滑鼠蛇肾脏组织形态结构
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摘要
探索滑鼠蛇肾脏的组织结构与特征,为其人工养殖和野生保护提供理论依据。试验通过常规大体解剖方法、组织石蜡制片技术和苏木精-伊红(H-E)染色技术,对滑鼠蛇肾脏的组织形态结构进行研究。结果表明:滑鼠蛇的肾脏由被膜与实质构成,被膜由内、外两层构成,实质包括大量的泌尿小管和少量的结缔组织。泌尿小管包括肾单位与集合小管,肾单位中的肾小体平均直径285.107μm,肾小囊内外层间距平均31.063μm;在肾小体周围的远端小管和近端小管分布紧密,近端小管的管壁厚平均16.275μm,远端小管的管壁厚平均15.245μm,集合小管的管壁厚平均26.937 6μm。滑鼠蛇的肾小体过滤功能较弱,肾小管浓缩尿液功能较强,排泄尿液量少,其肾组织形态学结构有明显的爬行类动物特征。
The object is to observe the tissue structure and property,determine the physiological function of the kidneys of Ptyas mucosus,and provide theoretical basics for the artificial breeding and wildlife conservation. The morphological structure of the kidney was analyzed by hematoxylin-eosin( H-E) staining. The results showed that: the kidney consisted of two layers of capsule and the parenchyma containing lot of uriniferous tubule and few of connective tissue. The uriniferous tubule consisted of nephron and collecting tubule. The average diameter of the renal corpuscles of nephron was about 285.107 μm. The average distance between the inner and outer layers of renal capsule was31.063 μm The distal and proximal tubules around the renal corpuscles were closely distributed,the average wall thickness of proximal tubules was 16.275 μm,the average wall thickness of distal tubules was 15.245 μm,the average wall thickness of collecting tubule was 26.937 6 μm. The filtration function of Ptyas mucosus corpuscles was weak. The function of renal tubule concentrated urine was larger,so it had less excretion of urine. The histomorphological structure of the kidney had a distinct reptilian feature.
The object is to observe the tissue structure and property,determine the physiological function of the kidneys of Ptyas mucosus,and provide theoretical basics for the artificial breeding and wildlife conservation. The morphological structure of the kidney was analyzed by hematoxylin-eosin( H-E) staining. The results showed that: the kidney consisted of two layers of capsule and the parenchyma containing lot of uriniferous tubule and few of connective tissue. The uriniferous tubule consisted of nephron and collecting tubule. The average diameter of the renal corpuscles of nephron was about 285.107 μm. The average distance between the inner and outer layers of renal capsule was31.063 μm The distal and proximal tubules around the renal corpuscles were closely distributed,the average wall thickness of proximal tubules was 16.275 μm,the average wall thickness of distal tubules was 15.245 μm,the average wall thickness of collecting tubule was 26.937 6 μm. The filtration function of Ptyas mucosus corpuscles was weak. The function of renal tubule concentrated urine was larger,so it had less excretion of urine. The histomorphological structure of the kidney had a distinct reptilian feature.
引文
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[16]岳兴建,张耀光,敖磊,等.南方鲇泌尿系统的结构[J].西南农业大学学报,2007,29(4):137-143.
[17]林华英,姜明.不同生境中鲈鱼肾脏显微和亚微结构变化的初步研究[J].山东海洋学院学报,1985,15(4):64-70.
[18]温龙岚,石禹,王志坚.西藏墨头鱼泌尿系统结构[J].重庆师范大学学报,2008,25(4):8-11.
[19]区又君,陈四海,李加儿.珠江口池养梭鱼肾脏显微和超微结构观察[J].生态科学,2014,33(4):686-690.
[2]赵晓华,严若谷,李其斌.养殖与野生滑鼠蛇蛇肉营养成分比较分析[J].蛇志,2013,25(1):13-14,86.
[3]付贞,胡明玥,麦金辉,等.滑鼠蛇肺脏组织结构[J].经济动物学报,2018,22(4):217-220.
[4]胡明玥,武东怡,付贞,等.滑鼠蛇胃肠道组织结构[J].经济动物学报,2018,22(4):221-224.
[5]谭超,刘进辉,王水莲,等.东北虎肾组织形态结构的研究[J].经济动物学报,2003,7(1):35-37.
[6]辛志光.家畜组织与胚胎学[M].北京:农业出版社,1992:156-162.
[7]徐春生.中华鳖肾组织的比较细胞学研究及其ACE2的分布与基因克隆[D].南京:南京农业大学,2010:14-43.
[8] Braun E J. Comparative renal function in reptiles,birds and mammals[J]. Seminars in Avian and Exotic Pet Medicine,1988,7(98):62-71.
[9] Maluf N S R.Future studies on the kidney of the hook-lipped African rhinoceros,Diceros bicornis[J]. Anat Rec,1994,238(1):38-48.
[10]王丽敏,邢向阳,杨纯.秦岭滑蜥排泄系统组织形态学观察[J].四川动物,2015,34(3):389-393.
[11] Tsujii T,Inoue S,Takamiya H,et al.Morphology of the kidney of the platypus(Ornithorhynchus anatinus:Monotremata)[J].Anat Rec,1992,234(3):348-358.
[12]逯志强.非洲白犀牛肾脏的组织学研究[C]∥中国畜牧兽医学会动物解剖学与组织胚胎学分会.中国畜牧兽医学会动物解剖学及组织胚胎学分会第十六次学术研讨会论文集.北京:中国畜牧兽医学会,2010:285-286.
[13]林国辉,邱玫,方展强.剑尾鱼肾脏组织的超微结构观察[J].华南师范大学学报,2011,12(1):105-108.
[14]蔡文超,区又君.驼背鲈肾脏显微和超微结构观察[J].中国水产科学,2008,15(5):773-778.
[15]车静,张耀光,岳兴建,等.食蚊鱼泌尿系统结构[J].西南农业大学学报,2002,24(1):50-53.
[16]岳兴建,张耀光,敖磊,等.南方鲇泌尿系统的结构[J].西南农业大学学报,2007,29(4):137-143.
[17]林华英,姜明.不同生境中鲈鱼肾脏显微和亚微结构变化的初步研究[J].山东海洋学院学报,1985,15(4):64-70.
[18]温龙岚,石禹,王志坚.西藏墨头鱼泌尿系统结构[J].重庆师范大学学报,2008,25(4):8-11.
[19]区又君,陈四海,李加儿.珠江口池养梭鱼肾脏显微和超微结构观察[J].生态科学,2014,33(4):686-690.