生物膜中活性菌株的分离纯化及抑藻活性研究
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摘要
对防污漆表面细菌黏膜进行分离纯化,并对分离纯化的6种活性菌株进行发酵,制得发酵液并离心获得上清液。以中肋骨条藻为受试浮游藻类,采用活性菌株发酵液及上清液对其进行抑藻试验的研究。研究结果表明,细菌发酵液对中肋骨条藻的抑制作用要稍弱于其上清液的抑制作用,6种细菌对中肋骨条藻表现出的抑制作用大小为:Aureobasidium pullulans HN最强;其次为Bacillus cereus和Pseudoalteromonas;Exiguobacterium indicum、Exiguobacterium acetylicum和Kytococcus sedentarius抑制作用相差不大。
The bacterial mucosa on the surface of the antifouling paint was extracted and purified,and the obtained six kinds of active strains were fermented to obtain a fermentation broth. And the supernatant was obtained by centrifugation. Skeletonema costatum was used as the planktonic algae,and the algal growth inhibition test was carried out by using six active strain fermentation broth and supernatant. The research results showed that the inhibitory effect of bacterial fermentation broth on Skeletonema costatum were slightly weaker than that of the supernatant. The inhibitory effects of six kinds of bacteria on Skeletonema costatum were as follows :Aureobasidium pullulans HN was the best,followed by Bacillus cereus and Pseudoalteromonas while the inhibitory effects of Exiguobacterium indicum,Exiguobacterium acetylicum and Kytococcus sedentarius were similar.
The bacterial mucosa on the surface of the antifouling paint was extracted and purified,and the obtained six kinds of active strains were fermented to obtain a fermentation broth. And the supernatant was obtained by centrifugation. Skeletonema costatum was used as the planktonic algae,and the algal growth inhibition test was carried out by using six active strain fermentation broth and supernatant. The research results showed that the inhibitory effect of bacterial fermentation broth on Skeletonema costatum were slightly weaker than that of the supernatant. The inhibitory effects of six kinds of bacteria on Skeletonema costatum were as follows :Aureobasidium pullulans HN was the best,followed by Bacillus cereus and Pseudoalteromonas while the inhibitory effects of Exiguobacterium indicum,Exiguobacterium acetylicum and Kytococcus sedentarius were similar.
引文
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2 郑纪勇.海洋生物污损与材料腐蚀[J].中国腐蚀与防护学报,2010(2):171-176.
3 张璇.船舶仿生防污沟槽表面减阻性能数值模拟分析[D].湖北武汉:武汉理工大学,2014.
4 Michael P Schultz.Frictional Resistance of Antifouling Coating Systems[J].J Fluids Eng.,2005,126(6):1 039-1 047.
5 柏芳,王泽华,王国伟,等.防海生物污损材料研究现状[J].腐蚀与防护,2014(5):420-424.
6 严涛,刘姗姗,曹文浩.中国沿海水产设施污损生物特点及防除途径[J].海洋通报,2008,27(1):102-110.
7 张涛,彭飞,闵少松.海洋污损对螺旋桨叶切面性能影响的数值模拟[J].中国舰船研究,2016,11(3):32-36.
8 梁琳.最具威胁性物种半数进入中国[J].生态经济,2015,31(9):10-13.
9 戴宇均,汪鹏程,孙巨福.船舶防污涂料的研究进展[J].安徽化工,2013,39(5):10-12.
10 张金伟,郑纪勇,王利,等.仿生防污材料的研究进展[J].中国材料进展,2014,33(2):86-94.
11 丛巍巍,肖玲,王科,等.天然防污剂的研究现状[J].材料导报,2012,26(1):334-336.
12 Rijstenbil J W.Phytoplankton Composition of Stagnant and Tidal Ecosystems in Relation to Salinity,Nutrients,Light and Turbulence[J].Netherlands Journal of Sea Research,1987,21(2):113-123.
13 王朝晖,袁美玲,骆育敏,等.海洋卡盾藻与中肋骨条藻和锥状斯氏藻种间竞争研究[J].水生生物学报,2010,34(2):336-34.
14 高敏.海洋微生物和微藻中防污活性物质的提取及其性能研究[D].山东青岛:中国海洋大学,2014.
15 别聪聪,李锋民,李媛媛,等.六种大型藻浸提液对中肋骨条藻的抑制及活性成分分离[J].中国海洋大学学报(自然科学版),2011,41(7/8):107-112.