黄芪注射液对Balb/c裸鼠银屑病模型的皮损程度及Caspase-14、SOCS1、STAT3水平的影响
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摘要
目的:研究黄芪注射液对Balb/c裸鼠银屑病模型的皮损程度及Caspase-14、SOCS1、STAT3水平的影响。方法:选取60只Balb/c裸鼠随机数字表分为A、B、C 3组,每组各20只,A组小鼠作为空白对照,B组小鼠作为模型组,C组小鼠作为治疗组。3组小鼠均治疗2周,分析治疗后3组小鼠银屑病PASI评分、小鼠病损皮肤厚度、炎性因子、血清Caspase-14、SOCS1、STAT3水平对比之间的差异。结果:经过治疗后,B组患者的PASI评分显著高于C组,差异具有统计学意义(P<0.05);3组小鼠的治疗后病损皮肤测量比较,差异有统计学意义(P<0.05),通过两两比较,相比空白对照组,B、C两组小鼠的病损皮肤厚度均显著升高,且治疗组小鼠的病损皮肤厚度显著低于模型组,差异具有统计学意义(P<0.05);3组小鼠的治疗后炎性因子之间比较,差异有统计学意义(P<0.05),通过两两比较,相比空白对照组,B、C两组小鼠的炎性因子IL-17、IL-22、 IL-23均显著升高,且治疗组小鼠的炎性因子IL-17、IL-22、 IL-23显著低于模型组,差异有统计学意义(P<0.05);3组小鼠的治疗后血清Caspase-14、SOCS1、STAT3水平比较,差异有统计学意义(P<0.05),通过两两比较,相比空白对照组,B、C两组小鼠的炎性因子血清Caspase-14、SOCS1水平均显著降低,STAT3水平显著升高,且治疗组小鼠的炎性因子aspase-14、SOCS1水平显著低于模型组,STAT3水平显著高于模型组,差异有统计学意义(P<0.05)。结论:黄芪注射液对Balb/c裸鼠银屑病模型的皮损程度有效改善,其可能机制认为是通过对Caspase-14、SOCS1表达水平的降低,降低小鼠的皮损部位的角化程度,改善局部表层增生情况,同时提升STAT3水平,增强局部细胞增殖水平,对于银屑病的康复具有积极意义。
Objective: To investigate the effect of astragalus injection on the skin lesion degree and caspase-14, SOCS1 and STAT3 levels of psoriasis model in balb/c nude mice. Methods: Sixty Balb/c nude mice were randomly divided into groups A, B and C with 20 mice in each group. Group A mice were used as blank control, group B mice as model group and group C mice as treatment group. The PASI score of psoriasis, skin thickness, inflammatory factors, serum levels of Caspase-14, SOCS1 and STAT3 in three groups of mice were analyzed after 2 weeks of treatment. Result:After treatment, the P ASI score of group B was significantly higher than that of group C, with statistical significance(P<0.05); there was statistical significance in the measurements of lesion skin of three groups of mice after treatment(P<0.05). Compared with the blank control group, the thickness of lesion skin in group B and C was significantly higher, and the thickness of lesion skin in treatment group was significantly lower than that in control group(P<0.05). Compared with the blank control group, the inflammatory factors IL-17, IL-22 and IL-23 in the B and C groups were significantly increased, and the inflammatory factors IL-17, IL-22 and IL-23 in the treatment group were significantly lower than those in the model group. The levels of serum C aspase-14, SOCS1 and STAT3 in three groups of mice were significantly different after treatment(P<0.05). Compared with the blank control group, the levels of serum C aspase-14 and SOCS1 in B and C groups were significantly lower and the levels of STAT3 were significantly higher, and the levels of inflammatory factors aspase-14 and SO in treatment group were significantly higher than those in control group. The level of CS1 was significantly lower than that of model group, and the level of STAT3 was significantly higher than that of model group(P<0.05).Conclusion:Astragalus membranaceus injection can effectively improve the degree of psoriasis in Balb/c nude mice. Its possible mechanism is that it can decrease the expression of Caspase-14 and SOCS1, reduce the degree of keratosis in the lesion site of mice, improve the local surface hyperplasia, increase the level of STAT3 and enhance the level of local cell proliferation, which is of positive significance for the rehabilitation of psoriasis.
Objective: To investigate the effect of astragalus injection on the skin lesion degree and caspase-14, SOCS1 and STAT3 levels of psoriasis model in balb/c nude mice. Methods: Sixty Balb/c nude mice were randomly divided into groups A, B and C with 20 mice in each group. Group A mice were used as blank control, group B mice as model group and group C mice as treatment group. The PASI score of psoriasis, skin thickness, inflammatory factors, serum levels of Caspase-14, SOCS1 and STAT3 in three groups of mice were analyzed after 2 weeks of treatment. Result:After treatment, the P ASI score of group B was significantly higher than that of group C, with statistical significance(P<0.05); there was statistical significance in the measurements of lesion skin of three groups of mice after treatment(P<0.05). Compared with the blank control group, the thickness of lesion skin in group B and C was significantly higher, and the thickness of lesion skin in treatment group was significantly lower than that in control group(P<0.05). Compared with the blank control group, the inflammatory factors IL-17, IL-22 and IL-23 in the B and C groups were significantly increased, and the inflammatory factors IL-17, IL-22 and IL-23 in the treatment group were significantly lower than those in the model group. The levels of serum C aspase-14, SOCS1 and STAT3 in three groups of mice were significantly different after treatment(P<0.05). Compared with the blank control group, the levels of serum C aspase-14 and SOCS1 in B and C groups were significantly lower and the levels of STAT3 were significantly higher, and the levels of inflammatory factors aspase-14 and SO in treatment group were significantly higher than those in control group. The level of CS1 was significantly lower than that of model group, and the level of STAT3 was significantly higher than that of model group(P<0.05).Conclusion:Astragalus membranaceus injection can effectively improve the degree of psoriasis in Balb/c nude mice. Its possible mechanism is that it can decrease the expression of Caspase-14 and SOCS1, reduce the degree of keratosis in the lesion site of mice, improve the local surface hyperplasia, increase the level of STAT3 and enhance the level of local cell proliferation, which is of positive significance for the rehabilitation of psoriasis.
引文
1 董颖颖, 钟世玉, 王琼, 等. 银屑病小鼠模型建立的新方法[J].中国皮肤性病学杂志, 2016,(11):1127-1131.
2 孙文, 陈雨佳, 邓婉莹,等.银屑I号对咪喹莫特诱导小鼠银屑病模型核转录因子-κB的调控作用及机制[J].中国皮肤性病学杂志, 2017,(1):88-90,103.
3 朱超英, 温炬, 李婷, 等. IL-36α对银屑病小鼠皮肤病变的影响及其作用机制[J]. 山东医药, 2017,(28):43-45.
4 李小娜, 张亚梅, 许江南. STAT1和T-bet基因敲除在咪喹莫特诱导的小鼠银屑病模型中的不同作用[J].免疫学杂志, 2016,(8):687-691.
5 王苹, 唐燕. 银屑病PASI评分系统的设计与实现[J].中国医药导报, 2017, 14(15):179-182.
6 李雪,解欣然,李宁飞,等.凉血解毒利湿方对小鼠银屑病样皮损中过氧化物酶体增殖激活受体的影响[J].中医杂志,2017,58(23):2032-2038,2073.
7 李鹏英, 郭菲, 丰靓, 等. 健脾解毒汤对小鼠银屑病模型作用的实验研究[J]. 中国临床药理学杂志, 2017(20):68-70.
8 Calautti E, Avalle L, Poli V. Psoriasis: A STAT3-Centric View[J]. International Journal of Molecular Sciences, 2018, 19(1):171.
9 Gao G, Fan H, Zhang X, et al. Neuroprotective effect of G(14)-humanin on global cerebral ischemia/reperfusion by activation of SOCS3 - STAT3 - MCL-1 signal transduction pathway in rats.[J]. Neurological Research, 2017, 39(5):1.
10 Yang L , Li B , Dang E , et al. Impaired function of regulatory T cells in patients with psoriasis is mediated by phosphorylation of STAT3[J]. Journal of Dermatological Science, 2016, 81(2):85-92.
11 宋新志. 黄芪注射液治疗银屑病患者的临床观察和实验研究[J]. 医学信息, 2016, 29(2):36-37.
12 Madonna S, Scarponi C, Morelli M, et al. SOCS3 inhibits the pathological effects of IL-22 in non-melanoma skin tumor-derived keratinocytes[J]. Oncotarget, 2017, 8(15):24652-24667.
13 曾凡杞, 廖明, 张志云,等. 腺病毒介导的SOCS1对模拟银屑病小鼠模型的影响[J]. 华中科技大学学报(医学版), 2014,43(2):224-228.
14 Qin X, Chen C, Zhang Y, et al. Acitretin modulates HaCaT cells proliferation through STAT1- and STAT3-dependent signaling[J]. Saudi Pharmaceutical Journal, 2017, 25(4):620-624.
15 Cekic C, Linden J. Purinergic regulation of the immune system[J]. Nature Reviews Immunology, 2016, 16(3):177-192.
2 孙文, 陈雨佳, 邓婉莹,等.银屑I号对咪喹莫特诱导小鼠银屑病模型核转录因子-κB的调控作用及机制[J].中国皮肤性病学杂志, 2017,(1):88-90,103.
3 朱超英, 温炬, 李婷, 等. IL-36α对银屑病小鼠皮肤病变的影响及其作用机制[J]. 山东医药, 2017,(28):43-45.
4 李小娜, 张亚梅, 许江南. STAT1和T-bet基因敲除在咪喹莫特诱导的小鼠银屑病模型中的不同作用[J].免疫学杂志, 2016,(8):687-691.
5 王苹, 唐燕. 银屑病PASI评分系统的设计与实现[J].中国医药导报, 2017, 14(15):179-182.
6 李雪,解欣然,李宁飞,等.凉血解毒利湿方对小鼠银屑病样皮损中过氧化物酶体增殖激活受体的影响[J].中医杂志,2017,58(23):2032-2038,2073.
7 李鹏英, 郭菲, 丰靓, 等. 健脾解毒汤对小鼠银屑病模型作用的实验研究[J]. 中国临床药理学杂志, 2017(20):68-70.
8 Calautti E, Avalle L, Poli V. Psoriasis: A STAT3-Centric View[J]. International Journal of Molecular Sciences, 2018, 19(1):171.
9 Gao G, Fan H, Zhang X, et al. Neuroprotective effect of G(14)-humanin on global cerebral ischemia/reperfusion by activation of SOCS3 - STAT3 - MCL-1 signal transduction pathway in rats.[J]. Neurological Research, 2017, 39(5):1.
10 Yang L , Li B , Dang E , et al. Impaired function of regulatory T cells in patients with psoriasis is mediated by phosphorylation of STAT3[J]. Journal of Dermatological Science, 2016, 81(2):85-92.
11 宋新志. 黄芪注射液治疗银屑病患者的临床观察和实验研究[J]. 医学信息, 2016, 29(2):36-37.
12 Madonna S, Scarponi C, Morelli M, et al. SOCS3 inhibits the pathological effects of IL-22 in non-melanoma skin tumor-derived keratinocytes[J]. Oncotarget, 2017, 8(15):24652-24667.
13 曾凡杞, 廖明, 张志云,等. 腺病毒介导的SOCS1对模拟银屑病小鼠模型的影响[J]. 华中科技大学学报(医学版), 2014,43(2):224-228.
14 Qin X, Chen C, Zhang Y, et al. Acitretin modulates HaCaT cells proliferation through STAT1- and STAT3-dependent signaling[J]. Saudi Pharmaceutical Journal, 2017, 25(4):620-624.
15 Cekic C, Linden J. Purinergic regulation of the immune system[J]. Nature Reviews Immunology, 2016, 16(3):177-192.