北疆地区哈萨克族人群A_2亚型、MNS、P、Kell血型表型分布调查
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摘要
目的了解新疆北疆地区哈萨克族人群A_2亚型、MNS、P、Kell血型表型的多态性,建立哈萨克族人群稀有血型抗原的资料。方法随机选择新疆北疆地区哈萨克族人群的无关个体6 862人,采用血型血清学盐水试管法进行A、B、A_1、M、N、P_1、K抗原检测,采用卡式微柱凝胶抗球蛋白法,进行S、s、k抗原检测。结果 1)受检人群A_2亚型在A型中的检出率为7.08%,AB型中的检出率为21.79%;2)MNS、P、Kell血型系统等位基因频率,依次为:M=0.566 8,N=0.433 2,S=0.186 0,s=0.814 0,P1=0.284 8,P2=0.715 2,K1=0.009 6,K2=0.990 4,用χ~2检验,比较基因型频率分布的观察值与期望值,符合Hardy-Weinberg遗传法则(P>0.05);3)检出MNS血型系统罕见S-s-表现型14例,频率为1.16%。4)Kell血型系统中K抗原频率为1.92%,检出罕见KK纯合子1例,频率为0.034%。结论新疆北疆地区哈萨克族人群A_2亚型、MNS、P、Kell血型基因频率分布,具有本民族特征。MNS血型存在独特的基因类型,Kell血型K抗原阳性率明显高于汉族。
Objective To understand the polymorphisms of the A_2 subtype, MNS, P, and Kell phenotypes in the Kazak population of northern Xinjiang and to establish data on rare blood group antigens in the Kazakh population.Methods 6 862 unrelated individuals in the Kazak population of northern Xinjiang were randomly selected. The antigen of A, B, A_1, M, N, P_1 and K was detected by blood type serological saline test tube method. The antigen of S, s and K was detected by the card type microcolumn gel antiglobulin method. Results 1) A_2 subtype, the detection rate in type A was 7.08%, and the detection rate in AB type was 21.79%. 2) The allele frequencies of MNS, P and Kell blood type were successively:M=0.566 8, N=0.433 2, S=0.186 0, S=0.814 0, P_1=0.284 8, P_2=0.715 2, K1=0.009 6, K2=0.990 4. using the χ~2 test, The Observation value and expectation of genotype frequency distribution were compared. Conform to the Hardy-Weinberg genetic rule(P>0.05). 3) Detecting the rare gene phenotype of MNS blood group system in Kazak population, namely S-s-14 cases, with a frequency of 1.16%. 4) The frequency of K antigen in the Kell blood group system was 1.92%, and 1 cases of rare KK homozygote were detected, with a frequency of 0.034%. Conclusion The gene frequency distribution of A_2 subtype, MNS, P and Kell blood type of the Kazakh population in North Xinjiang in Xinjiang region have the characteristics of own ethnic. The MNSs blood type has a unique genetype. and Kell blood type K antigen positive rate is significantly higher than that of the Han nationality.
Objective To understand the polymorphisms of the A_2 subtype, MNS, P, and Kell phenotypes in the Kazak population of northern Xinjiang and to establish data on rare blood group antigens in the Kazakh population.Methods 6 862 unrelated individuals in the Kazak population of northern Xinjiang were randomly selected. The antigen of A, B, A_1, M, N, P_1 and K was detected by blood type serological saline test tube method. The antigen of S, s and K was detected by the card type microcolumn gel antiglobulin method. Results 1) A_2 subtype, the detection rate in type A was 7.08%, and the detection rate in AB type was 21.79%. 2) The allele frequencies of MNS, P and Kell blood type were successively:M=0.566 8, N=0.433 2, S=0.186 0, S=0.814 0, P_1=0.284 8, P_2=0.715 2, K1=0.009 6, K2=0.990 4. using the χ~2 test, The Observation value and expectation of genotype frequency distribution were compared. Conform to the Hardy-Weinberg genetic rule(P>0.05). 3) Detecting the rare gene phenotype of MNS blood group system in Kazak population, namely S-s-14 cases, with a frequency of 1.16%. 4) The frequency of K antigen in the Kell blood group system was 1.92%, and 1 cases of rare KK homozygote were detected, with a frequency of 0.034%. Conclusion The gene frequency distribution of A_2 subtype, MNS, P and Kell blood type of the Kazakh population in North Xinjiang in Xinjiang region have the characteristics of own ethnic. The MNSs blood type has a unique genetype. and Kell blood type K antigen positive rate is significantly higher than that of the Han nationality.
引文
[1] 肖星甫.输血技术手册.成都:四川科学技术出版社, 1992: 68-70.
[2] 赵桐茂.血型基因频率的估计// ABO血型//赵桐茂.人类血型遗传学.北京:科学技术出版社, 1987: 33,53,229-230, 234,351-352, 364-367.
[3] 血型调查组,我国十六个民族的血型调查报告Ⅰ.ABO血型及ABH物质分泌能力的调查.中华血液学杂志,1980,1(5) :261-263.
[4] Ying Y,Hong X,Xu X,et al. Serological characteristic and mo-lecular basis of A2 subgroup in the Chinese population. Transfus Apher Sci,2013,48(1): 67-74.
[5] 向东,刘曦,郭忠慧,等.上海地区中国人群中ABO亚型的研究.中国输血杂志,2006,19(1): 25-26.
[6] 谢敬文邓诗桢蓝文莉,等. 番禺地区 ABO 亚型基因分型研究.中国输血杂志,2016,29(4): 381-384.
[7] 刘达庄. 主编. 免疫血液学.上海: 上海科学技术出版社, 2002.39.
[8] 查勇,赵茹,黄慧. 芜湖地区汉族献血者 ABO、Rh、MN 和 P 血型的调查. 临床输血与检验,2010,12(3): 266-267.
[9] 罗洪清,元霞,刘凯波,等. 韶关地区人群 ABO、Rh、MN、P 及 Jk(a-b-)血型分布. 中国生物制品学杂志,2008,21(6): 524-526.
[10] 李国良,肖莉,孙瑜,等.江西地区汉族人群ABO、Rh、MN、Kidd、Lewis、P血型系统调查.中国输血杂志,2001,14(增刊):96.
[11] 林国跃,单金晶,张雅楠,等. 中国新疆回族人群9种稀有血型系统基因频率调查研究. 新疆医科大学报,2016,39(8):1026-1031
[12] 洪缨,巩天祥,周昌华,等.成都地区献血人群Kell等9个血型系统抗原基因分型研究.中国输血杂志,2012,25(8):763-765.
[13] 林国跃,杜小璐,单金晶,等. 中国新疆维吾尔族人群 MNS、Duffy 和 Kell 等稀有血型的基因分子遗传分析. 中国组织工程研究,2016,20(1):123-127
[14] 张嵘,田力,李晓娟,等.西藏藏族人群多个红细胞血型系统基因多态性研究.中国输血杂志,2014,27(5): 505-507.
[15] 肖蘅,郝露萍,张明秋,等.基诺族、布朗族和拉祜族的4种红细胞血型分布.人类学学报,2000,19(3): 238-143.
[16] 许煜和,张益莉,艾琼华. 新疆维吾尔族、哈萨克族和蒙古族ABO、MN、P和Rh血型分布.人类学学报, 1993,12(3):290-293.
[17] 杜若甫. 中国人群体遗传学,北京:科学出版杜,2004:108,111-113,129,139.
[18] 彭进,梁延连,徐华,等.西安地区献血人群MNS血型及因多态性研究.中国输血杂志,2017,30(6): 590-592.
[19] Kahar MA,Patel RD Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in blood donors of south Gujarat, India Journal of Transfusion Science,2014,8(1):51-55.
[2] 赵桐茂.血型基因频率的估计// ABO血型//赵桐茂.人类血型遗传学.北京:科学技术出版社, 1987: 33,53,229-230, 234,351-352, 364-367.
[3] 血型调查组,我国十六个民族的血型调查报告Ⅰ.ABO血型及ABH物质分泌能力的调查.中华血液学杂志,1980,1(5) :261-263.
[4] Ying Y,Hong X,Xu X,et al. Serological characteristic and mo-lecular basis of A2 subgroup in the Chinese population. Transfus Apher Sci,2013,48(1): 67-74.
[5] 向东,刘曦,郭忠慧,等.上海地区中国人群中ABO亚型的研究.中国输血杂志,2006,19(1): 25-26.
[6] 谢敬文邓诗桢蓝文莉,等. 番禺地区 ABO 亚型基因分型研究.中国输血杂志,2016,29(4): 381-384.
[7] 刘达庄. 主编. 免疫血液学.上海: 上海科学技术出版社, 2002.39.
[8] 查勇,赵茹,黄慧. 芜湖地区汉族献血者 ABO、Rh、MN 和 P 血型的调查. 临床输血与检验,2010,12(3): 266-267.
[9] 罗洪清,元霞,刘凯波,等. 韶关地区人群 ABO、Rh、MN、P 及 Jk(a-b-)血型分布. 中国生物制品学杂志,2008,21(6): 524-526.
[10] 李国良,肖莉,孙瑜,等.江西地区汉族人群ABO、Rh、MN、Kidd、Lewis、P血型系统调查.中国输血杂志,2001,14(增刊):96.
[11] 林国跃,单金晶,张雅楠,等. 中国新疆回族人群9种稀有血型系统基因频率调查研究. 新疆医科大学报,2016,39(8):1026-1031
[12] 洪缨,巩天祥,周昌华,等.成都地区献血人群Kell等9个血型系统抗原基因分型研究.中国输血杂志,2012,25(8):763-765.
[13] 林国跃,杜小璐,单金晶,等. 中国新疆维吾尔族人群 MNS、Duffy 和 Kell 等稀有血型的基因分子遗传分析. 中国组织工程研究,2016,20(1):123-127
[14] 张嵘,田力,李晓娟,等.西藏藏族人群多个红细胞血型系统基因多态性研究.中国输血杂志,2014,27(5): 505-507.
[15] 肖蘅,郝露萍,张明秋,等.基诺族、布朗族和拉祜族的4种红细胞血型分布.人类学学报,2000,19(3): 238-143.
[16] 许煜和,张益莉,艾琼华. 新疆维吾尔族、哈萨克族和蒙古族ABO、MN、P和Rh血型分布.人类学学报, 1993,12(3):290-293.
[17] 杜若甫. 中国人群体遗传学,北京:科学出版杜,2004:108,111-113,129,139.
[18] 彭进,梁延连,徐华,等.西安地区献血人群MNS血型及因多态性研究.中国输血杂志,2017,30(6): 590-592.
[19] Kahar MA,Patel RD Phenotype frequencies of blood group systems (Rh, Kell, Kidd, Duffy, MNS, P, Lewis, and Lutheran) in blood donors of south Gujarat, India Journal of Transfusion Science,2014,8(1):51-55.