CD166在含牙囊肿、牙源性角化囊性瘤及成釉细胞瘤中的表达及意义
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摘要
目的分析CD166在含牙囊肿(DC)、牙源性角化囊性瘤(KCOT)及成釉细胞瘤(SA)中的表达情况及其意义。方法选取71例经甲醛固定的标本,包括含牙囊肿14例、牙源性角化囊性瘤15例、成釉细胞瘤(AB)42例,其中实性成釉细胞瘤(SA)13例、单囊型成釉细胞瘤(UA)15例、外周型成釉细胞瘤(PA)14例。采用免疫组织化学染色的方法分析CD166在每种类型病变组织内的表达情况。结果 CD166的表达率分别为DC:0、KCOT:73.33%、SA:69.23%、UA:73.33%、PA:71.43%。含牙囊肿中CD166且表达部分基底膜上方的柱状细胞。结论 CD166在KCOT及AB中均呈现高表达,可能与其发生发展过程相关。
Objective To analysis the expression of CD166 and its meaning in dentigreous cyst,keraocystic odontogenic tumor and ameloblastoma. Methods 14 DC,15 KCOT and 42 AB( 13 SA,15 UA,14 PA) were reviewed by immunohistochemistry for CD166 staining. Results The expression rate of CD166 in each group,DC: 0,KCOT:73. 33%,SA: 69. 23%,UA: 73. 33%,PA: 71. 43%. DC had a significant lower expression compared with other groups. CD166 always expressed in membrane and/or cytoplasm of most of the cells in the basal and suprabasal layers. Conclusion CD166 has high expression in KCOT and AB,which has a role in the pathogenesis of AB and KCOT.
Objective To analysis the expression of CD166 and its meaning in dentigreous cyst,keraocystic odontogenic tumor and ameloblastoma. Methods 14 DC,15 KCOT and 42 AB( 13 SA,15 UA,14 PA) were reviewed by immunohistochemistry for CD166 staining. Results The expression rate of CD166 in each group,DC: 0,KCOT:73. 33%,SA: 69. 23%,UA: 73. 33%,PA: 71. 43%. DC had a significant lower expression compared with other groups. CD166 always expressed in membrane and/or cytoplasm of most of the cells in the basal and suprabasal layers. Conclusion CD166 has high expression in KCOT and AB,which has a role in the pathogenesis of AB and KCOT.
引文
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[11]Kaur J,Sawhney M,Datta Gupta S,et al.Clinical significance of altered expression ofβ-catenin and E-cadherin in oral dysplasia and cancer:potential link with ALCAM expression[J].PLo S One,2013,8(6):e67361.
[12]González-Alva P,Tanaka A,Oku Y,et al.Enhanced expression of podoplanin in ameloblastomas[J].J Oral Pathol Med,2010,39(9):103-109.
[13]van den Brand M,Takes RP,Blokpoel-deRuyter M,et al.Activated leukocyte cell adhesion molecule expression predicts lymph node metastasis in oral squamous cell carcinoma[J].Oral Oncol,2010,46(5):393-398.
[14]Lunter PC,van Kilsdonk JW,van Beek H,et al.Activated leukocyte cell adhesion molecule(ALCAM/CD166/MEMD),a novel actor in invasive growth,controls matrix metalloproteinase activity[J].Cancer Res,2005,65(19):8801-8808.
[15]Khalifa GA,Shokier HM,Abo-Hager EA.Evaluation of neoplastic nature of keratocystic odontogenic tumor versus ameloblastoma[J].J Egypt Natl Canc Inst,2010,22(1):61-72.
[2]Alves Pereira KM,do Amaral BA,dos Santos BR,et al.Immunohistochemical expression of E-cadherin and beta-catenin in ameloblastomas and tooth germs[J].Oral Surg Oral Med Oral Pathol Oral Radiol Endod,2010,109(1):425-431.
[3]Weidle UH,Eggle D,Klostermann S,et al.ALCAM/CD166:cancer-related issues[J].Cancer Genomics Prot,2010,7(5):231-243.
[4]Tuyns AJ,Estève J,Raymond L,et al.Cancer of the larynx/hypopharynx,tobacco and alcohol:IARC international casecontrol study in Turin and Varese(Italy),Zaragoza and Navarra(Spain),Geneva(Switzerland)and Calvados(France)[J].Int J Cancer,1988,41(5):483-491.
[5]Yan Ming,Yang Xihu,Wang Lizhen,et al.Plasma membrane proteomics of tumor spheres identify CD166 as a novel marker for cancer stem-like cells in head and neck squamous cell carcinoma[J].Mol Cell Proteomics,2013,12(6):3271-3284.
[6]Goloudina AR,Demidov ON,Garrido C.Inhibition of HSP70:a challenging anti-cancer strategy[J].Cancer Lett,2012,325(9):117-124.
[7]Salehinejad J,Saghafi S,Sharifi N,et al.Evaluation of osteopontin and CD166 expression in odontogenic cystic lesions by immunohistochemistry[J].Pathol Res Pract,2012,208(7):410-414.
[8]Rosso O,Piazza T,Bongarzone I,et al.The ALCAM shedding by the metalloprotease ADAM17/TACE is involved in motility of ovarian carcinoma cells[J].Mol Cancer Res,2007,5(12):1246-1253.
[9]Shimada Y,Katsube K,Kabasawa Y,et al.Integrated genotypic analysis of hedgehog-related genes identifies subgroups of keratocystic odontogenic tumor with distinct clinicopathological features[J].PLo S One,2013,8(8):e70995.
[10]Hansen AG,Arnold SA,Jiang Ming,et al.ALCAM/CD166is a TGF-β-responsive marker and functional regulator of prostate cancer metastasis to bone[J].Cancer Res,2014,74(5):1404-1415.
[11]Kaur J,Sawhney M,Datta Gupta S,et al.Clinical significance of altered expression ofβ-catenin and E-cadherin in oral dysplasia and cancer:potential link with ALCAM expression[J].PLo S One,2013,8(6):e67361.
[12]González-Alva P,Tanaka A,Oku Y,et al.Enhanced expression of podoplanin in ameloblastomas[J].J Oral Pathol Med,2010,39(9):103-109.
[13]van den Brand M,Takes RP,Blokpoel-deRuyter M,et al.Activated leukocyte cell adhesion molecule expression predicts lymph node metastasis in oral squamous cell carcinoma[J].Oral Oncol,2010,46(5):393-398.
[14]Lunter PC,van Kilsdonk JW,van Beek H,et al.Activated leukocyte cell adhesion molecule(ALCAM/CD166/MEMD),a novel actor in invasive growth,controls matrix metalloproteinase activity[J].Cancer Res,2005,65(19):8801-8808.
[15]Khalifa GA,Shokier HM,Abo-Hager EA.Evaluation of neoplastic nature of keratocystic odontogenic tumor versus ameloblastoma[J].J Egypt Natl Canc Inst,2010,22(1):61-72.