利用近红外光谱技术快速评定油菜秸秆的营养价值
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摘要
本研究旨在利用近红外光谱技术(near infrared reflectance spectroscopy, NIRS)建立油菜(Brassica napus)秸秆常规成分的近红外快速预测模型。从甘肃省、青海省与宁夏回族自治区共采集125份油菜秸秆,测定其干物质(dry matter,DM)、粗蛋白(crude protein, CP)、中性洗涤纤维(neutral detergent fiber, NDF)、酸性洗涤纤维(acid detergent fiber, ADF)、粗脂肪(ether extract, EE)、粗灰分(Ash)、中性洗涤不溶蛋白质(neutral detergent insoluble protein, NDIP)、酸性洗涤不溶蛋白质(acid detergent insoluble protein, ADIP)、酸性洗涤木质素(acid detergent lignin, ADL)、非蛋白氮(non-protein nitrogen,NPN)与可溶性蛋白(soluble protein, SP)。选取100份油菜秸秆样品作为定标集,另外25份作为验证集来评价NIRS预测模型。结果显示,1)油菜秸秆DM、CP、NDF、ADF、EE、Ash、NDIP、ADIP、ADL、NPN和SP的含量分别为93.62%、5.85%、 67.22%、 55.40%、 2.57%、 7.66%、 1.04%、 0.63%、 13.58%、 1.70%和2.89%。2)DM、 CP、 NDF、 EE、 Ash、NPN和SP的交互验证决定系数(1-VR)> 0.9,外部验证决定系数(RQS≥0.84),构建模型可以用于日常分析。3)DM、CP、NDF、EE、Ash、NPN和SP的模型参数分别为Standard MSC 1,4,4,1、Weighted MSC 2,4,4,1、SNV 2,4,4,1、Standard MSC 1,4,4,1、SNV and Detrend 1,4,4,1、Weighted MSC 1,4,4,1,其余营养成分构建模型不太理想,需进一步完善。综上,通过交互验证与外部验证验证了油菜秸秆各营养成分校正模型的可利用性。
The experiment aims to establish a near-infrared fast-prediction model which measures the nutrition components of rapeseed straw using near-infrared reflectance spectroscopy(NIRS). 125 rape straw samples were collected from Gansu Province, Qinghai Province and Ningxia Hui Autonomous Region, and then the dry matter(DM), crude protein(CP), neutral detergent fiber(NDF), acid detergent fiber(ADF), ether extract(EE), coarse ash(Ash), neutral detergent insoluble protein(NDIP), acidic detergent insoluble protein(ADIP), acid detergent lignin(ADL), non-protein nitrogen(NPN) and soluble protein(SP) of the samples were determined. 100 rape straw samples were selected as calibration set to build calibration models. Another 25 samples were used as external validation set to evaluate the NIRS prediction model. The results showed that: 1) the contents of DM, CP, NDF, ADF, EE, Ash, NDIP, ADIP, ADL and NPN in rape straw were 93.62%, 5.85%,67.22%, 55.40%, 2.57%, 7.66% and 1.04%, 0.63%, 13.58%, 1.70%, respectively. 2) The coefficient of determination for cross-validation(1-VR) and coefficient of determination for calibration of DM, CP, NDF, EE, Ash, NPN and SP were greater than 0.9 and 0.84, respectively, indicating that these NIRS models could be used in routine analysis. 3)The model parameters of DM, CP, NDF, EE, Ash, NPN and SP were Standard MSC 1,4,4,1, Weighted MSC 2,4,4,1, SNV 2,4,4,1, Standard MSC1,4,4,1, SNV and Detrend 1,4,4,1, Weighted MSC 1,4,4,1, respectively, whereas the model of other nutritional components is not ideal and needs further improvement. In summary, the availability of nutritional composition correction model for rape straw was verified by internal and external validation.
The experiment aims to establish a near-infrared fast-prediction model which measures the nutrition components of rapeseed straw using near-infrared reflectance spectroscopy(NIRS). 125 rape straw samples were collected from Gansu Province, Qinghai Province and Ningxia Hui Autonomous Region, and then the dry matter(DM), crude protein(CP), neutral detergent fiber(NDF), acid detergent fiber(ADF), ether extract(EE), coarse ash(Ash), neutral detergent insoluble protein(NDIP), acidic detergent insoluble protein(ADIP), acid detergent lignin(ADL), non-protein nitrogen(NPN) and soluble protein(SP) of the samples were determined. 100 rape straw samples were selected as calibration set to build calibration models. Another 25 samples were used as external validation set to evaluate the NIRS prediction model. The results showed that: 1) the contents of DM, CP, NDF, ADF, EE, Ash, NDIP, ADIP, ADL and NPN in rape straw were 93.62%, 5.85%,67.22%, 55.40%, 2.57%, 7.66% and 1.04%, 0.63%, 13.58%, 1.70%, respectively. 2) The coefficient of determination for cross-validation(1-VR) and coefficient of determination for calibration of DM, CP, NDF, EE, Ash, NPN and SP were greater than 0.9 and 0.84, respectively, indicating that these NIRS models could be used in routine analysis. 3)The model parameters of DM, CP, NDF, EE, Ash, NPN and SP were Standard MSC 1,4,4,1, Weighted MSC 2,4,4,1, SNV 2,4,4,1, Standard MSC1,4,4,1, SNV and Detrend 1,4,4,1, Weighted MSC 1,4,4,1, respectively, whereas the model of other nutritional components is not ideal and needs further improvement. In summary, the availability of nutritional composition correction model for rape straw was verified by internal and external validation.
引文
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[4]常娟,卢敏,尹清强,郑秋红,郭红伟,樊春光.秸秆资源预处理研究进展.中国农学通报,2012,28(11):1-8.CHANG J,LU M,YIN Q Q,ZHENG Q H,GUO H W,FAN C G.Progress of research on pretreatment of corn stover.Chinese Agricultural Science Bulletin,2012,28(11):1-8.
[5]乌兰,马伟杰,义如格勒图,崔仲楠,鲁兴华.油菜秸秆饲用价值分析及其开发利用.内蒙古草业,2007,19(1):41-42.WU L,MA W J,Yiru Geletu,CUI Z N,LU X H.Analysis on feeding value of rape straw and its development and utilization.Inner Mongolia Prataculture,2007,19(1):41-42.
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[7]张勇,郭海明,汤志宏,曹凯,王雁坡,叶均安.油菜秆颗粒料对湖羊生产性能、瘤胃发酵参数及血液生化指标的影响.草业学报,2016,25(10):171-179.ZHANG Y,GUO H M,TANG Z H,CAO K,WANG Y P,YE J A.Effect of pelleted rape straw feed on productive performance,rumen fermentation parameters,and blood biochemical indexes of Hu sheep.Acta Prataculturae Sinica,2016,25(10):171-179.
[8]张吉鹍,李龙瑞.花生藤、红薯藤与油菜秸秆饲用品质的评定.江西农业大学学报,2016,38(4):754-759.ZHANG J K,LI L R.A comprehensive evaluation of feeding quality of peanut vine,sweet potato vine and rape straw.Acta Agriculturae Universitatis Jiangxiensis,2016,38(4):754-759.
[9]RAHMAN A,BAYRAM I,KHANUM S,ULLAH S.Use and calibration of near infrared reflectance spectroscopy in feed analysis:A mini review.Pakistan Journal of Life and Social Sciences,2015,13(1):1-7.
[10]张瑞忠,董宽虎.近红外技术在牧草方面的应用.草业科学,2007,24(6):14-19.ZHANG R Z,DONG K H.Application of near infrared reflectance dpectroscopy(NIRS)to forage aspect.Pratacultural Science,2007,24(6):14-19.
[11]毕于运.秸秆资源评价与利用研究.北京:中国农业科学院博士学位论文,2010.BI Y Y.Study on straw resources evaluation and utilization in China.PhD Thesis.Beijing:Chinese Academy of Agricultural Sciences,2010.
[12]NORRIS K H,BARNES R F,MOORE J E,SHENK J S.Predicting forage quality by infrared reflectance spectroscopy.Journal of Animal Science,1976,43(4):889-897.
[13]SOTOBARAJAS M C,ZABALGOGEAZCOA I,GONZáLEZMARTIN I,VáZQUEZDEALDANA B R.Qualitative and quantitative analysis of endophyte alkaloids in perennial ryegrass using near-infrared spectroscopy.Journal of the Science of Food and Agriculture,2017,97(14):5028-5036.
[14]PRIETO N,ROEHE R,LAVíN P,BATTEN G,ANDRéS S.Application of near infrared reflectance spectroscopy to predict meat and meat products quality:A review.Meat Science,2009,83(2):175-186.
[15]MELFSEN A,HOLSTERMANN M,HAEUSSERMANN A,MOLKENTIN J,SUSENBETH A,HARTUNG E.Accuracy and application of milk fatty acid estimation with diffuse reflectance near-infrared spectroscopy.Journal of Dairy Research,2018,85(2):212-221.
[16]李玉鹏.近红外反射光谱快速评定棉粕营养价值的研究.兰州:甘肃农业大学硕士学位论文,2016.LI Y P.Determination of nutrient values on cottonseed meal by near-infrared reflectance spectroscopy.Master Thesis.Lanzhou:Gansu Agriculture University,2016.
[17]李军涛.近红外反射光谱快速评定玉米和小麦营养价值的研究.北京:中国农业大学博士学位论文,2014.LI J T.Study on the rapid evaluation of nutrient values of corn and wheat by near-infrared reflectance spectroscopy.PhD Thesis.Beijing:China Agriculture University,2014.
[18]史永刚,冯新泸,李子存,孙萍.近红外光谱分析中应考虑的几个问题.光谱实验室,2001,18(4):435-437.SHI Y G,FENG X L,LI Z C,SUN P.Consideration of some problems in near-infrared spectra analysia.Chinese Journal of Spectroscopy Laboratory,2001,18(4):435-437.
[19]YANG Z,Nie G,PAN L,ZHANG Y,HUANG L,Ma X,ZHANG X Q.Development and validation of near-infrared spectroscopy for the prediction of forage quality parameters in Lolium multiflorum.Peerj,2017,5(10):3867-3886.
[20]ASEKOVA S.Determination of forage quality by near-infrared reflectance spectroscopy in soybean.Journal of Near Infrared Spectroscopy,2016,16(2):105-110.
[21]刘贤,韩鲁佳.近红外漫反射光谱法快速测定秸秆青贮饲料成分含量.光谱学与光谱分析,2006,26(11):2016-2020.LIU X,HAN L J.Feasibility of using near-infrared reflectance spectroscopy for the analysis of compositions of straw silage.Spectroscopy and Spectral Analysis,2006,26(11):2016-2020.
[22]白琪林,陈绍江,董晓玲,孟庆祥,严衍禄,戴景瑞.近红外漫反射光谱法测定玉米秸秆NDF与ADF含量.光谱学与光谱分析,2004,24(11):1345-1347.BAI Q L,CHEN S J,DONG X L,MENG Q X,YAN Y L,DAI J R.Prediction of NDF and ADF concentrations with near-infrared reflectance spectroscopy.Spectroscopy and Spectral Analysis,2004,24(11):1345-1347.
[23]邰书静,张仁和,史俊通,薛吉全,张兴华,马国胜,路海东.近红外光谱法测定玉米秸秆饲用品质.农业工程学报,2009,25(12):151-155.TAI S J,ZHANG R H,SHI J T,XUE J Q,ZHANG X H,MA G S,LU H D.Prediction of forage quality of maize stover by near infrared spectroscopy.Transactions of the CSAE,2009,25(12):151-155.
[24]杨天辉,常生华,莫本田,侯扶江.黄土高原13种栽培牧草营养成分NIRS模型分析.草业科学,2017,34(3):571-585.YANG T H,CHANG S H,MO B T,HOU F J.Analysis of nutrition content of in 13 forage crop varieties in the Loess Plateau based on visible/near infrared reflectance spectroscopy.Pratacultural Science,2017,34(3):571-585.
[2]SHEN D K,GU S,LUO K H,BRIDGWATER A V,FANG M X.Kinetic study on thermal decomposition of woods in oxidative environment.Fuel,2009,88(6):1024-1030.
[3]贾存辉,钱文熙,吐尔逊阿依·赛买提,敖维平,古力皮叶木·阿布都克然木.粗饲料营养价值指数及评定方法.草业科学,2017,34(2):415-427.JIA C H,QIAN W X,Tursunay·Samat,AO W P,Gulpiya·Abdukirem.Roughage nutritional value evaluation indices and research methods.Pratacultural Science,2017,34(2):415-427.
[4]常娟,卢敏,尹清强,郑秋红,郭红伟,樊春光.秸秆资源预处理研究进展.中国农学通报,2012,28(11):1-8.CHANG J,LU M,YIN Q Q,ZHENG Q H,GUO H W,FAN C G.Progress of research on pretreatment of corn stover.Chinese Agricultural Science Bulletin,2012,28(11):1-8.
[5]乌兰,马伟杰,义如格勒图,崔仲楠,鲁兴华.油菜秸秆饲用价值分析及其开发利用.内蒙古草业,2007,19(1):41-42.WU L,MA W J,Yiru Geletu,CUI Z N,LU X H.Analysis on feeding value of rape straw and its development and utilization.Inner Mongolia Prataculture,2007,19(1):41-42.
[6]刘建胜.我国秸秆资源分布及利用现状的分析.北京:中国农业大学硕士学位论文,2005.LIU J S.Analyses on the distribution pattern of the crop-straw resource and the status quo of its application in China.Master Thesis.Beijing:China Agriculture University,2005.
[7]张勇,郭海明,汤志宏,曹凯,王雁坡,叶均安.油菜秆颗粒料对湖羊生产性能、瘤胃发酵参数及血液生化指标的影响.草业学报,2016,25(10):171-179.ZHANG Y,GUO H M,TANG Z H,CAO K,WANG Y P,YE J A.Effect of pelleted rape straw feed on productive performance,rumen fermentation parameters,and blood biochemical indexes of Hu sheep.Acta Prataculturae Sinica,2016,25(10):171-179.
[8]张吉鹍,李龙瑞.花生藤、红薯藤与油菜秸秆饲用品质的评定.江西农业大学学报,2016,38(4):754-759.ZHANG J K,LI L R.A comprehensive evaluation of feeding quality of peanut vine,sweet potato vine and rape straw.Acta Agriculturae Universitatis Jiangxiensis,2016,38(4):754-759.
[9]RAHMAN A,BAYRAM I,KHANUM S,ULLAH S.Use and calibration of near infrared reflectance spectroscopy in feed analysis:A mini review.Pakistan Journal of Life and Social Sciences,2015,13(1):1-7.
[10]张瑞忠,董宽虎.近红外技术在牧草方面的应用.草业科学,2007,24(6):14-19.ZHANG R Z,DONG K H.Application of near infrared reflectance dpectroscopy(NIRS)to forage aspect.Pratacultural Science,2007,24(6):14-19.
[11]毕于运.秸秆资源评价与利用研究.北京:中国农业科学院博士学位论文,2010.BI Y Y.Study on straw resources evaluation and utilization in China.PhD Thesis.Beijing:Chinese Academy of Agricultural Sciences,2010.
[12]NORRIS K H,BARNES R F,MOORE J E,SHENK J S.Predicting forage quality by infrared reflectance spectroscopy.Journal of Animal Science,1976,43(4):889-897.
[13]SOTOBARAJAS M C,ZABALGOGEAZCOA I,GONZáLEZMARTIN I,VáZQUEZDEALDANA B R.Qualitative and quantitative analysis of endophyte alkaloids in perennial ryegrass using near-infrared spectroscopy.Journal of the Science of Food and Agriculture,2017,97(14):5028-5036.
[14]PRIETO N,ROEHE R,LAVíN P,BATTEN G,ANDRéS S.Application of near infrared reflectance spectroscopy to predict meat and meat products quality:A review.Meat Science,2009,83(2):175-186.
[15]MELFSEN A,HOLSTERMANN M,HAEUSSERMANN A,MOLKENTIN J,SUSENBETH A,HARTUNG E.Accuracy and application of milk fatty acid estimation with diffuse reflectance near-infrared spectroscopy.Journal of Dairy Research,2018,85(2):212-221.
[16]李玉鹏.近红外反射光谱快速评定棉粕营养价值的研究.兰州:甘肃农业大学硕士学位论文,2016.LI Y P.Determination of nutrient values on cottonseed meal by near-infrared reflectance spectroscopy.Master Thesis.Lanzhou:Gansu Agriculture University,2016.
[17]李军涛.近红外反射光谱快速评定玉米和小麦营养价值的研究.北京:中国农业大学博士学位论文,2014.LI J T.Study on the rapid evaluation of nutrient values of corn and wheat by near-infrared reflectance spectroscopy.PhD Thesis.Beijing:China Agriculture University,2014.
[18]史永刚,冯新泸,李子存,孙萍.近红外光谱分析中应考虑的几个问题.光谱实验室,2001,18(4):435-437.SHI Y G,FENG X L,LI Z C,SUN P.Consideration of some problems in near-infrared spectra analysia.Chinese Journal of Spectroscopy Laboratory,2001,18(4):435-437.
[19]YANG Z,Nie G,PAN L,ZHANG Y,HUANG L,Ma X,ZHANG X Q.Development and validation of near-infrared spectroscopy for the prediction of forage quality parameters in Lolium multiflorum.Peerj,2017,5(10):3867-3886.
[20]ASEKOVA S.Determination of forage quality by near-infrared reflectance spectroscopy in soybean.Journal of Near Infrared Spectroscopy,2016,16(2):105-110.
[21]刘贤,韩鲁佳.近红外漫反射光谱法快速测定秸秆青贮饲料成分含量.光谱学与光谱分析,2006,26(11):2016-2020.LIU X,HAN L J.Feasibility of using near-infrared reflectance spectroscopy for the analysis of compositions of straw silage.Spectroscopy and Spectral Analysis,2006,26(11):2016-2020.
[22]白琪林,陈绍江,董晓玲,孟庆祥,严衍禄,戴景瑞.近红外漫反射光谱法测定玉米秸秆NDF与ADF含量.光谱学与光谱分析,2004,24(11):1345-1347.BAI Q L,CHEN S J,DONG X L,MENG Q X,YAN Y L,DAI J R.Prediction of NDF and ADF concentrations with near-infrared reflectance spectroscopy.Spectroscopy and Spectral Analysis,2004,24(11):1345-1347.
[23]邰书静,张仁和,史俊通,薛吉全,张兴华,马国胜,路海东.近红外光谱法测定玉米秸秆饲用品质.农业工程学报,2009,25(12):151-155.TAI S J,ZHANG R H,SHI J T,XUE J Q,ZHANG X H,MA G S,LU H D.Prediction of forage quality of maize stover by near infrared spectroscopy.Transactions of the CSAE,2009,25(12):151-155.
[24]杨天辉,常生华,莫本田,侯扶江.黄土高原13种栽培牧草营养成分NIRS模型分析.草业科学,2017,34(3):571-585.YANG T H,CHANG S H,MO B T,HOU F J.Analysis of nutrition content of in 13 forage crop varieties in the Loess Plateau based on visible/near infrared reflectance spectroscopy.Pratacultural Science,2017,34(3):571-585.