四种检验水中粪大肠菌群标准方法的对比研究
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摘要
多管发酵法、滤膜法、纸片快速法和酶底物法是目前国内测定水中粪大肠菌群的常用标准方法。分别从方法的检出限、方法操作、准确度和方法间的一致性四个方面对水中粪大肠菌群的测定进行了对比研究,总结出各方法的适用范围和优缺点,为分析人员根据实际条件和水样类型选择最佳方法提供参考。
Manifold zymptechnics,filter membrane,paper strip method and enzyme substrate method are the commonly used standard methods for determining fecal coliform in China. From four aspects,the detection limit of the method,the method operation,the accuracy and the consistency amongst the methods were compared,and the advantages and disadvantages and the applicable scope of the methods were summarized,It provides a reference for the lab staff to select the best method according to the actual conditions and the types of water sample.
Manifold zymptechnics,filter membrane,paper strip method and enzyme substrate method are the commonly used standard methods for determining fecal coliform in China. From four aspects,the detection limit of the method,the method operation,the accuracy and the consistency amongst the methods were compared,and the advantages and disadvantages and the applicable scope of the methods were summarized,It provides a reference for the lab staff to select the best method according to the actual conditions and the types of water sample.
引文
[1]国家环境保护总局. HJ/T 347-2007水质粪大肠菌群的测定多管发酵法和滤膜法(试行)[S].北京:中国环境科学出版社,2007.
[2]环境保护部. HJ 755-2015水质总大肠菌群和粪大肠菌群的测定纸片快速法[S].北京:中国环境科学出版社,2015.
[3]陕西省质量技术监督局. DB61/T 1138-2018水质粪大肠菌群的测定酶底物法[S]. 2018.
[4]Clesceri L S. Standard methods for the examination of water and wastewater(20th ed.)[M]. Washington D C:American Public Health Association-American Water Works AssociationWater Environment Federation,1998:954-955,963-965.
[5] B S. ISO 9308-1:2014 Water quality-enumeration of escherichia coli and coliform bacteria-part 1:membrane filtration method for waters with low bacterial background flora[S]. 2014.
[6] B S. ISO 9308-2:2012 Water quality-enumeration of escherichia coli and coliform bacteria-part 2:most probable number method[S]. 2012.
[7]中华人民共和国卫生部,中国国家标准化管理委员会.GB5750. 12-2006生活饮用水标准检验方法微生物指标[S].北京:中国标准出版社,2006.
[8] Hurley M A,Roscoe M E. Automated statistical analysis of microbial enumeration by dilutionseries[J]. The Journal of Applied Bacteriology,2010,55(1):159-164.
[9]汤琳.粪大肠菌群酶底物法在环境监测中的应用研究[J].环境监控与预警,2014,6(3):32-35.
[10]高瑞坤,汤琳.水中粪大肠菌群快速检测方法-固定底物酶底物法的比较[J].中国环境监测,2008,6(4):39-41.
[2]环境保护部. HJ 755-2015水质总大肠菌群和粪大肠菌群的测定纸片快速法[S].北京:中国环境科学出版社,2015.
[3]陕西省质量技术监督局. DB61/T 1138-2018水质粪大肠菌群的测定酶底物法[S]. 2018.
[4]Clesceri L S. Standard methods for the examination of water and wastewater(20th ed.)[M]. Washington D C:American Public Health Association-American Water Works AssociationWater Environment Federation,1998:954-955,963-965.
[5] B S. ISO 9308-1:2014 Water quality-enumeration of escherichia coli and coliform bacteria-part 1:membrane filtration method for waters with low bacterial background flora[S]. 2014.
[6] B S. ISO 9308-2:2012 Water quality-enumeration of escherichia coli and coliform bacteria-part 2:most probable number method[S]. 2012.
[7]中华人民共和国卫生部,中国国家标准化管理委员会.GB5750. 12-2006生活饮用水标准检验方法微生物指标[S].北京:中国标准出版社,2006.
[8] Hurley M A,Roscoe M E. Automated statistical analysis of microbial enumeration by dilutionseries[J]. The Journal of Applied Bacteriology,2010,55(1):159-164.
[9]汤琳.粪大肠菌群酶底物法在环境监测中的应用研究[J].环境监控与预警,2014,6(3):32-35.
[10]高瑞坤,汤琳.水中粪大肠菌群快速检测方法-固定底物酶底物法的比较[J].中国环境监测,2008,6(4):39-41.