肉苁蓉不同提取部位改善肾阳虚大鼠性能力的影响
|
摘要
目的:观察肉苁蓉不同提取部位对氢化可的松致肾阳虚模型大鼠性能力的影响。方法:将50只雄性SD大鼠随机分为正常组、阳虚模型组、肉苁蓉总寡糖组、肉苁蓉总多糖组、肉苁蓉苯乙醇总苷组。连续14 d肌肉注射氢化可的松制备肾阳虚动物模型,给药组按肉苁蓉生药量2.3 g·kg-1对应给予肉苁蓉总寡糖103.82 mg·kg-1,肉苁蓉总多糖115.64 mg·kg-1,肉苁蓉苯乙醇总苷201.48 mg·kg-1水溶液,每日1次。14 d后测定各组大鼠的勃起潜伏期,交配能力各指标(包括捕捉潜伏期、捕捉次数、射精潜伏期、射精次数、捕捉率、射精率等),脏器湿重及精浆果糖含量,血浆环磷酸腺苷(c AMP)/环磷酸乌苷(c GMP)值,血清激素睾酮(T),雌二醇(E2),促卵泡生成素(FSH),促黄体生成素(LH)及促性腺激素释放激素(GnRH)水平,观察睾丸病理形态变化。结果:肉苁蓉不同提取部位对肾阳虚大鼠的勃起功能、交配能力、脏器湿重及精浆果糖含量,血浆c AMP/c GMP值,血清激素水平,睾丸病理形态变化均有作用。其中肉苁蓉苯乙醇总苷提取物能够显著缩短大鼠的勃起潜伏期(P<0.05)及捕捉潜伏期(P<0.01),显著增加捕捉次数(P<0.01),睾丸(P<0.05),精囊腺(P<0.01)湿重及精浆果糖含量(P<0.05),显著升高血浆c AMP/c GMP值(P<0.01)及血清T(P<0.01),FHS(P<0.01),GnRH(P<0.05)水平,显著降低血清E2(P<0.05),LH(P<0.05)水平,显著改善睾丸病理形态。结论:肉苁蓉不同提取部位可通过调控氢化可的松致肾阳虚大鼠模型的激素水平不同程度提高其性能力,其中苯乙醇总苷可显著改善肾阳虚大鼠性能力相关指标。
Objective: To observe the effect of extracts from on the sexual performance of rats with kidney-Yang deficiency induced by hydrocortisone. Method: Fifty male SD rats were randomly divided into normal control group,kidney-Yang deficiency model group,Cistanches Herba total oligosaccharide group,Cistanches Herba total polysaccharide group,and Cistanches Herba total phenylethanol glycosides,with 10 in each group. The animal model of kidney-Yang deficiency was prepared through intramuscular injection with hydrocortisone for 14 consecutive days. The medication groups were given aqueous solutions of total oligosaccharides of Cistanches Herba(103. 28 mg ·kg-1),total polysaccharides of Cistanches Herba(115. 64 mg·kg-1),and total glycosides of phenylethanol of Cistanches Herba(201. 48 mg·kg-1),according to the dose of 2. 3 g·kg-1 of Cistanches Herba raw material once a day. After 14 days,erectile latency,indexes of mating ability(including capture latency,the number of captures,the ejaculation latency,the number of ejaculations),organ wet weight and berry sugar content,plasma cyclic adenosine monophosphate(c AMP)/cyclic guanosine monophosphate(c GMP) ratio,serum hormone testosterone(T),estradiol(E2),and follicle-stimulating hormone(FSH),luteinizing hormone(LH) and gonadotropin-releasing hormone(GnRH) levels were determined in each group of rats,and the testicular morphological changes were observed. Result: The extracts from different fractions of Cistanches Herba had the effect on erectile function,mating ability,organ wet weight and berry sugar content,plasma c AMP/c GMP value,serum hormone level,and testicular pathological changes in rats with kidney-Yang deficiency. Particularly,the extracts of total phenylethanol glycosides had the best effect,and could significantly reduce the erectile latency(P < 0. 05) and capture latency(P < 0. 01),significantly increase the times of capture(P < 0. 01),testis(P <0. 05),seminal vesicles(P < 0. 01) wet weight and berry sugar content(P < 0. 05),significantly increase plasma c AMP/c GMP values(P < 0. 01),serum T(P < 0. 01),FHS(P < 0. 01),GnRH(P < 0. 05) levels,significantly reduce serum E2(P < 0. 05),LH(P < 0. 05) levels,and significantly improve pathological morphology of testis in rats. Conclusion: Extracts from different fractions of Cistanches Herba can regulate hormone levels in rats with kidney-Yang deficiency induced by hydrocortisone and further improve their sexual ability. The total glycosides of phenylethanol can significantly improve the indexes related to sexual performance in rats with kidney-Yang deficiency.
Objective: To observe the effect of extracts from on the sexual performance of rats with kidney-Yang deficiency induced by hydrocortisone. Method: Fifty male SD rats were randomly divided into normal control group,kidney-Yang deficiency model group,Cistanches Herba total oligosaccharide group,Cistanches Herba total polysaccharide group,and Cistanches Herba total phenylethanol glycosides,with 10 in each group. The animal model of kidney-Yang deficiency was prepared through intramuscular injection with hydrocortisone for 14 consecutive days. The medication groups were given aqueous solutions of total oligosaccharides of Cistanches Herba(103. 28 mg ·kg-1),total polysaccharides of Cistanches Herba(115. 64 mg·kg-1),and total glycosides of phenylethanol of Cistanches Herba(201. 48 mg·kg-1),according to the dose of 2. 3 g·kg-1 of Cistanches Herba raw material once a day. After 14 days,erectile latency,indexes of mating ability(including capture latency,the number of captures,the ejaculation latency,the number of ejaculations),organ wet weight and berry sugar content,plasma cyclic adenosine monophosphate(c AMP)/cyclic guanosine monophosphate(c GMP) ratio,serum hormone testosterone(T),estradiol(E2),and follicle-stimulating hormone(FSH),luteinizing hormone(LH) and gonadotropin-releasing hormone(GnRH) levels were determined in each group of rats,and the testicular morphological changes were observed. Result: The extracts from different fractions of Cistanches Herba had the effect on erectile function,mating ability,organ wet weight and berry sugar content,plasma c AMP/c GMP value,serum hormone level,and testicular pathological changes in rats with kidney-Yang deficiency. Particularly,the extracts of total phenylethanol glycosides had the best effect,and could significantly reduce the erectile latency(P < 0. 05) and capture latency(P < 0. 01),significantly increase the times of capture(P < 0. 01),testis(P <0. 05),seminal vesicles(P < 0. 01) wet weight and berry sugar content(P < 0. 05),significantly increase plasma c AMP/c GMP values(P < 0. 01),serum T(P < 0. 01),FHS(P < 0. 01),GnRH(P < 0. 05) levels,significantly reduce serum E2(P < 0. 05),LH(P < 0. 05) levels,and significantly improve pathological morphology of testis in rats. Conclusion: Extracts from different fractions of Cistanches Herba can regulate hormone levels in rats with kidney-Yang deficiency induced by hydrocortisone and further improve their sexual ability. The total glycosides of phenylethanol can significantly improve the indexes related to sexual performance in rats with kidney-Yang deficiency.
引文
[1]雷载权.中药学[M].上海:上海科学技术出版社,1995:286.
[2]于宏波.阳痿肾阳虚证症状规律及其转录组特征研究[D].成都:成都中医药大学,2011.
[3]沈自尹.肾阳虚证的定位研究[J].中国中西医结合杂志,1997,17(1):50-52.
[4]李炳如,佘运初.补肾药对下丘脑-垂体-性腺轴功能影响[J].中医杂志,1984,30(7):63-65.
[5]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:135-136.
[6]吴波,顾少菊,傅玉梅,等.肉苁蓉和管花肉苁蓉通便与补肾壮阳药理作用的研究[J].中医药学刊,2003,21(4):539-548.
[7]王德俊,盛树青,梁虹.肉苁蓉对小鼠睾丸和附睾形态学及组织化学的影响[J].解剖学研究,2000,22(2):101-103.
[8]李福香,徐敏,王键,等.肉苁蓉的药理学研究现状与展望[J].安徽农学通报,2006,12(13):92-93,70.
[9]张子怡,陈宝军,张庆,等.肾阳虚证动物模型的造模方法与评价指标[J].福建中医药,2014,45(2):61-63.
[10]王琦,倪平,吴卫平,等.疏肝益阳胶囊治疗勃起功能障碍的作用机理研究[J].中国中药杂志,2005,30(1):58-63.
[11]田二坡,龙廷,秦达念.雄性大鼠交配实验模型的建立[J].中国男科学杂志,2008,22(1):7-10.
[12]肖静,王毅兴,高建东,等.肾阳虚证的研究进展[J].上海中医药大学学报,2008,22(2):73-77.
[13]罗琼,黄晓兰,李卓能,等.枸杞多糖对雄性大鼠性功能及生殖功能的影响[J].营养学报,2006,28(1):62-65,70.
[14]喻小明.脾气虚对雄性大鼠性功能影响的实验研究[D].武汉:湖北中医学院,2009.
[15]李妍,侯丽辉,吴效科.中医肾主生殖理论探讨及现代研究进展[J].世界中西医结合杂志,2008,3(9):557-559.
[16]陈英华,欧阳轶强,孙琪,等.肾阳虚证动物模型规范化研究中诊断指标选择的初步探讨[J].中国中医基础医学杂志,2003,9(10):26-30.
[17]曹素梅,万雪萍,严美姣,等.miRNAs介导下丘脑-垂体-性腺轴调控动物生殖的研究进展[J].中国畜牧杂志,2017,53(1):1-6.
[18]马淑然,刘燕池,郭霞珍.“肾主生殖”理论整理及实验研究概况(综述)[J].北京中医药大学学报,2000(Z1):49-52.
[19]伍庆华,王建红,祁风义.肾阳虚与下丘脑-垂体-靶腺轴研究概述[J].江西中医学院学报,2006,18(6):73-74.
[20]高云佳,姜勇,戴昉,等.肉苁蓉润肠通便的药效物质研究[J].中国现代中药,2015,17(4):307-310,314.
[21]马慧,尹若熙,郭敏,等.肉苁蓉多糖对D-半乳糖致衰老模型小鼠CREB表达的影响[J].中国实验方剂学杂志,2014,20(20):137-141.
[22]高晓霞,陈君,彭艳丽.肉苁蓉多糖药理作用研究概况[J].食品与药品,2015,17(2):136-139.
[23]薛海燕,焦婵媛,姚军.肉苁蓉总苷药理作用的研究现状[J].中国临床药理学杂志,2018,34(4):486-488.
[24]颜贵卉,田金虎,龙本文,等.肉苁蓉中苯乙醇苷类成分的研究进展[J].中南药学,2012,10(9):692-695.
[25]南泽东,任华忠,赵明波,等.HPLC-MS鉴定塔中栽培荒漠肉苁蓉药材的17个成分[J].中国实验方剂学杂志,2018,24(16):76-81.
[26]王德俊,陈飞,张洪泉,等.麦角甾苷增强大鼠性功能的实验研究[C]∥第四届肉苁蓉暨沙生药用植物学术研讨会论文集,2007:107-112.
[27]JIANG Z H,WANG J,ZHANG X P,et al.Echinacoside and Cistanche tubulosa(Schenk)R.wight ameliorate bisphenol Ainduced testicular and sperm damage in rats through gonad axis regulated steroid ogenic enzymes[J].J Ethnopharmacol,2016,193:321-328.
[28]赵东海,张磊,张艳,等.肉苁蓉苯乙醇苷对环磷酰胺致小鼠生精障碍的治疗作用及其机制[J].吉林大学学报:医学版,2014,40(3):612-615.
[29]李刚,朱文斌,牛飞,等.肉苁蓉苯乙醇苷对大鼠精子体外氧化损伤的保护作用研究[J].时珍国医国药,2010,21(9):2205-2207.
[30]徐启华,胡明华,马方励,等.虚证动物模型的研究现状[J].中国实验方剂学杂志,2012,18(11):307-312.
[2]于宏波.阳痿肾阳虚证症状规律及其转录组特征研究[D].成都:成都中医药大学,2011.
[3]沈自尹.肾阳虚证的定位研究[J].中国中西医结合杂志,1997,17(1):50-52.
[4]李炳如,佘运初.补肾药对下丘脑-垂体-性腺轴功能影响[J].中医杂志,1984,30(7):63-65.
[5]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:135-136.
[6]吴波,顾少菊,傅玉梅,等.肉苁蓉和管花肉苁蓉通便与补肾壮阳药理作用的研究[J].中医药学刊,2003,21(4):539-548.
[7]王德俊,盛树青,梁虹.肉苁蓉对小鼠睾丸和附睾形态学及组织化学的影响[J].解剖学研究,2000,22(2):101-103.
[8]李福香,徐敏,王键,等.肉苁蓉的药理学研究现状与展望[J].安徽农学通报,2006,12(13):92-93,70.
[9]张子怡,陈宝军,张庆,等.肾阳虚证动物模型的造模方法与评价指标[J].福建中医药,2014,45(2):61-63.
[10]王琦,倪平,吴卫平,等.疏肝益阳胶囊治疗勃起功能障碍的作用机理研究[J].中国中药杂志,2005,30(1):58-63.
[11]田二坡,龙廷,秦达念.雄性大鼠交配实验模型的建立[J].中国男科学杂志,2008,22(1):7-10.
[12]肖静,王毅兴,高建东,等.肾阳虚证的研究进展[J].上海中医药大学学报,2008,22(2):73-77.
[13]罗琼,黄晓兰,李卓能,等.枸杞多糖对雄性大鼠性功能及生殖功能的影响[J].营养学报,2006,28(1):62-65,70.
[14]喻小明.脾气虚对雄性大鼠性功能影响的实验研究[D].武汉:湖北中医学院,2009.
[15]李妍,侯丽辉,吴效科.中医肾主生殖理论探讨及现代研究进展[J].世界中西医结合杂志,2008,3(9):557-559.
[16]陈英华,欧阳轶强,孙琪,等.肾阳虚证动物模型规范化研究中诊断指标选择的初步探讨[J].中国中医基础医学杂志,2003,9(10):26-30.
[17]曹素梅,万雪萍,严美姣,等.miRNAs介导下丘脑-垂体-性腺轴调控动物生殖的研究进展[J].中国畜牧杂志,2017,53(1):1-6.
[18]马淑然,刘燕池,郭霞珍.“肾主生殖”理论整理及实验研究概况(综述)[J].北京中医药大学学报,2000(Z1):49-52.
[19]伍庆华,王建红,祁风义.肾阳虚与下丘脑-垂体-靶腺轴研究概述[J].江西中医学院学报,2006,18(6):73-74.
[20]高云佳,姜勇,戴昉,等.肉苁蓉润肠通便的药效物质研究[J].中国现代中药,2015,17(4):307-310,314.
[21]马慧,尹若熙,郭敏,等.肉苁蓉多糖对D-半乳糖致衰老模型小鼠CREB表达的影响[J].中国实验方剂学杂志,2014,20(20):137-141.
[22]高晓霞,陈君,彭艳丽.肉苁蓉多糖药理作用研究概况[J].食品与药品,2015,17(2):136-139.
[23]薛海燕,焦婵媛,姚军.肉苁蓉总苷药理作用的研究现状[J].中国临床药理学杂志,2018,34(4):486-488.
[24]颜贵卉,田金虎,龙本文,等.肉苁蓉中苯乙醇苷类成分的研究进展[J].中南药学,2012,10(9):692-695.
[25]南泽东,任华忠,赵明波,等.HPLC-MS鉴定塔中栽培荒漠肉苁蓉药材的17个成分[J].中国实验方剂学杂志,2018,24(16):76-81.
[26]王德俊,陈飞,张洪泉,等.麦角甾苷增强大鼠性功能的实验研究[C]∥第四届肉苁蓉暨沙生药用植物学术研讨会论文集,2007:107-112.
[27]JIANG Z H,WANG J,ZHANG X P,et al.Echinacoside and Cistanche tubulosa(Schenk)R.wight ameliorate bisphenol Ainduced testicular and sperm damage in rats through gonad axis regulated steroid ogenic enzymes[J].J Ethnopharmacol,2016,193:321-328.
[28]赵东海,张磊,张艳,等.肉苁蓉苯乙醇苷对环磷酰胺致小鼠生精障碍的治疗作用及其机制[J].吉林大学学报:医学版,2014,40(3):612-615.
[29]李刚,朱文斌,牛飞,等.肉苁蓉苯乙醇苷对大鼠精子体外氧化损伤的保护作用研究[J].时珍国医国药,2010,21(9):2205-2207.
[30]徐启华,胡明华,马方励,等.虚证动物模型的研究现状[J].中国实验方剂学杂志,2012,18(11):307-312.