镉胁迫对甘蔗抗氧化酶系统及非蛋白巯基物质的影响
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摘要
为了解镉胁迫下甘蔗的生理生化响应,通过桶栽试验模拟甘蔗在镉胁迫下生长,探讨镉胁迫的不同浓度和不同时期对甘蔗生理生化的影响。结果表明:镉处理对甘蔗叶绿素均有不同程度的影响,随着时间的推移叶绿素变化得更为明显;对于清除活性氧系统酶类,包括超氧化物歧化酶(SOD)和过氧化物酶(POD),相比于对照则出现了较大的波动;脯氨酸(PRO)和丙二醛(MDA)含量受镉浓度影响较小,在镉处理前期并未有明显的变化,但在处理中后期出现了明显的上升趋势;谷胱甘肽(GSH)和非蛋白巯基(NPT)含量在不同的镉浓度和处理时期都有增加的趋势,而植物络合素(PCs)则随着镉浓度的增加会出现先升高后趋于平稳的情况。甘蔗生理不仅受镉浓度处理的影响,随着时间的推移响应的镉积累也是左右甘蔗生理变化的重要因素。
In order to understand the physiological and biochemical responses of sugarcane under cadmium stress, barrel experiments were conducted to simulate the growth of sugarcane under cadmium stress, and to explore the effects of different concentrations and different periods of cadmium stress on the physiological and biochemical characteristics of sugarcane. The results showed that cadmium treatment had different effects on the chlorophyll content of sugarcane, and the change of chlorophyll content was more obvious with the passage of time. For scavenging enzymes of active oxygen system, including superoxide dismutase(SOD) and peroxidase(POD), there was a large fluctuation compared with the control. The contents of proline(PRO) and malondialdehyde(MDA) were less affected by the concentration, and did not change significantly in the early stage of cadmium treatment, but showed an obvious upward trend in the middle and late stage of treatment. The contents of glutathione(GSH) and non-protein thiol(NPT) tended to increase at different cadmium concentrations and treatment periods, while plant complexes(PCs) tended to increase first and then stabilize with the increase of cadmium concentration. Sugarcane physiology is not only affected by cadmium concentration, but also affected by cadmium accumulation over time.
In order to understand the physiological and biochemical responses of sugarcane under cadmium stress, barrel experiments were conducted to simulate the growth of sugarcane under cadmium stress, and to explore the effects of different concentrations and different periods of cadmium stress on the physiological and biochemical characteristics of sugarcane. The results showed that cadmium treatment had different effects on the chlorophyll content of sugarcane, and the change of chlorophyll content was more obvious with the passage of time. For scavenging enzymes of active oxygen system, including superoxide dismutase(SOD) and peroxidase(POD), there was a large fluctuation compared with the control. The contents of proline(PRO) and malondialdehyde(MDA) were less affected by the concentration, and did not change significantly in the early stage of cadmium treatment, but showed an obvious upward trend in the middle and late stage of treatment. The contents of glutathione(GSH) and non-protein thiol(NPT) tended to increase at different cadmium concentrations and treatment periods, while plant complexes(PCs) tended to increase first and then stabilize with the increase of cadmium concentration. Sugarcane physiology is not only affected by cadmium concentration, but also affected by cadmium accumulation over time.
引文
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[15]王朋,邓小娟,黄益安,等.镉胁迫下不同大豆品种各器官中镉和非蛋白巯基物质的动态变化[J].华南农业大学学报,2016,37(2):42-50.
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[25]夏会龙,程文伟,池小雅,等.镉胁迫对甘蔗生长及生理性状的影响[J].中国土壤与肥料,2009(1):42-45.
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[27]董姬妃,张帆,胡雨寒,等.镉胁迫下增施氮对白三叶草生长的影响和镉毒害的缓解效应研究[J].草业学报,2017,26(9):83-91.
[28]张金彪,黄维南.镉对植物的生理生态效应的研究进展[J].生态学报,2000,20(3):514-523.
[29]曹莹,李建东,赵天宏,等.镉胁迫对玉米生理生化特性的影响[J].农业环境科学学报,2007,26(增刊):8-11.
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[32]Pompella A,Visvikis A,Paolicchi A,et al.The changing faces of glutathione,a cellular protagonist[J].Biochemical Pharmacology,2003,66(8):1499-1503.
[33]刘俊祥,许新桥,钱永强,等.多年生黑麦草抗氧化酶和植物络合素对Cd2+胁迫的应答[J].生态学杂志,2013,32(7):1787-1793.
[34]高可辉,葛滢,张春华,等.缺硫对镉胁迫下水稻幼苗非蛋白巯基物质含量和谷胱甘肽硫转移酶活性的影响[J].应用生态学报,2011,22(7):1796-1802.
[35]欧阳燕莎,刘爱玉,李瑞莲,等.高低镉积累棉花品种生理生化特性研究[J].华北农学报,2017,32(6):170-174.
[2]欧阳燕莎,刘爱玉,李瑞莲,等.镉对作物的影响及作物对镉毒害响应研究进展[J].作物研究,2016,30(1):105-110.
[3]曾巧英,凌秋平,樊丽娜,等.镉胁迫对不同品种甘蔗生长及各器官镉积累的影响[J].热带作物学报,2016,37(11):2071-2076.
[4]孟自力,贾斌,尹海燕,等.镉胁迫对小麦生长发育的影响[J].中国农学通报,2018,34(23):26-32.
[5]余国莹,吴玉树,王焕校.不同化合形态镉、锌及其复合污染对小麦生理的影响[J].生态学报,1992,12(1):93-96.
[6]何俊瑜,任艳芳,朱诚期,等.镉胁迫对镉敏感水稻突变体活性氧代谢及抗氧化酶活性的影响[J].生态环境,2008,17(3):1004-1008.
[7]杨居荣,贺建群,蒋婉茹.Cd污染对植物生理生化的影响[J].农业环境保护,1995,14(5):193-197.
[8]黄玉山,罗广华,关棨文.镉诱导植物的自由基过氧化损伤[J].植物学报,1997,39(6):522-526.
[9]洪仁远,杨广笑,刘东华,等.镉对小麦幼苗的生长和生理生化反应的影响[J].华北农学报,1991,6(3):70-75.
[10]秦天才,吴玉树,王焕校.镉、铅及其相互作用对小白菜生理生化特性的影响[J].生态学报,1994,14(1):46-50.
[11]Scarano G,Morelli E.Characterization of cadmium-and leadphytochelatin complexes formed in a marine microalga in response to metal exposure[J].Biometals,2002,15(2):145-151.
[12]高华,张玉秀,柴团耀.植物络合素和植物络合素合酶的研究[J].西北植物学报,2001,21(4):779-790.
[13]赵迪,夏立江,朱永官,等.植物络合素的测定方法[J].生态学杂志,2005,24(3):330-334.
[14]李江遐,张军,马友华,等.不同水稻品种对镉的吸收转运及其非蛋白巯基含量的变化[J].生态环境学报,2017,26(12):2140-2145.
[15]王朋,邓小娟,黄益安,等.镉胁迫下不同大豆品种各器官中镉和非蛋白巯基物质的动态变化[J].华南农业大学学报,2016,37(2):42-50.
[16]杨振德.分光光度法测定叶绿素含量的探讨[J].广西农业大学学报,1996,15(2):145-150.
[17]Giannopolitis C N,Ries S K.Superoxide dismutases:I.occurrence in higher plants[J].Plant Physiology,1977,59(2):309-314.
[18]Sakharov I Y,Ardila G B.Variations of peroxidase activity in cocoa(Theobroma cacao L.)beans during their ripening,fermentation and drying[J].Food Chemistry,1999,65(1):51-54.
[19]Ohkawa H,Ohishi N,Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction[J].Analytical Biochemistry,1979,95(2):351-358.
[20]Bates L S,Waldren R P,Teare I D.Rapid determination of free proline for water-stress studies[J].Plant and Soil,1973,39(1):205-207.
[21]Bailly C,Kranner I.Analyses of reactive oxygen species and antioxidants in relation to seed longevity and germination[M].Bailly C,Kranner I.eds.Seed Dormancy.Totowa,NJ:Humana Press,2011.343-367.
[22]Ju X H,Tang S R,Jia Y,et al.Determination and characterization of cysteine,glutathione and phytochelatins(PC2-6)in Lolium perenne L.exposed to Cd stress under ambient and elevated carbon dioxide using HPLC with fluorescence detection[J].Journal of Chromatography B,2011,879(20):1717-1724.
[23]Thiebeauld O,Soler S,Raigón M D,et al.Variation among Solanaceae crops in cadmium tolerance and accumulation[J].Agronomy for Sustainable Development,2005,25(2):237-241.
[24]DalCorso G,Farinati S,Maistri S,et al.How plants cope with cadmium:Staking all on metabolism and gene expression[J].Journal of Integrative Plant Biology,2008,50(10):1268-1280.
[25]夏会龙,程文伟,池小雅,等.镉胁迫对甘蔗生长及生理性状的影响[J].中国土壤与肥料,2009(1):42-45.
[26]周宏,项斯端.重金属铜、锌、铅、镉对小形月牙藻生长及亚显微结构的影响[J].杭州大学学报(自然科学版),1998,25(2):85-92.
[27]董姬妃,张帆,胡雨寒,等.镉胁迫下增施氮对白三叶草生长的影响和镉毒害的缓解效应研究[J].草业学报,2017,26(9):83-91.
[28]张金彪,黄维南.镉对植物的生理生态效应的研究进展[J].生态学报,2000,20(3):514-523.
[29]曹莹,李建东,赵天宏,等.镉胁迫对玉米生理生化特性的影响[J].农业环境科学学报,2007,26(增刊):8-11.
[30]McGirr L G,O’Brien P J.Mechanisms of membrane lipid peroxidation[M].McGirr L G,O’Brien P J.eds.Recent Advances in Biological Membrane Studies.Boston,MA:Springer US,1985.319-344.
[31]袁祖丽,吴中红,刘秀敏,等.镉胁迫对烤烟叶片抗氧化系统的影响[J].河南农业科学,2008,37(7):43-46.
[32]Pompella A,Visvikis A,Paolicchi A,et al.The changing faces of glutathione,a cellular protagonist[J].Biochemical Pharmacology,2003,66(8):1499-1503.
[33]刘俊祥,许新桥,钱永强,等.多年生黑麦草抗氧化酶和植物络合素对Cd2+胁迫的应答[J].生态学杂志,2013,32(7):1787-1793.
[34]高可辉,葛滢,张春华,等.缺硫对镉胁迫下水稻幼苗非蛋白巯基物质含量和谷胱甘肽硫转移酶活性的影响[J].应用生态学报,2011,22(7):1796-1802.
[35]欧阳燕莎,刘爱玉,李瑞莲,等.高低镉积累棉花品种生理生化特性研究[J].华北农学报,2017,32(6):170-174.