胶红酵母Rhodotorula mucilaginosa CICC 33013胞外多糖的分离纯化及抗氧化活性研究
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摘要
研究胶红酵母Rhodotorula mucilaginosa CICC 33013菌株胞外多糖的初级结构与抗氧化活性。分离纯化得到胞外多糖的单一组分REPS2-A,分析其分子质量分布及自由基清除活性。结果表明,多糖组分REPS2-A的分子质量为7. 125×106Da,质量浓度为8 mg/m L时,DPPH自由基清除率为49. 1%,ABTS自由基清除率为51. 2%,还原力为0. 352。该文为了解胶红酵母菌的胞外多糖结构与功能提供了新的数据依据,为后续胶红酵母菌胞外多糖的构-效关系研究提供了实验基础。
The primary structures and antioxidant activities of Rhodotorula mucilaginosa extracellular polysaccharides were studied in this paper. A single component REPS2-A in extracellular polysaccharides was obtained by isolation and purification,followed by measuring its molecular weight distribution and free radical scavenging activities.The results showed that the molecular weight of REPS2-A was 7. 125 × 106 Da. Moreover,the scavenging rates of DPPH and ABTS free radicals by 8 mg/m L REPS2-A were 49. 1% and 51. 2%,respectively,and the reducing power was 0. 352. This paper provides a new data basis for understanding the structures and functions of extracellular polysaccharides of R. mucilaginosa,which provides an experimental basis for subsequent studies on correlations between their structures and activities.
The primary structures and antioxidant activities of Rhodotorula mucilaginosa extracellular polysaccharides were studied in this paper. A single component REPS2-A in extracellular polysaccharides was obtained by isolation and purification,followed by measuring its molecular weight distribution and free radical scavenging activities.The results showed that the molecular weight of REPS2-A was 7. 125 × 106 Da. Moreover,the scavenging rates of DPPH and ABTS free radicals by 8 mg/m L REPS2-A were 49. 1% and 51. 2%,respectively,and the reducing power was 0. 352. This paper provides a new data basis for understanding the structures and functions of extracellular polysaccharides of R. mucilaginosa,which provides an experimental basis for subsequent studies on correlations between their structures and activities.
引文
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[7] JIN L G,YOU J C,GUO Q S,et al. Study on phenol-sulfuric acid method for determination of polysaccharide content in Pleurotus eryngii[J]. Food Science,2008,29(12):555-558.
[8] CHI Z,ZHAO S. Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain[J]. Enzyme and Microbial Technology,2003,33(2):206-211.
[9] PENG Q,LYU X,XU Q,et al. Isolation and structural characterization of the polysaccharide LRGP1 from Lycium ruthenicum[J]. Carbohydrate Polymers,2012,90(1):95-101.
[10] LIC,FU X,LUO F,et al. Effects of maltose on stability and rheological properties of orange oil-in-water emulsion formed by OSA modified starch[J]. Food Hydrocolloids,2013,32(1):79-86.
[11] WANG R,CHEN P,JIA F,et al. Characterization and antioxidant activities of polysaccharides from Panax japonicus C. A. Meyer[J]. Carbohydrate Polymers,2012,88(4):1 402-1 406.
[12] ZHU K,ZHOU H,QIAN H. Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates(WGPH)prepared with alcalase[J]. Process Biochemistry,2006,41(6):1 296-302.
[13] CHENG H,FENG S,JIA X,et al. Structural characterization and antioxidant activities of polysaccharides extracted from Epimedium acuminatum[J]. Carbohydrate Polymers,2013,92(1):63-68.
[14] LI Q,YU N,WANG Y,et al. Extraction optimization of Bruguiera gymnorrhiza polysaccharides with radical scavenging activities[J]. Carbohydrate Polymers,2013,96(1):148-155.
[15]景永帅,吴兰芳,王乾,等.远志多糖提取工艺优化及其抗氧化活性研究[J].食品与机械,2016,32(5):152-156.
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[17]李彬,陈向楠,张建法,等.产胞外多糖菌株的筛选及胞外多糖结构分析[J].生物技术通报,2016,32(5):165-171.
[18] LIANG L,WU X,ZHU M,et al. Chemical composition,nutritional value,and antioxidant activities of eight mulberry cultivars from China[J]. Pharmacognosy Magazine,2012,8(31):215-224.
[19] ZHANG A Q,XIAO N N,DENG Y L,et al. Purification and structural investigation of a water-soluble polysaccharide from Flammulina velutipes[J]. Carbohydrate Polymers,2012,87(3):2 279-2 283.
[20] IRSHAD M,ZAFARYAB M,SINGH M,et al. Comparative analysis of the antioxidant activity of Cassia fistula extracts[J]. International Journal of Medicinal Chemistry,2012,2012:1-6.
[21]潘乔丹,黄元河,唐海燕,等.赤苍藤和密蒙花多糖的含量测定及抗氧化研究[J].食品研究与开发,2016,37(22):6-9.
[22] ZOU C,DU Y,LI Y,et al. Preparation of lacquer polysaccharide sulfates and their antioxidant activity in vitro[J]. Carbohydrate Polymers,2008,73(2):322-331.
[23] HU C,ZHANG Y,KITTS D D. Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. Henonis leaf extract in vitro[J]. Journal of Agricultural&Food Chemistry,2000,48(8):3 170-3 176.
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