基于HPLC-QTOF-MS的葛根汤化学成分表征及其不同极性部位抗RSV的谱效关系研究
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摘要
目的:研究葛根汤及不同极性部位HPLC色谱峰与其抗呼吸道合胞病毒(RSV)的谱效关系,探究药效物质。方法:应用HPLC-Q-TOF-MS技术定性色谱峰;探究各部位的体外抗RSV活性;分析HPLC色谱峰峰强度与抗RSV活性间的谱效关系;并对所得结果进行验证。结果:在标定的33个色谱峰中,有19个色谱峰与抗RSV活性呈正相关,其中7个成分贡献较大,分别为原儿茶酸、大豆苷元、芍药苷、没食子酸、葛根苷D、刺芒柄花苷和6-姜酚。进一步实验证实原儿茶酸和没食子酸具有较好的体外抗RSV活性。结论:本研究借助中药谱效学的方法初步阐释了葛根汤体外抗RSV的物质基础,为中药药效物质研究提供了一种有效的参考。
Objective: To study the relationships between HPLC peaks and anti-RSV activity of different polar parts of Ge-Gen Decoction and to explore the pharmacodynamic substances. Methods: The characteristic peaks of HPLC were qualitatively identified by HPLC-Q-TOF-MS technique. Moreover, we explored the anti-RSV activity of Ge-Gen Decoction and its different polar parts. Then, we analyzed the correlation of the HPLC peaks and the pharmacological activity of different polar parts of GeGen Decoction by partial least-squares regression analysis and validated the analytic result by experiment. Results: Among the33 characteristic peaks, 19 peaks were positively correlated with anti-RSV activity. Seven of them contributed significantly to the anti-RSV activity in vitro, including protocatechuic acid, daidzein, paeoniflorin, gallic acid, pueroside D, ononin and 6-gingerol.Furthermore, we confirmed the anti-RSV activities of protocatechuic acid and gallic. Conclusion: This study preliminarily explained the material basis of Ge-Gen Decoction against RSV in vitro by the method of the Spectrum-Efficacy of traditional Chinese medicine(TCM), which provided an effective reference for the study of the material basis of traditional Chinese medicine.
Objective: To study the relationships between HPLC peaks and anti-RSV activity of different polar parts of Ge-Gen Decoction and to explore the pharmacodynamic substances. Methods: The characteristic peaks of HPLC were qualitatively identified by HPLC-Q-TOF-MS technique. Moreover, we explored the anti-RSV activity of Ge-Gen Decoction and its different polar parts. Then, we analyzed the correlation of the HPLC peaks and the pharmacological activity of different polar parts of GeGen Decoction by partial least-squares regression analysis and validated the analytic result by experiment. Results: Among the33 characteristic peaks, 19 peaks were positively correlated with anti-RSV activity. Seven of them contributed significantly to the anti-RSV activity in vitro, including protocatechuic acid, daidzein, paeoniflorin, gallic acid, pueroside D, ononin and 6-gingerol.Furthermore, we confirmed the anti-RSV activities of protocatechuic acid and gallic. Conclusion: This study preliminarily explained the material basis of Ge-Gen Decoction against RSV in vitro by the method of the Spectrum-Efficacy of traditional Chinese medicine(TCM), which provided an effective reference for the study of the material basis of traditional Chinese medicine.
引文
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[5]殷会敏,黄文哲,肖伟.基于RRLC/Q-TOF-MS/MS技术快速分析芪葛颗粒的化学成分.中国中药杂志,2018,43(21):4240-4247
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[11]赵艳敏,刘素香,张晨曦,等.基于HPLC-Q-TOF-MS技术的甘草化学成分分析.中草药,2016,47(12):2061-2068
[12]张梅,刘静,刘军,等.LC-MS/MS分析含葛根复方制剂给药血浆中来自葛根的移行成分.中华中医药杂志,2011,26(9):1954-1957
[13]梁娜,桑亚新,周妍,等.HPLC-ESI-Q-TOF-MS/MS法分析干姜中的姜辣素类化合物.食品工业科技,2018,39(9):252-256
[14]尹权微,李家春,秦建平,等.UPLC/Q-TOF-MS快速分析桂枝茯苓胶囊的入血成分.中国实验方剂学杂志,2016,22(21):83-86
[15]刘杰,陈琳,范彩荣,等.基于HPLC-DAD-Q-TOF-MS/MS的白芍和赤芍主要成分定性定量研究.中国中药杂志,2015,40(9):1762-1770
[16]周永刚,吴思,毛飞,等.葛根中黄酮类化学成分的UHPLC-QTOFMS分析.药学实践杂志,2013,31(2):116-119
[17]赵楠.葛根总黄酮提取物中30种异黄酮的HPLC-MS鉴定.大连国际色谱学术报告会和展览会文集,2007:3
[18]朱敏,段金廒,唐于平,等.当归-白芍配伍在痛经模型大鼠体内代谢产物的研究.中华中医药杂志,2018,33(9):4098-4103
[19]王彦志,冯卫生,石任兵,等.野葛中的一个新化学成分.药学学报,2007,42(9):964-967
[2]钟慧玲,徐霖,张素粉,等.2012-2013年广州地区呼吸道合胞病毒感染的流行特征分析.热带医学杂志,2015,15(3):292-295,306
[3]Chang J S,Wang K C,Shieh D E,et al.Ge-Gen-Tang has antiviral activity against human respiratory syncytial virus in human respiratory tract cell lines.Journal of Ethnopharmacology,2012,139(1):305-310
[4]Yan Y,Chai C Z,Wang D W,et al.HPLC-DAD-Q-TOF-MS/MS analysis and HPLC quantitation of chemical constituents in traditional Chinese medicinal formula Ge-Gen Decoction.Journal of Pharmaceutical&Biomedical Analysis,2013,80(3):192-202
[5]殷会敏,黄文哲,肖伟.基于RRLC/Q-TOF-MS/MS技术快速分析芪葛颗粒的化学成分.中国中药杂志,2018,43(21):4240-4247
[6]陈永财,钱江辉,王彬辉,等.“桂枝与白芍”药对入血成分UPLC-Q/TOF-MS分析.中国现代应用药学,2017,34(12):1707-1711
[7]李晗芸,苏丹,部爱贤,等.UPLC-Q TOF MS~E与镜像对比分析四种麻黄炮制过程的成分变化.质谱学报,2017,38(6):630-639
[8]张启云,彭国梅,李冰涛,等.UHPLC-Q-TOF/MS技术分析葛根醇提液中化学成分.中药新药与临床药理,2017,28(4):513-518
[9]陈永财,钱江辉,王彬辉,等.“桂枝与白芍”药对化学成分UPLC-Q/TOF-MS分析.中国医药导报,2017,14(16):12-15,23
[10]杨彬,王媛,田梦,等.基于UPLC-Q-TOF-MS/MS研究法半夏中甘草化学成分.中国实验方剂学杂志,2017,23(3):45-49
[11]赵艳敏,刘素香,张晨曦,等.基于HPLC-Q-TOF-MS技术的甘草化学成分分析.中草药,2016,47(12):2061-2068
[12]张梅,刘静,刘军,等.LC-MS/MS分析含葛根复方制剂给药血浆中来自葛根的移行成分.中华中医药杂志,2011,26(9):1954-1957
[13]梁娜,桑亚新,周妍,等.HPLC-ESI-Q-TOF-MS/MS法分析干姜中的姜辣素类化合物.食品工业科技,2018,39(9):252-256
[14]尹权微,李家春,秦建平,等.UPLC/Q-TOF-MS快速分析桂枝茯苓胶囊的入血成分.中国实验方剂学杂志,2016,22(21):83-86
[15]刘杰,陈琳,范彩荣,等.基于HPLC-DAD-Q-TOF-MS/MS的白芍和赤芍主要成分定性定量研究.中国中药杂志,2015,40(9):1762-1770
[16]周永刚,吴思,毛飞,等.葛根中黄酮类化学成分的UHPLC-QTOFMS分析.药学实践杂志,2013,31(2):116-119
[17]赵楠.葛根总黄酮提取物中30种异黄酮的HPLC-MS鉴定.大连国际色谱学术报告会和展览会文集,2007:3
[18]朱敏,段金廒,唐于平,等.当归-白芍配伍在痛经模型大鼠体内代谢产物的研究.中华中医药杂志,2018,33(9):4098-4103
[19]王彦志,冯卫生,石任兵,等.野葛中的一个新化学成分.药学学报,2007,42(9):964-967