基于栅藻延迟发光的制天南星、胆南星药性研究
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摘要
目的寻找表征制天南星、胆南星寒热药性的延迟发光指标。方法非等间隔测量制天南星煎煮后26d内其煎煮液加入栅藻中的延迟发光,验证自然延迟发光平均强度和激发延迟发光线性拟合斜率(k)的稳定性。生物指示剂法测量6个批次制天南星、胆南星的延迟发光,高效液相色谱法测定制天南星、胆南星中β-谷甾醇的含量及胆南星中胆红素和胆酸的含量,并探索k值与成分含量的相关性。结果制天南星煎煮后14 d内k值较自然延迟发光平均强度更稳定。6个批次的制天南星的k值均高于胆南星。6个批次的制天南星、胆南星中β-谷甾醇含量与k值呈负相关,且相关关系显著,胆南星中胆红素和胆酸的含量与胆南星k值呈不显著的负相关。结论 k值是反映制天南星、胆南星药性的科学指标,为研究中药药性提供了新的方向。
Objective To find delayed luminescence parameters that could characterize the cold and hot properties of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile. Methods Delayed luminescence of Arisaematis Rhizoma Preparatum with addition of Scenedesmus sp. within 26 d after decoction was measured in unequal interval, with aim to verify the stability of the natural delayed luminescence average strength and the linear fitting slope value(k) of excitation delayed luminescence. The delayed luminescence of six batches of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile was measured using biological indicator method, and the content of β-sitosterol in Arisaematis Rhizoma Preparatum and β-sitosterol, bilirubin, and cholic acid of Arisaema Cum Bile was determined using high performance liquid chromatograph(HPLC) to analyze the correlation of k value and the above components content of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile. Results K value of excitation delayed luminescence within 14 d after decoction was steadier than natural delayed luminescence average strength, and k values of six batches of Arisaematis Rhizoma Preparatum were all higher than that of Arisaema Cum Bile. A significant negative correlation between β-sitosterol contents and k values of six batches of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile was found, and no significant negative correlation between bilirubin and cholic acid contents and k values of Arisaema Cum Bile was found. Conclusion K value of excitation delayed luminescence could indicate the differences of medicinal properties of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile, which provides a new method for the study of medicinal properties of Chinese materia medica.
Objective To find delayed luminescence parameters that could characterize the cold and hot properties of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile. Methods Delayed luminescence of Arisaematis Rhizoma Preparatum with addition of Scenedesmus sp. within 26 d after decoction was measured in unequal interval, with aim to verify the stability of the natural delayed luminescence average strength and the linear fitting slope value(k) of excitation delayed luminescence. The delayed luminescence of six batches of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile was measured using biological indicator method, and the content of β-sitosterol in Arisaematis Rhizoma Preparatum and β-sitosterol, bilirubin, and cholic acid of Arisaema Cum Bile was determined using high performance liquid chromatograph(HPLC) to analyze the correlation of k value and the above components content of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile. Results K value of excitation delayed luminescence within 14 d after decoction was steadier than natural delayed luminescence average strength, and k values of six batches of Arisaematis Rhizoma Preparatum were all higher than that of Arisaema Cum Bile. A significant negative correlation between β-sitosterol contents and k values of six batches of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile was found, and no significant negative correlation between bilirubin and cholic acid contents and k values of Arisaema Cum Bile was found. Conclusion K value of excitation delayed luminescence could indicate the differences of medicinal properties of Arisaematis Rhizoma Preparatum and Arisaema Cum Bile, which provides a new method for the study of medicinal properties of Chinese materia medica.
引文
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[24]Sun M,Chang W T,Wijk E V,et al.Characterization of the therapeutic properties of Chinese herbal materials by measuring delayed luminescence and dendritic cell-based immunomodulatory response[J].J Photochem Photobiol B,2017,doi:10.1016/j.jphotobiol.2017.01.018.
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[26]朱国念,洪健.斜生栅藻细胞超微结构的TEM观察[J].杭州电子工业学院学报,2000,20(3):75-77.
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[29]付加雷,庞靖祥,聂晓艳,等.栅藻延迟发光的初步研究[J].生物医学工程研究,2015,34(1):49-53.
[30]聂晓艳,付加雷,庞靖祥,等.栅藻生理特性的超微弱发光初探[J].生物医学工程研究,2015,34(4):255-258.
[31]聂晓艳.绿藻门栅藻生物发光的初步研究[D].济南:济南大学,2016.
[32]付加雷.栅藻延迟发光的检测及其在中药药性研究中的应用[D].济南:山东中医药大学,2016.
[33]毕荣璐,孙荣飞,李德勋,等.HPLC法测定不同产地天麻中的β-谷甾醇含量[J].西部林业大学,2014,43(6):116-119.
[34]卢润枚,于沛,高伟贤.HPLC法测定清开灵口服液中胆酸的含量[J].今日药学,2009,19(12):49-51.
[35]顾樵.生物光子学[M].第2版.北京:科学出版社,2012.
[36]李虹,唐雯.中药袋装煎煮液的微生物限度检查[J].药学实践杂志,2007,25(3):156-158.
[37]杨波,王振国.植物类中药寒热药性与化学成分相关性的文献研究[D].济南:山东中医药大学,2010.
[38]刘银梅.炮制对于中药药性改变的研究进展[J].中西医结合心血管病杂志,2016,4(23):136-137.
[39]张培利,郭慧娴,张国丽.炮制对中药四气五味及归经的药性影响分析[J].中国现代药物应用,2014,8(22):192-193.
[40]白宗利,任玉珍,陈彦琳,等.胆南星的研究进展[J].中国现代中药,2010,12(4):15-18.
[41]王薇,王珊,刘超,等.基于小鼠生理生化指标的天南星与胆南星的寒热药性探讨[J].时珍国医国药,2012,23(12):3037-3038.
[42]韩金祥,韩奕.论中医气的物质基础是机体辐射电磁(量子)场[J].山东中医药大学学报,2010,34(6):474-476.
[43]韩金祥.中药药性的科学内涵[J].中华中医药学刊,2011,29(9):1937-1939.
[2]张学华,张群,王蓓.制天南星、胆南星、生天南星临床如何区别应用[J].中医杂志,2008,49(7):663.
[3]吴鲁东,郝虹,李书渊.天南星炮制过程中总生物碱的动态变化[J].海峡药学,2015,27(3):37-39.
[4]季申,丁声颂.10种药用天南星的化学成分分析[J].复旦学报:医学版,1989(3):203-208.
[5]居羚,池玉梅,吴皓,等.超高效液相-高分辨串联质谱分析天南星化学成分[J].亚太传统医药,2016,12(5):45-49.
[6]罗芬,陆丹,池玉梅,等.天南星药材的HPLC指纹图谱研究[J].中国中药杂志,2011,36(23):3302-3305.
[7]徐大林,朴花子,陈正爱.异叶天南星的药理作用研究进展[J].延边大学医学学报,2013,36(4):322-324.
[8]徐皓.天南星的化学成分与药理作用研究进展[J].中国药房,2011,22(11):1046-1048.
[9]陈江宁,单国顺,赵启苗,等.不同胆汁制胆南星中胆酸类成分及其解热作用比较[J].现代药物与临床,2017,32(4):567-571.
[10]满薇.天南星科植物虎掌南星的化学成分及天南星的质量控制研究[D].长春:长春中医药大学,2012.
[11]黎量,李欣逸,艾莉.胆南星炮制及质量标准研究进展与思考[J].中药与临床,2015,6(3):7-9.
[12]汪荣斌,刘晓龙,王存琴,等.天南星的本草考证与药用品种调查[J].中药材,2010,33(7):1182-1185.
[13]唐力英,吴宏伟,王祝举,等.天南星炮制减毒机制探讨(I)[J].中国实验方剂学杂志,2013,19(3):1-4.
[14]杨书彬,王喜军.天南星的毒性认识及毒性研究现状[J].中医药学报,2009,37(5):102-104.
[15]Popp F A,Yan Y.Delayed luminescence of biological systems in terms of coherent states[J].Phys Lett A,2002,293(1):93-97.
[16]Peterson K L,Margherio M J,Doan P,et al.Basis for sensitive and selective time-delayed luminescence detection of hydroxyl radical by lanthanide complexes[J].Inorg Chem,2013,52(16):9390-9398.
[17]Triglia A,Malfa G L,Musumeci F,et al.Delayed luminescence as an indicator of tomato fruit quality[J].JFood Sci,1998,63(3):512-515.
[18]Abbott J A.Quality measurement of fruits and vegetables[J].Postharvest Biol Tec,1999,15(3):207-225.
[19]Vesetova T V,Veselovsky V A,Rubin A B,et al.Delayed luminescence of air-dry soybean seeds as a measure of their viability[J].Physiol Plantarum,2010,65(4):493-497.
[20]Scordino A,Baran I,Gulino M,et al.Ultra-weak delayed luminescence in cancer research:A review of the results by the ARETUSA equipment[J].J Photochem Photobiol B,2014,doi:10.1016/j.jphotobiol.2014.03.027.
[21]崔向阳,刘亚宁,赵新华,等.运用超微弱发光探测技术对中药药性的研究[J].空军总医院学报,1992,8(1):19-20.
[22]Sun M,Wijk R,Wijk E,et al.Delayed luminescence:An experimental protocol for Chinese herbal medicines[J].Luminescence,2016,31(6):1220-1228.
[23]Sun M,Li L,Wang M,et al.Effects of growth altitude on chemical constituents and delayed luminescence properties in medicinal rhubarb[J].J Photochem Photobiol B,2016,162:24-33.
[24]Sun M,Chang W T,Wijk E V,et al.Characterization of the therapeutic properties of Chinese herbal materials by measuring delayed luminescence and dendritic cell-based immunomodulatory response[J].J Photochem Photobiol B,2017,doi:10.1016/j.jphotobiol.2017.01.018.
[25]Pang J,Fu J,Yang M,et al.Correlation between the different therapeutic properties of Chinese medicinal herbs and delayed luminescence[J].Luminescence,2016,31(2):323-327.
[26]朱国念,洪健.斜生栅藻细胞超微结构的TEM观察[J].杭州电子工业学院学报,2000,20(3):75-77.
[27]朱歆莹,高为,钟运建.重金属离子对斜生栅列藻毒性的研究[J].环境监控与预警,2010,2(5):44-45.
[28]贾寒,庞靖祥,杨美娜,等.中药生地黄对栅藻生物光子辐射的影响[J].生物医学工程研究,2016,35(4):298-302.
[29]付加雷,庞靖祥,聂晓艳,等.栅藻延迟发光的初步研究[J].生物医学工程研究,2015,34(1):49-53.
[30]聂晓艳,付加雷,庞靖祥,等.栅藻生理特性的超微弱发光初探[J].生物医学工程研究,2015,34(4):255-258.
[31]聂晓艳.绿藻门栅藻生物发光的初步研究[D].济南:济南大学,2016.
[32]付加雷.栅藻延迟发光的检测及其在中药药性研究中的应用[D].济南:山东中医药大学,2016.
[33]毕荣璐,孙荣飞,李德勋,等.HPLC法测定不同产地天麻中的β-谷甾醇含量[J].西部林业大学,2014,43(6):116-119.
[34]卢润枚,于沛,高伟贤.HPLC法测定清开灵口服液中胆酸的含量[J].今日药学,2009,19(12):49-51.
[35]顾樵.生物光子学[M].第2版.北京:科学出版社,2012.
[36]李虹,唐雯.中药袋装煎煮液的微生物限度检查[J].药学实践杂志,2007,25(3):156-158.
[37]杨波,王振国.植物类中药寒热药性与化学成分相关性的文献研究[D].济南:山东中医药大学,2010.
[38]刘银梅.炮制对于中药药性改变的研究进展[J].中西医结合心血管病杂志,2016,4(23):136-137.
[39]张培利,郭慧娴,张国丽.炮制对中药四气五味及归经的药性影响分析[J].中国现代药物应用,2014,8(22):192-193.
[40]白宗利,任玉珍,陈彦琳,等.胆南星的研究进展[J].中国现代中药,2010,12(4):15-18.
[41]王薇,王珊,刘超,等.基于小鼠生理生化指标的天南星与胆南星的寒热药性探讨[J].时珍国医国药,2012,23(12):3037-3038.
[42]韩金祥,韩奕.论中医气的物质基础是机体辐射电磁(量子)场[J].山东中医药大学学报,2010,34(6):474-476.
[43]韩金祥.中药药性的科学内涵[J].中华中医药学刊,2011,29(9):1937-1939.