共价有机笼CC3-R毛细管电色谱柱用于拆分扁桃酸化合物的研究
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摘要
该文合成了一种具有三维钻石网络状通道的单一手性POC材料(CC3-R),将其用作手性固定相制备CC3-R毛细管电色谱柱,考察了其手性识别性能,并测试了电流随电压的变化及电渗流随缓冲溶液pH值的变化,然后以该柱对扁桃酸进行了拆分。结果显示,D-扁桃酸和L-扁桃酸得到了基线分离,分离度为1.81,理论塔板数分别为36 700 N/m和41 600 N/m,同一根CC3-R毛细管电色谱柱对扁桃酸的保留时间的相对标准偏差为1.2%。表明CC3-R手性柱不仅有良好的手性识别能力还具有良好的稳定性和重现性。
A capillary electrochromatography with covalent porous organic cage chiral material(CC3-R) was developed for the separation of mandelic acid.The single CC3-R with three-dimensional diamond network channel was synthesized,and used as a chiral stationary phase to prepare a capillary electrochromatography column.The chiral recognition performance for CC3-R capillary electrochromatography column was investigated.The variations of current with voltage and electroosmotic flow with pH value of buffer solution were measured,and then this column was used to separate mandelic acid.Results showed that the baseline separations for D-mandelic acid and L-mandelic acid were obtained with the degree of separation of 1.81,and the numbers of theoretical tray for them both were 36 700 N/m and 41 600 N/m,respectively.The retention time of mandelic acid on the same CC3-R capillary column was observed with the relative standard deviation of 1.2%.Results showed that the CC3-R chiral column not only has a good chiral recognition ability,but also has good stability and reproducibility.
A capillary electrochromatography with covalent porous organic cage chiral material(CC3-R) was developed for the separation of mandelic acid.The single CC3-R with three-dimensional diamond network channel was synthesized,and used as a chiral stationary phase to prepare a capillary electrochromatography column.The chiral recognition performance for CC3-R capillary electrochromatography column was investigated.The variations of current with voltage and electroosmotic flow with pH value of buffer solution were measured,and then this column was used to separate mandelic acid.Results showed that the baseline separations for D-mandelic acid and L-mandelic acid were obtained with the degree of separation of 1.81,and the numbers of theoretical tray for them both were 36 700 N/m and 41 600 N/m,respectively.The retention time of mandelic acid on the same CC3-R capillary column was observed with the relative standard deviation of 1.2%.Results showed that the CC3-R chiral column not only has a good chiral recognition ability,but also has good stability and reproducibility.
引文
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[3] Liu Z H,Ji Y S,Zhang J,Huang H N,Liang B.J.Instrum.Anal.(刘志华,纪永升,张静,黄华宁,粱蓓.分析测试学报),2015,34(8):939-943.
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[6] Zhang J H,Xie S M,Chen L,Wang B J,He P G,Yuan L M.Anal.Chem.,2015,87(15):7817-7824.
[7] Xie S M,Zhang J H,Fu N,Wang B J,Chen L,Yuan L M.Anal.Chim.Acta,2016,903:156-163.
[8] Zhang J H,Xie S M,Wang B J,He P G,Yuan L M.J.Chromatogr.A,2015,1426:174-182.
[9] Hasell T,Chong S Y,Schmidtmann M,Adams D J,Cooper A I.Angew.Chem.Int.Ed.,2012,51(29):7154-7157.
[10] Evans J D,Huang D M,Hill M R,Sumby C J,Thornton A W,Doonan C J.J.Phys.Chem.C,2014,118(3):1523-1529.
[11] Li W,Zhang J.J.Comput.Chem.,2013,35(2):174-180.
[12] Zhang J H,Zhu P J,Xie S M,Zi M,Yuan L M.Anal.Chim.Acta,2017,999:169-175.
[13] Tozawa T,Jones J T A,Swamy S I,Jiang S,Adams D J,Shakespeare S,Clowes R,Bradshaw D,Hasell T,Chong S Y,Tang C,Thompson S,Parker J,Trewin A,Bacsa J,Slawin A M Z,Steiner A,Cooper A I.Nat.Mater.,2009,8(12):973-978.
[14] Cooper A I.Angew.Chem.Int.Ed.,2011,50(5):996-998.
[15] Zhu P J,Tao Y,Zhang J H,Zi M,Yuan L M.Chin.J.Chromatogr.(朱鹏静,陶勇,章俊辉,字敏,袁黎明.色谱),2016,34(12):1219-1227.
[16] Mo R Z,Xu S J,Jin S,Wang W,Ji Y B.J.Instrum.Anal.(莫荣珍,徐树娟,金灿,王伟,季一兵.分析测试学报),2017,36(9):1081-1086.
[17] Nie G Z,Li L S,Cheng B P,Zhou R D,Zhang H F.Chem.J.Chin.Univ.(聂桂珍,李来生,程彪平,周仁丹,张宏福.高等学校化学学报),2014,35(7):1414-1422.
[18] Fei Z X,Zhang M,Zhang J H,Yuan L M.Anal.Chim.Acta,2014,830(22):49-55.