精胺和亚精胺对烟草叶片气体甲醛吸收及生理特性的影响
|
摘要
为了研究多胺参与植物应答气体甲醛胁迫的生理特性,以模式植物烟草为实验材料分析了在气体甲醛胁迫下分别采用100μmol/L精胺和200μmol/L亚精胺预处理对烟草叶片甲醛吸收效率、叶绿素含量、过氧化生理指标、抗氧化酶系统活性及甲醛代谢的影响。结果表明:(1)精胺和亚精胺预处理可以显著提高烟草对1、3和5 mg/m~3气体甲醛的吸收效率,且精胺的效果好于亚精胺。(2)在气体甲醛胁迫下,精胺和亚精胺预处理延缓了烟草叶片中叶绿素b降解,且对过氧化氢(Hydrogen peroxide,H_2O_2)和丙二醛(Malondialdehyde,MDA)的积累有抑制作用。此外,精胺和亚精胺预处理不同程度提高了气体甲醛胁迫下过氧化物酶(Peroxidase,POD)、过氧化氢酶(Catalase,CAT)、抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)和超氧化物歧化酶(Superoxide dismutase,SOD)的活性。(3)精胺与亚精胺预处理对烟草代谢气体甲醛没有促进作用。
This work is to investigate the role and the mechanism of polyamine during the plants response to gaseous formaldehyde stress.With pretreatment of 100 μmol/L spermine and 200 μmol/L spermidine to model plant tobacco,the formaldehyde absorption of tobacco leaves,chlorophyll content,physiological indexes of peroxidation,formaldehyde metabolism,and the activities of the four main antioxidant enzymes(POD,CAT,APX and SOD)under gaseous formaldehyde stress were determined. The results showed that :1)The absorption efficiencyof tobacco under 1,3 and 5 mg/m~3 gaseous formaldehyde significantly increased by the pretreatment with spermine and spermidine,and theeffect by spermine was better than that by spermidine. 2)Under gaseous formaldehyde stress,the pretreatment of spermine and spermidinedelayed the degradation of chlorophyll b,and the accumulations of H_2O_2 and MDA in tobacco leaves were also inhibited. Besides,the activitiesof POD,CAT,APX and SOD were enhanced at varied degree with the pretreatment of spermine and spermidin under gaseous formaldehydestress. 3)The pretreatment of spermine and spermidine presented no promotion on the metabolism of gaseous formaldehyde in tobacco.
This work is to investigate the role and the mechanism of polyamine during the plants response to gaseous formaldehyde stress.With pretreatment of 100 μmol/L spermine and 200 μmol/L spermidine to model plant tobacco,the formaldehyde absorption of tobacco leaves,chlorophyll content,physiological indexes of peroxidation,formaldehyde metabolism,and the activities of the four main antioxidant enzymes(POD,CAT,APX and SOD)under gaseous formaldehyde stress were determined. The results showed that :1)The absorption efficiencyof tobacco under 1,3 and 5 mg/m~3 gaseous formaldehyde significantly increased by the pretreatment with spermine and spermidine,and theeffect by spermine was better than that by spermidine. 2)Under gaseous formaldehyde stress,the pretreatment of spermine and spermidinedelayed the degradation of chlorophyll b,and the accumulations of H_2O_2 and MDA in tobacco leaves were also inhibited. Besides,the activitiesof POD,CAT,APX and SOD were enhanced at varied degree with the pretreatment of spermine and spermidin under gaseous formaldehydestress. 3)The pretreatment of spermine and spermidine presented no promotion on the metabolism of gaseous formaldehyde in tobacco.
引文
[1]LászlóI,Szokeé,Tyihák E.Relationship between abiotic stressand formaldehyde concentration in tissue culture of Datura innoxia Mill[J].Plant Growth Regulation,1998,25(3):195-199.
[2]Tyihák E,Blunden G,Yang MH,et al.Formaldehyde,as itsdimedone adduct,from Ascophyllum nodosum[J].Journal ofApplied Phycology,1996,8(3):211-215.
[3]安雪,李霞,潘会堂,等.16种室内观赏植物对甲醛净化效果及生理生化变化[J].生态环境学报,2010,19(2):379-384.
[4]Sun H,Zhang W,Tang L,et al.Investigation of the role of the calvincycle and C1 metabolism during HCHO metabolism in gaseousHCHO-treated petunia under light and dark conditions using13C-NMR:formaldehyde metabolism in petunia hybrida[J].Phytochemical Analysis,2015,26(3):226-235.
[5]Shirazi AM,Muir PS.In vitro effects of formaldehyde on Douglas firpollen[J].Plant Cell&Environment,1998,21(21):341-346.
[6]Saito Y,Nishio K,Yoshida Y,et al.Cytotoxic effect of formaldehydewith free radicals via increment of cellular reactive oxygenspecies[J].Toxicology,2005,210(2-3):235-245.
[7]李惠民,张莹,贺军民.UV-B对拟南芥叶片不同来源H2O2的活化和气孔关闭的诱导[J].西北植物学报,2013,33(5):911-921.
[8]Groppa MD,Benavides MP.Polyamines and abiotic stress:recentadvances[J].Amino Acids,2008,34(1):35-45.
[9]Galston AW,Sawhney RK.Polyamines in plant physiology[J].Plant Physiology,1990,94(2):406-410.
[10]Pottosin I,Shabala S.Polyamines control of cation transport acrossplant membranes:implications for ion homeostasis and abioticstress signaling[J].Frontiers in Plant Science,2014,5(3):1-16.
[11]Yi TH,Kao CH.Cadmium-induced oxidative damage in rice leavesis reduced by polyamines[J].Plant&Soil,2007,291(1):27-37.
[12]刘强,王庆成,徐静,等.外源亚精胺和精胺对Na HCO3胁迫下南蛇藤抗氧化系统的影响[J].应用生态学报,2009,20(3):549-554.
[13]Capell T,Phillips RL.Modulation of the polyamine biosyntheticpathway in transgenic rice confers tolerance to drought stress[J].Proceedings of the National Academy of Sciences of the UnitedStates of America,2004,101(26):9909-9914.
[14]Wen XP,Ban Y,Inoue H,et al.Aluminum tolerance in aspermidine synthase-overexpressing transgenic European pear iscorrelated with the enhanced level of spermidine via alleviatingoxidative status[J].Environmental&Experimental Botany,2009,66(3):471-478.
[15]Nian HJ,Meng QC,Cheng Q,et al.The effects of overexpressionof formaldehyde dehydrogenase gene from Brevibacillus brevis onthe physiological characteristics of tobacco under formaldehydestress[J].Russian Journal of Plant Physiology,2013,60(6):764-769.
[16]Nian HJ,Meng Q,Zhang W,et al.Overexpression of theformaldehyde dehydrogenase gene from Brevibacillus brevis toenhance formaldehyde tolerance and detoxification of tobacco[J].Applied Biochemistry&Biotechnology,2013,169(1):170-180.
[17]Zhou S,Xiao S,Xuan X,et al.Simultaneous functions of theinstalled DAS/DAK formaldehyde-assimilation pathway and theoriginal formaldehyde metabolic pathways enhance the abilityof transgenic geranium to purify gaseous formaldehyde pollutedenvironment[J].Plant Physiology&Biochemistry,2015,89:53-63.
[18]舒展,张晓素,陈娟,等.叶绿素含量测定的简化[J].植物生理学报,2010,46(4):399-402.
[19]Schmedes A,H?lmer G.A new thiobarbituric acid(TBA)methodfor determining free malondialdehyde(MDA)and hydroperoxidesselectively as a measure of lipid peroxidation.J Am Oil ChemSoc[J].Journal of Oil&Fat Industries,1989,66(6):813-817.
[20]Gay CA,Gebicki JM.Measurement of protein and lipidhydroperoxides in biological systems by the ferric-xylenol orangemethod[J].Analytical Biochemistry,2003,315(1):29-35.
[21]Nakano Y,Asada K.Hydrogen pceroxide is scavenged byascorbate-specific peroxidase in spinach chloroplasts[J].Plant&Cell Physiology,1981,22(5):867-880.
[22]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶(POD)活性变化[J].大豆科学,2013,32(2):197-201.
[23]王群,刘朝巍,徐文娟.紫外分光光度法测定玉米过氧化氢酶活性新进展[J].中国农学通报,2016,32(15):159-165.
[24]Vuleta A,Jovanovi?SM,Tuci?B.Adaptive flexibility of enzymaticantioxidants SOD,APX and CAT to high light stress:Theclonal perennial monocot Iris pumila as a study case[J].PlantPhysiology&Biochemistry,2016,100:166-173.
[25]Kotzabasis K,Fotinou C,Roubelakis-Angelakis KA,et al.Polyamines in the photosynthetic apparatus[J].PhotosynthesisResearch,1993,38(1):83-88.
[26]Wang SS,Song ZB,Sun Z,et al.Effects of formaldehyde stress onphysiological characteristics and gene expression associated withphotosynthesis in Arabidopsis thaliana[J].Plant MolecularBiology Reporter,2012,30(6):1291-1302.
[27]Ray PD,Bo-Wen H,Yoshiaki T.Reactive oxygen species(ROS)homeostasis and redox regulation in cellular signaling[J].Cellular Signalling,2012,24(5):981-990.
[28]孙慧群,周升恩,吴怀胜,等.气体甲醛胁迫对蚕豆保卫细胞中过氧化氢的积累和气孔导度及开度的影响[J].植物生理学报,2015,51(2):246-252.
[29]徐仰仓,王静,刘华,等.外源精胺对小麦幼苗抗氧化酶活性的促进作用[J].植物生理学报,2001,27(4):349-352.
[30]Kamiab F,Talaie A,Khezri M,et al.Exogenous application of freepolyamines enhance salt tolerance of pistachio(Pistacia vera L.)seedlings[J].Plant Growth Regulation,2014,72(3):257-268.
[31]Velikova V,Yordanov I,Edreva A.Oxidative stress and someantioxidant systems in acid rain-treated bean plants:Protectiverole of exogenous polyamines[J].Plant Science,2000,151(1):59-66.
[2]Tyihák E,Blunden G,Yang MH,et al.Formaldehyde,as itsdimedone adduct,from Ascophyllum nodosum[J].Journal ofApplied Phycology,1996,8(3):211-215.
[3]安雪,李霞,潘会堂,等.16种室内观赏植物对甲醛净化效果及生理生化变化[J].生态环境学报,2010,19(2):379-384.
[4]Sun H,Zhang W,Tang L,et al.Investigation of the role of the calvincycle and C1 metabolism during HCHO metabolism in gaseousHCHO-treated petunia under light and dark conditions using13C-NMR:formaldehyde metabolism in petunia hybrida[J].Phytochemical Analysis,2015,26(3):226-235.
[5]Shirazi AM,Muir PS.In vitro effects of formaldehyde on Douglas firpollen[J].Plant Cell&Environment,1998,21(21):341-346.
[6]Saito Y,Nishio K,Yoshida Y,et al.Cytotoxic effect of formaldehydewith free radicals via increment of cellular reactive oxygenspecies[J].Toxicology,2005,210(2-3):235-245.
[7]李惠民,张莹,贺军民.UV-B对拟南芥叶片不同来源H2O2的活化和气孔关闭的诱导[J].西北植物学报,2013,33(5):911-921.
[8]Groppa MD,Benavides MP.Polyamines and abiotic stress:recentadvances[J].Amino Acids,2008,34(1):35-45.
[9]Galston AW,Sawhney RK.Polyamines in plant physiology[J].Plant Physiology,1990,94(2):406-410.
[10]Pottosin I,Shabala S.Polyamines control of cation transport acrossplant membranes:implications for ion homeostasis and abioticstress signaling[J].Frontiers in Plant Science,2014,5(3):1-16.
[11]Yi TH,Kao CH.Cadmium-induced oxidative damage in rice leavesis reduced by polyamines[J].Plant&Soil,2007,291(1):27-37.
[12]刘强,王庆成,徐静,等.外源亚精胺和精胺对Na HCO3胁迫下南蛇藤抗氧化系统的影响[J].应用生态学报,2009,20(3):549-554.
[13]Capell T,Phillips RL.Modulation of the polyamine biosyntheticpathway in transgenic rice confers tolerance to drought stress[J].Proceedings of the National Academy of Sciences of the UnitedStates of America,2004,101(26):9909-9914.
[14]Wen XP,Ban Y,Inoue H,et al.Aluminum tolerance in aspermidine synthase-overexpressing transgenic European pear iscorrelated with the enhanced level of spermidine via alleviatingoxidative status[J].Environmental&Experimental Botany,2009,66(3):471-478.
[15]Nian HJ,Meng QC,Cheng Q,et al.The effects of overexpressionof formaldehyde dehydrogenase gene from Brevibacillus brevis onthe physiological characteristics of tobacco under formaldehydestress[J].Russian Journal of Plant Physiology,2013,60(6):764-769.
[16]Nian HJ,Meng Q,Zhang W,et al.Overexpression of theformaldehyde dehydrogenase gene from Brevibacillus brevis toenhance formaldehyde tolerance and detoxification of tobacco[J].Applied Biochemistry&Biotechnology,2013,169(1):170-180.
[17]Zhou S,Xiao S,Xuan X,et al.Simultaneous functions of theinstalled DAS/DAK formaldehyde-assimilation pathway and theoriginal formaldehyde metabolic pathways enhance the abilityof transgenic geranium to purify gaseous formaldehyde pollutedenvironment[J].Plant Physiology&Biochemistry,2015,89:53-63.
[18]舒展,张晓素,陈娟,等.叶绿素含量测定的简化[J].植物生理学报,2010,46(4):399-402.
[19]Schmedes A,H?lmer G.A new thiobarbituric acid(TBA)methodfor determining free malondialdehyde(MDA)and hydroperoxidesselectively as a measure of lipid peroxidation.J Am Oil ChemSoc[J].Journal of Oil&Fat Industries,1989,66(6):813-817.
[20]Gay CA,Gebicki JM.Measurement of protein and lipidhydroperoxides in biological systems by the ferric-xylenol orangemethod[J].Analytical Biochemistry,2003,315(1):29-35.
[21]Nakano Y,Asada K.Hydrogen pceroxide is scavenged byascorbate-specific peroxidase in spinach chloroplasts[J].Plant&Cell Physiology,1981,22(5):867-880.
[22]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶(POD)活性变化[J].大豆科学,2013,32(2):197-201.
[23]王群,刘朝巍,徐文娟.紫外分光光度法测定玉米过氧化氢酶活性新进展[J].中国农学通报,2016,32(15):159-165.
[24]Vuleta A,Jovanovi?SM,Tuci?B.Adaptive flexibility of enzymaticantioxidants SOD,APX and CAT to high light stress:Theclonal perennial monocot Iris pumila as a study case[J].PlantPhysiology&Biochemistry,2016,100:166-173.
[25]Kotzabasis K,Fotinou C,Roubelakis-Angelakis KA,et al.Polyamines in the photosynthetic apparatus[J].PhotosynthesisResearch,1993,38(1):83-88.
[26]Wang SS,Song ZB,Sun Z,et al.Effects of formaldehyde stress onphysiological characteristics and gene expression associated withphotosynthesis in Arabidopsis thaliana[J].Plant MolecularBiology Reporter,2012,30(6):1291-1302.
[27]Ray PD,Bo-Wen H,Yoshiaki T.Reactive oxygen species(ROS)homeostasis and redox regulation in cellular signaling[J].Cellular Signalling,2012,24(5):981-990.
[28]孙慧群,周升恩,吴怀胜,等.气体甲醛胁迫对蚕豆保卫细胞中过氧化氢的积累和气孔导度及开度的影响[J].植物生理学报,2015,51(2):246-252.
[29]徐仰仓,王静,刘华,等.外源精胺对小麦幼苗抗氧化酶活性的促进作用[J].植物生理学报,2001,27(4):349-352.
[30]Kamiab F,Talaie A,Khezri M,et al.Exogenous application of freepolyamines enhance salt tolerance of pistachio(Pistacia vera L.)seedlings[J].Plant Growth Regulation,2014,72(3):257-268.
[31]Velikova V,Yordanov I,Edreva A.Oxidative stress and someantioxidant systems in acid rain-treated bean plants:Protectiverole of exogenous polyamines[J].Plant Science,2000,151(1):59-66.