山黄粉和黄芪多糖配伍使用对产蛋鸡生产性能、蛋品质、血清抗氧化和生化指标的影响
|
摘要
本试验旨在研究山黄粉(FLBS)和黄芪多糖(APS)配伍使用对产蛋鸡生产性能、蛋品质、血清抗氧化和生化指标的影响。试验选取43周龄产蛋率和体重相近的健康海兰褐蛋鸡432只,随机分成4个组,每组6个重复,每个重复18只鸡。对照组(Ⅰ组)饲喂基础饲粮,试验组分别在基础饲粮的基础上添加100 mg/kg FLBS+100 mg/kg APS (Ⅱ组)、100 mg/kg FLBS+200 mg/kg APS(Ⅲ组)、100 mg/kg FLBS+400 mg/kg APS(Ⅳ组)。预试期1周,正试期12周。结果表明:1)与对照组相比,Ⅱ、Ⅲ和Ⅳ组产蛋鸡生产性能和蛋品质指标均无显著变化(P>0.05)。2)与对照组相比,Ⅲ和Ⅳ组试验第6和12周末的血清总抗氧化能力(T-AOC)显著提高(P<0.05);试验第12周末,Ⅲ和Ⅳ组的血清丙二醛(MDA)含量显著降低(P<0.05),Ⅱ、Ⅲ和Ⅳ组的血清超氧化物歧化酶(SOD)活性显著提高(P<0.05)。3)与对照组相比,试验第6周末,Ⅱ组的血清尿酸(UA)含量显著升高(P<0.05),Ⅱ和Ⅲ组的血清总胆固醇(TC)含量显著降低(P<0.05);试验第12周末,Ⅱ、Ⅲ和Ⅳ组的血清UA和甘油三酯(TG)含量显著降低(P<0.05),Ⅱ和Ⅳ组的血清葡萄糖(GLU)含量显著提高(P<0.05)。由此可见,饲粮中FLBS和APS配伍使用可增强蛋鸡机体抗氧化能力,并在脂质代谢、肝肾功能上都具有一定程度的改善作用。本试验中,100 mg/kg FLBS+100或200 mg/kg APS组效果较好。
This experiment was conducted to investigate the effects of Flos lonicerae-Baikal skullcap (FLBS)and Astragalus polysacharin (APS) compatibility use on performance,egg quality,serum antioxidant and biochemical indexes of laying hens. Four hundred and thirty-two healthy Hy-Line Grey laying hens at 43 weeks old were randomly allotted into 4 groups with 6 replicates per group and 18 hens per replicate. Hens in the control group (group Ⅰ) were fed a basal diet,and the others in experimental groups were fed the basal diet supplemented with 100 mg/kg FLBS +100 mg/kg APS (group Ⅱ),100 mg/kg FLBS +200 mg/kg APS (groupⅢ) and 100 mg/kg FLBS+400 mg/kg APS (group Ⅳ). The adaptation period lasted for 1 week and the formal period lasted for 12 weeks. The results showed as follows: 1) compared with the control group,the indexes of performance and egg quality of laying hens had no significant changes (P > 0. 05). 2) Compared with the control group,the serum total antioxidant capacity (T-AOC) of groups Ⅲ and Ⅳ at the 6 th and 12 th weekend of the experiment was significantly increased (P<0.05); at the 12 th weekend of the experiment,the serum malondialdehyde (M DA) content of groups Ⅲ and Ⅳ was significantly decreased (P<0.05),the serum superoxide dismutase (SOD) activity of groups Ⅱ,Ⅲ and Ⅳ was significantly increased (P < 0.05). 3)Compared with the control group,at the 6 th weekend of the experiment,the serum uric acid (UA) content of group Ⅱ was significantly increased (P<0.05),the serum total cholesterol (TC) content of groups Ⅱ and Ⅲwas significantly decreased (P<0.05); at the 12 th weekend of the experiment,the serum UA and triglyceride (TG) contents of groups Ⅱ,Ⅲ and Ⅳ were significantly decreased (P<0.05),the serum glucose (GLU)content of groups Ⅱ and Ⅳ was significantly increased (P<0.05). In conclusion,dietary FLBS and APS compatibility use can increase the antioxidant ability of laying hens,also has a certain degree of improvement on lipid metabolism,liver and kidney function. In this experiment,the 100 mg/kg FLBS +100 or 200 mg/kg APS groups have better effects.
This experiment was conducted to investigate the effects of Flos lonicerae-Baikal skullcap (FLBS)and Astragalus polysacharin (APS) compatibility use on performance,egg quality,serum antioxidant and biochemical indexes of laying hens. Four hundred and thirty-two healthy Hy-Line Grey laying hens at 43 weeks old were randomly allotted into 4 groups with 6 replicates per group and 18 hens per replicate. Hens in the control group (group Ⅰ) were fed a basal diet,and the others in experimental groups were fed the basal diet supplemented with 100 mg/kg FLBS +100 mg/kg APS (group Ⅱ),100 mg/kg FLBS +200 mg/kg APS (groupⅢ) and 100 mg/kg FLBS+400 mg/kg APS (group Ⅳ). The adaptation period lasted for 1 week and the formal period lasted for 12 weeks. The results showed as follows: 1) compared with the control group,the indexes of performance and egg quality of laying hens had no significant changes (P > 0. 05). 2) Compared with the control group,the serum total antioxidant capacity (T-AOC) of groups Ⅲ and Ⅳ at the 6 th and 12 th weekend of the experiment was significantly increased (P<0.05); at the 12 th weekend of the experiment,the serum malondialdehyde (M DA) content of groups Ⅲ and Ⅳ was significantly decreased (P<0.05),the serum superoxide dismutase (SOD) activity of groups Ⅱ,Ⅲ and Ⅳ was significantly increased (P < 0.05). 3)Compared with the control group,at the 6 th weekend of the experiment,the serum uric acid (UA) content of group Ⅱ was significantly increased (P<0.05),the serum total cholesterol (TC) content of groups Ⅱ and Ⅲwas significantly decreased (P<0.05); at the 12 th weekend of the experiment,the serum UA and triglyceride (TG) contents of groups Ⅱ,Ⅲ and Ⅳ were significantly decreased (P<0.05),the serum glucose (GLU)content of groups Ⅱ and Ⅳ was significantly increased (P<0.05). In conclusion,dietary FLBS and APS compatibility use can increase the antioxidant ability of laying hens,also has a certain degree of improvement on lipid metabolism,liver and kidney function. In this experiment,the 100 mg/kg FLBS +100 or 200 mg/kg APS groups have better effects.
引文
[1]龙彬,李周权,董国忠,等.金银花提取物对蛋鸡生产性能、蛋品质、脂质代谢及蛋黄胆固醇含量的影响[J].动物营养学报,2018,30(1):212-218.
[2]孙先枝,程建波,卜登攀,等.黄芩苷的生物学功能和黄芩及其提取物在畜禽生产中的应用研究进展[J].动物营养学报,2013,25(7):1459-1464.
[3]LI S P,ZHAO X J,WANG J Y.Synergy of Astragalus polysaccharides and probiotics(Lactobacillus and Bacillus cereus)on immunity and intestinal microbiota in chicks[J].Poultry Science,2009,88(3):519-525.
[4]官丽辉,张立永,刘海斌,等.黄芪多糖对蛋鸡生产性能、生殖激素及血液生理生化指标的影响[J].中国粮油学报,2015,30(7):70-76.
[5]李振.中草药抗热应激剂对蛋鸡生产性能、蛋品质及血液生化指标的影响[J].西北农林科技大学学报(自然科学版),2011,39(9):48-52.
[6]CHEN H L,LI D F,CHANG B Y,et al.Effects of Chinese herbal polysaccharides on the immunity and grow th performance of young broilers[J].Poultry Science,2003,82(3):364-370.
[7]WANG H F,YANG W R,YANG H W,et al.Effects of Astragalus membranaceus on grow th performance,carcass characteristics,and antioxidant status of broiler chickens[J].Acta Agriculturae Scandinavica,Section A:Animal Science,2010,60(3):151-158.
[8]王宇,周选武,陈代文,等.绿原酸对仔猪生长性能、血清免疫球蛋白及肠道黏膜形态与消化吸收能力的影响[J].动物营养学报,2018,30(3):1136-1145.
[9]刘青翠,彭翔,张俊平,等.杜仲叶提取物对产蛋后期蛋鸡生产性能、蛋品质、蛋黄胆固醇含量及血清抗氧化指标的影响[J].动物营养学报,2018,30(1):284-292.
[10]WANG Y Z,YANG W R,WANG Y,et alEffects of Astragalus membranaceus on laying performance and antioxidant status of laying hens[J].The Journal of Applied Poultry Research,2012,21(2):243-250.
[11]王洪芳.黄芪多糖对蛋鸡生产性能、抗氧化酶活性、免疫机能及肠道主要菌群的影响[D].硕士学位论文.保定:河北农业大学,2010.
[12]何欣,张琳,朱晓宇,等.苍术、黄柏、藿香对夏季蛋用种鸡生产性能的影响[J].北京农学院学报,2006,21(3):66-68.
[13]FANG Y Z,YANG S,WU G Y.Free radicals,antioxidants,and nutrition[J].Nutrition,2002,18(10):872-879.
[14]NG T B,LIU F,WANG Z T.Antioxidative activity of natural products from plants[J].Life Science,2000,66(8):7-23.
[15]郭少英,程发峰,钟相根,等.黄芩苷的体外抗氧化研究[J].时珍国医国药,2011,22(1):9-11.
[16]丁利君,吴倩萍.黄芪多糖的提取及其对自由基的清除作用[J].食品与机械,2003(4):7-9.
[17]ZANG L Y,COSMA G,GARDNER H,et al.Effect of chlorogenic acid on hydroxyl radical[J].M olecular and Cellular Biochemistry,2003,247(1/2):205-210.
[18]陈绍华,王亚琴,罗立新.天然产物绿原酸的研究进展[J].食品科技,2008,33(2):195-199.
[19]YU D H,BAO Y M,WEI C L,et al.Studies of chemical constituents and their antioxidant activities from Astragalus mongholicus Bunge[J].Biomedical and Environmental Sciences,2005,18(5):297-301.
[20]霍书英,李呈敏,王淑荣,等.纯中药饲料添加剂对肉仔鸡增重血清激素水平以及尿酸尿素氮的影响[J].中国兽医杂志,2001,37(10):26-28.
[21]陈鲜鑫,王金全,鲜凌瑾.天然植物提取物对蛋鸡产蛋性能、蛋品质和血清生化免疫指标的影响[J].中国家禽,2017,39(5):35-38.
[22]席利莎,木泰华,孙红男.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301.
[23]许合金,张军民,王修启,等.类黄酮对蛋鸡生产性能、蛋品质和血液生化特性的影响[J].中国畜牧兽医,2009,36(6):19-23.
[2]孙先枝,程建波,卜登攀,等.黄芩苷的生物学功能和黄芩及其提取物在畜禽生产中的应用研究进展[J].动物营养学报,2013,25(7):1459-1464.
[3]LI S P,ZHAO X J,WANG J Y.Synergy of Astragalus polysaccharides and probiotics(Lactobacillus and Bacillus cereus)on immunity and intestinal microbiota in chicks[J].Poultry Science,2009,88(3):519-525.
[4]官丽辉,张立永,刘海斌,等.黄芪多糖对蛋鸡生产性能、生殖激素及血液生理生化指标的影响[J].中国粮油学报,2015,30(7):70-76.
[5]李振.中草药抗热应激剂对蛋鸡生产性能、蛋品质及血液生化指标的影响[J].西北农林科技大学学报(自然科学版),2011,39(9):48-52.
[6]CHEN H L,LI D F,CHANG B Y,et al.Effects of Chinese herbal polysaccharides on the immunity and grow th performance of young broilers[J].Poultry Science,2003,82(3):364-370.
[7]WANG H F,YANG W R,YANG H W,et al.Effects of Astragalus membranaceus on grow th performance,carcass characteristics,and antioxidant status of broiler chickens[J].Acta Agriculturae Scandinavica,Section A:Animal Science,2010,60(3):151-158.
[8]王宇,周选武,陈代文,等.绿原酸对仔猪生长性能、血清免疫球蛋白及肠道黏膜形态与消化吸收能力的影响[J].动物营养学报,2018,30(3):1136-1145.
[9]刘青翠,彭翔,张俊平,等.杜仲叶提取物对产蛋后期蛋鸡生产性能、蛋品质、蛋黄胆固醇含量及血清抗氧化指标的影响[J].动物营养学报,2018,30(1):284-292.
[10]WANG Y Z,YANG W R,WANG Y,et alEffects of Astragalus membranaceus on laying performance and antioxidant status of laying hens[J].The Journal of Applied Poultry Research,2012,21(2):243-250.
[11]王洪芳.黄芪多糖对蛋鸡生产性能、抗氧化酶活性、免疫机能及肠道主要菌群的影响[D].硕士学位论文.保定:河北农业大学,2010.
[12]何欣,张琳,朱晓宇,等.苍术、黄柏、藿香对夏季蛋用种鸡生产性能的影响[J].北京农学院学报,2006,21(3):66-68.
[13]FANG Y Z,YANG S,WU G Y.Free radicals,antioxidants,and nutrition[J].Nutrition,2002,18(10):872-879.
[14]NG T B,LIU F,WANG Z T.Antioxidative activity of natural products from plants[J].Life Science,2000,66(8):7-23.
[15]郭少英,程发峰,钟相根,等.黄芩苷的体外抗氧化研究[J].时珍国医国药,2011,22(1):9-11.
[16]丁利君,吴倩萍.黄芪多糖的提取及其对自由基的清除作用[J].食品与机械,2003(4):7-9.
[17]ZANG L Y,COSMA G,GARDNER H,et al.Effect of chlorogenic acid on hydroxyl radical[J].M olecular and Cellular Biochemistry,2003,247(1/2):205-210.
[18]陈绍华,王亚琴,罗立新.天然产物绿原酸的研究进展[J].食品科技,2008,33(2):195-199.
[19]YU D H,BAO Y M,WEI C L,et al.Studies of chemical constituents and their antioxidant activities from Astragalus mongholicus Bunge[J].Biomedical and Environmental Sciences,2005,18(5):297-301.
[20]霍书英,李呈敏,王淑荣,等.纯中药饲料添加剂对肉仔鸡增重血清激素水平以及尿酸尿素氮的影响[J].中国兽医杂志,2001,37(10):26-28.
[21]陈鲜鑫,王金全,鲜凌瑾.天然植物提取物对蛋鸡产蛋性能、蛋品质和血清生化免疫指标的影响[J].中国家禽,2017,39(5):35-38.
[22]席利莎,木泰华,孙红男.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301.
[23]许合金,张军民,王修启,等.类黄酮对蛋鸡生产性能、蛋品质和血液生化特性的影响[J].中国畜牧兽医,2009,36(6):19-23.