三株生防酵母菌对Botrytis cinerea防治效果研究
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摘要
文章研究三株生防酵母菌Cryptococcus albidus 63(Ca63)、Cryptococcus albidus 64(Ca64)和Candida parapsilosis yett1006对番茄灰霉病病原菌B.cinerea t08016b防治效果。番茄果实贮藏试验表明,三株生防酵母菌均有贮藏防腐效果,其中C.albidus 64防腐效果最好,好果率达45.83%。生防酵母菌对番茄灰霉病离体果实防治效果试验表明,三株生防酵母菌均可抑制番茄灰霉病,其中C.albidus 64防治效果最好,单一菌液处理对番茄灰霉病菌防治效果达95.83%,同时接种生防酵母菌和灰霉病菌对番茄灰霉病菌防治效果达69.57%。三株生防酵母菌对番茄灰霉病诱导抗性试验结果表明,三株生防酵母菌均可诱导番茄果实中防御相关酶如多酚氧化酶(PPO)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性且活性氧(H2O2、O2-)含量产生明显提高,可增强番茄对灰霉病抗病能力。
In this study, the control effects of three biocontrol yeasts Cryptococcus albidus 63(Ca63),Cryptococcus albidus 64(Ca64) and Candida parapsilosis yett1006 against Botrytis cinerea were investigated. The storage test of tomato fruit showed that three biocontrol yeasts had anti-corrosion effect.C.albidus 64 had the best anti-corrosion effect and the good fruit rate was 45.83%. The control effect of biocontrol yeasts on isolated fruit of Botrytis cinerea was tested and it was shown that the three biocontrol yeasts could inhibit Botrytis cinerea. C. albidus 64 had the best control effect, with 95.83% of the control effect against Botrytis cinerea treated with a single bacterial solution, and 69.57% of the control effect against Botrytis cinerea inoculated with biocontrol yeast and Botrytis cinerea simultaneously. The results showed that the three biocontrol yeasts could induce the increase of defense-related enzyme activities, such as PPO,POD and PAL, significantly increase active oxygen contents, such as H_2O_2 and O_2~-, and enhance the diseaseresistance of tomato against Botrytis cinerea.
In this study, the control effects of three biocontrol yeasts Cryptococcus albidus 63(Ca63),Cryptococcus albidus 64(Ca64) and Candida parapsilosis yett1006 against Botrytis cinerea were investigated. The storage test of tomato fruit showed that three biocontrol yeasts had anti-corrosion effect.C.albidus 64 had the best anti-corrosion effect and the good fruit rate was 45.83%. The control effect of biocontrol yeasts on isolated fruit of Botrytis cinerea was tested and it was shown that the three biocontrol yeasts could inhibit Botrytis cinerea. C. albidus 64 had the best control effect, with 95.83% of the control effect against Botrytis cinerea treated with a single bacterial solution, and 69.57% of the control effect against Botrytis cinerea inoculated with biocontrol yeast and Botrytis cinerea simultaneously. The results showed that the three biocontrol yeasts could induce the increase of defense-related enzyme activities, such as PPO,POD and PAL, significantly increase active oxygen contents, such as H_2O_2 and O_2~-, and enhance the diseaseresistance of tomato against Botrytis cinerea.
引文
[1]程智慧.蔬菜栽培学各论[M].北京:科学出版社,2010.
[2]赵杨,苗则彦,李颖.番茄灰霉病防治研究进展[J].中国植保导刊,2014,34(7):21-29.
[3]刘宇.利迪链霉素A02对番茄灰霉病的防效及作用机制研究[D].沈阳:沈阳农业大学,2007.
[4]田世平,范青.控制果蔬采后病害的生物学技术[J].植物学通报,2000,17(3):211-217.
[5]Qin G Z,Tian S P,Liu H B,et al.Biocontrol efficacy of three antagonistic yeasts against Penicillium expansum in harvested apple fruits[J].Acta Botanica Sinica,2003,45(4):417-421.
[6]El Ghaouth A,Wilson C L,Wisniewski M.Control of postharvest decay of apple fruit with Candida saitoana and induction of defense responses[J].Phytopathology,2003,93(3):344-348.
[7]靳莎莎,庞水秀,赵利娜等.一株拮抗酵母的筛选鉴定及其对草莓采后病害的生防效果[J].食品科学,2013,34(13):202-208.
[8]龙超安.酵母菌的分离、筛选以及对柑橘保鲜的机理研究[D].武汉:华中农业大学,2005.
[9]王晓东.防治哈密瓜细菌性果斑病拮抗酵母菌的筛选及其生防机理研究[D].武汉:华中农业大学,2009:26-35.
[10]马桂珍.粘帚霉生防菌株发酵液对大豆幼苗中几种防御酶活性的影响[J].吉林农业大学学报.2007,29(3):267-270.
[11]朱广廉.植物生理学试验指导[M].北京:北京大学出版社,1990:37-39.
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[14]关鑫,王傲雪.3株生防酵母菌对Botrytis cinerea的抑菌作用研究[J].东北农业大学学报,2014,45(7):54-60.
[15]张淑珍,靳立梅,徐鹏飞,等.野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶(PAL)活性的变化[J].大豆科学,2009,28(6):1045-1048.
[16]秦国政,田世平,徐勇,等.拮抗菌与病原菌处理对采后桃果实多酚氧化酶、过氧化物酶及苯丙氨酸解氨酶的诱导[J].中国农业科学,2003,36:89-93.
[17]黄丽蝉,欧阳涟,刘文群,等.拮抗菌对柑橘霉变及其抗性的影响[J].食品科学,2008,29(11):643.
[18]高比达,陈捷.生理植物病理学[M].北京:科学出版社,2006.
[19]李波,王军,孙思.植物诱导抗病机制的研究进展[J].中国植保导刊,2013,33(9):19-24.
[20]李征,刘登义,王育鹏,等.活性氧在植物—病原物相互作用过程中的作用[J].安徽师范大学学报:自然科学版,2006,29(1):70-74.
[21]阎士成,李德全,张建华.植物叶片衰老与氧化胁迫[J].植物学通报,1999,16(4):398-404.
[22]董海丽,井金学.活性氧和一氧化氮在植物抗病反应中的作用[J].西北农林科技大学学报:自然科学版,2003,31(1):161-166.
[23]傅爱根,罗广华.活性氧在植物抗病反应中的作用[J].热带亚热带植物学报,2000,8(1):63-69.
[2]赵杨,苗则彦,李颖.番茄灰霉病防治研究进展[J].中国植保导刊,2014,34(7):21-29.
[3]刘宇.利迪链霉素A02对番茄灰霉病的防效及作用机制研究[D].沈阳:沈阳农业大学,2007.
[4]田世平,范青.控制果蔬采后病害的生物学技术[J].植物学通报,2000,17(3):211-217.
[5]Qin G Z,Tian S P,Liu H B,et al.Biocontrol efficacy of three antagonistic yeasts against Penicillium expansum in harvested apple fruits[J].Acta Botanica Sinica,2003,45(4):417-421.
[6]El Ghaouth A,Wilson C L,Wisniewski M.Control of postharvest decay of apple fruit with Candida saitoana and induction of defense responses[J].Phytopathology,2003,93(3):344-348.
[7]靳莎莎,庞水秀,赵利娜等.一株拮抗酵母的筛选鉴定及其对草莓采后病害的生防效果[J].食品科学,2013,34(13):202-208.
[8]龙超安.酵母菌的分离、筛选以及对柑橘保鲜的机理研究[D].武汉:华中农业大学,2005.
[9]王晓东.防治哈密瓜细菌性果斑病拮抗酵母菌的筛选及其生防机理研究[D].武汉:华中农业大学,2009:26-35.
[10]马桂珍.粘帚霉生防菌株发酵液对大豆幼苗中几种防御酶活性的影响[J].吉林农业大学学报.2007,29(3):267-270.
[11]朱广廉.植物生理学试验指导[M].北京:北京大学出版社,1990:37-39.
[12]苍晶,赵会杰.植物生理学实验教程[M].北京:高等教育出版社,2013:150-151.
[13]王爱国.植物的超氧物自由基与羟胺反应的定量关系[J].植物生理学通报,1990(6):55-57.
[14]关鑫,王傲雪.3株生防酵母菌对Botrytis cinerea的抑菌作用研究[J].东北农业大学学报,2014,45(7):54-60.
[15]张淑珍,靳立梅,徐鹏飞,等.野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶(PAL)活性的变化[J].大豆科学,2009,28(6):1045-1048.
[16]秦国政,田世平,徐勇,等.拮抗菌与病原菌处理对采后桃果实多酚氧化酶、过氧化物酶及苯丙氨酸解氨酶的诱导[J].中国农业科学,2003,36:89-93.
[17]黄丽蝉,欧阳涟,刘文群,等.拮抗菌对柑橘霉变及其抗性的影响[J].食品科学,2008,29(11):643.
[18]高比达,陈捷.生理植物病理学[M].北京:科学出版社,2006.
[19]李波,王军,孙思.植物诱导抗病机制的研究进展[J].中国植保导刊,2013,33(9):19-24.
[20]李征,刘登义,王育鹏,等.活性氧在植物—病原物相互作用过程中的作用[J].安徽师范大学学报:自然科学版,2006,29(1):70-74.
[21]阎士成,李德全,张建华.植物叶片衰老与氧化胁迫[J].植物学通报,1999,16(4):398-404.
[22]董海丽,井金学.活性氧和一氧化氮在植物抗病反应中的作用[J].西北农林科技大学学报:自然科学版,2003,31(1):161-166.
[23]傅爱根,罗广华.活性氧在植物抗病反应中的作用[J].热带亚热带植物学报,2000,8(1):63-69.