藏药榜嘎中黄酮类化学成分的研究
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摘要
目的:研究毛茛科乌头属植物榜嘎地上部分的黄酮类成分。方法:采用大孔吸附树脂富集,正相硅胶柱层析色谱法初分,通过葡聚糖凝胶Sephadex LH-20和半制备高压液相色谱法对各特征峰进行精制分离,根据其理化性质和NMR、MS等波谱数据鉴定化合物结构。结果:从榜嘎70%甲醇提取物中分离得到6个黄酮苷类化合物,分别鉴定为Clovin(1)、刺槐苷(2)、榜嘎苷A(3)、榜嘎苷B(4)、榜嘎苷C(5)、榜嘎苷D(6)。结论:化合物1、2为首次从榜嘎中分离得到,化合物3~6为新黄酮类化合物。
Objective:To investigate the flavonoids in bangga,aerial part of Aconitum naviculare(Bruhl.)Stapf.Methods:The compounds were separated and purified by silica gel and Sephadex LH-20 gel column chromatography as well as pre-HPLC.Their structures were identified by spectral analysis and chemical methods.Results:Six compounds were isolated from the 70% methanol extract of bangga.On the basis of the spectral data,their structures were identified as Clovin(1),robinin(2),bangga flavonoid glycoside A(3),bangga flavonoid glycoside B(4),bangga flavonoid glycoside C(5)and bangga flavonoid glycoside D(6).Conclusion:Compound 1 and compound 2 were isolated from bangga for the first time,and compound 3,4,5 and 6 were new flavonoids and named as bangga flavonoid glycoside A,bangga flavonoid glycoside B,bangga flavonoid glycoside C and bangga flavonoid glycoside D,respectively.
Objective:To investigate the flavonoids in bangga,aerial part of Aconitum naviculare(Bruhl.)Stapf.Methods:The compounds were separated and purified by silica gel and Sephadex LH-20 gel column chromatography as well as pre-HPLC.Their structures were identified by spectral analysis and chemical methods.Results:Six compounds were isolated from the 70% methanol extract of bangga.On the basis of the spectral data,their structures were identified as Clovin(1),robinin(2),bangga flavonoid glycoside A(3),bangga flavonoid glycoside B(4),bangga flavonoid glycoside C(5)and bangga flavonoid glycoside D(6).Conclusion:Compound 1 and compound 2 were isolated from bangga for the first time,and compound 3,4,5 and 6 were new flavonoids and named as bangga flavonoid glycoside A,bangga flavonoid glycoside B,bangga flavonoid glycoside C and bangga flavonoid glycoside D,respectively.
引文
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[16]MARIANI C,BRACA A,VITALINI S,et al.Flavonoid characterization and in vitro antioxidant activity of Aconitum anthora L.(Ranunculaceae)[J].Phytochemistry,2008,69(5):1220
[17]TANAKA T,NAKASHIMA T,UEDA T,et al.Facile discrimination of aldose enantiomers by reversed-phase HPLC[J].Chem Pharm Bull,2007,55:899
[18]肖崇厚.中药化学[M].上海:上海科学技术出版社,1997:309XIAO CH.Chemistry of Traditional Chinese Medicine[M].Shanghai:Shanghai Science and Technology Publishers,1997:309
[19]WANG SS,ZHANG XJ,QUE S,et al.3-hydroxy-3-methylglutaryl flavonol glycosides from Oxytropis falcate[J].J Nat Prod,2012,75:1359
[20]MARIANI C,BRACA A,VITALINI S,et al.Flavonoid characterization and in vitro antioxidant activity of Aconitum anthora L.(Ranunculaceae)[J].Phytochemistry,2008,69(5):1220
[21]BLUNDER M,ORTHABER A,BAUER R,et al.Efficient identification of flavones,flavanones and their glycosides in routine analysis via off-line combination of sensitive NMR and HPLC experiments[J].Food Chem,2017,218:600
[2]罗达尚.新修晶珠本草[M].四川:四川科学科技出版社,2003LUO DS.Xin Xiu Jing Zhu Ben Cao[M].Sichuan:Sichuan Science and Technology Publishing House,2004:245
[3]刘治民.藏药榜嘎、榜那的资源调查和药用合理性评价[D].北京:北京中医药大学,2014:245LIU ZM.Resource Investigation and Medicinal Rationality of Tibetan Medicine Bangga and Baligna[D].Beijing:Beijing Univ ersity Chinese Medicine,2013
[4]卫生部药品标准·藏药.第一册[S].1995:85Drug Specification Promulgated by the Ministry of Public Healt.P R China.Tibetan Medicine.VolⅠ[S].1995:85
[5]罗明,李春,林丽美,等.藏药榜嘎化学成分和药理作用的研究进展[J].中国实验方剂学,2012,18(12):299.LUO M,LI C,LIN LM,et al.Research progress on Tibetan medicinal herb ponkar[J].Chin J Exp Tradit Med Form,2012,18(12):299
[6]赵翡翠,李杰.我国乌头属药用植物中生物碱分析方法的研究进展[J].中药材,2010,33(8):1351ZHAO FC,LI J.Recent progress in research analysis of alkaloid in aconitum plants[J].J Chin Med Mater,2010,33(8):1351
[7]贾庆文,闫滨,王璐.乌头类药物的毒性研究及唐古特乌头的研究概述[J].食品与药品,2012,14(7):300JIA QW,YAN B,WANG L.Toxicity study on aconitum drug and overview on Aconitum tanguticum(Maxim.)Stapf[J].Food Drug,2012,14(7):300
[8]康慧,骆桂法,杨凤梅,等.藏药榜嘎中的双酯型和内酯型生物碱分析方法研究[J].西北药学杂志,2014,29(5):443KANG H,LUO GF,YANG FM,et al.Study on the analytical method of diester-type alkaloids in Tibetan medicine Bangga[J].J Northwest Pharm,2014,29(5):443
[9]李艳茸,李春,王智明,等.藏药甘青乌头化学成分研究(Ⅲ)[J].中国中药杂志,2014,39(7):1163LI YR,LI C,WANG ZM,et al.Chemical constituents from whole plants of Aconitum tanguticum(Ⅲ)[J].China J Chin Mater Med,2014,39(7):1163
[10]徐璐,李艳茸,李春,等.藏药甘青乌头化学成分研究[J].中国中药杂志,2013,38(17):2818XU L,LI YR,LI C,et al.Chemical constituents from whole plants of Aconitum tanguticum[J].China J Chin Mater Med,2013,38(17):2818
[11]罗明,林丽美,李春,等.藏药甘青乌头化学成分研究[J].中国中药杂志,2012,37(9):1245LUO M,LIN LM,LI C,et al.Chemical constituents of Aconitum tanguticum[J].China J Chin Mater Med,2012,37(9):1245
[12]SCHAUFELBERGER D,GUPTA MP,HOSTETTMANN K.Flavonol and secoiridoid glycosides from coutoubea spicata[J].Phytochemistry,1987,26(8):2377
[13]LIU Q,LIU M,MABRY TJ,et al.Flavonol glycosides from cephalocereus senilis[J].Phytochemistry,1994,36(1):229
[14]叶敏,阎玉凝,乔梁,等.中药菟丝子化学成分研究[J].中国中药杂志,2002,27(2):115YE M,YAN YN,QIAO L,et al.Studies on chemical constituents of cuscuta chinensis[J].China J Chin Mater Med,2002,27(2):115
[15]SHRESTHA BB,DALL’ACQUA S,GEWALI MB,et al.New flavonoid glycosides from Aconitum naviculare(Brühl)Stapf,a medicinal herb from the trans-himalayan region of nepal[J].Carbohydr Res,2006,341(12):2161
[16]MARIANI C,BRACA A,VITALINI S,et al.Flavonoid characterization and in vitro antioxidant activity of Aconitum anthora L.(Ranunculaceae)[J].Phytochemistry,2008,69(5):1220
[17]TANAKA T,NAKASHIMA T,UEDA T,et al.Facile discrimination of aldose enantiomers by reversed-phase HPLC[J].Chem Pharm Bull,2007,55:899
[18]肖崇厚.中药化学[M].上海:上海科学技术出版社,1997:309XIAO CH.Chemistry of Traditional Chinese Medicine[M].Shanghai:Shanghai Science and Technology Publishers,1997:309
[19]WANG SS,ZHANG XJ,QUE S,et al.3-hydroxy-3-methylglutaryl flavonol glycosides from Oxytropis falcate[J].J Nat Prod,2012,75:1359
[20]MARIANI C,BRACA A,VITALINI S,et al.Flavonoid characterization and in vitro antioxidant activity of Aconitum anthora L.(Ranunculaceae)[J].Phytochemistry,2008,69(5):1220
[21]BLUNDER M,ORTHABER A,BAUER R,et al.Efficient identification of flavones,flavanones and their glycosides in routine analysis via off-line combination of sensitive NMR and HPLC experiments[J].Food Chem,2017,218:600