龙牧苜蓿种质资源耐混合苏打盐碱能力综合分析
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摘要
以5种龙牧苜蓿种子为试验材料,研究不同浓度(10,20,30,40和50mmol·L~(-1))混合苏打盐碱(NaHCO_3∶Na_2CO_3=9∶1)对龙牧系列苜蓿种子萌发影响。结果表明:苜蓿种子在不同混合苏打盐碱浓度下其发芽率(Germination rate,GR)、发芽势(Germination potential,GP)、发芽指数(Germination index,GI)、活力指数(Vigor index,VI)、干重(Dry weight,DW)、鲜重(Fresh weight,FW)、含水量(Water content,WC)、胚芽长(Embryo length,EL)、胚根长(Radicle length,RL)等9项指标均表现出不同的变化趋势,对9项指标的主成分分析共筛选出GR,GP,GI,VI,DW,WC,EL等指标与龙牧苜蓿品种的抗盐碱性有关,利用隶属函数、权重系数和综合评价D值分析可知,5种龙牧苜蓿品种耐盐碱性强弱依次为:龙牧807>龙牧808>龙牧806>龙牧803>龙牧801。
The effects of mixed soda saline-alkali(NaHCO_3∶Na_2CO_3 =9∶1)at different concentrations(10,20,30,40 and 50 mmol·L~(-1))on the germination of five Longmu alfalfa seeds were studied.The results showed that the germination rate,germination potential,germination index,vigor index,dry weight,fresh weight,water content,embryo length,radicle length of alfalfa seeds showed different trends under different mixed soda saline-alkali concentrations.Through principal component analysis of nine indicators,GR,GP,GI,VI,FW,WC and EL indicators were screened out to be related to salt and alkali tolerance of Longmu alfalfa varieties.According to the analysis of membership function,weight coefficient and D value of comprehensive evaluation,the salt and alkali tolerance of five longmu alfalfa varieties were in the order of LM 807>LM 808>LM 806>LM 803>LM 801.
The effects of mixed soda saline-alkali(NaHCO_3∶Na_2CO_3 =9∶1)at different concentrations(10,20,30,40 and 50 mmol·L~(-1))on the germination of five Longmu alfalfa seeds were studied.The results showed that the germination rate,germination potential,germination index,vigor index,dry weight,fresh weight,water content,embryo length,radicle length of alfalfa seeds showed different trends under different mixed soda saline-alkali concentrations.Through principal component analysis of nine indicators,GR,GP,GI,VI,FW,WC and EL indicators were screened out to be related to salt and alkali tolerance of Longmu alfalfa varieties.According to the analysis of membership function,weight coefficient and D value of comprehensive evaluation,the salt and alkali tolerance of five longmu alfalfa varieties were in the order of LM 807>LM 808>LM 806>LM 803>LM 801.
引文
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[2]Wang C,Ma B,Yan X,et al.Yields of alfalfa varieties with different fall-dormancy levels in a temperate environment[J].Agron J.2009,101(5):1146-1152
[3]李波,于海龙.苏打盐碱胁迫下不同来源苜蓿品种抗盐性综合评价[J].草地学报,2017,25(1):204-208
[4]刘卓,王志锋,于洪柱,等.不同苜蓿品种种子萌发期耐盐性的研究[J].云南农业大学学报(自然科学版),2010,25(3):358-363
[5]陈小芳,于德花,宁凯,等.盐胁迫下苜蓿种质资源萌发特性综合评价[J].草地学报,2017,25(5):1115-1125
[6]张寒,潘香逾,王秀华,等.苜蓿萌发期耐盐性综合评价与耐盐种质筛选[J].草地学报,2018,26(3):666-672
[7]李波,陈雪梅,于海龙,等.混合盐碱胁迫对苜蓿种子萌发特性的影响[J].江苏农业科学,2015,43(8):221-225
[8]张利霞,常青山,侯小改,等.不同钠盐胁迫对夏枯草种子萌发特性的影响[J].草业学报,2015,24(3):177-186
[9]卢艳敏,苏长青,李会芬.不同盐胁迫对白三叶种子萌发及幼苗生长的影响[J].草业学报,2013,22(4):123-129
[10]刘自刚,沈冰,张雁.桔梗种子萌发对低温、干旱及互作胁迫的响应[J].生态学报,2013,33(8):2615-2622
[11]田小霞,毛培春,孟林,等.无芒雀麦苗期耐盐指标筛选及耐盐性综合评价[J].干旱区资源与环境,2017,31(10):156-161
[12]朱世杨,张小玲,罗天宽,等.花椰菜种质资源萌发期耐盐性综合评价[J].核农学报,2012,26(2):380-390
[13]沈振荣,杨万仁,徐秀梅.不同盐分胁迫对苜蓿种子萌发的影响[J].种子,2006,25(4):34-37
[14]敬雪敏,刘香萍.盐胁迫与低温胁迫对紫花苜蓿种子发芽的协同影响[J].黑龙江畜牧兽医,2018(12):169-171,250
[15]古丽内尔·亚森,杨瑞瑞,曾幼玲.混合盐碱胁迫对灰绿藜种子萌发的影响[J].生态学杂志,2014,33(1):76-82
[16]胡凯凤,孙丽芳,邓杰,等.苏打碱胁迫对玉米种子萌发的影响[J].种子,2016,35(6):41-45
[17]王素平,郭世荣,李璟,等.盐胁迫对黄瓜幼苗根系生长和水分利用的影响[J].应用生态学报,2006,17(10):1883-1888
[18]翁亚伟.小麦幼苗对盐旱复合胁迫的响应机理[D].南京:南京农业大学,2016:3-4
[19]王素平.多胺对黄瓜幼苗耐盐性调控机理的研究[D].南京:南京农业大学,2007:3
[20]田小霞,毛培春,张琳,等.苜蓿属植物苗期耐盐指标筛选及耐盐性综合评价[J].植物资源与环境学报,2018,27(2):46-56
[21]董志刚,程智慧.番茄品种资源芽苗期和幼苗期的耐盐性及耐盐指标评价[J].生态学报,2009,29(3):1348-1355
[22]贾亚雄,李向林,袁庆华,等.披碱草属野生种质资源苗期耐盐性评价及相关生理机制研究[J].中国农业科学,2008,41(10):2999-3007