耳针对血管性痴呆大鼠认知功能及海马神经细胞的影响
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摘要
目的动态观察耳针对血管性痴呆(vascular dementia,VD)大鼠认知功能及海马神经细胞的影响。方法从60只健康雄性SD大鼠中随机选取10只为正常组,其余采用四血管阻断法复制VD大鼠模型,然后随机分为模型组、耳针组、西药组,每组10只。于模型复制后第4天,治疗第30、60天时,采用跳台实验检测各组大鼠的学习记忆成绩,并进行神经行为学评分;于模型复制后第4天、治疗第60天时,光镜下观察各组大鼠海马CA1区神经细胞形态。结果模型复制后第4天,与正常组比较,模型组大鼠学习记忆成绩、神经行为学评分及海马CA1区锥体存活细胞数差异均有统计学意义(P<0.05);耳针治疗30、60 d后,与模型组比较,耳针组及西药组大鼠学习记忆成绩、神经行为学评分差异均有统计学意义(P<0.05),耳针组与西药组比较,差异无统计学意义(P>0.05);治疗60 d后,耳针组、西药组大鼠海马CA1区锥体细胞存活数与模型组比较,差异均有统计学意义(P<0.05);耳针组与西药组相比,差异无统计学意义(P>0.05)。结论耳针可以提高VD大鼠的学习记忆能力,降低神经行为学评分;耳针可改善变性的海马CA1神经细胞,促进其修复与再生,从而提高VD大鼠学习记忆功能,这可能是临床耳针治疗VD有效作用的可能机制。
Objective To investigate the effect of ear acupuncture on cognitive function and hippocampal neurons in rats with vascular dementia(VD).Methods A total of 60 healthy male Sprague-Dawley rats were selected,among which 10 rats were enrolled as normal group and the remaining were treated with four-vessel occlusion to establish a model of VD and were then randomly divided into model group,auricular acupuncture group,and Western medicine group,with 10 rats in each group.On day 4 after modeling and on days 30 and 60 of treatment,the step-down test was performed to evaluate learning and memory performance and determine neurobehavioral score;on day 4 after modeling and on day 60 of treatment,the morphology of hippocampal CA1 neurons was observed under a light microscope.Results On day 4 after modeling,there were significant differences between the normal group and the model group in learning and memory performance,neurobehavioral score,and the number of viable pyramidal cells in the hippocampal CA1 region(P<0.05).On days 30 and 60 of ear acupuncture therapy,there were significant differences in learning and memory performance and neurobehavioral score between the auricular acupuncture/Western medicine groups and the model group(P<0.05),while there were no significant differences between theauricular acupuncture group and the Western medicine group(P>0.05).After 60 days of treatment,there were significant differences in these indices between the auricular acupuncture/Western medicine groups and the model group(P<0.05),while there were no significant differences between the auricular acupuncture group and the Western medicine group(P>0.05).Conclusion Auricular acupuncture can improve the learning and memory ability of VD rats and reduce their neurobehavioral score.It can also improve the degenerative hippocampal CA1 neurons,promote their recovery and regeneration,and thus improve the learning and memory ability of VD rats,which might be the possible mechanism of auricular acupuncture in the treatment of VD in clinical practice.
Objective To investigate the effect of ear acupuncture on cognitive function and hippocampal neurons in rats with vascular dementia(VD).Methods A total of 60 healthy male Sprague-Dawley rats were selected,among which 10 rats were enrolled as normal group and the remaining were treated with four-vessel occlusion to establish a model of VD and were then randomly divided into model group,auricular acupuncture group,and Western medicine group,with 10 rats in each group.On day 4 after modeling and on days 30 and 60 of treatment,the step-down test was performed to evaluate learning and memory performance and determine neurobehavioral score;on day 4 after modeling and on day 60 of treatment,the morphology of hippocampal CA1 neurons was observed under a light microscope.Results On day 4 after modeling,there were significant differences between the normal group and the model group in learning and memory performance,neurobehavioral score,and the number of viable pyramidal cells in the hippocampal CA1 region(P<0.05).On days 30 and 60 of ear acupuncture therapy,there were significant differences in learning and memory performance and neurobehavioral score between the auricular acupuncture/Western medicine groups and the model group(P<0.05),while there were no significant differences between theauricular acupuncture group and the Western medicine group(P>0.05).After 60 days of treatment,there were significant differences in these indices between the auricular acupuncture/Western medicine groups and the model group(P<0.05),while there were no significant differences between the auricular acupuncture group and the Western medicine group(P>0.05).Conclusion Auricular acupuncture can improve the learning and memory ability of VD rats and reduce their neurobehavioral score.It can also improve the degenerative hippocampal CA1 neurons,promote their recovery and regeneration,and thus improve the learning and memory ability of VD rats,which might be the possible mechanism of auricular acupuncture in the treatment of VD in clinical practice.
引文
[1] 翁虹,黄坚红.血管性痴呆的定义及诊断进展[J].广东医学,2010,31(14):1881-1882.
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[10] 孟丽梅.脑小血管病患者伴发非痴呆血管性认知功能损害情况及尼莫地平疗效的分析[J].中国医药指南,2018(33):85-86.
[11] 刘晓庆.尼莫地平治疗脑出血后缺血性脑损伤的临床观察[J].中国处方药,2018,16(11):90-91.
[12] TORT A B L,KOMOROWSKI R W,MANNS J R,et al.Theta-gamma coupling increases during the learning of item-context associations[J].Proc Natl Acad Sci USA,2009,106(49):20942-20947.
[13] NIKONENKO A G,RADENOVIC L,ANDJUS P R,et al.Structural features of ischemic damage in the hippocampus[J].Anat Rec,2009,292(12):1914-1921.
[14] KERCHNER G A,HESS C P,HAMMONDROSENBLUTH K E,et al.Hippocampal CA1 apical neuropil atrophy in mild Alzheimer disease visualized with 7-T MRI (Podcast)[J].Neurology,2010,75(15):1381-1387.
[15] KERCHNER G A,DEUTSCH G K,ZEINEH M,et al.Hippocampal CA1 apical neuropil atrophy and memory performance in Alzheimer's disease[J].Neuroimage,2012,63(1):194-202.
[16] 唐中生,吕明庄,贺志光,等.耳针、中药改善血管性痴呆大鼠学习记忆障碍与海马AchE的关系[J].贵阳中医学院学报,2007(3):38-40.
[17] 吕杭州,王春庆,吕明庄,等.耳针对血管性痴呆大鼠海马神经元细胞凋亡的干预研究[J].河北医药,2009,31(14):1713-1715.
[18] 张雪朝,肖茂磊,孙国杰.耳针改善血管性痴呆大鼠记忆障碍及其与bcl-2表达的关系[J].针刺研究,2001(2):106-110.
[2] PULSINELLI W A,BRIERLEY J B.A new model of bilateral hemispheric ischemia in the anaesthetized rat[J].Stroke,1979,10(3):267-272.
[3] 华兴邦.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,11(6):1.
[4] 金杰,陈海燕,关明智,等.建脑益智液作用的实验研究[J].成都中医药大学报,1998,21(1):49.
[5] LONGA E Z,WEINSTEIN P R,CARLSON S,et al.Reversible middle cerebral artery occlusion without craniotomy in rats[J].Stroke,1989,20(1):84-91.
[6] 张雪朝,吕明庄,蒋乃昌,等.耳针对血管性痴呆大鼠记忆及海马凋亡相关蛋白表达的影响[J].中国针灸,2001,21(8):499.
[7] 陈峰,戴晴,杨易平,等.耳穴神门对脑动脉硬化症患者椎-基底动脉血即时作用观察[J].中国针灸,1997,17(12):717.
[8] 范丽.尼莫地平治疗急性缺血性卒中后轻度认知障碍的疗效观察[J].世界最新医学信息文摘,2018(97):83,85.
[9] 孟秀丽.尼莫地平治疗脑出血后缺血性脑损伤的临床效果观察[J].中国医药指南,2018(33):81-82.
[10] 孟丽梅.脑小血管病患者伴发非痴呆血管性认知功能损害情况及尼莫地平疗效的分析[J].中国医药指南,2018(33):85-86.
[11] 刘晓庆.尼莫地平治疗脑出血后缺血性脑损伤的临床观察[J].中国处方药,2018,16(11):90-91.
[12] TORT A B L,KOMOROWSKI R W,MANNS J R,et al.Theta-gamma coupling increases during the learning of item-context associations[J].Proc Natl Acad Sci USA,2009,106(49):20942-20947.
[13] NIKONENKO A G,RADENOVIC L,ANDJUS P R,et al.Structural features of ischemic damage in the hippocampus[J].Anat Rec,2009,292(12):1914-1921.
[14] KERCHNER G A,HESS C P,HAMMONDROSENBLUTH K E,et al.Hippocampal CA1 apical neuropil atrophy in mild Alzheimer disease visualized with 7-T MRI (Podcast)[J].Neurology,2010,75(15):1381-1387.
[15] KERCHNER G A,DEUTSCH G K,ZEINEH M,et al.Hippocampal CA1 apical neuropil atrophy and memory performance in Alzheimer's disease[J].Neuroimage,2012,63(1):194-202.
[16] 唐中生,吕明庄,贺志光,等.耳针、中药改善血管性痴呆大鼠学习记忆障碍与海马AchE的关系[J].贵阳中医学院学报,2007(3):38-40.
[17] 吕杭州,王春庆,吕明庄,等.耳针对血管性痴呆大鼠海马神经元细胞凋亡的干预研究[J].河北医药,2009,31(14):1713-1715.
[18] 张雪朝,肖茂磊,孙国杰.耳针改善血管性痴呆大鼠记忆障碍及其与bcl-2表达的关系[J].针刺研究,2001(2):106-110.