鳀鱼多肽-铜离子螯合工艺优化及其影响因素探索
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摘要
以鳀鱼为原料,研究多肽与铜离子螯合的影响因素。通过比较在不同多肽与硫酸铜添加比例、pH值、温度和时间条件下的螯合率及进行正交试验,确定最佳工艺条件,并在最佳条件下探索多肽分子量对螯合率的影响。结果表明,最佳螯合条件为多肽与硫酸铜的添加比例6∶1、pH 6、温度50℃、时间50 min,在此条件下螯合率为61.3%。多肽分子量小于300 u时螯合率较低,在(600～1 000) u时螯合率最高为72.6%,大于1 000 u时,铜离子螯合率下降。
The anchovy was used as raw material to study the influencing factors of peptide chelation with copper ions. Comparing the chelate rate under different conditions,such as the addition ratio of polypeptide and copper sulfate,pH,reaction temperature and time, the optimal process conditions were determined through orthogonal test.And the effect of molecular weight on chelate rate was explored under optimal conditions.The result showed that the optimal chelation process was the addition ratio of 6∶1,pH6,reaction for 50 min at 50℃,and the chelate rate was 61.3% under these conditions.The chelate rate was lower when the molecular weight of the peptide was less than 300 u.When the molecular weight of the peptide was between 600 and 1 000 u,the chelate rate was the highest as 72.6%.When the peptide was larger than 1 000 u,the chelate rate of copper ions decreased.
The anchovy was used as raw material to study the influencing factors of peptide chelation with copper ions. Comparing the chelate rate under different conditions,such as the addition ratio of polypeptide and copper sulfate,pH,reaction temperature and time, the optimal process conditions were determined through orthogonal test.And the effect of molecular weight on chelate rate was explored under optimal conditions.The result showed that the optimal chelation process was the addition ratio of 6∶1,pH6,reaction for 50 min at 50℃,and the chelate rate was 61.3% under these conditions.The chelate rate was lower when the molecular weight of the peptide was less than 300 u.When the molecular weight of the peptide was between 600 and 1 000 u,the chelate rate was the highest as 72.6%.When the peptide was larger than 1 000 u,the chelate rate of copper ions decreased.
引文
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[11] 许庆陵,王玉飞,刘浩怀,等.罗非鱼多肽-Ca、Cu螯合工艺及其产物的结构特征[J].食品科技,2015,40(11):108-112.
[12] 刘永,黎彬庆,韦寿莲.罗非鱼鳞胶原蛋白肽锌螯合物制备工艺优[J].食品与发酵工业,2013,39(4):125-129.
[13] 杨燊,邓尚贵,秦小明.低值鱼蛋白多肽-钙螯合物的制备和抗氧化、抗菌活性研究[J].食品科学,2008,29(1):202-206.
[14] HITOMI K,YOSHIHARU S.Influence of phytate removal and structure modification on calcium-binging pproperties of soybean globulins[J]. Biotechnol Biochem,1998,62:341-346.
[15] 付文雯.牛骨胶原多肽螯合釣的制备及其结构表征[D].武汉:华中农业大学,2010.
[16] BAO X L,LV Y,YANG B C, et al.A study of the soluble complexes formed during calcium bingding by soybean protein hydrolysates[J]. Food Chemistry,2008(10):1 740-1 841.
[2] SIRINGAN P,RAKSAKULTHAI N,YONGSAWATDIGUL J.Partial purification and characterization of trypsin-like proteinases in Indianan chovy[J].Food Chemistry,2007,11(49):82-89.
[3] HEU M S,KIM H R,CHO D M.Purification and characterization of cathepsin L-like enzyme from the muscle of anchovy,engraulis japonica[J].Elsevier,1997,118(3):523-529.
[4] SIRINGAN P,RAKSAKULTHAI N,YONGSAWATDIGUL J.Autolytic activity and biochemical characteristics of endogenous proteinases in Indian anchovy (Stolephorus indicus)[J] .Food Chemistry,2006,98:678-684.
[5] HARRIS Z L,TAKAHASHI Y,MIYAJIMA H.Aceruloplasminemia:molecular characterization of this disorder of iron metabolism[J].Proceedings of the Nationa Academy of Sciences,USA 1995,92:2 539-2 543.
[6] ARREDONDO M,NUNEZ M T.Iron and copper metabolism[J].Molecular Aspects of Medicine,2005,26:313-327.
[7] AUCHI K,WAKAMATSU K,ITO S.An oxygen transporter hemocyanin can act on the late pathway of melanin synthesis[J].Pigment Cell Research,2005,19:214-219.
[8] 刘温楼,乔明,杨文鸽.多肽金属元素螯合物研究进展[J].食品与发酵工业,2014(4):148-152.
[9] 滕冰,舒绪刚.微量元素氨基酸螯合物的研究进展:饲料科学技术交流大会论文集[C].济南:山东畜牧兽医,2007.
[10] 陈晓刚,陈忻.鳕鱼皮胶原蛋白肽铜螯合物的制备条件[J].贵州农业科学,2015,43(1):154-158.
[11] 许庆陵,王玉飞,刘浩怀,等.罗非鱼多肽-Ca、Cu螯合工艺及其产物的结构特征[J].食品科技,2015,40(11):108-112.
[12] 刘永,黎彬庆,韦寿莲.罗非鱼鳞胶原蛋白肽锌螯合物制备工艺优[J].食品与发酵工业,2013,39(4):125-129.
[13] 杨燊,邓尚贵,秦小明.低值鱼蛋白多肽-钙螯合物的制备和抗氧化、抗菌活性研究[J].食品科学,2008,29(1):202-206.
[14] HITOMI K,YOSHIHARU S.Influence of phytate removal and structure modification on calcium-binging pproperties of soybean globulins[J]. Biotechnol Biochem,1998,62:341-346.
[15] 付文雯.牛骨胶原多肽螯合釣的制备及其结构表征[D].武汉:华中农业大学,2010.
[16] BAO X L,LV Y,YANG B C, et al.A study of the soluble complexes formed during calcium bingding by soybean protein hydrolysates[J]. Food Chemistry,2008(10):1 740-1 841.