高压微射流对生物解离大豆膳食纤维特性的影响
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摘要
采用动态高压微射流(Dynamic high pressure microfluidization,DHPM)技术对生物解离大豆膳食纤维进行改性,探讨其对膳食纤维组成、理化及功能特性的影响。结果表明:生物解离和动态高压微射流技术均可有效提高大豆膳食纤维中可溶性膳食纤维的含量,降低不可溶性膳食纤维的含量,使不可溶性膳食纤维与可溶性膳食纤维质量分数的比值达到1.87;通过对比不同处理压力的生物解离大豆膳食纤维中其余成分的含量可知,DHPM对生物解离膳食纤维中含水率影响不显著(P>0.05)。生物解离和动态高压微射流技术能明显改善膳食纤维的水化性质和持油力,但对阳离子交换能力的影响不显著;在pH值7条件下,不同压力处理膳食纤维的重金属离子吸附能力差异不显著,且膳食纤维在pH值7条件下对同种重金属离子的吸附效果优于pH值为2的情况。膳食纤维对葡萄糖吸收能力随处理压力的提高而依次增大,达到200 MPa时略有下降,且不同压力处理膳食纤维的葡萄糖吸收能力均随葡萄糖浓度的增加而提高;200 MPa处理压力下的生物解离膳食纤维的α-淀粉酶抑制能力最高,为18.42%,较0~150 MPa处理的膳食纤维样品分别提高了约36%、32%、28%和27%。随动态高压微射流技术处理压力的增加,膳食纤维结合胆汁酸的能力有所升高。因此,动态高压微射流技术可以作为提高膳食纤维生理功能的有效途径。
The objective was to study the effects of biological dissociation and dynamic high pressure microfluidization(DHPM) on composition, physical and chemical properties and functional characteristics of soybean dietary fiber. The results showed that DHPM produced an increase of soluble dietary fiber(SDF) and a decrease of insoluble dietary fiber(IDF),and raised the IDF/SDF ration to1. 87; in addition, by comparing the contents of the remaining components of the biodissociated soybean dietary fiber with different treatment pressures,it was found that DHPM had no significant effect on the water content of the biodissected dietary fiber(P > 0. 05). DHPM also modified hydration propeities and oil holding capacity greatly,but it did not affect cation exchange capacity significantly;under the condition with pH value of 7,the difference of the adsorption capacity of heavy metal in dietary fiber with different pressures was not significant,and the adsorption effect of dietary fiber on the same heavy metal ion at pH value of 7 was higher than that at pH value of 2. The absorption of glucose by dietary fiber was increased with the increase of treatment pressure,and it was decreased slightly at200 MPa. The glucose absorption capacity of dietary fiber treated with different pressures was increased with the increase of glucose concentration; the highest α-amylase inhibitory capacity was 18. 42 % from dietary fiber,which was increased by approximately 36 %,32 %,28 % and 27 %,respectively,in dietary fiber samples treated from 0 MPa to 150 MPa. The sample showed a higher bile acid bindingcapacity. Therefore,DHPM could be considered as good methods to improve the functionality of dietary fiber in soybean.
The objective was to study the effects of biological dissociation and dynamic high pressure microfluidization(DHPM) on composition, physical and chemical properties and functional characteristics of soybean dietary fiber. The results showed that DHPM produced an increase of soluble dietary fiber(SDF) and a decrease of insoluble dietary fiber(IDF),and raised the IDF/SDF ration to1. 87; in addition, by comparing the contents of the remaining components of the biodissociated soybean dietary fiber with different treatment pressures,it was found that DHPM had no significant effect on the water content of the biodissected dietary fiber(P > 0. 05). DHPM also modified hydration propeities and oil holding capacity greatly,but it did not affect cation exchange capacity significantly;under the condition with pH value of 7,the difference of the adsorption capacity of heavy metal in dietary fiber with different pressures was not significant,and the adsorption effect of dietary fiber on the same heavy metal ion at pH value of 7 was higher than that at pH value of 2. The absorption of glucose by dietary fiber was increased with the increase of treatment pressure,and it was decreased slightly at200 MPa. The glucose absorption capacity of dietary fiber treated with different pressures was increased with the increase of glucose concentration; the highest α-amylase inhibitory capacity was 18. 42 % from dietary fiber,which was increased by approximately 36 %,32 %,28 % and 27 %,respectively,in dietary fiber samples treated from 0 MPa to 150 MPa. The sample showed a higher bile acid bindingcapacity. Therefore,DHPM could be considered as good methods to improve the functionality of dietary fiber in soybean.
引文
1 LIU Chengmei,LIANG Ruihong,DAI Taotao,et al.Effect of dynamic high pressure microfluidization modified insoluble dietary fiber on gelatinization and rheology of rice starch[J].Food Hydrocolloids,2016,57(1):55-61.
2 MYRIAM M L,CATHRINA H E,ALAN R M,et al.Re-evaluation of the mechanisms of dietary fibre and implications for macronutrient bioaccessibility,digestion and postprandial metabolism[J].British Journal of Nutrition,2016,116(5):816-833.
3江连洲,张巧智,李杨,等.大豆水酶法水解液中蛋白质超滤回收及特性研究[J/OL].农业机械学报,2017,48(2):327-334.http:∥www.j-csam.org/jcsam/ch/reader/view_abstract.aspx?flag=1&file_no=20170244&journal_id=jcsam.DOI:10.6041/j.issn.1000-1298.2017.02.044.JIANG Lianzhou,ZHANG Qiaozhi,LI Yang,et al.Ultrafiltration recovery of skim protein from enzyme-assisted aqueous extraction process of soybean and its functional properties[J/OL].Transactions of the Chinese Society for Agricultural Machinery,2017,48(2):327-334.(in Chinese)
4 YE Chen,RAN Ye,LUO Yin,et al.Novel blasting extrusion processing improved the physicochemical properties of soluble dietary fiber from soybean residue and in vivo evaluation[J].Journal of Food Engineering,2014,120(1):1-8.
5李文佳,林亲录,苏小军.从豆渣中制取大豆膳食纤维的研究[J].农产品加工(学刊),2010(6):51-53.LI Wenjia,LIN Qinlu,SU Xiaojun.Study on preparation of soybean dietary fiber from bean dregs[J].Academic Periodical of Farm Products Processing,2010(6):51-53.(in Chinese)
6单平阳,王磊,石可歆,等.花生壳可溶性膳食纤维物化及功能特性的研究[J].安徽农业科学,2017,35(15):89-91,98.SHAN Pingyang,WANG Lei,SHI Kexin,et al.Study on physicochemical and functional properties of soluble dietary fiber from peanut shell[J].Journal of Anhui Agri.Sci.,2017,35(15):89-91,98.(in Chinese)
7冯雁波,包怡红.超微粉碎对松仁膳食纤维体外降血糖、降血脂功能的影响[J].食品工业科技,2016,37(23):342-346,350.FENG Yanbo,BAO Yihong.Effect of ultrafine grinding on hypoglycemic and hypolipidemic functional properties in vitro of dietary fiber from pine residue[J].Science and Technology of Food Industry,2016,37(23):342-346,350.(in Chinese)
8 JIANG Lihua,HUA Di,WANG Zhang,et al.Aqueous enzymatic extraction of peanut oil and protein hydrolysates[J].Food and Bioproducts Processing,2010,88(2-3):233-238.
9 YUSOFF M M,GORDON M H,NIRANJAN K,et al.Aqueous enzyme assisted oil extraction from oilseeds and emulsion deemulsifying methods:a review[J].Trends in Food Science&Technology,2015,41(1):60-82.
10 JASREEN K S,STEPHAINE J,WANG T,et al.Effect of co-products of enzyme-assisted aqueous extraction of soybeans on ethanol production in dry-grind corn fermentation[J].Bioresour.Technol.,2015,192(2):451-460.
11徐渐,江连洲,穆莹.超声波酸水解法提取豆渣中异黄酮条件优化[J].食品工业科技,2012,33(13):253-256.XU Jian,JIANG Lianzhou,MU Ying.Optimization of soybean isoflavone extaraction from okara by ultrasonic and acid hydrolysis[J].Science and Technology of Food Industry,2012,33(13):253-256.(in Chinese)
12 QI Baokun,JIANG Lianzhou,LI Yang,et al.Extract dietary fiber from the soy pods by chemistry-enzymatic methods[J].Procedia Engineering,2011,15(1):4862-4873.
13江连洲,毛惠婷,毕爽,等.超声波辅助提取生物解离豆渣中植酸的工艺优化[J].食品工业科技,2016,37(6):255-259,263.JIANG Lianzhou,MAO Huiting,BI Shuang,et al.Ultrasonic assisted in the extraction of phytic acid from soybean residue produced by enzymatic aqueous processing[J].Science and Technology of Food Industry,2016,37(6):255-259,263.(in Chinese)
14李杨,江连洲,张兆国,等.大豆生物解离提取副产物-水溶性糖工艺优化及机理研究[J].中国粮油学报,2010,25(6):31-36.LI Y,JIANG L Z,ZHANG Z G,et al.Enzyme-assistant aqueous extraction of polysaccharides from soybean by-product:optimization and mechanism study[J].Journal of the Chinese Cereals and Oils Association,2010,25(6):31-36.(in Chinese)
15万婕,余海霞,刘成梅,等.大豆膳食纤维及其各组分连续分离工艺研究[J].食品工业科技,2012,33(24):282-285.WAN Jie,YU Haixia,LIU Chengmei,et al.The continuous separation technology of dietary fiber and its components from soybean[J].Science and Technology of Food Industry,2012,33(24):282-285.(in Chinese)
16赵泰霞,朱杏玲.微生物发酵法提取大豆渣膳食纤维的研究[J].武夷学院学报,2016,35(3):18-22.ZHAO Taixia,ZHU Xingling.A study on the extraction of soybean dietary fiber with fermentation method[J].Journal of Wuyi University,2016,35(3):18-22.(in Chinese)
17涂宗财,胡月明,陈丽莉,等.豆渣膳食纤维吸附重金属的研究[J].食品与机械,2013,29(1):85-87.TU Zongcai,HU Yueming,CHEN Lili,et al.Binding of heavy metals to dietary fibre from soybean residue[J].Food&Machinery,2013,29(1):85-87.(in Chinese)
18 AOAC.Official methods of analysis of the association of official analytical chemists[M].17th Edition.Washington DC,2000.
19 AACC.Approved methods of American Association of Cereal Chemists[M].Appl.Biochem.Biotechnol.,2000.
20 ROBERTSON J A,FDDE M,DYSSELER P,et al.Hydration properties of dietary fibre and resistant starch:an European collaborative study[J].LWT-Food Science and Technology,2000,33(2):72-79.
21阮传英.动态高压微射流技术对豆渣膳食纤维吸附重金属能力的影响[D].南昌:南昌大学,2014.
22 CHAU C F,WEN Y L,WANG Y T.Improvement of the functionality of a potential fruit insoluble fibre by micron technology[J].International Journal of Food Science&Technology,2006,41(9):1054-1060.
23 AHMED F,SAIRAM S,UROOJ A.In vitro hypoglycemic effects of selected dietary fiber sources[J].Journal of Food Science&Technology,2011,48(3):285-289.
24 ADIOTOMRE J,EASTWOOD M A,EDWARDS C A,et al.Dietary fiber:in vitro methods that anticipate nutrition and metabolic activity in humans[J].American Journal of Clinical Nutrition,1990,52(1):128-134.
25马梦梅.孜然膳食纤维改性及降血糖活性研究[D].北京:中国农业科学院,2016.
26 PEREZ-LOPEZE,MATEOS-APARICIO I,RUPEREZ P.Okara treated with high hydrostatic pressure assisted by UltrafloL:effect on solubility of dietary fibre[J].Innovative Food Science&Emerging Technologies,2016,33(2):32-37.
27 GALISTEO M,MORON R,RIVERA L,et al.Plantago ovata husks-supplemented diet ameliorates metabolic alterations in obese Zucker rats through activation of AMP-activated protein kinase.Comparative study with other dietary fibers[J].Clinical Nutrition,2010,29(2):261-267.
28李杨,张雅娜,王欢,等.生物解离提取大豆油与其他不同种大豆油品质差异研究[J].中国粮油学报,2014,29(6):46-52.
29 EVA G,ANTONIO J,PILAR R.Dietary fibre and physicochemical properties of several edible seaweeds from the northwestern Spanish coast[J].Food Research International,2010,43(9):2289-2294.
30 YALEGAMA L L,NEDRA K D,SIVKANESAN R,et al.Chemical and functional properties of fibre concentrates obtained from by-products of coconut kernel[J].Food Chemistry,2013,141(1):124-130.
31 GONI I,MARTINA-CARRON N.In vitro fermentation and hydration properties of commercial dietary fiber-rich supplements[J].Nutrition Research,1998,18(6):1077-1089.
32 ANDERSON E T,BERRY B W.Identification of nonmeat ingredients for increasing fat holding capacity during heating of ground beef[J].Journal of Food Quality,2001,24(4):291-299.
33 BISWAS A K,KUMAR V,BHOSLE S,et al.Dietary fibers as functional ingredients in meat products and their role in human health[J].International Journal of Livestock Production,2011,2(4):45-54.
34 ZHANG Ning,HUANG Caihuan,OU Shiyi.In vitro binding capacities of three dietary fibers and their mixture for four toxic elements,cholesterol,and bile acid[J].Journal of Hazardous Materials,2011,186(1):236-239.
35 PARK K H,LEE K Y,LEE H G.Chemical composition and physicochemical properties of barley dietary fiber by chemical modification[J].International Journal of Biological Macromolecules,2013,60(6):360-365.
36阮传英,涂宗财,王辉,等.豆渣膳食纤维的体外吸附性能[J].食品科学,2014,35(15):109-112.
37刘汉文,黄良策,陈洪兴,等.挤压膨化对豆渣可溶性膳食纤维的影响[J].食品科学,2011,32(8):159-162.
38张华,段倩,张可,等.化学法提取红枣渣中不溶性膳食纤维工艺研究[J].北方园艺,2013,37(14):143-145.
39 LOPEZ-VARGAS J H,FERNANDEZ-LOPEZ J,PEREZ-LVAREZ J A,et al.Chemical,physico-chemical,technological,antibacterial and antioxidant properties of dietary fiber powder obtained from yellow passion fruit(Passiflora edulis var.flavicarpa)co-products[J].Food Research International,2013,51(2):756-763.
2 MYRIAM M L,CATHRINA H E,ALAN R M,et al.Re-evaluation of the mechanisms of dietary fibre and implications for macronutrient bioaccessibility,digestion and postprandial metabolism[J].British Journal of Nutrition,2016,116(5):816-833.
3江连洲,张巧智,李杨,等.大豆水酶法水解液中蛋白质超滤回收及特性研究[J/OL].农业机械学报,2017,48(2):327-334.http:∥www.j-csam.org/jcsam/ch/reader/view_abstract.aspx?flag=1&file_no=20170244&journal_id=jcsam.DOI:10.6041/j.issn.1000-1298.2017.02.044.JIANG Lianzhou,ZHANG Qiaozhi,LI Yang,et al.Ultrafiltration recovery of skim protein from enzyme-assisted aqueous extraction process of soybean and its functional properties[J/OL].Transactions of the Chinese Society for Agricultural Machinery,2017,48(2):327-334.(in Chinese)
4 YE Chen,RAN Ye,LUO Yin,et al.Novel blasting extrusion processing improved the physicochemical properties of soluble dietary fiber from soybean residue and in vivo evaluation[J].Journal of Food Engineering,2014,120(1):1-8.
5李文佳,林亲录,苏小军.从豆渣中制取大豆膳食纤维的研究[J].农产品加工(学刊),2010(6):51-53.LI Wenjia,LIN Qinlu,SU Xiaojun.Study on preparation of soybean dietary fiber from bean dregs[J].Academic Periodical of Farm Products Processing,2010(6):51-53.(in Chinese)
6单平阳,王磊,石可歆,等.花生壳可溶性膳食纤维物化及功能特性的研究[J].安徽农业科学,2017,35(15):89-91,98.SHAN Pingyang,WANG Lei,SHI Kexin,et al.Study on physicochemical and functional properties of soluble dietary fiber from peanut shell[J].Journal of Anhui Agri.Sci.,2017,35(15):89-91,98.(in Chinese)
7冯雁波,包怡红.超微粉碎对松仁膳食纤维体外降血糖、降血脂功能的影响[J].食品工业科技,2016,37(23):342-346,350.FENG Yanbo,BAO Yihong.Effect of ultrafine grinding on hypoglycemic and hypolipidemic functional properties in vitro of dietary fiber from pine residue[J].Science and Technology of Food Industry,2016,37(23):342-346,350.(in Chinese)
8 JIANG Lihua,HUA Di,WANG Zhang,et al.Aqueous enzymatic extraction of peanut oil and protein hydrolysates[J].Food and Bioproducts Processing,2010,88(2-3):233-238.
9 YUSOFF M M,GORDON M H,NIRANJAN K,et al.Aqueous enzyme assisted oil extraction from oilseeds and emulsion deemulsifying methods:a review[J].Trends in Food Science&Technology,2015,41(1):60-82.
10 JASREEN K S,STEPHAINE J,WANG T,et al.Effect of co-products of enzyme-assisted aqueous extraction of soybeans on ethanol production in dry-grind corn fermentation[J].Bioresour.Technol.,2015,192(2):451-460.
11徐渐,江连洲,穆莹.超声波酸水解法提取豆渣中异黄酮条件优化[J].食品工业科技,2012,33(13):253-256.XU Jian,JIANG Lianzhou,MU Ying.Optimization of soybean isoflavone extaraction from okara by ultrasonic and acid hydrolysis[J].Science and Technology of Food Industry,2012,33(13):253-256.(in Chinese)
12 QI Baokun,JIANG Lianzhou,LI Yang,et al.Extract dietary fiber from the soy pods by chemistry-enzymatic methods[J].Procedia Engineering,2011,15(1):4862-4873.
13江连洲,毛惠婷,毕爽,等.超声波辅助提取生物解离豆渣中植酸的工艺优化[J].食品工业科技,2016,37(6):255-259,263.JIANG Lianzhou,MAO Huiting,BI Shuang,et al.Ultrasonic assisted in the extraction of phytic acid from soybean residue produced by enzymatic aqueous processing[J].Science and Technology of Food Industry,2016,37(6):255-259,263.(in Chinese)
14李杨,江连洲,张兆国,等.大豆生物解离提取副产物-水溶性糖工艺优化及机理研究[J].中国粮油学报,2010,25(6):31-36.LI Y,JIANG L Z,ZHANG Z G,et al.Enzyme-assistant aqueous extraction of polysaccharides from soybean by-product:optimization and mechanism study[J].Journal of the Chinese Cereals and Oils Association,2010,25(6):31-36.(in Chinese)
15万婕,余海霞,刘成梅,等.大豆膳食纤维及其各组分连续分离工艺研究[J].食品工业科技,2012,33(24):282-285.WAN Jie,YU Haixia,LIU Chengmei,et al.The continuous separation technology of dietary fiber and its components from soybean[J].Science and Technology of Food Industry,2012,33(24):282-285.(in Chinese)
16赵泰霞,朱杏玲.微生物发酵法提取大豆渣膳食纤维的研究[J].武夷学院学报,2016,35(3):18-22.ZHAO Taixia,ZHU Xingling.A study on the extraction of soybean dietary fiber with fermentation method[J].Journal of Wuyi University,2016,35(3):18-22.(in Chinese)
17涂宗财,胡月明,陈丽莉,等.豆渣膳食纤维吸附重金属的研究[J].食品与机械,2013,29(1):85-87.TU Zongcai,HU Yueming,CHEN Lili,et al.Binding of heavy metals to dietary fibre from soybean residue[J].Food&Machinery,2013,29(1):85-87.(in Chinese)
18 AOAC.Official methods of analysis of the association of official analytical chemists[M].17th Edition.Washington DC,2000.
19 AACC.Approved methods of American Association of Cereal Chemists[M].Appl.Biochem.Biotechnol.,2000.
20 ROBERTSON J A,FDDE M,DYSSELER P,et al.Hydration properties of dietary fibre and resistant starch:an European collaborative study[J].LWT-Food Science and Technology,2000,33(2):72-79.
21阮传英.动态高压微射流技术对豆渣膳食纤维吸附重金属能力的影响[D].南昌:南昌大学,2014.
22 CHAU C F,WEN Y L,WANG Y T.Improvement of the functionality of a potential fruit insoluble fibre by micron technology[J].International Journal of Food Science&Technology,2006,41(9):1054-1060.
23 AHMED F,SAIRAM S,UROOJ A.In vitro hypoglycemic effects of selected dietary fiber sources[J].Journal of Food Science&Technology,2011,48(3):285-289.
24 ADIOTOMRE J,EASTWOOD M A,EDWARDS C A,et al.Dietary fiber:in vitro methods that anticipate nutrition and metabolic activity in humans[J].American Journal of Clinical Nutrition,1990,52(1):128-134.
25马梦梅.孜然膳食纤维改性及降血糖活性研究[D].北京:中国农业科学院,2016.
26 PEREZ-LOPEZE,MATEOS-APARICIO I,RUPEREZ P.Okara treated with high hydrostatic pressure assisted by UltrafloL:effect on solubility of dietary fibre[J].Innovative Food Science&Emerging Technologies,2016,33(2):32-37.
27 GALISTEO M,MORON R,RIVERA L,et al.Plantago ovata husks-supplemented diet ameliorates metabolic alterations in obese Zucker rats through activation of AMP-activated protein kinase.Comparative study with other dietary fibers[J].Clinical Nutrition,2010,29(2):261-267.
28李杨,张雅娜,王欢,等.生物解离提取大豆油与其他不同种大豆油品质差异研究[J].中国粮油学报,2014,29(6):46-52.
29 EVA G,ANTONIO J,PILAR R.Dietary fibre and physicochemical properties of several edible seaweeds from the northwestern Spanish coast[J].Food Research International,2010,43(9):2289-2294.
30 YALEGAMA L L,NEDRA K D,SIVKANESAN R,et al.Chemical and functional properties of fibre concentrates obtained from by-products of coconut kernel[J].Food Chemistry,2013,141(1):124-130.
31 GONI I,MARTINA-CARRON N.In vitro fermentation and hydration properties of commercial dietary fiber-rich supplements[J].Nutrition Research,1998,18(6):1077-1089.
32 ANDERSON E T,BERRY B W.Identification of nonmeat ingredients for increasing fat holding capacity during heating of ground beef[J].Journal of Food Quality,2001,24(4):291-299.
33 BISWAS A K,KUMAR V,BHOSLE S,et al.Dietary fibers as functional ingredients in meat products and their role in human health[J].International Journal of Livestock Production,2011,2(4):45-54.
34 ZHANG Ning,HUANG Caihuan,OU Shiyi.In vitro binding capacities of three dietary fibers and their mixture for four toxic elements,cholesterol,and bile acid[J].Journal of Hazardous Materials,2011,186(1):236-239.
35 PARK K H,LEE K Y,LEE H G.Chemical composition and physicochemical properties of barley dietary fiber by chemical modification[J].International Journal of Biological Macromolecules,2013,60(6):360-365.
36阮传英,涂宗财,王辉,等.豆渣膳食纤维的体外吸附性能[J].食品科学,2014,35(15):109-112.
37刘汉文,黄良策,陈洪兴,等.挤压膨化对豆渣可溶性膳食纤维的影响[J].食品科学,2011,32(8):159-162.
38张华,段倩,张可,等.化学法提取红枣渣中不溶性膳食纤维工艺研究[J].北方园艺,2013,37(14):143-145.
39 LOPEZ-VARGAS J H,FERNANDEZ-LOPEZ J,PEREZ-LVAREZ J A,et al.Chemical,physico-chemical,technological,antibacterial and antioxidant properties of dietary fiber powder obtained from yellow passion fruit(Passiflora edulis var.flavicarpa)co-products[J].Food Research International,2013,51(2):756-763.