摘要
目的:研究NADPH氧化酶抑制剂夹竹桃麻素(Apocynin)对双酚a(Bisphenol a,BPA)诱导的成年雄性小鼠精子损伤过程中的作用。方法:成年雄性小鼠给以BPA刺激,给以Apocynin(1 mg/kg/day和10 mg/kg/day,分别处理7 day)进行治疗,观察其对BPA诱导的成年雄性小鼠精子损伤的作用。结果:BPA处理组小鼠附睾精子数目和活力明显降低,血清中睾酮和黄体生成素水平明显减少;Apocynin治疗明显增加BPA处理组小鼠附睾中精子数目和活力,但是对血清中睾酮和黄体生成素水平没有明显影响。另外,BPA处理组小鼠睾丸中丙二醛(Malonaldehyde,MDA)水平明显增加;Apocynin治疗明显抑制了BPA处理组小鼠睾丸中MDA水平。结论:NADPH氧化酶抑制剂Apocynin减轻BPA诱导的成年雄性小鼠精子损伤。
Objective: To investigate the effect of treatment with apocynin(one NADPH oxidase inhibitor) against biophenol a(BPA)-induced injury in sperms of male adult mice. Methods: Male adult mice were stimulated with BPA and simultaneously treated with apocynin(1 mg/kg, 10 mg/kg) for 7 days. Testis and serum were collected for biochemical analysis. Results: Exposure to BPA led to reduced number and motility of sperms of epididymises and serum levels of testosterone and luteinizing hormone. Treatment of BPA mice with apocynin enhanced the number and motility of sperms of epididymises, but did not affect the serum levels of testosterone and luteinizing hormone. In addition, exposure to BPA led to enhanced formation of malondialdehyde in testis, and treatment of BPA mice with apocynin reduced formation of malondialdehyde in testis. Conclusion: Apocynin attenuated BPA-induced injury in sperms of male adult mice.
引文
[1]Srivastava S,Gupta P,Chandolia A,et al.Bisphenol A:a threat to human health?[J].J Environ Health,2015,77(6):20-26
[2]Sekizawa J.Low-dose effects of bisphenol A:a serious threat to human health?[J].J Toxicol Sci,2008,33(4):389-403
[3]Goldstone AE,Chen Z,Perry MJ,et al.Urinary bisphenol A and semen quality,the LIFE Study[J].Reprod Toxicol,2015,51:7-13
[4]Yang YJ,Hong YP,Chae SA.Reduction in semen quality after mixed exposure to bisphenol A and isobutylparaben in utero and during lactation periods[J].Hum Exp Toxicol,2015
[5]Meeker JD,Ehrlich S,Toth TL,et al.Semen quality and sperm DNA damage in relation to urinary bisphenol A among men from an infertility clinic[J].Reprod Toxicol,2010,30(4):532-539
[6]Wisniewski P,Romano RM,Kizys MM,et al.Adult exposure to bisphenol A(BPA)in Wistar rats reduces sperm quality with disruption of the hypothalamic-pituitary-testicular axis[J].Toxicology,2015,329:1-9
[7]Manfo FP,Jubendradass R,Nantia EA,et al.Adverse effects of bisphenol A on male reproductive function[J].Rev Environ Contam Toxicol,2014,228:57-82
[8]Xin F,Jiang L,Liu X,et al.Bisphenol A induces oxidative stress-associated DNA damage in INS-1 cells[J].Mutat Res Genet Toxicol Environ Mutagen,2014,769:29-33
[9]Khan S,Beigh S,Chaudhari BP,et al.Mitochondrial dysfunction induced by Bisphenol A is a factor of its hepatotoxicity in rats[J].Environ Toxicol,2015
[10]Moustafa MH,Sharma RK,Thornton J,et al.Relationship between ROS production,apoptosis and DNA denaturation in spermatozoa from patients examined for infertility[J].Hum Reprod,2004,19(1):129-138
[11]Omu AE,Al-Azemi MK,Kehinde EO,et al.Indications of the mechanisms involved in improved sperm parameters by zinc therapy[J].Med Princ Pract,2008,17(2):108-116
[12]Armstrong JS,Bivalacqua TJ,Chamulitrat W,et al.A comparison of the NADPH oxidase in human sperm and white blood cells[J].Int J Androl,2002,25(4):223-229
[13]Zhang Y,Lv Y,Liu YJ,et al.Hyperbaric oxygen therapy in rats attenuates ischemia-reperfusion testicular injury through blockade of oxidative stress,suppression of inflammation,and reduction of nitric oxide formation[J].Urology,2013,82(2):489 e489-489 e415
[14]Sangai NP,Verma RJ,Trivedi MH.Testing the efficacy of quercetin in mitigating bisphenol A toxicity in liver and kidney of mice[J].Toxicol Ind Health,2014,30(7):581-597
[15]Erden ES,Motor S,Ustun I,et al.Investigation of Bisphenol A as an endocrine disruptor,total thiol,malondialdehyde,and C-reactive protein levels in chronic obstructive pulmonary disease[J].Eur Rev Med Pharmacol Sci,2014,18(22):3477-3483
[16]Wang Q,Zhao XF,Ji YL,et al.Mitochondrial signaling pathway is also involved in bisphenol A induced germ cell apoptosis in testes[J].Toxicol Lett,2010,199(2):129-135
[17]Qian W,Wang Y,Zhu J,et al.The toxic effects of Bisphenol A on the mouse spermatocyte GC-2 cell line:the role of the Ca(2+)-calmodulin-Ca(2+)/calmodulin-dependent protein kinase II axis[J].J Appl Toxicol,2015,35(11):1271-1277
[18]Handa RJ,Weiser MJ.Gonadal steroid hormones and the hypothalamo-pituitary-adrenal axis[J].Front Neuroendocrinol,2014,35(2):197-220
[19]Fernandez-Guasti A,Fiedler JL,Herrera L,et al.Sex,stress,and mood disorders:at the intersection of adrenal and gonadal hormones[J].Horm Metab Res,2012,44(8):607-618
[20]Evuarherhe O,Leggett J,Waite E,et al.Reversal of the hypothalamo-pituitary-adrenal response to oestrogens around puberty[J].J Endocrinol,2009,202(2):279-285