基于IGS和Histone基因的我国芒果镰孢菌遗传多样性分析
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摘要
[目的]研究我国芒果镰孢菌的遗传多样性。[方法]从云南、广西、海南等芒果主产区芒果花序上分离得到芒果镰孢菌,采用IGS、Histone 2个基因片段对芒果镰孢菌进行遗传多样性分析。[结果]我国芒果镰孢菌具有多个谱系,致病芒果镰孢菌为单系群。[结论]我国芒果镰孢菌具有较高的遗传多样性。
[Objective] To study genetic diversity of Fusarium mangiferae in China. [Method] Genetic diversity among F. mangiferae from Hainan,Guangxi and Yunnan provinces using the partial IGS and Histone gene sequences were analyzed. The strains were isolated from symptomatic and non-symptomatic inflorescences and twigs. [Result]It was indicated that high genetic variation existed among those strains in China,while the pathogenetic isolates were monophyletic. [Conclusion] Fusarium mangiferae in China has high genetic diversity.
[Objective] To study genetic diversity of Fusarium mangiferae in China. [Method] Genetic diversity among F. mangiferae from Hainan,Guangxi and Yunnan provinces using the partial IGS and Histone gene sequences were analyzed. The strains were isolated from symptomatic and non-symptomatic inflorescences and twigs. [Result]It was indicated that high genetic variation existed among those strains in China,while the pathogenetic isolates were monophyletic. [Conclusion] Fusarium mangiferae in China has high genetic diversity.
引文
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[13]GLASS N L,DONALDSON G C.Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes[J].Applied and environmental microbiology,1995,61:1323-1330.
[2]MARASAS W F O,PLOETZ R C,WINGFIELD M J,et al.Mango malformation disease and the associated Fusarium species[J].Phytopathology,2006,96:667-672.
[3]CHAKRABARTI D K.Mango malformation[M].Uttar Pradesh,India:Springer,2011:1-148.
[4]WATT G.A dictionary of the economic products of India[J].Mango tree,1891,5(1):149.
[5]SUMMANWAR A S,RAYCHAUDHURI S P,PATHAK S C.Association of fungus Fusarium moniliforme Sheld.with the malformation in mango[J].Indian phytopathology,1966,19:227-228.
[6]BRITZ H,STEENKAMP E T,COUTINHO T A,et al.Two new species of Fusarium section Liseola associated with mango malformation[J].Mycologia,2002,94:722-730.
[7]OTERO-COLINA G,RODRGUEZ-ALVARADO G,FERNNDEZPAVA S,et al.Identification and characterization of a novel etiological agent of mango malformation disease in Mexico,Fusarium mexicanum sp.nov[J].Phytopathology,2010,100:1176-1184.
[8]LIMA C S,PFENNING L H,COSTA S S,et al.A new Fusarium lineage within the Gibberella fujikuroi species complex is the main causal agent of mango malformation disease in Brazil[J].Plant pathology,2009,58:33-42.
[9]杨顺锦,詹儒林,柳凤,等.金沙江干热河谷地区芒果畸形病的病原菌[J].菌物学报,2011,30(1):27-31.
[10]PLOETZ R C,ZHENG Q,VAZQUEZ A,et al.Current status and impact of mango malformation in Egypt[J].International journal of pest management,2002,48:279-285.
[11]LEE S B,TAYLOR J W.Isolation of DNA from fungal mycelial and single spores[M]//INNIS M A,GELFAND D H,SNINSKY J J,et al.PCR protocols:A guide to methods and applications.New York:Academic Press,1990:283-287.
[12]O’DONNELL K,GUEIDAN C,SINK S,et al.A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex[J].Fungal genetics and biology,2009,46:936-948.
[13]GLASS N L,DONALDSON G C.Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes[J].Applied and environmental microbiology,1995,61:1323-1330.