保存温度及时间对化学发光法测定直接肾素浓度的影响
|
摘要
目的研究样本保存温度及时间对血浆直接肾素浓度(DRC)的影响。方法采集乙二胺四乙酸二钾(EDTA-K2)抗凝血并分离血浆,于室温和冷藏均分别放置2、4、6、8h;冷冻4、8、12周;冷冻冻融1～3次后采用化学发光法测定DRC,与即刻值比较,差异率超过总变化极限值认为不可接受。结果室温放置2、4、6、8h的DRC均低于即刻值(P<0.05),差异率超过17%的样本比例分别为0、0、1/20、4/20;冷藏2、4、6、8h的DRC均高于即刻值(P<0.05),差异率超过17%的样本比例分别为0、2/20、4/20、11/20;冷冻12周内所有样本差异率均<17%;冻融2次时有1个样本降低超过17%。结论温度影响DRC稳定性,建议样本采集后室温6h内检测,否则应分离血浆冻存于-20℃可稳定12周,避免反复冻融。
Objective To study the effects of sample storage temperature and time on plasma direct renin concentration(DRC).Methods Potassium ethylenediaminetetraacetate(EDTA-K2)anticoagulant boold was collected and plasma was separated.The plasma was stored at room temperature and refrigerated for 2,4,6 and 8 h,and frozen for 4,8 and 12 weeks.After freezing and thawing for 1 to 3 times,the chemiluminescence method was used to determine DRC.Compared with the instant value,the difference rate exceeding the total change limit was considered unacceptable.Results DRC values of 2,4,6 and 8 hat room temperature were lower than immediate values(P<0.05).The proportion of samples with difference rate over 17% was 0,0,1/20,4/20,respectively.DRC values of 2,4,6 and 8 hof refrigeration were higher than immediate value(P<0.05).The difference rate of all samples within 12 weeks of freezing was less than 17%.One sample decreased by more than 17%after freezing and thawing twice.Conclusion Temperature affects the stability of DRC.It is suggested that the samples should be detected within 6 hours after collection.Otherwise,the plasma should be frozen and stored at-20℃for 12 weeks to avoid repeated freezing and thawing.
Objective To study the effects of sample storage temperature and time on plasma direct renin concentration(DRC).Methods Potassium ethylenediaminetetraacetate(EDTA-K2)anticoagulant boold was collected and plasma was separated.The plasma was stored at room temperature and refrigerated for 2,4,6 and 8 h,and frozen for 4,8 and 12 weeks.After freezing and thawing for 1 to 3 times,the chemiluminescence method was used to determine DRC.Compared with the instant value,the difference rate exceeding the total change limit was considered unacceptable.Results DRC values of 2,4,6 and 8 hat room temperature were lower than immediate values(P<0.05).The proportion of samples with difference rate over 17% was 0,0,1/20,4/20,respectively.DRC values of 2,4,6 and 8 hof refrigeration were higher than immediate value(P<0.05).The difference rate of all samples within 12 weeks of freezing was less than 17%.One sample decreased by more than 17%after freezing and thawing twice.Conclusion Temperature affects the stability of DRC.It is suggested that the samples should be detected within 6 hours after collection.Otherwise,the plasma should be frozen and stored at-20℃for 12 weeks to avoid repeated freezing and thawing.
引文
[1] FUNDER J W,CAREY R M,MANTERO F,et al.The management of primary aldosteronism:case detection,diagnosis,and treatment:an endocrine society clinical practice guideline[J].J Clin Endocrinol Metab,2016,101(5):1889-1916.
[2] SANG X J,JIANG Y R,WANG W Q,et al.Prevalence of and risk factors for primary aldosteronism among patients with resistant hypertension in China[J].J Hypertens,2013,31(7):1465-1471.
[3] MULATERO P,STOWASSER M,LOH K C,et al.Increased diagnosis of primary aldosteronism,including surgically correctable forms,in centers from five continents[J].J Clin Endocrinol Metab,2004,89(3):1045-1050.
[4]中华医学会内分泌学分会肾上腺学组.原发性醛固酮增多症诊断治疗的专家共识[J].中华内分泌代谢杂志,2016,32(3):188-195.
[5] BURRELLO J,MONTICONE S,BUFFOLO F,et al.Diagnostic accuracy of aldosterone and renin measurement by chemiluminescent immunoassay and radioimmunoassay in primary aldosteronism[J].J Hypertens,2016,34(5):920-927.
[6] LONATI C,BASSANI N,GRITTI A,et al.Measurement of plasma renin concentration instead of plasma renin activity decreases the positive aldosterone-to-renin ratio tests in treated patients with essential hypertension[J].J Hypertens,2014,32(3):627-634.
[7]姚一帆.温度对血管紧张素Ⅱ及肾素活性结果影响的研究[J].实验与检验医学,2013,6:549-551.
[8] GRUSON D,MAISIN D,LISON P,et al.Two-site automated chemiluminescent assay for measurement of immunoreactive renin[J].Biomarkers,2011,16(7):605-609.
[9] GLINICKI P,JESKE W,GIETKA-CZERNEL M,et al.The effect of blood collection procedure on plasma renin activity(PRA)and concentrations of direct renin(DRC)and aldosterone[J].J Renin Angiotensin Aldosterone Syst,2015,16(2):339-343.
[10]LOCSEI Z,RACZ K,PATOCS A,et al.Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration[J].Clin Chim Acta,2009,402(1/2):203-205.
[11]李溪月,RICHA G,胡金波等.不同贮存条件对肾素和醛固酮浓度测定的影响[J].重庆医科大学学报,2016,11(11):1101-1104.
[12]BUORO S,MECCA T,SEGHEZZI M,et al.Assessment of blood sample stability for complete blood count using the Sysmex XN-9000and Mindray BC-6800analyzers[J].Rev Bras Hematol Hemoter,2016,38(3):225-239.
[13]ODDOZE C,LOMBARD E,PORTUGAL H.Stability study of 81analytes in human whole blood,in serum and in plasma[J].Clin Biochem,2012,45(6):464-469.
[14] MEIJERS W C,VAN DER VELDE A R,MULLER KOBOLD A C,et al.Variability of biomarkers in patients with chronic heart failure and healthy controls[J].Eur J Heart Fail,2017,19(3):357-365.
[15]CAMPBELL D J,NUSSBERGER J,STOWASSER M,et al.Activity assays and immunoassays for plasma Renin and prorenin:information provided and precautions necessary for accurate measurement[J].Clin Chem,2009,55(5):867-877.
[16]DENG L,XIONG Z,LI H,et al.Analytical validation and investigation on reference intervals of aldosterone and renin in Chinese Han population by using fully automated chemiluminescence immunoassays[J].Clin Biochem.2018;56:89-94.
[2] SANG X J,JIANG Y R,WANG W Q,et al.Prevalence of and risk factors for primary aldosteronism among patients with resistant hypertension in China[J].J Hypertens,2013,31(7):1465-1471.
[3] MULATERO P,STOWASSER M,LOH K C,et al.Increased diagnosis of primary aldosteronism,including surgically correctable forms,in centers from five continents[J].J Clin Endocrinol Metab,2004,89(3):1045-1050.
[4]中华医学会内分泌学分会肾上腺学组.原发性醛固酮增多症诊断治疗的专家共识[J].中华内分泌代谢杂志,2016,32(3):188-195.
[5] BURRELLO J,MONTICONE S,BUFFOLO F,et al.Diagnostic accuracy of aldosterone and renin measurement by chemiluminescent immunoassay and radioimmunoassay in primary aldosteronism[J].J Hypertens,2016,34(5):920-927.
[6] LONATI C,BASSANI N,GRITTI A,et al.Measurement of plasma renin concentration instead of plasma renin activity decreases the positive aldosterone-to-renin ratio tests in treated patients with essential hypertension[J].J Hypertens,2014,32(3):627-634.
[7]姚一帆.温度对血管紧张素Ⅱ及肾素活性结果影响的研究[J].实验与检验医学,2013,6:549-551.
[8] GRUSON D,MAISIN D,LISON P,et al.Two-site automated chemiluminescent assay for measurement of immunoreactive renin[J].Biomarkers,2011,16(7):605-609.
[9] GLINICKI P,JESKE W,GIETKA-CZERNEL M,et al.The effect of blood collection procedure on plasma renin activity(PRA)and concentrations of direct renin(DRC)and aldosterone[J].J Renin Angiotensin Aldosterone Syst,2015,16(2):339-343.
[10]LOCSEI Z,RACZ K,PATOCS A,et al.Influence of sampling and storage conditions on plasma renin activity and plasma renin concentration[J].Clin Chim Acta,2009,402(1/2):203-205.
[11]李溪月,RICHA G,胡金波等.不同贮存条件对肾素和醛固酮浓度测定的影响[J].重庆医科大学学报,2016,11(11):1101-1104.
[12]BUORO S,MECCA T,SEGHEZZI M,et al.Assessment of blood sample stability for complete blood count using the Sysmex XN-9000and Mindray BC-6800analyzers[J].Rev Bras Hematol Hemoter,2016,38(3):225-239.
[13]ODDOZE C,LOMBARD E,PORTUGAL H.Stability study of 81analytes in human whole blood,in serum and in plasma[J].Clin Biochem,2012,45(6):464-469.
[14] MEIJERS W C,VAN DER VELDE A R,MULLER KOBOLD A C,et al.Variability of biomarkers in patients with chronic heart failure and healthy controls[J].Eur J Heart Fail,2017,19(3):357-365.
[15]CAMPBELL D J,NUSSBERGER J,STOWASSER M,et al.Activity assays and immunoassays for plasma Renin and prorenin:information provided and precautions necessary for accurate measurement[J].Clin Chem,2009,55(5):867-877.
[16]DENG L,XIONG Z,LI H,et al.Analytical validation and investigation on reference intervals of aldosterone and renin in Chinese Han population by using fully automated chemiluminescence immunoassays[J].Clin Biochem.2018;56:89-94.