摘要
目的:为明确拮抗真菌zjts7分类地位和对肿节风黑斑病菌Colletotrichum dematiu的抑菌率以及对病原菌的菌丝形态的影响。方法:本研究采用平板稀释法和平板对峙法,从健康肿节风的根系土壤里分离筛选到一株对肿节风黑斑病菌具有抑菌作用的真菌zjts7。结果:结果表明,真菌zjts7对肿节风黑斑病菌的抑菌率达到81. 82%~100%。经显微镜观察,真菌zjts7可造成肿节风黑斑病菌的菌丝断裂、膨大和畸形。结论:结合形态特征和ITS r DNA序列分析,将该拮抗真菌zjts7鉴定为棘孢木霉Trichoderma asperellum。
Objective: To clarify antagonistic fungi zjts7 classification status and the effect of strain zjts7 on the mycelia growth of the Colletotrichum dematiu was also observed under the microscope. Methods: The inhibition rates of biocontrol fungus zjts7 on C. dematiu of Sarcandra glabra from plant soil health was tested in this study by plate dilution method and plate confrontation method. Results: The result showed that strain zjts7 could inhibit C. dematiu of S. glabra with the high inhibition rates of 81. 82 % ~ 100 %. When observed under the microscope, mycelia growths of the C. dematiu was affected obviously by strain zjts7. The mycelia of C. dematiu appeared, cracked, swollen and abnormal. Conclusion: Based on morphological and rDNA-ITS sequence analysis, strain zjts7 was identified as Trichoderma asperellum.
引文
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