以PRRSV为载体表达外源基因的研究进展
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摘要
猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)是一种以呼吸系统和繁殖障碍为特征的传染病,已被公认为影响全球养猪业的重要经济性疾病。目前,传统的活疫苗免疫是防控猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)的主要手段,但该疫苗的安全性和有效性还需进一步提高。本文就对近年来以PRRSV感染性克隆作为工具表达外源基因的重组病毒的研究进展做一综述。
Porcine reproductive and respiratory syndrome characterized by respiratory and reproductive disorders in piglets and sows has spread worldwide and become the most economically significant disease. Immunization with live attenuated vaccines is the primary method for the prevention and control of PRRSV. However, the safety and efficacy of the live vaccines need further improved. This review summarizes progress of expression of foreign genes using infectious c DNA clone of PRRSV.
Porcine reproductive and respiratory syndrome characterized by respiratory and reproductive disorders in piglets and sows has spread worldwide and become the most economically significant disease. Immunization with live attenuated vaccines is the primary method for the prevention and control of PRRSV. However, the safety and efficacy of the live vaccines need further improved. This review summarizes progress of expression of foreign genes using infectious c DNA clone of PRRSV.
引文
[1]Albina E.Epidemiology of porcine reproductive and respiratory syndrome(PRRS):an overview[J].Vet Microbiol,1997,55(1-4):309-316.
[2]Kuwahara H,Nunoya T,Tajima M,et al.An outbreak of porcine reproductive and respiratory syndrome in Japan[J].J Vet Med Sci,1994,56(5):901-909.
[3]Music N,Gagono C A.The role of pocine reproductive and respiratory syndrome(PRRS)virus structural and nonstructural proteins in virus pathogenesis[J].Anim Health Res Rev,2010,11(2):135-163.
[4]Johnson CR,Griggs TF,Gnanandarajah J,et al.Novel strural protein in porcine reproductive and respiratory synndrome virus encoded by an alternative ORF5 present in all arteriviruses[J].J Gen Virol,2011,92(5):1107-1116.
[5]Goldberg T L,Lowe J F,Miburn S M,et al.Quasispecious variation of porcine reproduction and respiratory syndrome virus during natural infection[J].Virology,2003,317(2):197-207.
[6]Fang Y,Rowland R R,Roof M,et al.A full length c DNA infectious clone of north American Type 1 porcine reproductive and respiratory syndrome virus:expression of green fluorescet protein in the Nsp2 region[J].J Virol,2006,80(23):11447-11455.
[7]Xu Y Z,Zhou Y J,Zhang S R,et al.Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain(Hu N4-F112)of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell[J].Virol J,2012,9:114.
[8]Fang Y,Christopher-Hennings J,Brown E,et al.Development of genetic markers in the nonstructural protein 2 region a US Type1 procine repruductive and respiratory syndrome virus:implications for future recombinant marker vaccine development[J].J Gen Virol,2008,89(pt12):3086-3096.
[9]Wang L,Zhang K,Lin H,et al.Preparation of North American TypeⅡPRRSV Infectious clone expressing green fluorescent procine[J].Biomed Res Int,2014,368581.
[10]Han J,Liu G,Wang Y,et al.Identification of nonessential regions of the nsp2 replicase protein of porcine reproductive and respiratory syndrome virus strain VR-2332 for replication in cell culture[J].J Virol,2007,81(18):9878-9890.
[11]Xu Y Z,Zhou Y J,Zhang S R,et al.Stable expression of foreign gene in nonesential region of nonstructural protein 2(nsp2)of porcine reproductive and respiratory and respiratory syndrome virus:applications for marker vaccine design[J].Vet Microbiol,2012,159(1-2):1-10.
[12]Pei Y,Hodgins D C,Wu J,et al.Porcine reproductive and respiratory syndrome virus as a vector:Immunogenicity of green fluorescent protein and porcine circovirus type2 capsid expressed from dedicated subgenomic RNAs[J].Virology,2009,389:91-99.
[13]Sang Y M,Shi J S,Sang W J,et al.Replication competent recombinant porcine reproductive and respiratory syndrome(PRRS)viruses expressing indicator proteins and antiviral cytokines[J].Viruses,2012,4(1):102-116.
[14]Gao F,Qu Z,Li L,et al.Recombinant porcine reproductive and respiratory syndrome virus expressing luciferase genes provides a new indication of viral propagation in both Permissive and target cell(PHu N4-F112)[J].Res Vet Sci,2016,107:132-140.
[15]Zheng H,Sun Z,Zhu X Q,et al,Recombinant PRRSV expressing porcine circovirus sequence reveals novel aspect of transcriptional control of porcine arterivirus[J].Virus Res,2010,148(1-2):8-16.
[16]高飞,曲泽慧,姜一峰,等.重组猪瘟病毒C株E2蛋白的猪繁殖与呼吸综合征病毒构建及鉴定[J].中国动物传染病学报,2015,23(5):1-9.
[17]Yu L,Zhou Y,Jiang Y,et al,Construction and in vitro evalution of recombiant live attenuated PRRSV expressing GM-CSF[J].Virol J,2014,11(1):201.
[18]Wang L,Hou J,Gao L,et al.Attenuation of highly pathogenic porcine reproductive and respiratory syndrome virus by inserting an additional transcription unit[J].Vaccine,2014,32(43):5740-5748.
[19]Sun Z,Lu J,Yao H,et al.Identification of dispensable nucleotide sequence in 3'UTR of porcine reproduction and respiratory syndrome virus[J].Virus Res,2010,154(2-4):38-47.
[20]安同庆.猪繁殖与呼吸综合征病毒与宿主细胞受体之间相互作用的研究及病毒遗传变异分析[D].北京中国农业科,1999.
[21]Li Z J,Wang G,Wang Y,et al.Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4[J].Virol J,2015,12(1):185.
[22]Li Z,Wang G,Wang Y,et al.Immune responses of pigs immunized with a recombinant porcine respiratory syndrome virus expressing porcine GM-CSF[J].Vet Immunol Immunopathol,2015,168(1-2):40-48.
[2]Kuwahara H,Nunoya T,Tajima M,et al.An outbreak of porcine reproductive and respiratory syndrome in Japan[J].J Vet Med Sci,1994,56(5):901-909.
[3]Music N,Gagono C A.The role of pocine reproductive and respiratory syndrome(PRRS)virus structural and nonstructural proteins in virus pathogenesis[J].Anim Health Res Rev,2010,11(2):135-163.
[4]Johnson CR,Griggs TF,Gnanandarajah J,et al.Novel strural protein in porcine reproductive and respiratory synndrome virus encoded by an alternative ORF5 present in all arteriviruses[J].J Gen Virol,2011,92(5):1107-1116.
[5]Goldberg T L,Lowe J F,Miburn S M,et al.Quasispecious variation of porcine reproduction and respiratory syndrome virus during natural infection[J].Virology,2003,317(2):197-207.
[6]Fang Y,Rowland R R,Roof M,et al.A full length c DNA infectious clone of north American Type 1 porcine reproductive and respiratory syndrome virus:expression of green fluorescet protein in the Nsp2 region[J].J Virol,2006,80(23):11447-11455.
[7]Xu Y Z,Zhou Y J,Zhang S R,et al.Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain(Hu N4-F112)of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell[J].Virol J,2012,9:114.
[8]Fang Y,Christopher-Hennings J,Brown E,et al.Development of genetic markers in the nonstructural protein 2 region a US Type1 procine repruductive and respiratory syndrome virus:implications for future recombinant marker vaccine development[J].J Gen Virol,2008,89(pt12):3086-3096.
[9]Wang L,Zhang K,Lin H,et al.Preparation of North American TypeⅡPRRSV Infectious clone expressing green fluorescent procine[J].Biomed Res Int,2014,368581.
[10]Han J,Liu G,Wang Y,et al.Identification of nonessential regions of the nsp2 replicase protein of porcine reproductive and respiratory syndrome virus strain VR-2332 for replication in cell culture[J].J Virol,2007,81(18):9878-9890.
[11]Xu Y Z,Zhou Y J,Zhang S R,et al.Stable expression of foreign gene in nonesential region of nonstructural protein 2(nsp2)of porcine reproductive and respiratory and respiratory syndrome virus:applications for marker vaccine design[J].Vet Microbiol,2012,159(1-2):1-10.
[12]Pei Y,Hodgins D C,Wu J,et al.Porcine reproductive and respiratory syndrome virus as a vector:Immunogenicity of green fluorescent protein and porcine circovirus type2 capsid expressed from dedicated subgenomic RNAs[J].Virology,2009,389:91-99.
[13]Sang Y M,Shi J S,Sang W J,et al.Replication competent recombinant porcine reproductive and respiratory syndrome(PRRS)viruses expressing indicator proteins and antiviral cytokines[J].Viruses,2012,4(1):102-116.
[14]Gao F,Qu Z,Li L,et al.Recombinant porcine reproductive and respiratory syndrome virus expressing luciferase genes provides a new indication of viral propagation in both Permissive and target cell(PHu N4-F112)[J].Res Vet Sci,2016,107:132-140.
[15]Zheng H,Sun Z,Zhu X Q,et al,Recombinant PRRSV expressing porcine circovirus sequence reveals novel aspect of transcriptional control of porcine arterivirus[J].Virus Res,2010,148(1-2):8-16.
[16]高飞,曲泽慧,姜一峰,等.重组猪瘟病毒C株E2蛋白的猪繁殖与呼吸综合征病毒构建及鉴定[J].中国动物传染病学报,2015,23(5):1-9.
[17]Yu L,Zhou Y,Jiang Y,et al,Construction and in vitro evalution of recombiant live attenuated PRRSV expressing GM-CSF[J].Virol J,2014,11(1):201.
[18]Wang L,Hou J,Gao L,et al.Attenuation of highly pathogenic porcine reproductive and respiratory syndrome virus by inserting an additional transcription unit[J].Vaccine,2014,32(43):5740-5748.
[19]Sun Z,Lu J,Yao H,et al.Identification of dispensable nucleotide sequence in 3'UTR of porcine reproduction and respiratory syndrome virus[J].Virus Res,2010,154(2-4):38-47.
[20]安同庆.猪繁殖与呼吸综合征病毒与宿主细胞受体之间相互作用的研究及病毒遗传变异分析[D].北京中国农业科,1999.
[21]Li Z J,Wang G,Wang Y,et al.Rescue and evaluation of a recombinant PRRSV expressing porcine Interleukin-4[J].Virol J,2015,12(1):185.
[22]Li Z,Wang G,Wang Y,et al.Immune responses of pigs immunized with a recombinant porcine respiratory syndrome virus expressing porcine GM-CSF[J].Vet Immunol Immunopathol,2015,168(1-2):40-48.