SULT2A1、miR-18a蛋白在子宫肌瘤组织中的表达及意义
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摘要
目的探讨硫酸基转移酶2A1(SULT2A1)、miR-18a在子宫肌瘤组织中的表达及其意义。方法选取收集的90例子宫肌瘤术后组织(肌瘤组)、45例因子宫脱垂实施手术治疗的子宫肌层组织标本(对照组),采用免疫组化染色检测2组的SULT2A1蛋白、应用PCR检测2组标本中的miR-18a表达,并分析SULT2A1、miR-18a表达与子宫肌瘤数目、肌瘤大小的关系。结果肌瘤组中的SULT2A1蛋白阳性表达率为47.78%低于对照组的71.11%,差异有统计学意义(P<0.05);肌瘤组中的miR-18a蛋白阳性表达率40.00%低于对照组的75.56%,差异有统计学意义(P<0.05);单发子宫肌瘤组织中的SULT2A1蛋白阳性表达率显著的高于多发子宫肌瘤组织(P<0.05);单发子宫肌瘤组织、肌瘤直径≤5 cm的子宫肌瘤组织中的miR-18a蛋白阳性表达率显著的高于多发子宫肌瘤组织、肌瘤直径>5 cm的子宫肌瘤组织(P<0.05)。结论 SULT2A1、miR-18a蛋白在子宫肌瘤组织中的表达下调,并且与肌瘤数目、大小具有一定的相关性。
Objective To explore the the expression and significance of sulfotransferases 2 A1(SULT2 A1) and miR18 a in uterus myomas tissues.Methods Ninety cases of myometrial tissues after myomectomy were enrolled as fibroid group and forty-five cases of myometrial tissues after surgery due to uterine prolapse were enrolled as control group. Immunohistochemical staining and PCR were used to detect the expression levels of SULT2 A1 protein and tmiR18 a in both groups, respectively. Moreover, the correlation between the expressions of miR18 a, SULT2 A1 and the number,size of uterus myomas was analyzed. Results The positive expression rate of SULT2 A1 protein(47.78%) and miR18 a protein(40.00%) in fibroid group were significantly lower than those [(71.11%) and(75.56%),respectively] in control group(P<0.05). Moreover, the positive expression rate of SULT2 A1 protein in single uterus myomas tissues was significantly higher than that in multiple uterus myomas tissues(P<0.05). And the positive expression rates of miR18 a protein in single uterus myomas and uterus myomas with tumor diameter ≤5 cm were significantly higher than those in multiple uterus myomas and the uterus myomas with tumor diameter>5 cm, respectively(P<0.05).Conclusion The expression levels of SULT2 A1 and miR18 a protein in uterus myomas tissues are down-regulated,which are correlated with the number and the size of fibroids to some extent.
Objective To explore the the expression and significance of sulfotransferases 2 A1(SULT2 A1) and miR18 a in uterus myomas tissues.Methods Ninety cases of myometrial tissues after myomectomy were enrolled as fibroid group and forty-five cases of myometrial tissues after surgery due to uterine prolapse were enrolled as control group. Immunohistochemical staining and PCR were used to detect the expression levels of SULT2 A1 protein and tmiR18 a in both groups, respectively. Moreover, the correlation between the expressions of miR18 a, SULT2 A1 and the number,size of uterus myomas was analyzed. Results The positive expression rate of SULT2 A1 protein(47.78%) and miR18 a protein(40.00%) in fibroid group were significantly lower than those [(71.11%) and(75.56%),respectively] in control group(P<0.05). Moreover, the positive expression rate of SULT2 A1 protein in single uterus myomas tissues was significantly higher than that in multiple uterus myomas tissues(P<0.05). And the positive expression rates of miR18 a protein in single uterus myomas and uterus myomas with tumor diameter ≤5 cm were significantly higher than those in multiple uterus myomas and the uterus myomas with tumor diameter>5 cm, respectively(P<0.05).Conclusion The expression levels of SULT2 A1 and miR18 a protein in uterus myomas tissues are down-regulated,which are correlated with the number and the size of fibroids to some extent.
引文
1 Brar R,Skervin A,El-Sakka K,et al.Successful operative treatment of uterine leiomyoma with extensive intravenous extension to the IVC,right heart,and pulmonary arteries.Acta Chirurgica Belgica,2018,25:1-4.
2 Vasilev N,Nacheva A,Zlatkov B,et al.Uterine leiomyoma and fibroleio- myoma:treatment for non-pregnant women.Akusherstvo iginekologiia,2015,54:1-3.
3 Hrgovic Z,Habek D,Cerkez Habek J,et al.Spontaneous pregnancy during ulipristal acetate treatment of giant uterine leiomyoma.Journal of Clinical Pharmacy and Therapeutics,2018,43:121-123.
4 曹荣,王敏,王丹丹,等.SULT1A1蛋白表达和性激素含量与子宫肌瘤发病相关性的研究.现代肿瘤医学,2014,22:615-618.
5 刘琴,徐明娟.MicroRNA在子宫肌瘤发病机制研究中的现状.中国细胞生物学学报,2015,37:137-142.
6 陈超,李冬华,王满元,等.子宫肌瘤细胞凋亡机制及药物靶点治疗研究要点探析.医学研究杂志,2017,46:8-10.
7 沈东成,徐秋霞,杜洁,等.子宫肌瘤中西医发病机制的研究进展.中华中医药学刊,2015,33:1110-1112.
8 Brar R,Skervin A,El-Sakka K,et al.Successful operative treatment of uterine leiomyoma with extensive intravenous extension to the IVC,right heart,and pulmonary arteries.Acta Chirurgica Belgica,2018,32:1-4.
9 Ko JKY,Cheung VYT.Comment on:Delayed intestinal perforation and vertebral osteomyelitis after high-intensity focused ultrasound treatment for uterine leiomyoma.Obstetrics Gynecology Science,2018,61:177-179.
10 刘易斯,Pankaj Pratap Deo,宋燕,等.硫酸基转移酶2A1蛋白在子宫肌瘤组织的表达及其临床意义研究.中国实用妇科与产科杂志,2016,32:469-472.
11 刘琴,徐明娟.子宫肌瘤差异表达microRNA的定量分析及靶基因鉴定.中国细胞生物学学报,2015,37:671-676.
12 欧阳晨捷.子宫肌瘤的发病机制研究进展.中南医学科学杂志,2016,44:708-711.
13 谢建军,李健和,易利丹,等.子宫肌瘤的发病机制与药物治疗.中国医药导报,2011,8:16-18.
14 张传琪,张怡舜,王敏.子宫肌瘤组织硫酸基转移酶1A3与雌激素受体亚型ERα、ERβ的表达及临床意义研究.中国实用妇科与产科杂志,2017,33:205-208.
2 Vasilev N,Nacheva A,Zlatkov B,et al.Uterine leiomyoma and fibroleio- myoma:treatment for non-pregnant women.Akusherstvo iginekologiia,2015,54:1-3.
3 Hrgovic Z,Habek D,Cerkez Habek J,et al.Spontaneous pregnancy during ulipristal acetate treatment of giant uterine leiomyoma.Journal of Clinical Pharmacy and Therapeutics,2018,43:121-123.
4 曹荣,王敏,王丹丹,等.SULT1A1蛋白表达和性激素含量与子宫肌瘤发病相关性的研究.现代肿瘤医学,2014,22:615-618.
5 刘琴,徐明娟.MicroRNA在子宫肌瘤发病机制研究中的现状.中国细胞生物学学报,2015,37:137-142.
6 陈超,李冬华,王满元,等.子宫肌瘤细胞凋亡机制及药物靶点治疗研究要点探析.医学研究杂志,2017,46:8-10.
7 沈东成,徐秋霞,杜洁,等.子宫肌瘤中西医发病机制的研究进展.中华中医药学刊,2015,33:1110-1112.
8 Brar R,Skervin A,El-Sakka K,et al.Successful operative treatment of uterine leiomyoma with extensive intravenous extension to the IVC,right heart,and pulmonary arteries.Acta Chirurgica Belgica,2018,32:1-4.
9 Ko JKY,Cheung VYT.Comment on:Delayed intestinal perforation and vertebral osteomyelitis after high-intensity focused ultrasound treatment for uterine leiomyoma.Obstetrics Gynecology Science,2018,61:177-179.
10 刘易斯,Pankaj Pratap Deo,宋燕,等.硫酸基转移酶2A1蛋白在子宫肌瘤组织的表达及其临床意义研究.中国实用妇科与产科杂志,2016,32:469-472.
11 刘琴,徐明娟.子宫肌瘤差异表达microRNA的定量分析及靶基因鉴定.中国细胞生物学学报,2015,37:671-676.
12 欧阳晨捷.子宫肌瘤的发病机制研究进展.中南医学科学杂志,2016,44:708-711.
13 谢建军,李健和,易利丹,等.子宫肌瘤的发病机制与药物治疗.中国医药导报,2011,8:16-18.
14 张传琪,张怡舜,王敏.子宫肌瘤组织硫酸基转移酶1A3与雌激素受体亚型ERα、ERβ的表达及临床意义研究.中国实用妇科与产科杂志,2017,33:205-208.