解淀粉芽孢杆菌LZN01对西瓜专化型尖孢镰刀菌的抑制效应
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摘要
西瓜专化型尖孢镰刀菌(FON)是西瓜枯萎病的病原菌,生物防治是控制西瓜枯萎病的重要手段。研究解淀粉芽孢杆菌LZN01对FON的抑制效应。试验结果表明,解淀粉芽孢杆菌LZN01对FON的抑菌率为57.07%;FON孢子悬液与解淀粉芽孢杆菌LZN01发酵上清液按体积比1∶1混合培养6、12、24 h,孢子萌发抑制率分别为86.21%、67.50%、56.04%,抑制率随时间的增加呈下降趋势;经解淀粉芽孢杆菌LZN01发酵上清液处理,通过扫描电镜观察发现FON菌丝细胞膨大且畸变,菌丝表面严重受损;温度在-20℃～60℃之间,pH值在4～9之间,解淀粉芽孢杆菌LZN01的发酵液抑菌效果稳定;解淀粉芽孢杆菌LZN01发酵上清液抑菌能力稳定。综上所述,解淀粉芽孢杆菌LZN01对FON具有较好的抑制能力,且抑菌效果稳定,在西瓜枯萎病生物防治方面具有较大的开发和应用价值。
引文
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[28]杨丽莉,吕凤霞,别小妹,等.枯草芽孢杆菌抗菌脂肽对嗜水气单胞菌抑菌效果[J].食品科学,2011,32(1):193-198.
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[32]王芳,李静,张欢.青霉菌、放线菌株和石灰水对尖孢镰刀菌抑制作用的研究[J].中国农学通报,2013,29(12):185-189.
[33]张雪花,李琳玲,程华,等.解淀粉芽孢杆菌CMN1308抗真菌特性研究[J].北方园艺,2016(2):117-121.
[2]于天祥,张明方.西瓜枯萎病研究进展[J].中国瓜菜,2004(1):17-19.
[3]任迁琪,吴洪生,李季,等.西瓜枯萎病拮抗链霉菌分离鉴定及盆栽抗病试验[J].生物技术通报,2016,32(9):107-113.
[4]孔庆国,于喜艳.浅谈西瓜育种成就和展望[J].长江蔬菜,2001(8):26-27.
[5]郑琦,毕扬,云晓敏,等.西瓜枯萎病的研究进展及其防治[J].中国植保导刊,2007,27(2):11-13.
[6]丁建成,张其安,方凌,等.西瓜新品种抗枯萎病鉴定[J].中国瓜菜,2005(6):27-29.
[7]王卿.西瓜枯萎病生防细菌的筛选及作用机制初步研究[D].南京:南京师范大学,2013.
[8]王万成.关于西瓜枯萎病及其综合防治的探讨[J].农家之友,2008(12):8-10.
[9]李虎,李广华,戴爱梅.多抗霉素防治西瓜枯萎病药效试验[J].新疆农业科技,2016(1):49-50.
[10]张志明,曾立红,王汉荣.4种药剂防治西瓜枯萎病的效果[J].浙江农业科学,2016,57(12):2063-2064.
[11]严蕾艳,古斌权,马凯慧,等.不同药剂防治西瓜枯萎病田间药效试验[J].长江蔬菜,2016(12):84-86.
[12]Sakata Y,Ohara T,Sugiyama M.The history and present state of the grafting of cucurbitaceous vegetables in Japan[J].Acta Horticulturae,2007,731(731):159-170.
[13]孙义春,苑方武,郭有泉.棚室西瓜枯萎病发生原因与防治[J].北方园艺,2005(5):91.
[14]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[15]Zhang B,Dong C J,Shang Q M,et al.New insights into membrane-active action in plasma membrane of fungal hyphae by the lipopeptide antibiotic bacillomycin L[J].Biochimica et Biophysica Acta-Biomembranes,2013,1828(9):2230-2237.
[16]Rocha F Y O,Oliveira C M D,Silva P R A D,et al.Taxonomical and functional characterization of Bacillus strains isolated from tomato plants and their biocontrol activity against races 1,2 and 3 of Fusarium oxysporum f.sp.lycopersici[J].Applied Soil Ecology,2017,120:8-19.
[17]Wang P P,Guo Q G,Ma Y N,et al.DegQ regulates the production of fengycins and biofilm formation of the biocontrol agent Bacillus subtilis NCD-2[J].Microbiological Research,2015,178:42.
[18]Khochamit N,Siripornadulsil S,Sukon P,et al.Antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing Bacillus subtilis KKU213:potential as a probiotic strain[J].Microbiological Research,2015,170:36.
[19]张艳群,来航线,韦小敏,等.两株生防芽孢细菌筛选、鉴定及拮抗研究[J].微生物学通报,2014,41(2):281-289.
[20]郭志红,李兰珍,梁世平.粘胞白僵菌发育过程的扫描电镜观察[C]//第十次全国电子显微学会议论文集(Ⅰ).1998:379-380.
[21]Nel B,Steinberg C,Labuschagne N,et al.Evaluation of fungicides and sterilants for potential application in the management of Fusarium wilt of banana[J].Crop Protection,2007,26(4):697-705.
[22]游春平,肖爱萍,傅志岸,等.拮抗细菌对香蕉枯萎病的防治效果[J].仲恺农业工程学院学报,2005,18(4):16-20.
[23]刘文波,熊燕红,秦春秀,等.解淀粉芽孢杆菌(Bacillus amyloliquefaciens)HAB-7的培养基优化及抑菌活性[J].中国植保导刊,2017,37(6):5-13.
[24]康金磊,韩旭,张仕琦,等.一株来源于茼蒿根际的西瓜枯萎病拮抗菌的鉴定及特性研究[J].华北农学报,2016,31(增刊1):447-452.
[25]Xu H M,Rong Y J,Zhao M X,et al.Antibacterial activity of the lipopetides produced by Bacillus amyloliquefaciens M1 against multidrug-resistant Vibrio spp.isolated from diseased marine animals[J].Applied Microbiology & Biotechnology,2014,98(1):127.
[26]Cho J,Choi H,Lee J,et al.The antifungal activity and membrane-disruptive action of dioscin extracted from Dioscorea nipponica[J].Biochimica Et Biophysica Acta,2013,1828(3):1153-1158.
[27]Song B,Wang Y Z,Wang G Y,et al.The lipopeptide 6-2 produced by Bacillus amyloliquefaciens anti-CA has potent activity against the biofilm-forming organisms[J].Marine Pollution Bulletin,2016,108(1/2):62.
[28]杨丽莉,吕凤霞,别小妹,等.枯草芽孢杆菌抗菌脂肽对嗜水气单胞菌抑菌效果[J].食品科学,2011,32(1):193-198.
[29]卢春霞.复方植物提取物对嗜水气单胞菌抑菌作用的研究[D].无锡:江南大学,2011.
[30]Lee J,Hwang J S,Hwang B,et al.Influence of the papiliocin peptide derived from Papilio xuthus on the perturbation of fungal cell membranes[J].Fems Microbiology Letters,2010,311(1):70-75.
[31]苏世鸣,任丽轩,杨兴明,等.西瓜专化型尖孢镰刀菌的分离鉴定及水稻根系分泌物对其生长的影响[J].南京农业大学学报,2008,31(1):57-62.
[32]王芳,李静,张欢.青霉菌、放线菌株和石灰水对尖孢镰刀菌抑制作用的研究[J].中国农学通报,2013,29(12):185-189.
[33]张雪花,李琳玲,程华,等.解淀粉芽孢杆菌CMN1308抗真菌特性研究[J].北方园艺,2016(2):117-121.