑缺血/再灌注损伤的保护作用[J].中国药理学通报,2013,29(10):1432-1436.
[19]徐丽丽,沈建军,胡智勇.姜黄素对脑缺血再灌注损伤的保护作用[J].中华医学杂志,2013,93(11):157-160.
[20] AGGARWAL B B, GUPTA S C, SUNG B. Curcumin:an orally bioavailable blocker of TNF and other pro-inflammatory biomarkers[J]. British Journal of Pharmacology, 2013, 169(8):1672-1692.