明胶添加量对α-磷酸三钙骨水泥性能的影响
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摘要
目的探讨明胶溶液添加量对α-磷酸三钙(α-tricalcium phosphate,α-TCP)骨水泥体系性能的影响。方法将不同比例的明胶溶液与α-TCP骨水泥体系调和,测量各组骨水泥试样的抗弯强度和抗压强度,与未添加明胶溶液的对照组进行比较。将力学强度提高最大的一组与对照组进行防水性能、凝固时间和电镜下表面形貌图对比观察。结果当明胶溶液与骨水泥调和液体积比(V明胶溶胶液∶V骨水泥调和液)=25∶100时,骨水泥试样的抗压强度和抗弯强度最大。与对照组相比,抗压强度由(7.874 54±0.660 97)MPa提高到(9.936 52±0.433 17)MPa,差异有统计学意义(P<0.05);抗弯强度由(5.157 06±0.298 30)MPa提高到(7.959 71±0.281 63)MPa,差异有统计学意义(P<0.05)。初凝时间由3min延长到5min,而终凝时间仍为13min。同时骨水泥防水性能增强,形成的羟基磷灰石晶体更加密集、均匀。结论适宜的明胶溶液添加比例可以提高α-TCP骨水泥的力学性能、防水性能,并获得更适合临床应用的凝固时间。
Objective To determine the effects of gelatin on the performance of calcium phosphate cement(CPC).Methods α-tricalcium phosphate(α-TCP)bone cement was mixed with different concentrations of gelatin solutions.The CPC samples were soaked into simulated body fluid for one day before their compressive and bending strengths were measured. We also compared their waterproof performance,solidification time and surface topography.Results Gelatin solutions changed the performance of CPC.Optimal performance of CPC was achieved when the volume ratio of gelatin solution to CPC(Vgelatin solution∶VCPC liquid)was set at 25∶100,which increased compressive strength from(7.874 54±0.660 97)MPa to(9.936 52±0.433 17)MPa and bending strength from(5.157 06±0.298 30)MPa to(7.959 71±0.281 63)MPa.Gelatin solution also prolonged setting time of CPC,improved its waterproof performance,and promoted formulation of more dense and uniform hydroxyapatite crystals.Conclusion Gelatin can improve the compressive and bending strengths of CPC,and make CPC more suitable for clinic use through improvements in setting time and waterproof performance.
Objective To determine the effects of gelatin on the performance of calcium phosphate cement(CPC).Methods α-tricalcium phosphate(α-TCP)bone cement was mixed with different concentrations of gelatin solutions.The CPC samples were soaked into simulated body fluid for one day before their compressive and bending strengths were measured. We also compared their waterproof performance,solidification time and surface topography.Results Gelatin solutions changed the performance of CPC.Optimal performance of CPC was achieved when the volume ratio of gelatin solution to CPC(Vgelatin solution∶VCPC liquid)was set at 25∶100,which increased compressive strength from(7.874 54±0.660 97)MPa to(9.936 52±0.433 17)MPa and bending strength from(5.157 06±0.298 30)MPa to(7.959 71±0.281 63)MPa.Gelatin solution also prolonged setting time of CPC,improved its waterproof performance,and promoted formulation of more dense and uniform hydroxyapatite crystals.Conclusion Gelatin can improve the compressive and bending strengths of CPC,and make CPC more suitable for clinic use through improvements in setting time and waterproof performance.
引文
[1]VARALAKSHMI PR,KAVITHA M,GOVINDAN R,et al.Effect of statins withα-tricalcium phosphate on proliferation,differentiation,and mineralization of human dental pulp cells.J Endod,2013,39(6):806-812.
[2]MARUKAWA K,UEKI K,OKABE K,et al.Use of selfsettingα-tricalcium phosphate for maxillary sinus augmentation in rabbit.Clin Oral Implants Res,2011,22(6):606-612.
[3]周馨,郑昌琼,王方瑚,等.骨水泥及磷酸钙生物活性骨水泥.硅酸盐通报,1998,17(5):33-38.
[4]ROJBANI H,NYAN M,OHYA K,et al.Evaluation of the osteoconductivity ofα-tricalcium phosphate,β-tricalcium phosphate,and hydroxyapatite combined with or without simvastatin in rat calvarial defect.J Biomed Mater Res A,2011,98(4):488-498.
[5]VARALAKSHMI PR,KAVITHA M,GOVINDAN R,et al.Effect of statins withα-tricalcium phosphate on proliferation,differentiation,and mineralization of human dental pulp cells.J Endod,2013,39(6):806-812.
[6]BIGI A,PANZAVOLTA S,RUBINI K.Relationship between triple-helix content and mechanical properties of gelatin films.Biomaterials,2004,25(25):5675-5680.
[7]TOUNY AH,LAURENCIN C,NAIR L,et al.Formation of composites comprised of calcium deficient HAp and crosslinked gelatin.J Mater Sci:Mater Med,2008,19(10):3193-3201.
[8]KUIJPERS AJ,ENGBERS GH,KRIJGSVELD J,et al.Cross-linking and characteri sterisation of gelatin matrices for biomedical applications.Biomater Sci Polym Ed,2000,11(3):225-243.
[9]MAO JS,CUI YL,WANG XH,et al.A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis.Biomaterials,2004,25(18):3971-3981.
[10]BIGI A,BRACCI B,PANZAVOLTA S.Effect of added gelatin on the properties of calcium phosphate cement.Biomaterials,2004,25(14):2893-2899.
[11]潘朝晖,范清宇,蔡和平,等.明胶对自固化磷酸钙骨水泥性能的影响.医学研究生学报,2005,18(8):696-699.
[12]王彦伟,蔡舒,姚康德.明胶溶胀行为对自固化多孔骨水泥性能和结构的影响.生物骨科材料与临床研究,2003,1(1):41-42.
[13]卫冬娟,张翔,顾建文,等.α-磷酸三钙/HA晶须/羧甲基壳聚糖-明胶复合增强多孔骨水泥的制备研究.生物医学工程学杂志,2012,29(3):491-495.
[14]DOI Y,TAKEZAWA Y,SHIBATA S,et al.Self-setting apatite cement,in physicochemical properties.J Japan Soc Dent Matals Devices,1987(6):53-58.
[15]QU Y,YANG Y,LI J,et al.Preliminary evaluation of a novel strong/osteoinductive calcium phosphate cement.J Biomater Appl,2011,26(3):311.
[16]YUAN H,LI Y,DE BRUIJN JD,et al.Tissue responses of calcium phospliate cement:a study in dogs.Biomaterials,2000,21(12):1283-1290.
[17]PANZAVOLTA S,TORRICELLI P,BRACCI B,et al.Functionalization of biomimetic calcium phosphate bone cements with alendronate.Inorg Biochem,2010,104(10):1099-1106.
[18]戴红莲,闫玉华,李世普,等.α-磷酸三钙-磷酸四钙生物骨水泥的研究.材料科学与工程,2002,20(3):331-335.
[19]LEROUX L,HATIM Z,FRECHE M,et al.Effect of various adjuvants(lactic acid,glycerol and chitosan)on the injectability of a calcium phosphate cement.Bone,1999,25(2Suppl):31S-34S.
[20]MISTRY AS,MIKOS AG.Tissue engineering strategies for bone regeneration.Adv Biochem Eng Biotechnol,2005(94):1-22.
[21]HUTMACHER DW.Scaffolds in tissue engineering bone and cartilage.Biomaterials,2000,21(24):2529-2543.
[22]DURUCAN C,BROWN PW.alpha-tricalcium phosphate hydrolysis to hydroxyapatite at and near physiological temperature.J Mater Sci Mater Med,2000,11(6):365-371.
[23]MONMA H,KANAZAWA T.The hydration ofα-tricalcium phosphate.J Cera Soc Jap,1976,84(4):209-213.
[24]JANSEN J,OOMS E,VERDONSCHOT N,et al.Injectable calcium phosphate cement for bone repair and implant fixation.Ortho Clin N Am,2005,36(1):89-95.
[25]PANZAVOLTA S,TORRICELLI P,STURBA L,et al.Setting properties and in vitro bioactivity of strontiumenriched gelatin-calcium phosphate bone cements.J Biomed Mater Res A,2008,84(4):965-972.
[26]CARRODEGUAS RG,DE AZA S.α-tricalcium phosphate:synthesis,properties and biomedical applications.Acta Biomater,2011,7(10):3536-3546.
[27]韩要丛,崔学民,昝青峰,等.α-TCP水化生成羟基磷灰石机理及其应用研究进展.材料导报,2010,24(7):62-66.
[28]田一平,杨建华,李晖,等.影响α-TCP和HA生物陶瓷表面形成类骨磷灰石的形貌、结构的因素研究.四川大学学报(自然科学版),2007,44(1):135-140.
[29]MIYAMOTO Y,ISHIKAWA K,TAKECHI M,et al.Nondecay type fast-setting calcium phosphate cement:setting behaviour in calf serum and its tissue response.Biomaterials,1996,17(14):1429-1435.
[30]史雪婷,徐立新,石宗利.聚磷酸钙纤维增强α-磷酸三钙/纳米羟基磷灰石骨水泥复合材料的力学性能.中国组织工程研究与临床康复,2010,14(51):9535-9538.
[31]ISHIKAWA K,MIYAMOTO Y,KON M,et al.Non-decay type fast-setting calcium phosphate cement:composite with sodium alginate.Biomaterials,1995,16(7):527-532.
[2]MARUKAWA K,UEKI K,OKABE K,et al.Use of selfsettingα-tricalcium phosphate for maxillary sinus augmentation in rabbit.Clin Oral Implants Res,2011,22(6):606-612.
[3]周馨,郑昌琼,王方瑚,等.骨水泥及磷酸钙生物活性骨水泥.硅酸盐通报,1998,17(5):33-38.
[4]ROJBANI H,NYAN M,OHYA K,et al.Evaluation of the osteoconductivity ofα-tricalcium phosphate,β-tricalcium phosphate,and hydroxyapatite combined with or without simvastatin in rat calvarial defect.J Biomed Mater Res A,2011,98(4):488-498.
[5]VARALAKSHMI PR,KAVITHA M,GOVINDAN R,et al.Effect of statins withα-tricalcium phosphate on proliferation,differentiation,and mineralization of human dental pulp cells.J Endod,2013,39(6):806-812.
[6]BIGI A,PANZAVOLTA S,RUBINI K.Relationship between triple-helix content and mechanical properties of gelatin films.Biomaterials,2004,25(25):5675-5680.
[7]TOUNY AH,LAURENCIN C,NAIR L,et al.Formation of composites comprised of calcium deficient HAp and crosslinked gelatin.J Mater Sci:Mater Med,2008,19(10):3193-3201.
[8]KUIJPERS AJ,ENGBERS GH,KRIJGSVELD J,et al.Cross-linking and characteri sterisation of gelatin matrices for biomedical applications.Biomater Sci Polym Ed,2000,11(3):225-243.
[9]MAO JS,CUI YL,WANG XH,et al.A preliminary study on chitosan and gelatin polyelectrolyte complex cytocompatibility by cell cycle and apoptosis analysis.Biomaterials,2004,25(18):3971-3981.
[10]BIGI A,BRACCI B,PANZAVOLTA S.Effect of added gelatin on the properties of calcium phosphate cement.Biomaterials,2004,25(14):2893-2899.
[11]潘朝晖,范清宇,蔡和平,等.明胶对自固化磷酸钙骨水泥性能的影响.医学研究生学报,2005,18(8):696-699.
[12]王彦伟,蔡舒,姚康德.明胶溶胀行为对自固化多孔骨水泥性能和结构的影响.生物骨科材料与临床研究,2003,1(1):41-42.
[13]卫冬娟,张翔,顾建文,等.α-磷酸三钙/HA晶须/羧甲基壳聚糖-明胶复合增强多孔骨水泥的制备研究.生物医学工程学杂志,2012,29(3):491-495.
[14]DOI Y,TAKEZAWA Y,SHIBATA S,et al.Self-setting apatite cement,in physicochemical properties.J Japan Soc Dent Matals Devices,1987(6):53-58.
[15]QU Y,YANG Y,LI J,et al.Preliminary evaluation of a novel strong/osteoinductive calcium phosphate cement.J Biomater Appl,2011,26(3):311.
[16]YUAN H,LI Y,DE BRUIJN JD,et al.Tissue responses of calcium phospliate cement:a study in dogs.Biomaterials,2000,21(12):1283-1290.
[17]PANZAVOLTA S,TORRICELLI P,BRACCI B,et al.Functionalization of biomimetic calcium phosphate bone cements with alendronate.Inorg Biochem,2010,104(10):1099-1106.
[18]戴红莲,闫玉华,李世普,等.α-磷酸三钙-磷酸四钙生物骨水泥的研究.材料科学与工程,2002,20(3):331-335.
[19]LEROUX L,HATIM Z,FRECHE M,et al.Effect of various adjuvants(lactic acid,glycerol and chitosan)on the injectability of a calcium phosphate cement.Bone,1999,25(2Suppl):31S-34S.
[20]MISTRY AS,MIKOS AG.Tissue engineering strategies for bone regeneration.Adv Biochem Eng Biotechnol,2005(94):1-22.
[21]HUTMACHER DW.Scaffolds in tissue engineering bone and cartilage.Biomaterials,2000,21(24):2529-2543.
[22]DURUCAN C,BROWN PW.alpha-tricalcium phosphate hydrolysis to hydroxyapatite at and near physiological temperature.J Mater Sci Mater Med,2000,11(6):365-371.
[23]MONMA H,KANAZAWA T.The hydration ofα-tricalcium phosphate.J Cera Soc Jap,1976,84(4):209-213.
[24]JANSEN J,OOMS E,VERDONSCHOT N,et al.Injectable calcium phosphate cement for bone repair and implant fixation.Ortho Clin N Am,2005,36(1):89-95.
[25]PANZAVOLTA S,TORRICELLI P,STURBA L,et al.Setting properties and in vitro bioactivity of strontiumenriched gelatin-calcium phosphate bone cements.J Biomed Mater Res A,2008,84(4):965-972.
[26]CARRODEGUAS RG,DE AZA S.α-tricalcium phosphate:synthesis,properties and biomedical applications.Acta Biomater,2011,7(10):3536-3546.
[27]韩要丛,崔学民,昝青峰,等.α-TCP水化生成羟基磷灰石机理及其应用研究进展.材料导报,2010,24(7):62-66.
[28]田一平,杨建华,李晖,等.影响α-TCP和HA生物陶瓷表面形成类骨磷灰石的形貌、结构的因素研究.四川大学学报(自然科学版),2007,44(1):135-140.
[29]MIYAMOTO Y,ISHIKAWA K,TAKECHI M,et al.Nondecay type fast-setting calcium phosphate cement:setting behaviour in calf serum and its tissue response.Biomaterials,1996,17(14):1429-1435.
[30]史雪婷,徐立新,石宗利.聚磷酸钙纤维增强α-磷酸三钙/纳米羟基磷灰石骨水泥复合材料的力学性能.中国组织工程研究与临床康复,2010,14(51):9535-9538.
[31]ISHIKAWA K,MIYAMOTO Y,KON M,et al.Non-decay type fast-setting calcium phosphate cement:composite with sodium alginate.Biomaterials,1995,16(7):527-532.