佩连麻黄方对单纯性肥胖大鼠体质量、肝功能、血脂的影响
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摘要
目的:探讨佩连麻黄方对单纯性肥胖大鼠体质量的影响及其对改善肝功能、降低血脂水平和调节脂代谢紊乱的作用。方法:将大鼠随机平均分为模型组、对照组、佩连麻黄方低剂量组、中剂量组以及高剂量组。制作肥胖大鼠模型,在给予大鼠药物8周后,对大鼠的体长、体质量、Lee′s指数,体脂含量进行检测。对血脂水平包括血清高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、三酰甘油(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、总胆固醇(total cholesterol,TC)进行检测;对肝功能变化包括谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)进行测定分析;对脂肪生成相关基因表达变化包括脂肪酸合成酶(fatty acid synthase,FAS)、脂肪细胞脂结合蛋白(adipocyte lipid binding protein,AP2)、脂蛋白酯酶(lipoprotein lipase,LPL)进行测定分析。结果:治疗后,各组大鼠的体质量、Lee′s指数、体脂含量、血清ALT、FAS水平、TC、TG和LDL-C均有下降,HDL-C水平明显升高;佩连麻黄方高剂量组和模型组在体脂含量和体质量方面比较,差异具有统计学意义(P<0.05);高剂量组血清ALT、FAS水平明显低于模型组(P<0.05);与模型组相比,高剂量组血清血脂各指标水平显著改善(P<0.05);和模型组相比,佩连麻黄方治疗组AP2、FAS以及LPL表达水平明显降低,差异具有统计学意义(P<0.05)。结论:佩连麻黄方能够对单纯性肥胖大鼠起到减肥效果,而且能改善肝功能和高血脂症,调节脂代谢紊乱。
Objective:To investigate the effect of Peilian Mahuang Prescription on the body weight of rats with simple obesity and its effects on improving liver function,lowering blood lipid levels and regulating lipid metabolism disorder.Methods:Rats were randomly divided into model group,control group,Pelian Mahuang Prescription low-dose treatment group,medium-dose treatment group and high-dose treatment group.An obese rat model was prepared,and the body length,body weight,Lee′s index,and body fat content of the rats were measured after 8 weeks of administration of the drug to the rats.The blood lipid levels include high density lipoprotein cholesterol(HDL-C),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),total cholesterol(total).Cholesterol,TC) were measured.The Changes in liver function including alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were measured and analyzed.The expression of genes involved in adipogenesis including fatty acid synthase(FAS) and adipocyte lipid binding protein(AP2) and lipoprotein lipase(LPL) were measured and analyzed.Results:After treatment,the body weight,Lee′s index,body fat content,serum ALT,FAS level,TC,TG and LDL-C levels of rats decreased,while HDL-C levels increased significantly.There were significant differences in body weight and body weight between high-dose treatment group and model group(P<0.05).The levels of serum ALT and FAS in the high dose group were significantly lower than those in the model group(P<0.05);compared with the model group,the changes of serum lipid levels in the high dose group were significantly different(P<0.05);compared with the model group,the expression levels of AP2,FAS and LPL in the treatment group were significantly lower(P<0.05).Conclusion:Peilian Mahuang prescription can reduce weight in rats with simple obesity,improve liver function and hyperlipidemia,and regulate lipid metabolism disorders.
Objective:To investigate the effect of Peilian Mahuang Prescription on the body weight of rats with simple obesity and its effects on improving liver function,lowering blood lipid levels and regulating lipid metabolism disorder.Methods:Rats were randomly divided into model group,control group,Pelian Mahuang Prescription low-dose treatment group,medium-dose treatment group and high-dose treatment group.An obese rat model was prepared,and the body length,body weight,Lee′s index,and body fat content of the rats were measured after 8 weeks of administration of the drug to the rats.The blood lipid levels include high density lipoprotein cholesterol(HDL-C),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),total cholesterol(total).Cholesterol,TC) were measured.The Changes in liver function including alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were measured and analyzed.The expression of genes involved in adipogenesis including fatty acid synthase(FAS) and adipocyte lipid binding protein(AP2) and lipoprotein lipase(LPL) were measured and analyzed.Results:After treatment,the body weight,Lee′s index,body fat content,serum ALT,FAS level,TC,TG and LDL-C levels of rats decreased,while HDL-C levels increased significantly.There were significant differences in body weight and body weight between high-dose treatment group and model group(P<0.05).The levels of serum ALT and FAS in the high dose group were significantly lower than those in the model group(P<0.05);compared with the model group,the changes of serum lipid levels in the high dose group were significantly different(P<0.05);compared with the model group,the expression levels of AP2,FAS and LPL in the treatment group were significantly lower(P<0.05).Conclusion:Peilian Mahuang prescription can reduce weight in rats with simple obesity,improve liver function and hyperlipidemia,and regulate lipid metabolism disorders.
引文
[1]吴巧敏,倪海祥,卢笑,等.山奈酚延缓自发性肥胖2型糖尿病小鼠肾损伤研究[J].心脑血管病防治,2017,17(1):19-22.
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[9]刘鑫,邹中兰,梅全慧,等.射干麻黄汤对慢性哮喘大鼠缺氧诱导因子-1α、血管内皮生长因子表达及气道重塑的影响[J].中国实验方剂学杂志,2012,18(8):190-195.
[10]王艳宏,王秋红,夏永刚,等.麻黄化学拆分组分的性味药理学评价——麻黄化学拆分组分“辛宣苦泄”平喘作用的研究[J].中国实验方剂学杂志,2011,17(24):136-139.
[11]马建,孙丹,赵娜.佩连麻黄方对单纯性肥胖大鼠减肥作用的实验研究[J].中国中医药科技,2014,21(2):132-134.
[12]马建,余海燕,赵娜,等.佩连麻黄方治疗单纯性肥胖的临床研究[J].中医药信息,2014,31(1):46-49.
[13]金军英,张雪,霍平.单纯性肥胖成人同型半胱氨酸水平及其代谢途径与冠心病关系[J].标记免疫分析与临床,2016,23(11):1291-1293.
[14]杨惠林,毛小龙,冉景锐,等.土家族200例单纯肥胖儿童血清HCY水平调查研究[J].临床检验杂志(电子版),2017,6(2):153-155.
[15]高磊,孔显娟,石现.电针和穴位埋线对单纯性肥胖大鼠脂质代谢基因PPAR-γ mRNA表达及相关脂代谢酶的影响[J].中国针灸,2011,31(6):535-538.
[16]刘姚,陈婷婷,傅凌韵,等.青钱柳多糖对高脂血症小鼠脂肪酸合成酶(FAS)表达影响[J].江西农业大学学报,2013,35(2):392-397.
[17]卢环宇,韦梦影,张更,等.脂肪酸合酶与脂肪酸代谢及细胞因子的关系[J].细胞与分子免疫学杂志,2012,28(11):1226-1228.
[18]马爽,刘莉,李岩溪,等.肥胖和肥胖抵抗大鼠脂肪组织PPARγ和aP2基因表达的研究[J].卫生研究,2009,38(2):163-165.
[19]李娟娥,孙文,秦灵灵,等.桂皮醛对3T3-L1前脂肪细胞诱导分化的作用及其机制研究[J].中华中医药学刊,2015,33(8):1916-1918.
[20]易娟,邓慧君,曹进.绿茶和红茶多酚对大鼠脂肪分化相关基因表达影响的比较研究[J].营养学报,2007,29(6):582-586.
[2]于娜,左加成,穆倩倩,等.降糖消渴颗粒对自发性糖尿病KKAy小鼠肾脏JNK信号通路的影响[J].中华中医药杂志,2016,31(5):1801-1804.
[3]闫小光,孙文,刘铜华,等.止消通脉宁对糖尿病小鼠肾组织TGF-β1、CTGF、BMP7和基因表达的影响[J].北京中医药,2013,32(6):462-466.
[4]包书茵,韩淑英,朝日雅,等.沙蓬粗寡糖对GK大鼠肝、肾保护作用及机制探讨[J].中国药理学通报,2018,34(1):147-148.
[5]石书梅,张爱平,丁尧海,等.以急性肾损伤为首发症状的自发性肾静脉血栓二例[J].中华肾脏病杂志,2011,27(3):160.
[6]陈达民.肝硬化患者自发性细菌性腹膜炎后的肾损伤:发生率、临床过程、预测因素及预后[J].国际消化病杂志,1995,15(4):240-241.
[7]唐荣,周巧玲,Veeraragoo Pouranan,等.冬虫夏草对自发性高血压大鼠klotho表达及氧化应激的影响[J].肾脏病与透析肾移植杂志,2010,19(4):338-343.
[8]王宇明,汤影子,邓国宏,等.肝衰竭合并症研究进展[J].传染病信息,2010,23(2):68-71.
[9]刘鑫,邹中兰,梅全慧,等.射干麻黄汤对慢性哮喘大鼠缺氧诱导因子-1α、血管内皮生长因子表达及气道重塑的影响[J].中国实验方剂学杂志,2012,18(8):190-195.
[10]王艳宏,王秋红,夏永刚,等.麻黄化学拆分组分的性味药理学评价——麻黄化学拆分组分“辛宣苦泄”平喘作用的研究[J].中国实验方剂学杂志,2011,17(24):136-139.
[11]马建,孙丹,赵娜.佩连麻黄方对单纯性肥胖大鼠减肥作用的实验研究[J].中国中医药科技,2014,21(2):132-134.
[12]马建,余海燕,赵娜,等.佩连麻黄方治疗单纯性肥胖的临床研究[J].中医药信息,2014,31(1):46-49.
[13]金军英,张雪,霍平.单纯性肥胖成人同型半胱氨酸水平及其代谢途径与冠心病关系[J].标记免疫分析与临床,2016,23(11):1291-1293.
[14]杨惠林,毛小龙,冉景锐,等.土家族200例单纯肥胖儿童血清HCY水平调查研究[J].临床检验杂志(电子版),2017,6(2):153-155.
[15]高磊,孔显娟,石现.电针和穴位埋线对单纯性肥胖大鼠脂质代谢基因PPAR-γ mRNA表达及相关脂代谢酶的影响[J].中国针灸,2011,31(6):535-538.
[16]刘姚,陈婷婷,傅凌韵,等.青钱柳多糖对高脂血症小鼠脂肪酸合成酶(FAS)表达影响[J].江西农业大学学报,2013,35(2):392-397.
[17]卢环宇,韦梦影,张更,等.脂肪酸合酶与脂肪酸代谢及细胞因子的关系[J].细胞与分子免疫学杂志,2012,28(11):1226-1228.
[18]马爽,刘莉,李岩溪,等.肥胖和肥胖抵抗大鼠脂肪组织PPARγ和aP2基因表达的研究[J].卫生研究,2009,38(2):163-165.
[19]李娟娥,孙文,秦灵灵,等.桂皮醛对3T3-L1前脂肪细胞诱导分化的作用及其机制研究[J].中华中医药学刊,2015,33(8):1916-1918.
[20]易娟,邓慧君,曹进.绿茶和红茶多酚对大鼠脂肪分化相关基因表达影响的比较研究[J].营养学报,2007,29(6):582-586.