荆芥对4种葡萄病原真菌的抑制作用
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摘要
为了有效利用荆芥资源,采用菌丝生长速率法研究了荆芥醇提物对4种葡萄果实病原真菌的抑制作用.结果表明,当醇提物质量浓度为5 mg/mL时,对白腐病菌(Coniothyrium diplodiella)、炭疽病菌(Glomerella cingulate)、灰霉病菌(Botrytis cinerea)和炭黑曲霉病菌(Aspergillus carbonarius)的抑制率分别为94.57%、70.23%、71.15%和26.06%,对多菌灵高抗炭疽病菌菌株FJND-9、FJND-40和GZSD-89的抑制率分别为64.10%、68.73%和72.94%,对多菌灵高抗灰霉病菌菌株HBLF-10、GXZY-4和GXZY-7的抑制率分别为57.47%、66.83%和64.90%.本研究为葡萄病害非化学农药防控提供了依据.
For utilization of the resources of Nepeta cataria, the bioassay of ethanol extract against 4 pathogenic fungi isolated from grapes were carried out by mycelial growth rate method. The results showed that when the concentration of extract was 5.0 mg/mL, the inhibition rate against Coniothyrium diplodiella, Glomerella cingulate, Botrytis cinerea and Aspergillus carbonarius was 94.57%, 70.23%, 71.15% and 26.06%, respectively. The inhibition rate against carbendazim high resistant strains FJND-9, FJND-40 and GZSD-89 of G. cingulate was 64.10%, 68.73% and 72.94%,and the inhibition rate against carbendazim high resistant strains HBLF-10, GXZY-4 and GXZY-7 of B. cinerea was 57.47%, 66.83% and 64.90%, respectively, when ethanol extract was at same concentration. This study provides a basis for the prevention and control of grape diseases using non chemical pesticides.
For utilization of the resources of Nepeta cataria, the bioassay of ethanol extract against 4 pathogenic fungi isolated from grapes were carried out by mycelial growth rate method. The results showed that when the concentration of extract was 5.0 mg/mL, the inhibition rate against Coniothyrium diplodiella, Glomerella cingulate, Botrytis cinerea and Aspergillus carbonarius was 94.57%, 70.23%, 71.15% and 26.06%, respectively. The inhibition rate against carbendazim high resistant strains FJND-9, FJND-40 and GZSD-89 of G. cingulate was 64.10%, 68.73% and 72.94%,and the inhibition rate against carbendazim high resistant strains HBLF-10, GXZY-4 and GXZY-7 of B. cinerea was 57.47%, 66.83% and 64.90%, respectively, when ethanol extract was at same concentration. This study provides a basis for the prevention and control of grape diseases using non chemical pesticides.
引文
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[10]李晶,李娜,丁品,等.黄帚橐吾提取物对保护地辣椒4种病原真菌的抑制活性及其病害防效[J].草业学报,2018,27(4):56-68.DOI:10.11686/cyxb2017204.
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[13]朱梅芳,唐宇,郑琴,等.不同提取方式对连翘、荆芥、薄荷挥发油成分及抗菌活性的影响[J].中草药,2018,49(12):2845-2854.DOI:10.7501/j.issn.0253-2670.2018.12.017.
[14]任云,李明清,郭庆梅,等.冀南荆芥挥发油提取及其抑菌性能研究[J].食品科技,2017,42(2):232-235.DOI:10.13684/j.cnki.spkj.2017.02.045.
[15]WILSON C L,SOLAR J M,El GHAOUTH A,et al.Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea[J].Plant Disease,1997,81(2):204-210.
[16]SINDHU S,CHEMPAKAM B,LEELA N K,et al.Chemoprevention by essential oil of turmeric leaves(Curcuma longaL.)on the growth of Aspergillus flavus and aflatoxin production[J].Food and Chemical Toxicology,2011,49(5):1188-1192.DOI:org/10.1016/j.fct.2011.02.014.
[17]PINTO J M A,SOUZA E A,OLIVEIRA D F.Use of plant extracts in the control of common bean anthracnose[J].Crop Protection,2010,29(8):838-842.DOI:org/10.1016/j.cropro.2010.03.006.
[18]TALIBI I,ASKARNE L,BOUBAKER H,et al.Antifungal activity of some Moroccan plants against Geotrichum candidum,the causal agent of postharvest citrus sour rot[J].Crop Protection,2012,35:41-46.DOI:org/10.1016/j.cropro.2011.12.016.
[2]钱恒伟,赵宇,黄金光.青岛地区葡萄灰霉病病原鉴定及病害分析[J].山东农业科学,2015,47(7):87-90.DOI:10.14083/j.issn.1001-4942.2015.07.019.
[3]宋雅琴,娄兵海,陈爱军,等.5种杀菌剂对广西葡萄灰霉病病菌的毒力评价[J].中国南方果树,2017,46(3):132-134.DOI:10.13938/j.issn.1007-1431.20160624.
[4]粟国英,王长荣.7种杀菌剂对葡萄灰霉病的防治效果探究[J].农业技术与装备,2017,327(3):61-62.DOI:10.3969/j.issn.1673-887X.2017.03.028.
[5]孙行杰,刘保友,王英姿.6种杀菌剂对葡萄炭疽病菌的毒力测定[J].北方果树,2016(1):5-6.DOI:10.16376/j.cnki.bfgs.2016.01.002.
[6]翟浩,王涛,李晓军,等.不同杀菌剂对葡萄炭疽病的防治效果[J].安徽农业科学,2015,43(35):190.DOI:10.13989/j.cnki.0517-6611.2015.35.069.
[7]宗宇,吴志娟,朱友银,等.葡萄炭疽病病原菌分离鉴定及其效用杀菌剂筛选[J].浙江师范大学学报(自然科学版),2017,40(4):435-440.DOI:10.16218/j.issn.1001-5051.2017.04.012.
[8]申芬,蒋继志,侯宁,等.5种拮抗菌复合发酵对致病疫霉的抑制及离体防病作用研究[J].河北农业大学学报,2018,41(1):11-16.DOI:10.13320/j.cnki.jauh.2018.0003.
[9]王游游,蒋继志,李颖,等.6株拮抗细菌对致病疫霉抑制作用的比较[J].河北大学学报(自然科学版),2017,37(2):169-175.DOI:10.3969/j.issn.1000-1565.2017.02.010.
[10]李晶,李娜,丁品,等.黄帚橐吾提取物对保护地辣椒4种病原真菌的抑制活性及其病害防效[J].草业学报,2018,27(4):56-68.DOI:10.11686/cyxb2017204.
[11]方丽平,李进步,薛建平,等.六十种药用植物提取物对葡萄炭疽病菌抑菌活性的室内筛选[J].北方园艺,2014(2):119-123.
[12]中科院中国植物志编委会.中国植物志:第65(2)卷[M].北京:科学出版社,1977:298.
[13]朱梅芳,唐宇,郑琴,等.不同提取方式对连翘、荆芥、薄荷挥发油成分及抗菌活性的影响[J].中草药,2018,49(12):2845-2854.DOI:10.7501/j.issn.0253-2670.2018.12.017.
[14]任云,李明清,郭庆梅,等.冀南荆芥挥发油提取及其抑菌性能研究[J].食品科技,2017,42(2):232-235.DOI:10.13684/j.cnki.spkj.2017.02.045.
[15]WILSON C L,SOLAR J M,El GHAOUTH A,et al.Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea[J].Plant Disease,1997,81(2):204-210.
[16]SINDHU S,CHEMPAKAM B,LEELA N K,et al.Chemoprevention by essential oil of turmeric leaves(Curcuma longaL.)on the growth of Aspergillus flavus and aflatoxin production[J].Food and Chemical Toxicology,2011,49(5):1188-1192.DOI:org/10.1016/j.fct.2011.02.014.
[17]PINTO J M A,SOUZA E A,OLIVEIRA D F.Use of plant extracts in the control of common bean anthracnose[J].Crop Protection,2010,29(8):838-842.DOI:org/10.1016/j.cropro.2010.03.006.
[18]TALIBI I,ASKARNE L,BOUBAKER H,et al.Antifungal activity of some Moroccan plants against Geotrichum candidum,the causal agent of postharvest citrus sour rot[J].Crop Protection,2012,35:41-46.DOI:org/10.1016/j.cropro.2011.12.016.