温盐交互胁迫下蒙古沙冬青生长及生理响应特征
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摘要
以蒙古沙冬青(Ammopiptanthus mongolicus)幼苗为材料,设定3个温度梯度(20、30、40℃)和6个Na Cl质量分数梯度(0、0.3%、0.6%、0.9%、1.2%、1.5%)处理,测定温盐交互胁迫30 d下蒙古沙冬青幼苗株高变化、比叶面积、光合速率、叶绿素质量分数、水势、叶片相对含水量、可溶性糖质量分数和脯氨酸质量分数等部分生长及生理指标。结果表明,温盐交互作用,尤其是高温和高盐胁迫同时存在时会更显著地对植株正常生长造成抑制。温盐交互胁迫对蒙古沙冬青幼苗株高变化、比叶面积、光合速率、叶片相对含水量、叶片水势和脯氨酸质量分数产生极显著抑制作用,对叶绿素质量分数有显著抑制作用,对可溶性糖质量分数没有显著影响,且高盐比高温胁迫对指标变化量的影响更大。
We studied the physiological and ecological indexes of Ammopiptanthus mongolicus seedlings under the interaction of temperature and NaCl stress. We set three temperature gradients( 20 ℃,30 ℃,and 40 ℃) and six Na Cl concentration gradients( 0,0.3%,0.6%,0.9%,1.2%,and 1.5%) to measure the changes of plant height,specific leaf area,photosynthetic rate,chlorophyll content,water potential,relative water content,soluble sugar content and proline content in seedlings under 30 d. The interaction of temperature and Na Cl,especially in the presence of high temperature and high NaCl stress,could significantly inhibit the normal growth of plants. Temperature and Na Cl stress in A. mongolicus seedlings change,specific leaf area,photosynthetic rate,leaf relative water content,leaf water potential and proline content had significant inhibitory effect,had obvious inhibitory effect on chlorophyll content,had no significant effect on soluble sugar content,and the effect of high salt stress on the change of index was greater than high temperature stress.
We studied the physiological and ecological indexes of Ammopiptanthus mongolicus seedlings under the interaction of temperature and NaCl stress. We set three temperature gradients( 20 ℃,30 ℃,and 40 ℃) and six Na Cl concentration gradients( 0,0.3%,0.6%,0.9%,1.2%,and 1.5%) to measure the changes of plant height,specific leaf area,photosynthetic rate,chlorophyll content,water potential,relative water content,soluble sugar content and proline content in seedlings under 30 d. The interaction of temperature and Na Cl,especially in the presence of high temperature and high NaCl stress,could significantly inhibit the normal growth of plants. Temperature and Na Cl stress in A. mongolicus seedlings change,specific leaf area,photosynthetic rate,leaf relative water content,leaf water potential and proline content had significant inhibitory effect,had obvious inhibitory effect on chlorophyll content,had no significant effect on soluble sugar content,and the effect of high salt stress on the change of index was greater than high temperature stress.
引文
[1]夏恩龙,施海,彭祚登.沙冬青抗逆性与培育技术研究进展[J].中国水土保持科学,2006,4(4):109-113.
[2]庞涛,郭丽丽,夏新莉,等.CBL1基因5'非翻译区内含子在旱生植物沙冬青中的作用[J].北京林业大学学报,2011,33(6):157-165.
[3]聂利珍,郭九峰,刘红葵,等.沙冬青脱水素基因转化紫花苜蓿的耐寒性研究[J].西北植物学报,2014,34(9):1727-1734.
[4]李章磊,高飞,曹玉震,等.蒙古沙冬青Am DREB2.1基因的克隆及表达分析[J].生物技术通报,2015,31(3):108-114.
[5]郭婷,王茅雁,董博,等.蒙古沙冬青Am DREB2C基因的克隆及表达分析[J].植物遗传资源学报,2015,16(2):344-348.
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[9]丁志强,尚桂军,李娜,等.沙冬青CBL1蛋白纯化及性质研究[J].中国生物工程杂志,2011,31(2):23-29.
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[11]YUAN W J,YU Y,YUE Y D,et al.Use of infrared thermal imaging to diagnose health of Ammopiptanthus mongolicus in northwestern China[J].For.Res,2015,26(3):605-612.
[12]GUO H M,LI Z C,ZHOU M L,et al.c DNA-AFLP analysis reveals heat shock proteins play important roles in mediating cold,heat,and drought tolerance in Ammopiptanthus mongolicus[J].Funct Integr Genomics,2014,14(1):127-133.
[13]于军,焦培培.聚乙二醇(PEG6000)模拟干旱胁迫抑制矮沙冬青种子的萌发[J].基因组学与应用生物学,2010,29(2):355-360.
[14]段慧荣,李毅,马彦军.PEG胁迫对沙冬青种子萌发过程的影响[J].水土保持研究,2011,18(3):221-225.
[15]张娇,张大治,马艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014(14):65-72.
[16]ZHOU Y J,GAO F,LIU R,et al.De novo sequencing and analysis of root transcriptome using 454 pyrosequencing to discover putative genes associated with drought tolerance in Ammopiptanthus mongolicus[J].BMC Genomics,2012,13(1):266.
[17]张谧,王慧娟,于长青.超旱生植物沙冬青高温胁迫下的快速叶绿素荧光动力学特征[J].生态环境学报,2009,18(6):2272-2277.
[18]WEI Q,HU P,KUAI B K.Ectopic expression of an Ammopiptanthus mongolicus H+-pyrophosphatase gene enhances drought and salt tolerance in Arabidopsis[J].Plant Cell Tiss Organ Cult,2012,110(1):359-369.
[19]曹小曙,李平,颜廷真,等.近百年来西辽河流域土地开垦及其对环境的影响[J].地理研究,2005,24(6):889-898.
[20]张莹,何炎红,田有亮.强光和水分胁迫对沙冬青光呼吸的影响[J].安徽农业科学,2013,41(24):10027-10030.
[21]蒋志荣.沙冬青抗旱机理的探讨[J].中国沙漠,2000,20(1):71-74.
[22]张党权,宋志丹,田晔林.抗逆模式灌木沙冬青的研究进展[J].中南林业科技大学学报.2012,32(2):16-20.
[23]李婧男,刘强,李升.生长素和氯化钙对盐胁迫下沙冬青幼苗的缓解作用[J].植物研究,2010,30(1):27-31.
[24]刘敏,厉悦,毛子军,等.盐分和温度对万寿菊种子发芽的影响[J].草业科学,2014,31(6):1104-1109.
[25]胡月楠,贺康宁,王占林,等.盐胁迫对霸王水势的影响[J].中国沙漠,2009,29(5):905-910.
[26]蔺吉祥,李晓宇,张兆军,等.温度与盐碱胁迫交互作用对羊草种子萌发与幼苗生长的影响[J].草地学报,2011,19(6):1005-1009.
[27]刘生龙.在干旱沙区适宜引种的野生花卉[J].植物杂志,1992,2(2):17.
[28]麦尔哈巴·阿布拉.沙冬青植被特征与干旱环境的关系[D].北京:中央民族大学,2015.
[29]夏恩龙.沙冬青地理分布与在北京地区引种栽培技术的研究[D].北京:北京林业大学,2006.
[30]宋祥春,赵惠新,苗玉,等.低温对两种沙冬青幼苗光合生理指标的影响[J].新疆大学学报(自然科学版),2009,26(3):342-346.
[31]李婧男,刘强,贾志宽,等.盐胁迫对沙冬青幼苗生长与生理特性的影响[J].植物研究,2009,29(5):553-558.
[32]赵峰侠,尹林克,牟书勇.新疆沙冬青(Ammopiptanthus nanus)渗透调节物质的季节变化与环境因子的关系[J].干旱区地理,2008,121(5):665-672.
[33]王华,贾桂霞,丁琼.沙冬青抗逆性研究进展与应用前景[J].中国农学通报,2005,21(12):121-125.
[34]郭华军.水分胁迫过程中的渗透调节物质及其研究进展[J].安徽农业科学,2010,38(15):7750-7753,7760.
[2]庞涛,郭丽丽,夏新莉,等.CBL1基因5'非翻译区内含子在旱生植物沙冬青中的作用[J].北京林业大学学报,2011,33(6):157-165.
[3]聂利珍,郭九峰,刘红葵,等.沙冬青脱水素基因转化紫花苜蓿的耐寒性研究[J].西北植物学报,2014,34(9):1727-1734.
[4]李章磊,高飞,曹玉震,等.蒙古沙冬青Am DREB2.1基因的克隆及表达分析[J].生物技术通报,2015,31(3):108-114.
[5]郭婷,王茅雁,董博,等.蒙古沙冬青Am DREB2C基因的克隆及表达分析[J].植物遗传资源学报,2015,16(2):344-348.
[6]SUN J,NIE L Z,SUNG Q,et al.Cloning and characterization of dehydrin gene from Ammopiptanthus mongolicus[J].Mol Biol Rep,2013,10(40):2281-2291.
[7]GULJ,ZHANG H,GUO H M,et al.Cloning of Ammopiptanthus mongolicus C-repeat-binding factor gene and Its cold-induced tolerance in transgenic tobacco[J].Brazilian Archives of Biology and Technology,2013,56(3):1516-8913.
[8]SHI J,LIU M Q,SHI J N,et al.Reference gene selection for q PCR in Ammopiptanthus mongolicus under abiotic stresses and expression analysis of seven ROS-scavenging enzyme genes[J].Plant Cell Rep,2012,31(7):1245-1254.
[9]丁志强,尚桂军,李娜,等.沙冬青CBL1蛋白纯化及性质研究[J].中国生物工程杂志,2011,31(2):23-29.
[10]GUL J,CHENG H M.Isolation,molecular cloning and characterization of a cold-responsive gene,Am DUF1517,from Ammopiptanthus mongolicus[J].Plant Cell Tiss Organ Cult,2014,117(11):201-211.
[11]YUAN W J,YU Y,YUE Y D,et al.Use of infrared thermal imaging to diagnose health of Ammopiptanthus mongolicus in northwestern China[J].For.Res,2015,26(3):605-612.
[12]GUO H M,LI Z C,ZHOU M L,et al.c DNA-AFLP analysis reveals heat shock proteins play important roles in mediating cold,heat,and drought tolerance in Ammopiptanthus mongolicus[J].Funct Integr Genomics,2014,14(1):127-133.
[13]于军,焦培培.聚乙二醇(PEG6000)模拟干旱胁迫抑制矮沙冬青种子的萌发[J].基因组学与应用生物学,2010,29(2):355-360.
[14]段慧荣,李毅,马彦军.PEG胁迫对沙冬青种子萌发过程的影响[J].水土保持研究,2011,18(3):221-225.
[15]张娇,张大治,马艳,等.氯化钠模拟盐胁迫对沙冬青种子萌发和幼苗生长的影响[J].北方园艺,2014(14):65-72.
[16]ZHOU Y J,GAO F,LIU R,et al.De novo sequencing and analysis of root transcriptome using 454 pyrosequencing to discover putative genes associated with drought tolerance in Ammopiptanthus mongolicus[J].BMC Genomics,2012,13(1):266.
[17]张谧,王慧娟,于长青.超旱生植物沙冬青高温胁迫下的快速叶绿素荧光动力学特征[J].生态环境学报,2009,18(6):2272-2277.
[18]WEI Q,HU P,KUAI B K.Ectopic expression of an Ammopiptanthus mongolicus H+-pyrophosphatase gene enhances drought and salt tolerance in Arabidopsis[J].Plant Cell Tiss Organ Cult,2012,110(1):359-369.
[19]曹小曙,李平,颜廷真,等.近百年来西辽河流域土地开垦及其对环境的影响[J].地理研究,2005,24(6):889-898.
[20]张莹,何炎红,田有亮.强光和水分胁迫对沙冬青光呼吸的影响[J].安徽农业科学,2013,41(24):10027-10030.
[21]蒋志荣.沙冬青抗旱机理的探讨[J].中国沙漠,2000,20(1):71-74.
[22]张党权,宋志丹,田晔林.抗逆模式灌木沙冬青的研究进展[J].中南林业科技大学学报.2012,32(2):16-20.
[23]李婧男,刘强,李升.生长素和氯化钙对盐胁迫下沙冬青幼苗的缓解作用[J].植物研究,2010,30(1):27-31.
[24]刘敏,厉悦,毛子军,等.盐分和温度对万寿菊种子发芽的影响[J].草业科学,2014,31(6):1104-1109.
[25]胡月楠,贺康宁,王占林,等.盐胁迫对霸王水势的影响[J].中国沙漠,2009,29(5):905-910.
[26]蔺吉祥,李晓宇,张兆军,等.温度与盐碱胁迫交互作用对羊草种子萌发与幼苗生长的影响[J].草地学报,2011,19(6):1005-1009.
[27]刘生龙.在干旱沙区适宜引种的野生花卉[J].植物杂志,1992,2(2):17.
[28]麦尔哈巴·阿布拉.沙冬青植被特征与干旱环境的关系[D].北京:中央民族大学,2015.
[29]夏恩龙.沙冬青地理分布与在北京地区引种栽培技术的研究[D].北京:北京林业大学,2006.
[30]宋祥春,赵惠新,苗玉,等.低温对两种沙冬青幼苗光合生理指标的影响[J].新疆大学学报(自然科学版),2009,26(3):342-346.
[31]李婧男,刘强,贾志宽,等.盐胁迫对沙冬青幼苗生长与生理特性的影响[J].植物研究,2009,29(5):553-558.
[32]赵峰侠,尹林克,牟书勇.新疆沙冬青(Ammopiptanthus nanus)渗透调节物质的季节变化与环境因子的关系[J].干旱区地理,2008,121(5):665-672.
[33]王华,贾桂霞,丁琼.沙冬青抗逆性研究进展与应用前景[J].中国农学通报,2005,21(12):121-125.
[34]郭华军.水分胁迫过程中的渗透调节物质及其研究进展[J].安徽农业科学,2010,38(15):7750-7753,7760.