6种含氮化合物对Na_2CO_3胁迫下紫花苜蓿种子萌发特性的影响
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摘要
研究不同类型含氮化合物对碱性盐Na2CO3胁迫下紫花苜蓿种子萌发特性的影响。试验设6种含氮化合物,各8种浓度,计算发芽率指标。结果显示:各含氮化合物处理均未能显著缓解25 mmol/L Na2CO3的萌发抑制效应,部分处理接近或超出对照水平。除NH4H2PO4外,其他化合物高浓度处理显著抑制萌发。80 mmol/L NH4H2PO4(pH 6.63)处理下,前期发芽、恢复发芽及总发芽均高于对照,尽管统计意义上不显著。结果进一步暗示萌发阶段盐胁迫效应与pH关系密切。需要更广泛的试验来确定盐碱地改良作物生产时"治盐先治碱"或"治盐须治碱"的可行性。
The research aimed to investigate the effects of various nitrogenous compounds on seed germinationof Medicago sativa L. under basic Na2CO3 stress. Six nitrogenous compounds were used in the experiment, eachat eight concentrations. Seed germination percentage was calculated. The result showed that none of thenitrogenous compounds could significantly alleviate the inhibitory effect of 25 mmol/L Na2CO3 on seedgermination of M. sativa. Several treatments approached or exceeded the control. Treatments with all testedcompounds except NH4H2PO4 at higher concentrations decreased the germination significantly. Earlygermination, recovery germination and total germination under treatment with 80 mmol/L NH4H2PO4(pH 6.63)were higher than those under the control, not significant statistically. These results further indicated that stresseffect of salt was closely related with pH. More extensive trials were needed to explore the possibility ofameliorating soil salinity based on alkaline decrease in improving crop production in saline-alkali soil.
The research aimed to investigate the effects of various nitrogenous compounds on seed germinationof Medicago sativa L. under basic Na2CO3 stress. Six nitrogenous compounds were used in the experiment, eachat eight concentrations. Seed germination percentage was calculated. The result showed that none of thenitrogenous compounds could significantly alleviate the inhibitory effect of 25 mmol/L Na2CO3 on seedgermination of M. sativa. Several treatments approached or exceeded the control. Treatments with all testedcompounds except NH4H2PO4 at higher concentrations decreased the germination significantly. Earlygermination, recovery germination and total germination under treatment with 80 mmol/L NH4H2PO4(pH 6.63)were higher than those under the control, not significant statistically. These results further indicated that stresseffect of salt was closely related with pH. More extensive trials were needed to explore the possibility ofameliorating soil salinity based on alkaline decrease in improving crop production in saline-alkali soil.
引文
[1]刘寅哲,张义.不同温度下复合盐胁迫及恢复对紫花苜蓿种子萌发特性的影响[J].种子,2009,28(11):22-24.
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[3]贾广云,张博,王玉祥,等.NaCl胁迫对苦马豆种子萌发的影响及生理效应[J].中国农学通报,2009,25(8):181-184.
[4]Khadri M,Tejera N A,Lluch C.Sodium chloride-ABA interaction in two common bean(Phaseolus vulgaris)cultivars differing in salinity tolerance[J].Environmental and Experimental Botany,2007,60:211-218.
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[8]Cao J,Li X T,Kong X L,et al.Using alfalfa(Medicago sativa)to ameliorate salt-affected soils in Yingda irrigation district inNorthwest China[J].Acta Ecologica Sinica.2012,32:68-73.
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[11]韩清芳,李崇巍,贾志宽.不同苜蓿品种种子萌发期耐盐性的研究[J].西北植物学报,2003,23(4):597-602.
[12]沈振荣,杨万仁,徐秀梅.不同盐分胁迫对苜蓿种子萌发的影响[J].种子,2006,25(4):34-37.
[13]Basalah M O,Mohammad S.Effect of salinity and plant growth regulators on seed germination of Medicago sativa L.[J].Pakistan Journal of Biological Sciences,1999,2(3):651-653.
[14]Li R L,Shi F C,Fukuda K,et al.Effects of salt and alkali stresses on germination,growth,photosynthesis and ion accumulation in alfalfa(Medicago sativa L.)[J].Soil Science and Plant Nutrition,2010,56:725-733.
[15]许国经,汪景波,张义.短期NaCl与Na2CO3胁迫对紫花苜蓿种子萌发可恢复性的影响[J].中国农学通报,2011,27(27):109-112.
[16]Gul B,Khan M A.Effect of compatible osmotica and plant growth regulators in alleviating salinity stress on the seed germination of Allenrolfea occidentalis[J].Pakistan Journal of Botany,2008,40(5):1957-1964.
[17]Shahba M A,Qian Y L,Lair K D.Improving seed germination of saltgrass under saline conditions[J].Crop Science,2008,48:756-762.
[18]Atia A,Debez A,Barhoumi Z,et al.ABA,GA3,and nitrate may control seed germination of Crithmum maritimum(Apiaceae)under saline conditions[J].C.R.Biologies,2009,332:704-710.
[19]王大昊,王旭,唐娇,等.不同含氮化合物对NaCl胁迫下紫花苜蓿种子萌发特性的影响[J].中国农学通报,2014,30(5):19-22.
[20]Neid S L,Biesboer D D.Alleviation of salt-induced stress on seed emergence using soil additives in a greenhouse[J].Plant and Soil,2005,268:303-307.
[21]Khan M A,Gul B,Weber D J.Effect of germination regulating chemicals on seed germination of Halogeton glomeratus for alleviation of salinity stress[J].Pakistan Journal of Botany,2009,41(3):1205-1212.
[22]崔玮,张芬琴,李玉兰,等.中性盐和碱性盐胁迫对黄瓜种子萌发的影响[J].种子,2006,25(4):66-69.
[23]魏晓斌,徐安凯,王志锋,等.不同pH值土壤浸提液对苜蓿种子发芽和幼苗生长的影响[J].中国农学通报,2013,29(15):60-67.
[2]Qu X X,Baskin J M,Wang L,et al.Effects of cold stratification,temperature,light and salinity on seed germination and radicle growth of the desert halophyte shrub,Kalidium capsicum(Chenopodiaceae)[J].Plant Growth Regulation,2008(54):241-248.
[3]贾广云,张博,王玉祥,等.NaCl胁迫对苦马豆种子萌发的影响及生理效应[J].中国农学通报,2009,25(8):181-184.
[4]Khadri M,Tejera N A,Lluch C.Sodium chloride-ABA interaction in two common bean(Phaseolus vulgaris)cultivars differing in salinity tolerance[J].Environmental and Experimental Botany,2007,60:211-218.
[5]赵可夫,范海.盐生植物及其对盐渍生境的适应生理[M].北京:科学出版社,2005.
[6]郗金标,张福锁,田长彦.新疆盐生植物[M].北京:科学出版社,2006.
[7]颜宏,矫爽,赵伟,等.不同大小碱地肤种子的萌发耐盐性比较[J].草业学报,2008,17(2):26-32.
[8]Cao J,Li X T,Kong X L,et al.Using alfalfa(Medicago sativa)to ameliorate salt-affected soils in Yingda irrigation district inNorthwest China[J].Acta Ecologica Sinica.2012,32:68-73.
[9]李源,刘贵波,高洪文,等.紫花苜蓿种质耐盐性综合评价及盐胁迫下的生理反应[J].草业学报,2010,19(4):79-86.
[10]高战武,王德利.紫花苜蓿对复合盐碱胁迫的适应性响应[D].长春:东北师范大学,2006.
[11]韩清芳,李崇巍,贾志宽.不同苜蓿品种种子萌发期耐盐性的研究[J].西北植物学报,2003,23(4):597-602.
[12]沈振荣,杨万仁,徐秀梅.不同盐分胁迫对苜蓿种子萌发的影响[J].种子,2006,25(4):34-37.
[13]Basalah M O,Mohammad S.Effect of salinity and plant growth regulators on seed germination of Medicago sativa L.[J].Pakistan Journal of Biological Sciences,1999,2(3):651-653.
[14]Li R L,Shi F C,Fukuda K,et al.Effects of salt and alkali stresses on germination,growth,photosynthesis and ion accumulation in alfalfa(Medicago sativa L.)[J].Soil Science and Plant Nutrition,2010,56:725-733.
[15]许国经,汪景波,张义.短期NaCl与Na2CO3胁迫对紫花苜蓿种子萌发可恢复性的影响[J].中国农学通报,2011,27(27):109-112.
[16]Gul B,Khan M A.Effect of compatible osmotica and plant growth regulators in alleviating salinity stress on the seed germination of Allenrolfea occidentalis[J].Pakistan Journal of Botany,2008,40(5):1957-1964.
[17]Shahba M A,Qian Y L,Lair K D.Improving seed germination of saltgrass under saline conditions[J].Crop Science,2008,48:756-762.
[18]Atia A,Debez A,Barhoumi Z,et al.ABA,GA3,and nitrate may control seed germination of Crithmum maritimum(Apiaceae)under saline conditions[J].C.R.Biologies,2009,332:704-710.
[19]王大昊,王旭,唐娇,等.不同含氮化合物对NaCl胁迫下紫花苜蓿种子萌发特性的影响[J].中国农学通报,2014,30(5):19-22.
[20]Neid S L,Biesboer D D.Alleviation of salt-induced stress on seed emergence using soil additives in a greenhouse[J].Plant and Soil,2005,268:303-307.
[21]Khan M A,Gul B,Weber D J.Effect of germination regulating chemicals on seed germination of Halogeton glomeratus for alleviation of salinity stress[J].Pakistan Journal of Botany,2009,41(3):1205-1212.
[22]崔玮,张芬琴,李玉兰,等.中性盐和碱性盐胁迫对黄瓜种子萌发的影响[J].种子,2006,25(4):66-69.
[23]魏晓斌,徐安凯,王志锋,等.不同pH值土壤浸提液对苜蓿种子发芽和幼苗生长的影响[J].中国农学通报,2013,29(15):60-67.