利用体外产气法研究饲粮中性洗涤纤维/淀粉对瘤胃甲烷产量的影响
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摘要
本试验旨在利用体外产气法研究饲粮中性洗涤纤维(NDF)/淀粉对瘤胃甲烷产量的影响,确定饲粮NDF/淀粉的最佳比值。本试验采用单因素试验设计,饲粮NDF/淀粉分别设为0.8、0.9、1.0、1.1、1.2、1.3、1.4、1.5、1.6、1.7、1.8、1.9、2.0、2.2、2.3,共15个处理,每个处理5个重复,分析饲粮NDF/淀粉对瘤胃24和48 h产气量、甲烷浓度、甲烷产量的影响。结果表明:1)饲粮NDF/淀粉为0.8～1.2时,产气量高,甲烷产量低。饲粮NDF/淀粉为1.2时,24和48 h甲烷产量最低。2)各饲粮NDF/淀粉对产气量、甲烷浓度和甲烷产量均存在显著或极显著影响(P<0.05或P<0.01)。3)由线性回归分析可知,饲粮NDF/淀粉对24和48 h的甲烷浓度和甲烷产量呈极显著正相关关系(P<0.01),与24和48 h产气量呈极显著负相关关系(P<0.01)。4)由线性回归分析可知,24 h甲烷产量与乙酸/丙酸呈显著正相关关系(P<0.05)。48 h甲烷产量与24 h甲烷产量、48 h甲烷浓度呈极显著正相关关系(P <0. 01)。饲粮NDF/淀粉与氨态氮(NH3-N)浓度呈极显著负相关关系(P <0.01)。24和48 h甲烷产量、饲粮NDF/淀粉与体外参数具有强相关关系,相关系数分别为0.942、0.982和0.975。综上所述,饲粮NDF/淀粉介于0.8～1.2甲烷减排效果较好。本试验条件下,饲粮NDF/淀粉为1.2对甲烷减排效果最佳。饲粮NDF/淀粉可以作为预测饲粮体外发酵甲烷产量的指标。
This experiment was conducted to study the effects of dietary neutral detergent fiber to starch ratio( NDF/starch) on rumen methane production using in vitro gas production technique. The single factor experiment was screened for the optimal NDF/starch. The NDF/starch in 15 treatments were 0. 8,0. 9,1. 0,1. 1,1.2,1.3,1.4,1.5,1.6,1.7,1.8,1.9,2.0,2.2 and 2.3,with 5 replicates in each treatment,respectively.In vitro gas production method was performed to analyze gas production,methane concentration and methane production at 24 and 48 h. The results showed as follows: 1) the gas production of dietary NDF/starch between 0.8 and 1.2 was higher,methane production was lower. The methane yield of dietary NDF/starch of 1.2 was the lowest. 2) Dietary NDF and starch at different ratios had significant effects on gas production,methane concentration and methane production( P<0.05 or P<0.01). 3) The linear regression analysis showed that significantly positive relationships were found between dietary NDF/starch and the methane concentration and production at 24 and 48 h( P<0.01),and significantly negative relationships were found between dietary NDF/starch and the gas production at 24 and 48 h( P<0.01). 4) The linear regression analysis showed that significantly positive relationships was found between the methane production at 24 h and acetic acid/propionic acid( P<0.05). It was showed that significantly positive relationships were found between the methane production at 24 and 48 h,the methane concentration at 48 h. Significantly negative relationships were found between dietary NDF/starch and ammonia nitrogen( NH3-N) concentration( P<0.01). The methane production at 24 and48 h,dietary NDF/starch were strongly related to parameters in vitro,regression coefficients were 0. 942,0.982 and 0.975,respectively. It is concluded that dietary NDF/starch between 0.8 and 1.2 can promote methane emission reduction,and the effect is the best when dietary NDF/starch is 1.2 under the present experimental conditions. The high regression coefficients indicate that in vitro methane production can be predicted merely based dietary NDF/starch.[Chinese Journal of Animal Nutrition,2019,31(7):3251-3259]
This experiment was conducted to study the effects of dietary neutral detergent fiber to starch ratio( NDF/starch) on rumen methane production using in vitro gas production technique. The single factor experiment was screened for the optimal NDF/starch. The NDF/starch in 15 treatments were 0. 8,0. 9,1. 0,1. 1,1.2,1.3,1.4,1.5,1.6,1.7,1.8,1.9,2.0,2.2 and 2.3,with 5 replicates in each treatment,respectively.In vitro gas production method was performed to analyze gas production,methane concentration and methane production at 24 and 48 h. The results showed as follows: 1) the gas production of dietary NDF/starch between 0.8 and 1.2 was higher,methane production was lower. The methane yield of dietary NDF/starch of 1.2 was the lowest. 2) Dietary NDF and starch at different ratios had significant effects on gas production,methane concentration and methane production( P<0.05 or P<0.01). 3) The linear regression analysis showed that significantly positive relationships were found between dietary NDF/starch and the methane concentration and production at 24 and 48 h( P<0.01),and significantly negative relationships were found between dietary NDF/starch and the gas production at 24 and 48 h( P<0.01). 4) The linear regression analysis showed that significantly positive relationships was found between the methane production at 24 h and acetic acid/propionic acid( P<0.05). It was showed that significantly positive relationships were found between the methane production at 24 and 48 h,the methane concentration at 48 h. Significantly negative relationships were found between dietary NDF/starch and ammonia nitrogen( NH3-N) concentration( P<0.01). The methane production at 24 and48 h,dietary NDF/starch were strongly related to parameters in vitro,regression coefficients were 0. 942,0.982 and 0.975,respectively. It is concluded that dietary NDF/starch between 0.8 and 1.2 can promote methane emission reduction,and the effect is the best when dietary NDF/starch is 1.2 under the present experimental conditions. The high regression coefficients indicate that in vitro methane production can be predicted merely based dietary NDF/starch.[Chinese Journal of Animal Nutrition,2019,31(7):3251-3259]
引文
[1] MOSS A R,JOUANY J P,NEWBOLD J. Methane production by ruminants:its contribution to global warming[J]. Annales de Zootechnie,2000,49(3):231-253.
[2] RODRIGUES P H M.Control and manipulation of ruminal fermentation[M]//MILLEN D D,DE BENI ARRIGONI M,PACHECO R D L. Rumenology.Cham:Springer,2016:157-187.
[3] JOHNSON K A,JOHNSON D E. Methane emissions from cattle[J]. Journal of Animal Science,1995,73(8):2483-2492.
[4]李宗军.瘤胃丙酸发酵的增强策略及其对碳水化合物代谢的动态影响[D].博士学位论文.杨凌:西北农林科技大学,2018:8-19.
[5]程胜利,肖玉萍,杨保平,等.反刍动物甲烷排放现状及调控技术研究进展[J].中国草食动物科学,2013,33(5):56-59.
[6] AGUERRE MJ,WATTIAUX MA,POWELL J M,et al.Effect of forage-to-concentrate ratio in dairy cowdiets on emission of methane,carbon dioxide,and ammonia,lactation performance,and manure excretion[J].Journal of Dairy Science,2011,94(6):3081-3093.
[7] PHILIPPEAU C,LETTAT A,MARTIN C,et al.Effects of bacterial direct-fed microbials on ruminal characteristics,methane emission,and milk fatty acid composition in cows fed high-or low-starch diets[J].Journal of Dairy Science,2017,100(4):2637-2650.
[8] HATEW B,CONE J W,PELLIKAAN W F,et al.Relationship between in vitro and in vivo methane production measured simultaneously with different dietary starch sources and starch levels in dairy cattle[J].Animal Feed Science and Technology,2015,202:20-31.
[9]郑文思,赵广永,张婷婷,等.应用体外发酵法研究高精料饲粮NSC/NDF与甲烷产量之间的关系[J].动物营养学报,2013,25(10):2315-2324.
[10] GOERING H K,VAN SOEST P J.Forage fiber analysis(apparatus,reagents,procedures and some application)[M]. Washington,D. C.:US Agricultural Research Service,1970:1-20.
[11]王全军,毛胜勇,张红霞,等.半胱胺对山羊瘤胃微生物体外发酵的影响[J].华中农业大学学报,2002(6):535-539.
[12]汤少勋,姜海林,周传社,等.不同牧草品种对体外发酵产气特性的影响[J].草业学报,2005,14(3):72-77.
[13]高巍,王新峰,潘晓亮,等.玉米秸青贮与黄贮及苜蓿干草的体外动态消化研究[J].石河子大学学报(自然科学版),2002,6(3):222-225.
[14] COBLENTZ W K,NELLIS S E,HOFFMAN P C,et al. Unique interrelationships between fiber composition,water-soluble carbohydrates,and in vitro gas production for fall-grown oat forages[J].Journal of Dairy Science,2013,96(11):7195-7209.
[15]朱丹,张佩华,赵勐,等.不同中性洗涤纤维与淀粉比例饲粮对体外瘤胃发酵的影响[J].动物营养学报,2015,27(8):2580-2588.
[16] BEAUCHEMIN K A,KREUZER M,O’MARA F,et al.Nutritional management for enteric methane abatement:a review[J].Australian Journal of Experimental Agriculture,2008,48(2):21-27.
[17] MOSS A R,GIVENS D I,GARNSWORTHY P C.The effect of alkali treatment of cereal straws on digestibility and methane production by sheep[J]. Animal Feed Science and Technology,1994,49(3/4):245-259.
[18]赵一广.肉用绵羊甲烷排放的测定与估测模型的建立[D].硕士学位论文.北京:中国农业科学院,2012.
[19] BOUGOUIN A,FERLAY A,DOREAU M,et al.Effects of carbohydrate type or bicarbonate addition to grass silage-based diets on enteric methane emissions and milk fatty acid composition in dairy cows[J].Journal of Dairy Science,2018,101(7):6085-6097.
[20]丁静美,成述儒,邓凯东,等.不同中性洗涤纤维与非纤维性碳水化合物比值饲粮对肉用绵羊甲烷排放的影响[J].动物营养学报,2017,29(3):806-813.
[21]赵勐.日粮中性洗涤纤维和淀粉比例调控奶牛乳成分合成与瘤胃代谢机制研究[D].博士学位论文.北京:中国农业科学院,2015:1-42.
[22]索效军,张年,杨前平,等.花生藤替代颗粒型全混合日粮中苜蓿对湖北黑头羊生长性能、瘤胃发酵及血清生化指标的影响[J].动物营养学报,2019,31(2):865-873.
[23]付瑶,史仁煌,王雅晶,等.体外法研究不同水平中性洗涤纤维日粮对泌乳高峰期奶牛瘤胃发酵和产气量的影响[J].中国畜牧杂志,2016,52(7):46-50.
[24]周怿,刁其玉.反刍动物瘤胃甲烷气体生成的调控[J].草食家畜,2008(4):21-24.
[25]娜仁花.不同日粮对奶牛瘤胃甲烷及氮排放的影响研究[D].博士学位论文.北京:中国农业科学院,2010:3-6.
[26]丁静美,邓凯东,成述儒,等.反刍动物饲粮纤维组分与甲烷排放的研究进展[J].家畜生态学报,2016,37(11):1-5,10.
[27]冯仰廉,李胜利,赵广永,等.牛甲烷排放量的估测[J].动物营养学报,2012,24(1):1-7.
[28] MILLER T L,WOLIN MJ,ZHAO H X,et al.Characteristics of methanogens isolated from bovine rumen[J].Applied and Environmental Microbiology,1986,51(1):201-202.
[29]韩继福,冯仰廉,张晓明,等.阉牛不同日粮的纤维消化、瘤胃内VFA对甲烷产生量的影响[J].中国兽医学报,1997,17(3):278-280.
[30] WANG Y C,JANSSEN P H,LYNCH T A,et al. A mechanistic model of hydrogen-methanogen dynamics in the rumen[J]. Journal of Theoretical Biology,2016,393:75-81.
[31] MUOZ-TAMAYO R,GIGER-REVERDIN S,SAUVANT D.Mechanistic modelling of in vitro fermentation and methane production by rumen microbiota[J].Animal Feed Science and Technology,2016,220:1-21.
[32] RAMIN M,HUHTANEN P. Development of an in vitro method for determination of methane production kinetics using a fully automated in vitro gas system—A modelling approach[J]. Animal Feed Science and Technology,2012,174(3/4):190-200.
[33] MACOME F M,PELLIKAAN W F,SCHONEWILLE J T,et al. In vitro rumen gas and methane production of grass silages differing in plant maturity and nitrogen fertilisation,compared to in vivo enteric methane production[J]. Animal Feed Science and Technology,2017,230:96-102.
[34] DANIELSSON R,RAMIN M,BERTILSSON J,et al.Evaluation of a gas in vitro system for predicting methane production in vivo[J]. Journal of Dairy Science,2017,100(11):8881-8894.
[35]周艳.肉用绵羊生长期甲烷排放规律研究[D].硕士学位论文.阿拉尔:塔里木大学,2018.
[2] RODRIGUES P H M.Control and manipulation of ruminal fermentation[M]//MILLEN D D,DE BENI ARRIGONI M,PACHECO R D L. Rumenology.Cham:Springer,2016:157-187.
[3] JOHNSON K A,JOHNSON D E. Methane emissions from cattle[J]. Journal of Animal Science,1995,73(8):2483-2492.
[4]李宗军.瘤胃丙酸发酵的增强策略及其对碳水化合物代谢的动态影响[D].博士学位论文.杨凌:西北农林科技大学,2018:8-19.
[5]程胜利,肖玉萍,杨保平,等.反刍动物甲烷排放现状及调控技术研究进展[J].中国草食动物科学,2013,33(5):56-59.
[6] AGUERRE MJ,WATTIAUX MA,POWELL J M,et al.Effect of forage-to-concentrate ratio in dairy cowdiets on emission of methane,carbon dioxide,and ammonia,lactation performance,and manure excretion[J].Journal of Dairy Science,2011,94(6):3081-3093.
[7] PHILIPPEAU C,LETTAT A,MARTIN C,et al.Effects of bacterial direct-fed microbials on ruminal characteristics,methane emission,and milk fatty acid composition in cows fed high-or low-starch diets[J].Journal of Dairy Science,2017,100(4):2637-2650.
[8] HATEW B,CONE J W,PELLIKAAN W F,et al.Relationship between in vitro and in vivo methane production measured simultaneously with different dietary starch sources and starch levels in dairy cattle[J].Animal Feed Science and Technology,2015,202:20-31.
[9]郑文思,赵广永,张婷婷,等.应用体外发酵法研究高精料饲粮NSC/NDF与甲烷产量之间的关系[J].动物营养学报,2013,25(10):2315-2324.
[10] GOERING H K,VAN SOEST P J.Forage fiber analysis(apparatus,reagents,procedures and some application)[M]. Washington,D. C.:US Agricultural Research Service,1970:1-20.
[11]王全军,毛胜勇,张红霞,等.半胱胺对山羊瘤胃微生物体外发酵的影响[J].华中农业大学学报,2002(6):535-539.
[12]汤少勋,姜海林,周传社,等.不同牧草品种对体外发酵产气特性的影响[J].草业学报,2005,14(3):72-77.
[13]高巍,王新峰,潘晓亮,等.玉米秸青贮与黄贮及苜蓿干草的体外动态消化研究[J].石河子大学学报(自然科学版),2002,6(3):222-225.
[14] COBLENTZ W K,NELLIS S E,HOFFMAN P C,et al. Unique interrelationships between fiber composition,water-soluble carbohydrates,and in vitro gas production for fall-grown oat forages[J].Journal of Dairy Science,2013,96(11):7195-7209.
[15]朱丹,张佩华,赵勐,等.不同中性洗涤纤维与淀粉比例饲粮对体外瘤胃发酵的影响[J].动物营养学报,2015,27(8):2580-2588.
[16] BEAUCHEMIN K A,KREUZER M,O’MARA F,et al.Nutritional management for enteric methane abatement:a review[J].Australian Journal of Experimental Agriculture,2008,48(2):21-27.
[17] MOSS A R,GIVENS D I,GARNSWORTHY P C.The effect of alkali treatment of cereal straws on digestibility and methane production by sheep[J]. Animal Feed Science and Technology,1994,49(3/4):245-259.
[18]赵一广.肉用绵羊甲烷排放的测定与估测模型的建立[D].硕士学位论文.北京:中国农业科学院,2012.
[19] BOUGOUIN A,FERLAY A,DOREAU M,et al.Effects of carbohydrate type or bicarbonate addition to grass silage-based diets on enteric methane emissions and milk fatty acid composition in dairy cows[J].Journal of Dairy Science,2018,101(7):6085-6097.
[20]丁静美,成述儒,邓凯东,等.不同中性洗涤纤维与非纤维性碳水化合物比值饲粮对肉用绵羊甲烷排放的影响[J].动物营养学报,2017,29(3):806-813.
[21]赵勐.日粮中性洗涤纤维和淀粉比例调控奶牛乳成分合成与瘤胃代谢机制研究[D].博士学位论文.北京:中国农业科学院,2015:1-42.
[22]索效军,张年,杨前平,等.花生藤替代颗粒型全混合日粮中苜蓿对湖北黑头羊生长性能、瘤胃发酵及血清生化指标的影响[J].动物营养学报,2019,31(2):865-873.
[23]付瑶,史仁煌,王雅晶,等.体外法研究不同水平中性洗涤纤维日粮对泌乳高峰期奶牛瘤胃发酵和产气量的影响[J].中国畜牧杂志,2016,52(7):46-50.
[24]周怿,刁其玉.反刍动物瘤胃甲烷气体生成的调控[J].草食家畜,2008(4):21-24.
[25]娜仁花.不同日粮对奶牛瘤胃甲烷及氮排放的影响研究[D].博士学位论文.北京:中国农业科学院,2010:3-6.
[26]丁静美,邓凯东,成述儒,等.反刍动物饲粮纤维组分与甲烷排放的研究进展[J].家畜生态学报,2016,37(11):1-5,10.
[27]冯仰廉,李胜利,赵广永,等.牛甲烷排放量的估测[J].动物营养学报,2012,24(1):1-7.
[28] MILLER T L,WOLIN MJ,ZHAO H X,et al.Characteristics of methanogens isolated from bovine rumen[J].Applied and Environmental Microbiology,1986,51(1):201-202.
[29]韩继福,冯仰廉,张晓明,等.阉牛不同日粮的纤维消化、瘤胃内VFA对甲烷产生量的影响[J].中国兽医学报,1997,17(3):278-280.
[30] WANG Y C,JANSSEN P H,LYNCH T A,et al. A mechanistic model of hydrogen-methanogen dynamics in the rumen[J]. Journal of Theoretical Biology,2016,393:75-81.
[31] MUOZ-TAMAYO R,GIGER-REVERDIN S,SAUVANT D.Mechanistic modelling of in vitro fermentation and methane production by rumen microbiota[J].Animal Feed Science and Technology,2016,220:1-21.
[32] RAMIN M,HUHTANEN P. Development of an in vitro method for determination of methane production kinetics using a fully automated in vitro gas system—A modelling approach[J]. Animal Feed Science and Technology,2012,174(3/4):190-200.
[33] MACOME F M,PELLIKAAN W F,SCHONEWILLE J T,et al. In vitro rumen gas and methane production of grass silages differing in plant maturity and nitrogen fertilisation,compared to in vivo enteric methane production[J]. Animal Feed Science and Technology,2017,230:96-102.
[34] DANIELSSON R,RAMIN M,BERTILSSON J,et al.Evaluation of a gas in vitro system for predicting methane production in vivo[J]. Journal of Dairy Science,2017,100(11):8881-8894.
[35]周艳.肉用绵羊生长期甲烷排放规律研究[D].硕士学位论文.阿拉尔:塔里木大学,2018.