激素浸种对滇青冈苗木生长和生物量积累的影响
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摘要
【目的】分析不同浓度激素组合浸种对滇青冈(Cyclobalanopsis glaucoides)苗木生长、生物量积累和水含率的影响,为培育滇青冈壮苗提供参考依据。【方法】采用L16(43)正交设计开展不同浓度赤霉素(GA3)和6-苄氨基腺嘌呤(6-BA)组合浸泡4个滇青冈家系种子(家系1～家系4)试验,分析各处理组合滇青冈家系苗木的地径、苗高及根、茎、叶、全株生物量和水含率差异。【结果】以1.5 g/L GA3+25.0 mg/L 6-BA浸泡滇青冈家系1种子,其苗木的地径最粗,为2.39 mm;以1.5 g/L GA3浸泡滇青冈家系2种子,其苗木的苗高最高,为14.3 cm;以1.5 g/L GA3+25.0 mg/L 6-BA浸泡家系1种子及以2.5 g/L GA3+50.0 mg/L 6-BA浸泡家系2种子,其苗木根和叶的生物量较高,分别为0.198、0.255 g/株和0.199、0.260 g/株;以2.5 g/L GA3+50.0 mg/L 6-BA浸泡家系2种子,其苗木茎和全株的生物量最高,分别为0.164和0.622 g/株。【结论】家系是影响滇青冈幼苗生长及生物量积累的主要因子;1.5和2.5 g/L GA3浸种可有效促进滇青冈苗木生长和生物量积累,25.0和50.0 mg/L 6-BA浸种可分别促进滇青冈苗木地径生长及全株和茎的生物量积累。
【Objective】In order to provide reference for cultivating sound seedlings of Cyclobalanopsis glaucoides,the effects of hormone combinations with different concentrations soaking seeds on the seedling growth,biomass accumulation and moisture content of C. glaucoides families were analyzed.【Method】The L16(43)orthogonal design was applied to implement the experiment of the gibberellin(GA3)and 6-benzylaminopurine(6-BA)treatment combinations with different concentrations soaking the seeds of four C. glaucoides families(family No.1-4). The differences of basal diameters(BDs),seedling heights(SHs),roots,stems,leaves,biomass of the whole seedling and moisture contents of C. glaucoides families seedlings in different treatment combinations were analyzed.【Result】The treatment combination of 1.5 g/L GA3 and 25.0 mg/L 6-BA soaking the C. glaucoides No.1 family seeds presented the maximum BD(2.39 mm)of the seedlings,the treatment combination of 1.5 g/L GA3 soaking the C. glaucoides No.2 family seeds showed the maximum SH(14.3 cm)of the seedlings,the treatment combination of 1.5 g/L GA3 and 25.0 mg/L 6-BA soaking the No.1 family seeds and the treatment combination of 2.5 g/L GA3 and 50.0 mg/L 6-BA soaking the No.2 family seeds presented the highest biomass of the roots and leaves of the seedlings,being 0.198 and 0.199 g/seedlings for the roots and 0.255 and 0.260g/seedlings for the leaves respectively. The treatment combination of 2.5 g/L GA3 and 50.0 mg/L 6-BA soaking the No.2family seeds showed the maximum biomass of the stem and the whole seedling,being 0.164 and 0.622 g/seedling respectively.【Conclusion】Family is the dominant factor that influences the growth and biomass accumulation of C. glaucoides seedlings. The treatment combination of 1.5 and 2.5 g/L of GA3 solution soaking seeds can promote the growth and biomass accumulation of C. glaucoides seedlings,while the treatment combination of 25.0 and 50.0 mg/L 6-BA solution soaking seeds can facilitate the BD growth of C. glaucoides seedlings and biomass accumulation of the whole seedling and stems.
【Objective】In order to provide reference for cultivating sound seedlings of Cyclobalanopsis glaucoides,the effects of hormone combinations with different concentrations soaking seeds on the seedling growth,biomass accumulation and moisture content of C. glaucoides families were analyzed.【Method】The L16(43)orthogonal design was applied to implement the experiment of the gibberellin(GA3)and 6-benzylaminopurine(6-BA)treatment combinations with different concentrations soaking the seeds of four C. glaucoides families(family No.1-4). The differences of basal diameters(BDs),seedling heights(SHs),roots,stems,leaves,biomass of the whole seedling and moisture contents of C. glaucoides families seedlings in different treatment combinations were analyzed.【Result】The treatment combination of 1.5 g/L GA3 and 25.0 mg/L 6-BA soaking the C. glaucoides No.1 family seeds presented the maximum BD(2.39 mm)of the seedlings,the treatment combination of 1.5 g/L GA3 soaking the C. glaucoides No.2 family seeds showed the maximum SH(14.3 cm)of the seedlings,the treatment combination of 1.5 g/L GA3 and 25.0 mg/L 6-BA soaking the No.1 family seeds and the treatment combination of 2.5 g/L GA3 and 50.0 mg/L 6-BA soaking the No.2 family seeds presented the highest biomass of the roots and leaves of the seedlings,being 0.198 and 0.199 g/seedlings for the roots and 0.255 and 0.260g/seedlings for the leaves respectively. The treatment combination of 2.5 g/L GA3 and 50.0 mg/L 6-BA soaking the No.2family seeds showed the maximum biomass of the stem and the whole seedling,being 0.164 and 0.622 g/seedling respectively.【Conclusion】Family is the dominant factor that influences the growth and biomass accumulation of C. glaucoides seedlings. The treatment combination of 1.5 and 2.5 g/L of GA3 solution soaking seeds can promote the growth and biomass accumulation of C. glaucoides seedlings,while the treatment combination of 25.0 and 50.0 mg/L 6-BA solution soaking seeds can facilitate the BD growth of C. glaucoides seedlings and biomass accumulation of the whole seedling and stems.
引文
鲍雪纤,郑书绿,李莲芳,王慷林,张薇,王文俊,王文静,于国栋.2015.微波辐射和激素浸种对滇青冈种子发芽及幼苗生长的影响[J].种子,34(7):12-15.[Bao X X,Zheng S L,Li L F,Wang K L,Zhang W,Wang W J,Wang W J,Yu G D.2015.Effects of microwave radiation and hormone presoaking seeds on germination and seedling growth of Cyclobalanopsis glaucoides[J].Seed,34(7):12-15.]
鲍雪纤,郑书绿,李莲芳,王慷林,张薇,王文俊,王文静,于国栋,郭樑,苏柠.2016.微波和激素处理对滇青冈幼苗根叶生长及生物量的影响[J].西部林业科学,45(2):83-88.[Bao X X,Zheng S L,Li L F,Wang K L,Zhang W,Wang W J,Wang W J,Yu G D,Guo L,Su N.2016.E-ffects of microwave and hormone treatments on seedling root,leaf and biomass of Cyclobalanopsis glaucoides[J].Journal of West China Forestry Science,45(2):83-88.]
曹建新,张光飞,张磊,李秀华,苏文华.2008.滇青冈幼苗的光合和生长对不同生长光强的适应[J].广西植物,28(1):126-129.[Cao J X,Zhang G F,Zhang L,Li X H,Su W H.2008.Acclimation of photosynthesis and growth to different growth light condition for seedlings of Cyclobalanopsis glaucoides[J].Guihaia,28(1):126-129.]
郭睿达,曾凡锁,詹亚光,李博,何之龙,张桂芹,姚盛智,魏志刚.2013.水曲柳优树子代测定与家系选育技术[J].东北林业大学学报,41(5):1-5.[Guo R D,Zeng F S,Zhan Y G,Li B,He Z L,Zhang G Q,Yao S Z,Wei Z G.2013.Progeny test of superior trees for Fraxinus mandshurica and pedigree breeding[J].Journal of Northeast Forestry University,41(5):1-5.]
李怀春,甘小洪,张泽鹏,张春晓,宋蔺.2015.不同海拔与母树大小对水青树种子生物学特性的影响[J].植物分类与资源学报,37(2):177-183.[Li H C,Gan X H,Zhang Z P,Zhang C X,Song L.2015.Effects of altitudes and the DBH of seed trees on biological characteristics of Tetracentron sinense(Tetracentraceae)seeds[J].Plant Diversity and Resources,37(2):177-183.]
李宁毅,苏胜举,李立.2009.GA3和6-BA对美女樱种子萌发特性及幼苗生长的影响[J].种子,28(11):59-61.[Li NY,Su S J,Li L.2009.Effects of GA3and 6-BA on the seed germination and seedling growth of Verbena hortensis[J].Seed,28(11):59-61.]
李玉文,张朝志,刘永国.2011.3种植物激素不同处理穗条对油茶扦插效果的影响[J].西部林业科学,40(3):59-62.[Li Y W,Zhang C Z,Liu Y G,2011.Effects of different treatments with 3 types of hormones on rooting of cuttage of Camellia oleifera[J].Journal of West China Forestry Science,40(3):59-62.]
刘军,姜景民,陈益泰,董汝湘,姜荣波,孔超.2011.闽楠种子轻基质容器育苗及优良家系选择[J].西北林学院学报,26(6):70-73.[Liu J,Jiang J M,Chen Y T,Dong R X,Jiang R B,Kong C.2011.Cultural techniques of container seedlings with light medium and family selection for Phoebe bournei[J].Journal of Northwest Forestry University,26(6):70-73.]
佘萍,余治家,马杰,贾宝光,杨治科.2017.欧洲花楸育苗及幼苗生长规律试验[J].福建林业科技,44(3):94-98.[She P,Yu Z J,Ma J,Jia B G,Yang Z K.2017.Experiments on growth of seedling and seedling of Sorbus aucuparia[J].Jour of Fujian Forestry Science and Technology,44(3):94-98.]
申国柱,刘湘永,申仕康,吴艳瑞,王跃华.2008.6-BA和NAA对茶梨种子发芽特性的影响[J].种子,27(3):73-74.[Shen G Z,Liu X Y,Shen S K,Wu Y R,Wang Y H.2008.E-ffect of 6-BA and NAA on germination characteristics of Anneslea fragrans wall seed[J].Seed,27(3):73-74.]
苏文华,张光飞,张诚.2001.滇青冈种子萌发的生态生物学研究[J].种子,20(5):29-31.[Su W H,Zhang G F,Zhang C.2001.Biological and ecological studies on seed germination of Cyclobalnopsis glaucoides[J].Seed,20(5):29-31.]
王秀云,孙玉军,马炜.2011.不同密度长白落叶松林生物量与碳储量分布特征[J].福建林学院学报,3l(3):221-226.[Wang X Y,Sun Y J,Ma W.2011.Biomass and carbon storage distribution of different density in Larix olgensis plantation[J].Journal of Fujian College of Forestry,31(3):221-226.]
吴亚丽.2016.不同家系东京野茉莉种子营养成分和苗期生长差异研究[D].南京:南京林业大学.[Wu Y L.2016.Differences of seed nutrient contents,seedling growth and cold tolerance in different families of Styrax tonkinensis[D].Nanjing:Nanjing Forestry University.]
吴志树.2014.4种林木幼苗含水率与生物量的关系研究[J].现代农业科技,(21):156-159.[Wu Z S.2014.Relationship between moisture content and biomass of four species of tree seedlings[J].Modern Agricultural Sciences and Technology,(21):156-159.]
杨安敏,姚淑均,许杰,邓伯龙,尹晓阳,刘正本,王军辉.2010.滇楸半同胞家系子代苗期性状的遗传变异[J].种子,29(3):89-90.[Yang A M,Yao S J,Xu J,Deng B L,Yin X Y,Liu Z B,Wang J H.2010.The genetic variation of seeding traits among half-sib families of pius trees of Catalpa duclouxii Dode[J].Seed,29(3):89-90.]
杨志玲,杨旭,谭梓峰,甘光标,舒袅,于华会,刘若楠.2009.厚朴不同种源及家系种子性状的变异[J].中南林业科技大学学报,29(5):49-55.[Yang Z L,Yang X,Tan Z F,Gan G B,Shu N,Yu H H,Liu R N.2009.Variation of seed characters of Magnolia officinalis from different provenances and families[J].Journal of Central South University of Forestry and Technology,29(5):49-55.]
郑书绿,鲍雪纤,李莲芳,王慷林,张薇,王文俊,于国栋,郭樑,苏柠.2015.激素浸种对云南松种子发芽期地径和子叶生长的影响[J].种子,34(8):18-22.[Zheng S L,Bao X X,Li L F,Wang K L,Zhang W,Wang W J,Yu G D,Guo L,Su N.2015.Effects of hormone solutions pre-soaking seeds on diameter and cotyledon growing of Pinus yunnanensis for germinative period[J].Seed,34(8):18-22.]
中国科学院中国植物志编辑委员会.1998.中国植物志[M].北京:科学出版社.[Editorial Board of Chinese Flora of the Chinese Academy of Sciences.1998.Flora of China[M].Beijing:Science Press.]
周元.2003.滇青冈种子的萌发[J].植物生理学通讯,39(4):325-326.[Zhou Y.2003.Seed germination of Cyclobalanopsis glaucoides[J].Plant Physiology Journal,39(4):325-326.]
朱炜,李宝福,潘琼蓉,严平勇,刘正渭.2014.红锥家系苗期变异与初步选择的研究[J].山地农业生物学报,33(5):19-24.[Zhu W,Li B F,Pan Q R,Yan P Y,Liu Z W.2014.Study on variation and preliminary selection among the families of Castanopsis hystrix seedling[J].Journal of Mountain Agriculture an Biology,33(5):19-24.]
鲍雪纤,郑书绿,李莲芳,王慷林,张薇,王文俊,王文静,于国栋,郭樑,苏柠.2016.微波和激素处理对滇青冈幼苗根叶生长及生物量的影响[J].西部林业科学,45(2):83-88.[Bao X X,Zheng S L,Li L F,Wang K L,Zhang W,Wang W J,Wang W J,Yu G D,Guo L,Su N.2016.E-ffects of microwave and hormone treatments on seedling root,leaf and biomass of Cyclobalanopsis glaucoides[J].Journal of West China Forestry Science,45(2):83-88.]
曹建新,张光飞,张磊,李秀华,苏文华.2008.滇青冈幼苗的光合和生长对不同生长光强的适应[J].广西植物,28(1):126-129.[Cao J X,Zhang G F,Zhang L,Li X H,Su W H.2008.Acclimation of photosynthesis and growth to different growth light condition for seedlings of Cyclobalanopsis glaucoides[J].Guihaia,28(1):126-129.]
郭睿达,曾凡锁,詹亚光,李博,何之龙,张桂芹,姚盛智,魏志刚.2013.水曲柳优树子代测定与家系选育技术[J].东北林业大学学报,41(5):1-5.[Guo R D,Zeng F S,Zhan Y G,Li B,He Z L,Zhang G Q,Yao S Z,Wei Z G.2013.Progeny test of superior trees for Fraxinus mandshurica and pedigree breeding[J].Journal of Northeast Forestry University,41(5):1-5.]
李怀春,甘小洪,张泽鹏,张春晓,宋蔺.2015.不同海拔与母树大小对水青树种子生物学特性的影响[J].植物分类与资源学报,37(2):177-183.[Li H C,Gan X H,Zhang Z P,Zhang C X,Song L.2015.Effects of altitudes and the DBH of seed trees on biological characteristics of Tetracentron sinense(Tetracentraceae)seeds[J].Plant Diversity and Resources,37(2):177-183.]
李宁毅,苏胜举,李立.2009.GA3和6-BA对美女樱种子萌发特性及幼苗生长的影响[J].种子,28(11):59-61.[Li NY,Su S J,Li L.2009.Effects of GA3and 6-BA on the seed germination and seedling growth of Verbena hortensis[J].Seed,28(11):59-61.]
李玉文,张朝志,刘永国.2011.3种植物激素不同处理穗条对油茶扦插效果的影响[J].西部林业科学,40(3):59-62.[Li Y W,Zhang C Z,Liu Y G,2011.Effects of different treatments with 3 types of hormones on rooting of cuttage of Camellia oleifera[J].Journal of West China Forestry Science,40(3):59-62.]
刘军,姜景民,陈益泰,董汝湘,姜荣波,孔超.2011.闽楠种子轻基质容器育苗及优良家系选择[J].西北林学院学报,26(6):70-73.[Liu J,Jiang J M,Chen Y T,Dong R X,Jiang R B,Kong C.2011.Cultural techniques of container seedlings with light medium and family selection for Phoebe bournei[J].Journal of Northwest Forestry University,26(6):70-73.]
佘萍,余治家,马杰,贾宝光,杨治科.2017.欧洲花楸育苗及幼苗生长规律试验[J].福建林业科技,44(3):94-98.[She P,Yu Z J,Ma J,Jia B G,Yang Z K.2017.Experiments on growth of seedling and seedling of Sorbus aucuparia[J].Jour of Fujian Forestry Science and Technology,44(3):94-98.]
申国柱,刘湘永,申仕康,吴艳瑞,王跃华.2008.6-BA和NAA对茶梨种子发芽特性的影响[J].种子,27(3):73-74.[Shen G Z,Liu X Y,Shen S K,Wu Y R,Wang Y H.2008.E-ffect of 6-BA and NAA on germination characteristics of Anneslea fragrans wall seed[J].Seed,27(3):73-74.]
苏文华,张光飞,张诚.2001.滇青冈种子萌发的生态生物学研究[J].种子,20(5):29-31.[Su W H,Zhang G F,Zhang C.2001.Biological and ecological studies on seed germination of Cyclobalnopsis glaucoides[J].Seed,20(5):29-31.]
王秀云,孙玉军,马炜.2011.不同密度长白落叶松林生物量与碳储量分布特征[J].福建林学院学报,3l(3):221-226.[Wang X Y,Sun Y J,Ma W.2011.Biomass and carbon storage distribution of different density in Larix olgensis plantation[J].Journal of Fujian College of Forestry,31(3):221-226.]
吴亚丽.2016.不同家系东京野茉莉种子营养成分和苗期生长差异研究[D].南京:南京林业大学.[Wu Y L.2016.Differences of seed nutrient contents,seedling growth and cold tolerance in different families of Styrax tonkinensis[D].Nanjing:Nanjing Forestry University.]
吴志树.2014.4种林木幼苗含水率与生物量的关系研究[J].现代农业科技,(21):156-159.[Wu Z S.2014.Relationship between moisture content and biomass of four species of tree seedlings[J].Modern Agricultural Sciences and Technology,(21):156-159.]
杨安敏,姚淑均,许杰,邓伯龙,尹晓阳,刘正本,王军辉.2010.滇楸半同胞家系子代苗期性状的遗传变异[J].种子,29(3):89-90.[Yang A M,Yao S J,Xu J,Deng B L,Yin X Y,Liu Z B,Wang J H.2010.The genetic variation of seeding traits among half-sib families of pius trees of Catalpa duclouxii Dode[J].Seed,29(3):89-90.]
杨志玲,杨旭,谭梓峰,甘光标,舒袅,于华会,刘若楠.2009.厚朴不同种源及家系种子性状的变异[J].中南林业科技大学学报,29(5):49-55.[Yang Z L,Yang X,Tan Z F,Gan G B,Shu N,Yu H H,Liu R N.2009.Variation of seed characters of Magnolia officinalis from different provenances and families[J].Journal of Central South University of Forestry and Technology,29(5):49-55.]
郑书绿,鲍雪纤,李莲芳,王慷林,张薇,王文俊,于国栋,郭樑,苏柠.2015.激素浸种对云南松种子发芽期地径和子叶生长的影响[J].种子,34(8):18-22.[Zheng S L,Bao X X,Li L F,Wang K L,Zhang W,Wang W J,Yu G D,Guo L,Su N.2015.Effects of hormone solutions pre-soaking seeds on diameter and cotyledon growing of Pinus yunnanensis for germinative period[J].Seed,34(8):18-22.]
中国科学院中国植物志编辑委员会.1998.中国植物志[M].北京:科学出版社.[Editorial Board of Chinese Flora of the Chinese Academy of Sciences.1998.Flora of China[M].Beijing:Science Press.]
周元.2003.滇青冈种子的萌发[J].植物生理学通讯,39(4):325-326.[Zhou Y.2003.Seed germination of Cyclobalanopsis glaucoides[J].Plant Physiology Journal,39(4):325-326.]
朱炜,李宝福,潘琼蓉,严平勇,刘正渭.2014.红锥家系苗期变异与初步选择的研究[J].山地农业生物学报,33(5):19-24.[Zhu W,Li B F,Pan Q R,Yan P Y,Liu Z W.2014.Study on variation and preliminary selection among the families of Castanopsis hystrix seedling[J].Journal of Mountain Agriculture an Biology,33(5):19-24.]