肿节风黑斑病拮抗真菌zjts7的抑菌作用测定与鉴定
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摘要
目的:为明确拮抗真菌zjts7分类地位和对肿节风黑斑病菌Colletotrichum dematiu的抑菌率以及对病原菌的菌丝形态的影响。方法:本研究采用平板稀释法和平板对峙法,从健康肿节风的根系土壤里分离筛选到一株对肿节风黑斑病菌具有抑菌作用的真菌zjts7。结果:结果表明,真菌zjts7对肿节风黑斑病菌的抑菌率达到81. 82%～100%。经显微镜观察,真菌zjts7可造成肿节风黑斑病菌的菌丝断裂、膨大和畸形。结论:结合形态特征和ITS r DNA序列分析,将该拮抗真菌zjts7鉴定为棘孢木霉Trichoderma asperellum。
Objective: To clarify antagonistic fungi zjts7 classification status and the effect of strain zjts7 on the mycelia growth of the Colletotrichum dematiu was also observed under the microscope. Methods: The inhibition rates of biocontrol fungus zjts7 on C. dematiu of Sarcandra glabra from plant soil health was tested in this study by plate dilution method and plate confrontation method. Results: The result showed that strain zjts7 could inhibit C. dematiu of S. glabra with the high inhibition rates of 81. 82 % ～ 100 %. When observed under the microscope, mycelia growths of the C. dematiu was affected obviously by strain zjts7. The mycelia of C. dematiu appeared, cracked, swollen and abnormal. Conclusion: Based on morphological and rDNA-ITS sequence analysis, strain zjts7 was identified as Trichoderma asperellum.
Objective: To clarify antagonistic fungi zjts7 classification status and the effect of strain zjts7 on the mycelia growth of the Colletotrichum dematiu was also observed under the microscope. Methods: The inhibition rates of biocontrol fungus zjts7 on C. dematiu of Sarcandra glabra from plant soil health was tested in this study by plate dilution method and plate confrontation method. Results: The result showed that strain zjts7 could inhibit C. dematiu of S. glabra with the high inhibition rates of 81. 82 % ～ 100 %. When observed under the microscope, mycelia growths of the C. dematiu was affected obviously by strain zjts7. The mycelia of C. dematiu appeared, cracked, swollen and abnormal. Conclusion: Based on morphological and rDNA-ITS sequence analysis, strain zjts7 was identified as Trichoderma asperellum.
引文
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[13]邵天蔚,丁万隆,李勇.人参致病菌拮抗菌株的筛选及鉴定[J].中国现代中药,2017,19(5):693-697.
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[18]陈立华,金秋,牛明,等.棘孢木霉对水稻纹枯病病原菌立枯丝核菌生物防治的研究[J].江苏农业科学,2015,43(5):115-117.
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[20]杨帅,孙丽丽,邓世林,等.棘孢木霉发酵液对番茄早疫病抗氧化系统和脂质过氧化作用的影响[J].中国农学通报,2017,33(5):90-94.
[21]覃柳燕,郭成林,黄素梅,等.棘孢木霉菌株PZ6对香蕉促生效应及枯萎病室内防效的影响[J].南方农业学报,2017,48(2):277-283.
[22]杨兴堂,吕曼曼,刘志华,等.棘孢木霉对黄花蒿叶的光合特性和产量影响[J].贵州农业科学,2016,44(1):132-136.
[23]姜传英,朱国栋,姚志红,等. 3个棘孢木霉菌株对山新杨组培移栽苗生长和光合特性的影响[J].草业科学,2016,33(6):1189-1199.
[24]贺字典,宋士清,高玉峰,等.棘孢木霉Trichoderma asperellum在土壤中定殖量的荧光定量PCR检测[J].植物保护学报,2016,43(4):552-558.
[2]国家药典委员会.中华人民共和国药典:一部[M].北京:化学工业出版社. 2005.
[3]庞声航.实用瑶药学[M].南宁:广西科学技术出版社,2008.
[4]徐志杰.草珊瑚的研究概况[J].江西中医学院学报,1994,6(1):36-37.
[5]郁建生,李英伦.草珊瑚研究进展[J].安徽农业科学,2005,33(12):2390-2392.
[6]蒋妮,唐美琼,蓝祖栽,等.肿节风炭疽病病原学研究及其对药材质量的影响[J].植物保护,2012,38(4):83-88.
[7]方中达.植病研究方法[M].北京:中国农业出版社,1998.
[8]张瑾,张树武,徐秉良,等.长枝木霉菌抑菌谱测定及其抑菌作用机理研究[J].中国生态农业学报,2014,22(6):661-667.
[9]谢晨昭,杨毅玲,李磊,等.拮抗放线菌B1菌株鉴定及其防治番茄灰霉病的初步研究[J].植物保护学报,2008,33(4):300-306.
[10]李琳,张雅梅,张祥辉,等.生防棘孢木霉T31菌株的分离筛选及其生物学特性[J].植物保护,2014,41(1):54-59.
[11]赵姣,赵蕾.一株促生拮抗木霉菌的鉴定[J].山东农业科学,2013,45(4):86-89.
[12] Mbarga J B,Hoopen G T,Adiobo J A,et al. Trichoderma asperellum:A potential biocontrol agent for Pythium myriotylum,causal agent of cocoyam(Xanthosoma sagittifolium)root rot disease in Cameroon[J]. Crop Protection,2012,36(2):18-22.
[13]邵天蔚,丁万隆,李勇.人参致病菌拮抗菌株的筛选及鉴定[J].中国现代中药,2017,19(5):693-697.
[14] Tondje P R,Roberts D P,et al. Isolation and identification of mycoparasitic isolates of Trichoderma asperellum with potential for suppression of black pod disease of cacao in Cameroon[J]. Biological Control,2007,43(2):202-212.
[15] Jiang H,Zhang L,Zhang J Z,et al. Antagonistic interaction between Trichoderma asperellum and Phytophthora capsici in vitro[J]. Journal of Zhejiang University SCIENCE B(Biomedicine&Biotechnology),2016,17(4):271-281.
[16] Trillas M I,Casanova E,Cotxarrera L,et al. Composts from agricultural waste and the Trichoderma asperellum strain T-34 suppress Rhizoctonia solani in cucumber seedlings[J].Biological Control Theory&Application in Pest Management,2006,39(1):32-38.
[17] Femández E,Segarra G,Trillas M I. Physiological effents of the induction of resistance by compost or Trichoderma asperellum strain T34 against Botrytis cinerea in tomato[J].Biological Control,2014,78(6):77-85.
[18]陈立华,金秋,牛明,等.棘孢木霉对水稻纹枯病病原菌立枯丝核菌生物防治的研究[J].江苏农业科学,2015,43(5):115-117.
[19] Wijesinghe C J,Wijieratnam R W,Samarasekara J R,et al.Development of a formulation of Trichoderma asperellum to control black rot disease on pineapple caused by(Thielaviopsis paradoxa)[J]. Crop Protection,2011,30(3):300-306.
[20]杨帅,孙丽丽,邓世林,等.棘孢木霉发酵液对番茄早疫病抗氧化系统和脂质过氧化作用的影响[J].中国农学通报,2017,33(5):90-94.
[21]覃柳燕,郭成林,黄素梅,等.棘孢木霉菌株PZ6对香蕉促生效应及枯萎病室内防效的影响[J].南方农业学报,2017,48(2):277-283.
[22]杨兴堂,吕曼曼,刘志华,等.棘孢木霉对黄花蒿叶的光合特性和产量影响[J].贵州农业科学,2016,44(1):132-136.
[23]姜传英,朱国栋,姚志红,等. 3个棘孢木霉菌株对山新杨组培移栽苗生长和光合特性的影响[J].草业科学,2016,33(6):1189-1199.
[24]贺字典,宋士清,高玉峰,等.棘孢木霉Trichoderma asperellum在土壤中定殖量的荧光定量PCR检测[J].植物保护学报,2016,43(4):552-558.