多倍体萱草的离体快繁技术
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摘要
以多倍体萱草的嫩叶、生长点、腋芽、花蕾、花瓣和花茎作外植体进行离体快繁技术研究。结果表明:以幼嫩的花蕾和花瓣为外植体最为适宜;以MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.7 mg/L为最适诱导培养基,诱导分化率可达74%以上;以MS+6-BA 2.0 mg/L+NAA 0.2 mg/L为最适继代增殖培养基,增殖系数可达5.8;以MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+IBA 0.02 mg/L+马铃薯20 g/L为壮苗培养基。培养1~2周后转入MS+NAA0.04 mg/L的生根培养基中,其平均根长、根长势等最为理想,生根率达100%,且移栽后成活率高,生长健壮。
The experiment was conducted to study the rapid in vitro propagation of polyploidy Hemerocallis fulva with tender leaf,growing point,axillary bud,bud,petal and scape as explants.The young bud and petal were the most suitable explants.MS+6-BA 0.5 mg / L+NAA 0.2 mg / L+2,4-D 0.7 mg / L was the most suitable inducing medium,and the inducing differentiation rate was 74%.MS+6-BA 2.0 mg / L+NAA 0.2 mg / L was the most suitable subculture medium,and proliferation coefficient was 5.8.MS+6-BA 1.0 mg / L+NAA 0.2 mg / L+IBA 0.02 mg / L+Potato 20 g / L was the strong seedling medium.After 1-2 weeks,tissue culture seedling was transferred to the rooting medium of MS+NAA 0.04 mg / L.The average root length and root growth of tissue culture seedlings were the most ideal with the rooting rate of 100%,the survival rate was high,and the growth was strong.
The experiment was conducted to study the rapid in vitro propagation of polyploidy Hemerocallis fulva with tender leaf,growing point,axillary bud,bud,petal and scape as explants.The young bud and petal were the most suitable explants.MS+6-BA 0.5 mg / L+NAA 0.2 mg / L+2,4-D 0.7 mg / L was the most suitable inducing medium,and the inducing differentiation rate was 74%.MS+6-BA 2.0 mg / L+NAA 0.2 mg / L was the most suitable subculture medium,and proliferation coefficient was 5.8.MS+6-BA 1.0 mg / L+NAA 0.2 mg / L+IBA 0.02 mg / L+Potato 20 g / L was the strong seedling medium.After 1-2 weeks,tissue culture seedling was transferred to the rooting medium of MS+NAA 0.04 mg / L.The average root length and root growth of tissue culture seedlings were the most ideal with the rooting rate of 100%,the survival rate was high,and the growth was strong.
引文
[1]张洁茹,刘晓嘉,陈丽飞,等.矮壮素对萱草组培苗生根及移栽的影响[J].东北林业大学学报,2014,42(7):95-99.
[2]张洁茹,刘晓嘉,陈丽飞,等.萱草优良单株花茎离体快繁[J].东北林业大学学报,2014,42(2):73-77.
[3]毕晓颖,王宁.萱草花茎离体培养与快速繁殖[J].东北林业大学学报,2012,40(11):56-59,158.
[4]兰丽婷,李冲,任爽英,等.萱草新品种组培再生体系的建立[J].东北林业大学学报,2011,39(4):14-17.
[5]姜凤英,栾绍武,吴志刚,等.多倍体萱草“金娃娃”的离体培养研究[J].辽宁农业科学,2007(1):51-52.
[6]路光,王岩,涂传炜.大花萱草—金娃娃组培苗继代增殖因素的研究[J].北方园艺,2006(6):139-140.
[7]王晓娟,金樑,陈家宽.大花萱草不同外植体诱导愈伤组织的比较研究[J].生命科学研究,2005,9(3):242-246.
[2]张洁茹,刘晓嘉,陈丽飞,等.萱草优良单株花茎离体快繁[J].东北林业大学学报,2014,42(2):73-77.
[3]毕晓颖,王宁.萱草花茎离体培养与快速繁殖[J].东北林业大学学报,2012,40(11):56-59,158.
[4]兰丽婷,李冲,任爽英,等.萱草新品种组培再生体系的建立[J].东北林业大学学报,2011,39(4):14-17.
[5]姜凤英,栾绍武,吴志刚,等.多倍体萱草“金娃娃”的离体培养研究[J].辽宁农业科学,2007(1):51-52.
[6]路光,王岩,涂传炜.大花萱草—金娃娃组培苗继代增殖因素的研究[J].北方园艺,2006(6):139-140.
[7]王晓娟,金樑,陈家宽.大花萱草不同外植体诱导愈伤组织的比较研究[J].生命科学研究,2005,9(3):242-246.