优质耐阴观叶植物熊掌木的离体快繁研究
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摘要
以熊掌木当年生嫩茎为外植体,以MS培养基为基础培养基,附加不同种类和浓度的植物生长调节物质诱导丛生芽及再生植株,建立熊掌木的高效再生繁殖体系。结果表明:先用75%酒精浸泡30 s,再用0.1%HgCl_2浸泡6 min的灭菌效果最佳;最佳增殖培养基为MS+6-BA 4 mg·L~(-1)+NAA 0.2 mg·L~(-1);在培养基MS+6-BA 4 mg·L~(-1)+NAA 0.2 mg·L~(-1)+蔗糖3.0%+琼脂0.7%+AgNO_30.1 mg·L~(-1)上,试管苗玻璃化程度最低,且芽增殖倍数最高;最佳生根培养基为1/2MS+NAA 0.1 mg·L~(-1)+IBA 0.01 mg·L~(-1),试管苗根系质量最好;将试管苗移栽在河沙︰珍珠岩︰草炭土(1︰1︰1)的基质上,成活率高达94%。
In this study,to establish the high frequency propagation system of Fatshedrea lizei,the annual tender steem of Fatshedrea lizei was taken as explant and MS medium as basic medium,multiple shoots and regeneration plants were induced by adding differernt kinds and concentrations of plant growth regulators.Rusult indicated:the best sterilization procedure for shoot tip was soaked in 75%alcohol for 30s and followed by 0.1%HgCl_26 minutes.The optimum medium for bud proliferation was MS medium with 6-BA4 mg·L~(-1)+NAA0.2 mg·L~(-1),which could produce a large number of adventitious buds.In the MS medium with 6-BA4 mg·L~(-1)+NAA0.2 mg·L~(-1)+Sucrose 3.0%+Agar 0.7%+AgNO_30.1 mg·L~(-1),the vitrification degree of Fatshedrea lizei was the lowest and the bud proliferation was the highest.The optimum rooting medium was half MS medium with NAA 0.1 mg·L~(-1)+IBA 0.01 mg·L~(-1).The transplant survival rate was high on the transplant medium composed of sand:pearlite:peat=1:1:1,survival rate was up to 94%.
In this study,to establish the high frequency propagation system of Fatshedrea lizei,the annual tender steem of Fatshedrea lizei was taken as explant and MS medium as basic medium,multiple shoots and regeneration plants were induced by adding differernt kinds and concentrations of plant growth regulators.Rusult indicated:the best sterilization procedure for shoot tip was soaked in 75%alcohol for 30s and followed by 0.1%HgCl_26 minutes.The optimum medium for bud proliferation was MS medium with 6-BA4 mg·L~(-1)+NAA0.2 mg·L~(-1),which could produce a large number of adventitious buds.In the MS medium with 6-BA4 mg·L~(-1)+NAA0.2 mg·L~(-1)+Sucrose 3.0%+Agar 0.7%+AgNO_30.1 mg·L~(-1),the vitrification degree of Fatshedrea lizei was the lowest and the bud proliferation was the highest.The optimum rooting medium was half MS medium with NAA 0.1 mg·L~(-1)+IBA 0.01 mg·L~(-1).The transplant survival rate was high on the transplant medium composed of sand:pearlite:peat=1:1:1,survival rate was up to 94%.
引文
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[16]霍朗宁,张东升,贾忠奎.树莓‘沙尼’组培技术[J].东北林业大学学报,2015,43(3):6-9.
[2]王陆军,沈后海,蔡新玲.熊掌木等3种植物耐荫性的比较研究[J].安徽林业科技,2011,37(2):16-19.
[3]陈丽静,刘少霞,马慧,等.刚健芦荟的组织培养和快速繁殖[J].植物生理学通讯,2000,36(4):339.
[4]陈丽静,潘英,马慧,等.食用仙人掌离体培养和快速繁殖[J].园艺学报,2001(4):327-331.
[5]沈海龙.植物组织培养[M].北京:中国林业出版社,2005.
[6]李永欣,王晓明,陈明皋,等.外源激素对美洲冬青组织培养的影响[J].中南林业科技大学学报,2010,30(1):65-68.
[7]张俊林,余有祥,沈柏春,等.北美冬青‘奥斯特’的组织培养和快速繁殖[J].植物生理学报,2014,50(10):1541-1545.
[8]邹义萍.北美冬青快繁技术研究[D].长沙:中南林业科技大学,2014.
[9]HIDEHIRO F,YOKO O,MASSO T.Regulation of lateral root formation by auxin signaling in Arabidopsis[J].Plant Biotechnology,2005,22(5):393-399.
[10]尹立辉,武术杰,刘亚亮,等.多倍体萱草的离体快繁技术[J].东北林业大学学报,2016,44(8):41-43.
[11]吴雯雯,陆兵,李文清,等.留兰香薄荷离体快繁技术研究[J].江苏农业科技,2018,46(4):47-49.
[12]安佰义,郭才南,包文慧,等.白檀离体快繁技术[J].植物学报,2018,53(5):693-699.
[13]孙姿.夏蜡梅组织培养技术研究[D].南京:南京林业大学,2015.
[14]Yang Y,Jiang L J,Tang Y X,et al.Organogenesis and regeneration of Symplocos paniculata from immature zygotic embryos in vitro[J].Northern Hortic,2016,40(5):119-123.
[15]施琼,胡峰,黄烈健,等.马大杂种相思腋芽高效组培增殖体系[J].林业科学,2014,50(6):55-60.
[16]霍朗宁,张东升,贾忠奎.树莓‘沙尼’组培技术[J].东北林业大学学报,2015,43(3):6-9.