血管内皮生长因子C和皮层肌动蛋白在食管鳞癌细胞中的表达及其临床意义
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摘要
目的血管内皮生长因子C(VEGFC)、皮层肌动蛋白(CTTN)与食管鳞癌的生长密切相关,但其具体作用关系尚未明确。文中旨在通过体外分别干扰人食管鳞癌TE1细胞中VEGFC、CTTN的基因表达,明确两因子对食管鳞癌细胞增殖及凋亡的影响。方法实验根据添加不同试剂分为6组:空白组(单纯培养细胞)、MATE转染组(添加MATE试剂)、阴性对照组(添加阴性对照RNA及MATE试剂)、阳性对照组(添加阳性对照RNA及MATE试剂)、VEGFCsiRNA转染组(添加VEGFC siRNA及MATE试剂)、CTTNsiRNA转染组(添加CTTNsiRNA及MATE试剂)。采用CCK8检测各组细胞增殖情况,流式细胞仪检测细胞凋亡情况。结果 VEGFCsiRNA转染组VEGFC mRNA表达量(0.13±0.01)、CTTNsiRNA转染组CTTN mRNA表达量(0.29±0.02)较空白组(1.00±0.00、1.00±0.00)明显降低(P<0.05)。VEGFCsiRNA转染组、CTTNsiRNA转染组细胞增殖率较其他组明显降低(P<0.05),VEGFCsiRNA转染组细胞增殖率[(31.26±5.25)%]较CTTNsiRNA转染组[(46.99±4.82)%]明显降低(P<0.05)。CTTNsiRNA转染组细胞凋亡率[(36.78±4.29)%]较VEGFCsiRNA转染组[(48.41±5.37)%]明显降低(P<0.05)。结论干扰VEGFC、CTTN表达均可抑制食管鳞癌细胞增殖并促进其凋亡,从而抑制食管鳞癌细胞生长;VEGFC较CTTN对食管鳞癌细胞的增殖及凋亡影响作用更为显著。
Objective Vascular endothelial growth factor C(VEGFC)and cortactin(CTTN)have been found to be closely related to the growth of esophageal squamous cell carcinoma(ESCC),but their specific relationship has not been clearly defined up to the present time. This study aimed to investigate the effects of VEGFC and CTTN on the proliferation and apoptosis of ESCC cells.Methods Human ESCC TE1 cells were treated with normal culture medium(the blank control group),MATE transfection reagent( the MATE group),negative control RNA and MATE reagent(the negative control group),positive control RNA and MATE reagent(the positive control group),VEGFC siRNA and MATE transfection reagent(the VEGFC siRNA group),and CTTN siRNA and MATE transfection reagent(the CTTN siRNA group). The proliferation of the ESCC TE1 cells in different groups was detected by CCK-8 assay and their apoptosis determined by flow cytometry.Results Compared with the blank control group,the ESCC cells of the VEGFC siRNA and CTTN siRNA groups showed significantly decreased expressions of VEGFC mRNA(1.00 ± 0.00 vs 0.13 ± 0.01,P < 0.05)and CTTN mRNA(1.00 ± 0.00 vs 0.29 ± 0.02,P < 0.05). The proliferation rate of the ESCC cells was remarkably lower in the VEGFC siRNA and CTTN siRNA than in the other groups(P < 0.05),and even lower in the VEGFC siRNA than in the CTTN siRNA group([31.26 ± 5.25]% vs[46.99 ± 4.82]%,P < 0.05),but their apoptosis rate was markedly higher in the former than in the latter group([48.41 ± 5.37]% vs[36.78 ± 4.29]%,P < 0.05).Conclusion Interfering with the expressions of VEGFC and CTTN can inhibit the proliferation and promote the apoptosis of ESCC cells,and VEGFC has an even better effect than CTTN.
Objective Vascular endothelial growth factor C(VEGFC)and cortactin(CTTN)have been found to be closely related to the growth of esophageal squamous cell carcinoma(ESCC),but their specific relationship has not been clearly defined up to the present time. This study aimed to investigate the effects of VEGFC and CTTN on the proliferation and apoptosis of ESCC cells.Methods Human ESCC TE1 cells were treated with normal culture medium(the blank control group),MATE transfection reagent( the MATE group),negative control RNA and MATE reagent(the negative control group),positive control RNA and MATE reagent(the positive control group),VEGFC siRNA and MATE transfection reagent(the VEGFC siRNA group),and CTTN siRNA and MATE transfection reagent(the CTTN siRNA group). The proliferation of the ESCC TE1 cells in different groups was detected by CCK-8 assay and their apoptosis determined by flow cytometry.Results Compared with the blank control group,the ESCC cells of the VEGFC siRNA and CTTN siRNA groups showed significantly decreased expressions of VEGFC mRNA(1.00 ± 0.00 vs 0.13 ± 0.01,P < 0.05)and CTTN mRNA(1.00 ± 0.00 vs 0.29 ± 0.02,P < 0.05). The proliferation rate of the ESCC cells was remarkably lower in the VEGFC siRNA and CTTN siRNA than in the other groups(P < 0.05),and even lower in the VEGFC siRNA than in the CTTN siRNA group([31.26 ± 5.25]% vs[46.99 ± 4.82]%,P < 0.05),but their apoptosis rate was markedly higher in the former than in the latter group([48.41 ± 5.37]% vs[36.78 ± 4.29]%,P < 0.05).Conclusion Interfering with the expressions of VEGFC and CTTN can inhibit the proliferation and promote the apoptosis of ESCC cells,and VEGFC has an even better effect than CTTN.
引文
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[19] Pan X,Mao T,Fang W,et al.Vascular endothelial growth factor C is an indicator of lymph node metastasis in thoracic esophageal squamous cellcarcinomas and its role in long-term survival after surgery[J].Thorac Cancer,2014,5(4):313-318.
[20]刘思杰,陈应泰,贾纯增,等.卡培他滨联合参芪扶正注射液对食管癌术后VEGF表达及生活质量的影响[J].现代生物医学进展,2016,16(24):4783-4786.
[21] Zhao G,Kong YL,Wen DQ,et al. Combining cortactin and CTTN detection with clinicopathologic features increases ef-fectiveness of survival predictions for patients with resectable hepatocellular carcinoma[J].Clin Lab,2013,59(11/12):1343-1352.
[22]陈翔,梅广林,王鼎,等.Cortactin及CD166在结肠癌组织中的表达及临床意义[J].实用医学杂志,2011,27(16):2973-2975.
[23]王晓玲.血清VEGF-C含量检测对食管癌临床病理特征、肿瘤恶性程度的评估价值[J].海南医学院学报,2017,23(2):155-157+161.
[24]张九娜.食管鳞癌组织中VEGF-C、CTTN的表达及其临床意义[D].承德医学院,2016.
[25]金艺凤,产翠翠,方蕾,王莹,陈兴无.shRNA干扰沉默ERCC1对A549/DDP细胞增殖与凋亡的影响[J].医学研究生学报,2017,30(6):591-595.
[26]贺婉红,薛嫚,李芳,等.siRNA药物研究进展[J].中国药学杂志,2018,53(14):1145-1151
[2]赵杨,徐树雷,李嘉,等.食管鳞癌中肝细胞生长因子、血管内皮生长因子C及其受体3的表达及意义[J].现代肿瘤医学,2017,25(22):3599-3603.
[3]刘迪,龙谦,罗猛,等. CTTN基因沉默对非小细胞肺癌A549细胞侵袭能力的影响[J].山东医药,2018,58(4):29-31.
[4]郎磊.ATM对乳腺癌细胞迁移和侵袭影响及其机制研究[D].重庆医科大学,2017.
[5]黄子明,张洪义,赵刚,等.肝癌细胞内Sox17基因表达水平与细胞迁移、侵袭力的相关性研究[J].现代肿瘤医学,2012,20(12):2457-2460.
[6]张彤彤,梁建伟,王征,等.Cortactin蛋白在结直肠癌中的表达变化及其临床意义[J].癌变·畸变·突变,2014,26(1):20-23.
[7] Yang H,Wang MR.Molecular mechanisms of CTTN in promoting the invasion and metastasis of esophageal cancer[D].Peking union medical college,2010.
[8] Zheng YP,Chen KS.Effects of esophageal squamous cell carcinoma cells and VEGFC siRNA on proliferation and the ability of lymphangial endothelial cells to form rings[D].Zhengzhou university,2014.
[9] Su CM,Su YH,Chiu CF,et al.Vascular Endothelial Growth Factor-C Upregulates Cortactin and Promotes Metastasis of Esophageal Squamous Cell Carcinoma[J].Ann Surg Oncol,2014,21(4):767-775.
[10]黄清梅,杜小波.食管癌相关标志物研究进展[J].华西医学,2017,32(10):1620-1623.
[11]赵宇,李恒存,朱圣,等.食管癌分子标记物研究进展[J].转化医学电子杂志,2017,4(12):38-44.
[12] Siegel R,Naishadham D,Jemal A.Cancer statistics,2012[J].CA Cancer J Clin,2012,62(1):10.
[13]刘小龙,熊磊,丛壮壮,等.达芬奇机器人在食管癌根治术中学习曲线的研究[J].医学研究生学报,2018,31(8):831-834.
[14]汪春林,黄盛鑫,程小伟,等.VEGF+936C/T多态性与乳腺癌易感性关系的Meta分析[J].肿瘤防治研究,2013,40(5):489-494.
[15]南子晴,吕海宁,胡娅莉.血管内皮生长因子在子宫内膜中作用的研究进展[J].医学研究生学报,2018,31(12):1329-1332.
[16]靳钦,张曙,章建国,等.乳腺癌中血管内皮生长因子和环氧合酶-2蛋白表达与临床因素的关系[J].肿瘤研究与临床,2011,23(9):591.
[17] Lopez-Guerrero AM,Tomas-Martin P,Pascual-Caro C,et al.Regulation of membrane ruffling by polarized STIM1 and ORAI1 in cor-tactin-rich domains[J].Sci Rep,2017,7(1):383.
[18] Meng DF,Xie P,Peng LX,et al.Erratum to:CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by medi-ating invadopodia formation and activating EGFR signaling[J].J Exp Clin Cancer Res,2017,36(1):33.
[19] Pan X,Mao T,Fang W,et al.Vascular endothelial growth factor C is an indicator of lymph node metastasis in thoracic esophageal squamous cellcarcinomas and its role in long-term survival after surgery[J].Thorac Cancer,2014,5(4):313-318.
[20]刘思杰,陈应泰,贾纯增,等.卡培他滨联合参芪扶正注射液对食管癌术后VEGF表达及生活质量的影响[J].现代生物医学进展,2016,16(24):4783-4786.
[21] Zhao G,Kong YL,Wen DQ,et al. Combining cortactin and CTTN detection with clinicopathologic features increases ef-fectiveness of survival predictions for patients with resectable hepatocellular carcinoma[J].Clin Lab,2013,59(11/12):1343-1352.
[22]陈翔,梅广林,王鼎,等.Cortactin及CD166在结肠癌组织中的表达及临床意义[J].实用医学杂志,2011,27(16):2973-2975.
[23]王晓玲.血清VEGF-C含量检测对食管癌临床病理特征、肿瘤恶性程度的评估价值[J].海南医学院学报,2017,23(2):155-157+161.
[24]张九娜.食管鳞癌组织中VEGF-C、CTTN的表达及其临床意义[D].承德医学院,2016.
[25]金艺凤,产翠翠,方蕾,王莹,陈兴无.shRNA干扰沉默ERCC1对A549/DDP细胞增殖与凋亡的影响[J].医学研究生学报,2017,30(6):591-595.
[26]贺婉红,薛嫚,李芳,等.siRNA药物研究进展[J].中国药学杂志,2018,53(14):1145-1151