一步法水热合成枸杞荧光碳点及对Fe~(3+)的灵敏检测
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摘要
以枸杞为碳源,采用简单、环境友好的一步水热法合成未经表面修饰的荧光碳点。采用紫外-可见吸收光谱、荧光光谱、高分辨透射电子显微镜、傅里叶变换红外光谱和X射线光电子能谱对其性质及形貌进行了研究。在最佳条件下,碳点的激发峰和发射峰分别位于320和400 nm,且发射峰具有尺寸依赖的性质。以罗丹明6G为参照,此碳点的量子产率54%,荧光寿命9.04μs。碳点的荧光在2 min内被Fe~(3+)完全猝灭。基于此,构建了检测Fe~(3+)的新型荧光生物传感器。在1~50μmol/L范围内,荧光猝灭程度与Fe~(3+)的浓度呈良好的线性关系,检出限为232 nmol/L。此碳点在环境和生物医学领域具有良好的应用前景。
Water dispersible fluorescent carbon nanodots( CDs) were prepared by a facile and eco-friendly hydrothermal process using Chinese wolfberry as precursor without any further surface modification. The obtained CDs were characterized by UV-visible absorption spectroscopy,fluorescence spectroscopy,high-resolution transmission electron microscopy,Fourier transform infrared and X-ray photoelectron spectroscopy.It showed emission spectrum of blue which located at 400 nm with excited wavelength of 320 nm. High quantum yield of 54%,long lifetime of 9.04 μs,and good biocompatibility were also observed. Importantly,the fluorescence intensity of CDs was selectively quenched within 2 min after addition of Fe~(3+). Based upon the phenomenon,a new fluorescent biosensor for detection of Fe~(3+)was proposed. No significant interference from other metal ions was observed. The linear range and detection limit for Fe~(3+) was 1-50 μmol/L and 232 nmol/L( 3σ), respectively. These superior properties demonstrated that the synthesized CDs had promising applications in the fields of environmental and biomedical science.
Water dispersible fluorescent carbon nanodots( CDs) were prepared by a facile and eco-friendly hydrothermal process using Chinese wolfberry as precursor without any further surface modification. The obtained CDs were characterized by UV-visible absorption spectroscopy,fluorescence spectroscopy,high-resolution transmission electron microscopy,Fourier transform infrared and X-ray photoelectron spectroscopy.It showed emission spectrum of blue which located at 400 nm with excited wavelength of 320 nm. High quantum yield of 54%,long lifetime of 9.04 μs,and good biocompatibility were also observed. Importantly,the fluorescence intensity of CDs was selectively quenched within 2 min after addition of Fe~(3+). Based upon the phenomenon,a new fluorescent biosensor for detection of Fe~(3+)was proposed. No significant interference from other metal ions was observed. The linear range and detection limit for Fe~(3+) was 1-50 μmol/L and 232 nmol/L( 3σ), respectively. These superior properties demonstrated that the synthesized CDs had promising applications in the fields of environmental and biomedical science.
引文
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2 Miao P,Han K,Tang Y,Wang B,Lin T,Cheng W.Nanoscale,2015,7(5):1586-1595
3 Shu Y,Lu J,Mao Q,Song R,Wang X,Chen X,Wang J.Carbon,2017,114:324-333
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11 Liu S,Tian J,Wang L,Zhang Y,Qin X,Luo Y,Asiri A M,Al-Youbi A O,Sun X.Adv.Mater.,2012,24(15):2037-2041
12 Lu W,Qin X,Liu S,Chang G,Zhang Y,Luo Y,Asiri A M,Al-Youbi A O,Sun X.Anal.Chem.,2012,84(12):5351-5357
13 Wang W,Ni Y,Xu Z.J.Alloy Compd.,2015,622:303-308
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